RchiOBHm_Chr2g0092861

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008827
Physical Location & Seq
Reverse (-)
6247997 .. 6253322
5326 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46793

Sequence Viewer

Length: 3600 bp
ATGCTTCACAGGAGCTTCAAGCCCGCCAAATGCAAAACGGCGTTGAAGCTTGCCGTTTCGAGAATCAAGCTGTTGAAGAATAAGAAAGATGCCCAGGTGAAGCAGATAAAGAGGGAATTGGCCCAATTGCTGGAGAGTGGTCAGTACAGGACTGCTAGGATTCGGGTTGAACATGTGGTGAGGGAAGAGAAGTTGAGGGCAGCGTATGAACTTATCGAGATATATTGCGAACTTGTTGCAGCACGATTGCCTATGGTTGAATCGCAGAAAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGACGTACCAGAACTCATGGATATCCGCAAGCACTTCACAGCAAAATACGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGGCCGGATAGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCGGATGGCCCTACCAAGATGAAAATTTTGGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGCACGGACCAAATACGTTTGGAATGGCCAGTATGGATTCTGCTCCACCTCCACCTGCTCATGATGTCAGGGGACCTCCTAATGTCCAAGTTCCACCTAATAGCATGGCCAGTAAAGCTCATGTGGAACCTCCCTCTGGTCCACCTCCACCTGATCAACCTCCAATTGCTCAAGTTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACCTCACTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGAAACCAGAAGCAGTCGAACGGAAATGCATGTATTTCCGGGGGATGAAAGTTCTTCGGGCAGGCAGAATTGGAATATGCAATTTAAGGATGCTGCATCTGCAGCACAGGCAGCTGCTGAATCAGCAGAACTAGCAGGCATGGCAGCCAGAGCAGCTGCAGAACTCTCGAGGCAATACTCATCTGAAATGCACAATAAAAGTAATCAGTCAGCCTCTGTGCCAACTAATTCAAGTTCCATGAACAATGACCATTTGGGGAATAGTCTCCAGACGGCAGATCGATATCCTCAAAAGAAGTCACCTGAACGTCATGACATTTTAGATAAGGCAAGCATGAAGAGACAGTCTGGTAATTCTGAGGATATGCATGCAAGTGAGATGCAAACTGCTATCAAACCTGAGAATATTCCTTATTTTGGAGATATAAGATCTGAAAAGAAATCTAGCAGGACTTCCTCCCCATCTAATTCAAGTGTTGGTAGCGATCATCTGGAAGACGTTTTGAAAGAGGATGATCATATTAATTACTTTGCAGATCTGGGTACTGGAAAACAATCTATCAGGTCTTCCTCTCCTTCTCATTCAGGACATTTTAGTAACAATCACGAAAATTTCGCTAGTGTTTCTGGTGAGGGTTCTTTTGTTAGTGATGTTAACATCCATCAAAGCACAATTGGAATGAATTCTGAGGGAAATGCTCCTCTGGTTTTTGATGATTCTGGTTCAGATGATGATAATTACAAGGGACAAGAATCAAGTTTGTTTTTTTCTTCTCCTGCTAGAAATTCATTTCCTTCAGCTACTAGTTTAGATCCTTGGAGCTTTGAGCAAAAGAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGGGCAGCACTCCCTTCCCATCTTTTCTGAAGGTTTGACAGGTTCTACAGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCTGACAGTGAGGAGGAGTTGGACACGTCTAAGCTTGTCCTGAGCAAAGATTCTAAATTTTCTCATGAGAAGATTGTTCAATCCATAAATTCAGGAAAGAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCCTCTGATGAGGTACATCCTGAGAGAACCCAGGGAATTGAATATAGTGTTTTGTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGTAGTGAACCATCTCCGAGGCTGAAAAAATCTGGATTAGATACAAGTGCCAAGGATGATTTACCTCAAACAGTGAAAGACGGTGAGGAATGTAGTTGTGCAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGTGGCCTTCGGAACAAGGGTTATAGGCATCCACCATATAATCGGAATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATACTCCAACAGTCAGGACTTCCGATGTTTCTCATACGTCTATTGAAGAGCCATATAGCCGGAAAGGGAGCACTAAGCTAAGTAAGGAACAAAGCGCTAAAACCCCAGCCACACATGTCACTCCTGATGATGATTCGTCAGAGGATGAACTCTCACATGAAACTCTAAGCAGTAGTCGCCAAGACCTGTACAATCAGAAATTGGTCAATCGAGTAGTACATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCGAGACCCATCCCATCATAAATTGGAATCCCAAGGAAAGACAAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGATAGTGAGGCTGAGGAAGATGTTCCTAGAAAGATTTCCACCACCATTGCTCGTCCAAGTTCAAAATTTTCTAGAAGGACACAACCCTCTTCTTCAAATTCTGTTAGAAGTTCTAGCATAAGGACAACAGTTGTTCCTGATGCACCAAGGACTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAATGACGCTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAGAACAAACAAGTTCTTATTCAATTCCAGAACCCAAGAAGACTGGGACATCAGTCAATGGAAATTCCTCCTCAAGAAGTTCTTATGCCACTGAAACTCTACCAAGTGCCTCATCCCAGACCAAGCGCTCAGACCGTTCGGCAAGTCAGGAGTGGCGTAGACCAGTAGAACAAGCAGCACCTAAACCAATACCAGAATCTAAGAGGTCCTCGCGTGTGGAGACTTCAAACTTATTTCCAAGGGAGAAATCATCCAATACTCCAATGACTGTAAGATCAGAGAGAACTGGAGCTACTGAAACTTCAAAGTCCTTGGGTTCAAAGTCATTTGGAAGAGAACAATCATCTAATCCTCCAAAGATTGAAAGATCAGAAAGCAGCGAAAGTTCAAAGTCCTCGGGTTTTAAGTCATTTGCAAGAGACCAATCGTCCAATCTTCCTGCAAAGGTTGTAAGATCACAGAGAAGTGACCCTTCAAAGTTCTCTCCTCCGAAGTCATTTACAAGGGACCAAACTTCCAATCCTCCTCCAAAGATTGTAAGATCAGAGAGCGCTGAAACTCCGAAGTCCTCTTCTTCAGTGGGGAAGGCTTCTCATGTCCACCCGAAGCTTCCCGACTCTGATGACTTGTTTGCCAAGTTTACAGCCCTCAAGCAGAATCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1199

Amino Acids

131.3

Weight (kDa)

6.34

Isoelectric Point (pI)

66.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 4.3e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 634
Acc36I ACCTGC 2 cut(s) 576, 634
Acc65I GGTACC 1 cut(s) 1744
AccB1I GGYRCC 1 cut(s) 1744
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 3091
AccII CGCG 1 cut(s) 3145
AciI CCGC 5 cut(s) 24, 332, 358, 415, 436
AclWI GGATC 3 cut(s) 527, 540, 1706
AcoI YGGCCR 3 cut(s) 416, 597, 678
AcuI CTGAAG 8 cut(s) 308, 882, 1680, 1830, 1860, 2559, 2829, 3492
AfaI GTAC 7 cut(s) 146, 339, 1444, 1746, 2040, 2534, 2562
AfeI AGCGCT 3 cut(s) 2440, 3059, 3484
AfiI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
AflIII ACRYGT 3 cut(s) 172, 1914, 2458
AhdI GACNNNNNGTC 1 cut(s) 3358
AhlI ACTAGT 1 cut(s) 1703
AjiI CACGTC 1 cut(s) 1917
AjnI CCWGG 2 cut(s) 93, 2055
AjuI GAANNNNNNNTTGG 2 cut(s) 2902, 2934
Alw21I GWGCWC 1 cut(s) 2417
Alw26I GTCTC 7 cut(s) 400, 1169, 1234, 2613, 2920, 3146, 3345
AlwI GGATC 3 cut(s) 527, 540, 1706
AlwNI CAGNNNCTG 2 cut(s) 1016, 1115
Ama87I CYCGRG 3 cut(s) 536, 1066, 3328
Aor51HI AGCGCT 3 cut(s) 2440, 3059, 3484
AoxI GGCC 8 cut(s) 120, 416, 475, 597, 678, 1882, 2238, 2260
AseI ATTAAT 1 cut(s) 1422
Asp700I GAANNNNTTC 3 cut(s) 1582, 2712, 2725
Asp718I GGTACC 1 cut(s) 1744
AspLEI GCGC 3 cut(s) 2441, 3060, 3485
AsuC2I CCSGG 2 cut(s) 939, 2112
AsuHPI GGTGA 8 cut(s) 109, 190, 459, 804, 1191, 1541, 1755, 2207
AvaI CYCGRG 3 cut(s) 536, 1066, 3328
AvaII GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3138, 3439
AxyI CCTNAGG 1 cut(s) 561
BalI TGGCCA 2 cut(s) 599, 680
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 1744
BanII GRGCYC 1 cut(s) 2904
BbsI GAAGAC 3 cut(s) 1401, 1458, 2978
Bbv12I GWGCWC 1 cut(s) 2417
BbvCI CCTCAGC 1 cut(s) 2703
BceAI ACGGC 3 cut(s) 38, 54, 1188
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 1047, 1081
BciT130I CCWGG 2 cut(s) 95, 2057
BclI TGATCA 2 cut(s) 724, 1414
BcnI CCSGG 2 cut(s) 939, 2112
BcoDI GTCTC 7 cut(s) 400, 1169, 1234, 2613, 2920, 3146, 3345
BcuI ACTAGT 1 cut(s) 1703
BfmI CTRYAG 5 cut(s) 399, 999, 1056, 1827, 2931
BfoI RGCGCY 3 cut(s) 2442, 3061, 3486
BfuAI ACCTGC 2 cut(s) 576, 634
BglII AGATCT 2 cut(s) 1328, 1435
Bme1390I CCNGG 4 cut(s) 95, 939, 2057, 2112
Bme18I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3138, 3439
BmeRI GACNNNNNGTC 1 cut(s) 3358
BmeT110I CYCGRG 3 cut(s) 536, 1066, 3328
BmgBI CACGTC 1 cut(s) 1917
BmiI GGNNCC 6 cut(s) 534, 645, 699, 1746, 1842, 3440
BmrFI CCNGG 4 cut(s) 95, 939, 2057, 2112
BmrI ACTGGG 1 cut(s) 2985
BmsI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1861, 2027, 2293, 2821
BmuI ACTGGG 1 cut(s) 2985
BoxI GACNNNNGTC 1 cut(s) 2984
BpiI GAAGAC 3 cut(s) 1401, 1458, 2978
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 3 cut(s) 152, 1151, 3240
Bpu10I CCTNAGC 2 cut(s) 1931, 2703
BpuEI CTTGAG 5 cut(s) 726, 2325, 2890, 2989, 3566
BpuMI CCSGG 2 cut(s) 939, 2112
Bsa29I ATCGAT 1 cut(s) 1180
BsaBI GATNNNNATC 1 cut(s) 1182
BsaI GGTCTC 3 cut(s) 2613, 2920, 3345
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
Bse1I ACTGG 6 cut(s) 600, 681, 1450, 2980, 3095, 3223
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 2320, 2736
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 2 cut(s) 95, 2057
BseCI ATCGAT 1 cut(s) 1180
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
BseMI GCAATG 2 cut(s) 2320, 2736
BseMII CTCAG 8 cut(s) 552, 1248, 1290, 1578, 1922, 2037, 2694, 3075
BseNI ACTGG 6 cut(s) 600, 681, 1450, 2980, 3095, 3223
BseRI GAGGAG 6 cut(s) 1590, 1916, 1919, 2992, 3408, 3447
BseX3I CGGCCG 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 2449
BsgI GTGCAG 1 cut(s) 557
Bsh1236I CGCG 1 cut(s) 3145
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1884, 2240, 2262
BshNI GGYRCC 1 cut(s) 1744
BshVI ATCGAT 1 cut(s) 1180
BsiEI CGRYCG 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 2417
BsiHKCI CYCGRG 3 cut(s) 536, 1066, 3328
BsiSI CCGG 5 cut(s) 419, 470, 938, 2112, 2404
BslFI GGGAC 5 cut(s) 657, 1659, 1817, 2992, 3452
BslI CCNNNNNNNGG 4 cut(s) 130, 425, 707, 1316
BsmAI GTCTC 7 cut(s) 400, 1169, 1234, 2613, 2920, 3146, 3345
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 5 cut(s) 657, 1659, 1817, 2992, 3452
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1884, 2240, 2262
Bso31I GGTCTC 3 cut(s) 2613, 2920, 3345
BsoBI CYCGRG 3 cut(s) 536, 1066, 3328
Bsp1286I GDGCHC 2 cut(s) 2417, 2904
Bsp1407I TGTACA 1 cut(s) 2532
BspACI CCGC 5 cut(s) 24, 332, 358, 415, 436
BspANI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1884, 2240, 2262
BspCNI CTCAG 8 cut(s) 553, 1249, 1291, 1579, 1923, 2038, 2695, 3074
BspDI ATCGAT 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 3145
BspHI TCATGA 3 cut(s) 631, 1210, 1954
BspLI GGNNCC 6 cut(s) 534, 645, 699, 1746, 1842, 3440
BspMAI CTGCAG 3 cut(s) 403, 1003, 1060
BspMI ACCTGC 2 cut(s) 576, 634
BspPI GGATC 3 cut(s) 527, 540, 1706
BspQI GCTCTTC 1 cut(s) 2385
BspT107I GGYRCC 1 cut(s) 1744
BspTNI GGTCTC 3 cut(s) 2613, 2920, 3345
BsrDI GCAATG 2 cut(s) 2320, 2736
BsrGI TGTACA 1 cut(s) 2532
BsrI ACTGG 6 cut(s) 600, 681, 1450, 2980, 3095, 3223
BssT1I CCWWGG 6 cut(s) 1715, 2163, 2647, 2837, 3170, 3243
Bst2UI CCWGG 2 cut(s) 95, 2057
Bst4CI ACNGT 9 cut(s) 1245, 1831, 1898, 2185, 2195, 2356, 2821, 3068, 3202
Bst6I CTCTTC 8 cut(s) 180, 543, 1232, 2385, 2785, 2869, 3259, 3508
BstAUI TGTACA 1 cut(s) 2532
BstC8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 2002
BstFNI CGCG 1 cut(s) 3145
BstH2I RGCGCY 3 cut(s) 2442, 3061, 3486
BstHHI GCGC 3 cut(s) 2441, 3060, 3485
BstMAI GTCTC 7 cut(s) 400, 1169, 1234, 2613, 2920, 3146, 3345
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 2 cut(s) 95, 2057
BstNSI RCATGY 5 cut(s) 176, 442, 932, 1271, 2462
BstPAI GACNNNNGTC 1 cut(s) 2984
BstSCI CCNGG 4 cut(s) 93, 937, 2055, 2110
BstSFI CTRYAG 5 cut(s) 399, 999, 1056, 1827, 2931
BstUI CGCG 1 cut(s) 3145
BstV2I GAAGAC 3 cut(s) 1401, 1458, 2978
BstX2I RGATCY 4 cut(s) 532, 1328, 1435, 1711
BstYI RGATCY 4 cut(s) 532, 1328, 1435, 1711
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1180
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1884, 2240, 2262
BsuTUI ATCGAT 1 cut(s) 1180
BtrI CACGTC 1 cut(s) 1917
BtsIMutI CAGTG 4 cut(s) 1903, 2190, 3021, 3516
BveI ACCTGC 2 cut(s) 576, 634
Cac8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 2002
CaiI CAGNNNCTG 2 cut(s) 1016, 1115
CciI TCATGA 3 cut(s) 631, 1210, 1954
CfoI GCGC 3 cut(s) 2441, 3060, 3485
ClaI ATCGAT 1 cut(s) 1180
CseI GACGC 1 cut(s) 2891
Csp6I GTAC 7 cut(s) 145, 338, 1443, 1745, 2039, 2533, 2561
CspCI CAANNNNNGTGG 2 cut(s) 2719, 2754
CviQI GTAC 7 cut(s) 145, 338, 1443, 1745, 2039, 2533, 2561
DriI GACNNNNNGTC 1 cut(s) 3358
EaeI YGGCCR 3 cut(s) 416, 597, 678
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 8 cut(s) 180, 543, 1232, 2385, 2785, 2869, 3259, 3508
Eam1105I GACNNNNNGTC 1 cut(s) 3358
EarI CTCTTC 8 cut(s) 180, 543, 1232, 2385, 2785, 2869, 3259, 3508
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 6 cut(s) 1715, 2163, 2647, 2837, 3170, 3243
Eco24I GRGCYC 1 cut(s) 2904
Eco31I GGTCTC 3 cut(s) 2613, 2920, 3345
Eco32I GATATC 2 cut(s) 355, 1184
Eco47I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3138, 3439
Eco47III AGCGCT 3 cut(s) 2440, 3059, 3484
Eco52I CGGCCG 1 cut(s) 416
Eco57I CTGAAG 8 cut(s) 308, 882, 1680, 1830, 1860, 2559, 2829, 3492
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 3 cut(s) 536, 1066, 3328
EcoO109I RGGNCCY 3 cut(s) 565, 644, 3138
EcoRI GAATTC 2 cut(s) 784, 1582
EcoRII CCWGG 2 cut(s) 93, 2055
EcoRV GATATC 2 cut(s) 355, 1184
EcoT14I CCWWGG 6 cut(s) 1715, 2163, 2647, 2837, 3170, 3243
EcoT22I ATGCAT 2 cut(s) 930, 1269
EcoT38I GRGCYC 1 cut(s) 2904
ErhI CCWWGG 6 cut(s) 1715, 2163, 2647, 2837, 3170, 3243
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 6 cut(s) 2247, 2279, 2998, 3030, 3388, 3420
FaqI GGGAC 5 cut(s) 657, 1659, 1817, 2992, 3452
FauI CCCGC 1 cut(s) 31
FbaI TGATCA 2 cut(s) 724, 1414
FblI GTMKAC 1 cut(s) 3091
FriOI GRGCYC 1 cut(s) 2904
GlaI GCGC 3 cut(s) 2440, 3059, 3484
GsaI CCCAGC 1 cut(s) 2453
GsuI CTGGAG 3 cut(s) 152, 1151, 3240
HaeII RGCGCY 3 cut(s) 2442, 3061, 3486
HaeIII GGCC 8 cut(s) 122, 418, 477, 599, 680, 1884, 2240, 2262
HapII CCGG 5 cut(s) 419, 470, 938, 2112, 2404
HgaI GACGC 1 cut(s) 2891
HhaI GCGC 3 cut(s) 2441, 3060, 3485
Hin6I GCGC 3 cut(s) 2439, 3058, 3483
HinP1I GCGC 3 cut(s) 2439, 3058, 3483
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 3 cut(s) 47, 1922, 3539
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 5 cut(s) 419, 470, 938, 2112, 2404
HphI GGTGA 8 cut(s) 109, 190, 459, 804, 1191, 1541, 1755, 2207
Hpy166II GTNNAC 6 cut(s) 713, 1555, 2120, 3092, 3532, 3573
Hpy8I GTNNAC 6 cut(s) 713, 1555, 2120, 3092, 3532, 3573
HpyCH4III ACNGT 9 cut(s) 1245, 1831, 1898, 2185, 2195, 2356, 2821, 3068, 3202
HpyCH4IV ACGT 8 cut(s) 336, 587, 1207, 1398, 1771, 1916, 2381, 2666
HpySE526I ACGT 8 cut(s) 336, 587, 1207, 1398, 1771, 1916, 2381, 2666
HspAI GCGC 3 cut(s) 2439, 3058, 3483
KpnI GGTACC 1 cut(s) 1748
Ksp22I TGATCA 2 cut(s) 724, 1414
KspAI GTTAAC 1 cut(s) 1555
LguI GCTCTTC 1 cut(s) 2385
LmnI GCTCC 6 cut(s) 12, 619, 1603, 1719, 2412, 3221
LweI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1861, 2027, 2293, 2821
MaeII ACGT 8 cut(s) 336, 587, 1207, 1398, 1771, 1916, 2381, 2666
MaeIII GTNAC 5 cut(s) 1197, 1496, 1861, 2461, 3398
MfeI CAATTG 5 cut(s) 125, 279, 504, 735, 1572
MflI RGATCY 4 cut(s) 532, 1328, 1435, 1711
MhlI GDGCHC 2 cut(s) 2417, 2904
MlsI TGGCCA 2 cut(s) 599, 680
MluNI TGGCCA 2 cut(s) 599, 680
MlyI GAGTC 1 cut(s) 3542
MmeI TCCRAC 2 cut(s) 1890, 2375
Mox20I TGGCCA 2 cut(s) 599, 680
Mph1103I ATGCAT 2 cut(s) 930, 1269
MroXI GAANNNNTTC 3 cut(s) 1582, 2712, 2725
MscI TGGCCA 2 cut(s) 599, 680
MseI TTAA 6 cut(s) 443, 984, 1422, 1554, 2682, 3336
MslI CAYNNNNRTG 1 cut(s) 1272
Msp20I TGGCCA 2 cut(s) 599, 680
MspA1I CMGCKG 3 cut(s) 404, 1013, 1055
MspI CCGG 5 cut(s) 419, 470, 938, 2112, 2404
MspR9I CCNGG 4 cut(s) 95, 939, 2057, 2112
MunI CAATTG 5 cut(s) 125, 279, 504, 735, 1572
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 2 cut(s) 95, 2057
MvnI CGCG 1 cut(s) 3145
NciI CCSGG 2 cut(s) 939, 2112
NlaIV GGNNCC 6 cut(s) 534, 645, 699, 1746, 1842, 3440
NmeAIII GCCGAG 1 cut(s) 2642
NmuCI GTSAC 3 cut(s) 1197, 2461, 3398
NsiI ATGCAT 2 cut(s) 930, 1269
NspI RCATGY 5 cut(s) 176, 442, 932, 1271, 2462
PaeI GCATGC 1 cut(s) 1271
PaeR7I CTCGAG 1 cut(s) 1066
PagI TCATGA 3 cut(s) 631, 1210, 1954
PaqCI CACCTGC 1 cut(s) 634
PasI CCCWGGG 1 cut(s) 2056
PciI ACATGT 2 cut(s) 172, 2458
PciSI GCTCTTC 1 cut(s) 2385
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 3 cut(s) 1582, 2712, 2725
PflMI CCANNNNNTGG 1 cut(s) 130
PleI GAGTC 1 cut(s) 3542
PpsI GAGTC 1 cut(s) 3542
PpuMI RGGWCCY 3 cut(s) 565, 644, 3138
PscI ACATGT 2 cut(s) 172, 2458
PshAI GACNNNNGTC 1 cut(s) 2984
PshBI ATTAAT 1 cut(s) 1422
Psp5II RGGWCCY 3 cut(s) 565, 644, 3138
Psp6I CCWGG 2 cut(s) 93, 2055
PspFI CCCAGC 1 cut(s) 2449
PspGI CCWGG 2 cut(s) 93, 2055
PspN4I GGNNCC 6 cut(s) 534, 645, 699, 1746, 1842, 3440
PspPPI RGGWCCY 3 cut(s) 565, 644, 3138
PsrI GAACNNNNNNTAC 2 cut(s) 3208, 3240
PstI CTGCAG 3 cut(s) 403, 1003, 1060
PstNI CAGNNNCTG 2 cut(s) 1016, 1115
PsuI RGATCY 4 cut(s) 532, 1328, 1435, 1711
PvuII CAGCTG 3 cut(s) 404, 1013, 1055
RsaI GTAC 7 cut(s) 146, 339, 1444, 1746, 2040, 2534, 2562
RsaNI GTAC 7 cut(s) 145, 338, 1443, 1745, 2039, 2533, 2561
RseI CAYNNNNRTG 1 cut(s) 1272
SapI GCTCTTC 1 cut(s) 2385
SaqAI TTAA 6 cut(s) 443, 984, 1422, 1554, 2682, 3336
SchI GAGTC 1 cut(s) 3542
ScrFI CCNGG 4 cut(s) 95, 939, 2057, 2112
SduI GDGCHC 2 cut(s) 2417, 2904
SfaNI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1861, 2027, 2293, 2821
SfcI CTRYAG 5 cut(s) 399, 999, 1056, 1827, 2931
Sfr274I CTCGAG 1 cut(s) 1066
SinI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3138, 3439
SlaI CTCGAG 1 cut(s) 1066
SmiMI CAYNNNNRTG 1 cut(s) 1272
SmlI CTYRAG 6 cut(s) 741, 1066, 2340, 2905, 3004, 3581
SmoI CTYRAG 6 cut(s) 741, 1066, 2340, 2905, 3004, 3581
SpeI ACTAGT 1 cut(s) 1703
SphI GCATGC 1 cut(s) 1271
SsiI CCGC 5 cut(s) 24, 332, 358, 415, 436
SspI AATATT 1 cut(s) 1306
StyD4I CCNGG 4 cut(s) 93, 937, 2055, 2110
StyI CCWWGG 6 cut(s) 1715, 2163, 2647, 2837, 3170, 3243
TaaI ACNGT 9 cut(s) 1245, 1831, 1898, 2185, 2195, 2356, 2821, 3068, 3202
TaiI ACGT 8 cut(s) 339, 590, 1210, 1401, 1774, 1919, 2384, 2669
TaqI TCGA 7 cut(s) 59, 216, 916, 1067, 1180, 2554, 3592
TatI WGTACW 3 cut(s) 144, 2532, 2560
TauI GCSGC 2 cut(s) 418, 438
Tru1I TTAA 6 cut(s) 443, 984, 1422, 1554, 2682, 3336
Tru9I TTAA 6 cut(s) 443, 984, 1422, 1554, 2682, 3336
TscAI CASTG 4 cut(s) 1903, 2190, 3028, 3516
TseFI GTSAC 3 cut(s) 1197, 2461, 3398
Tsp45I GTSAC 3 cut(s) 1197, 2461, 3398
TspGWI ACGGA 4 cut(s) 396, 591, 935, 1747
TspRI CASTG 4 cut(s) 1903, 2190, 3028, 3516
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3138, 3439
VspI ATTAAT 1 cut(s) 1422
XbaI TCTAGA 1 cut(s) 2762
XceI RCATGY 5 cut(s) 176, 442, 932, 1271, 2462
XhoI CTCGAG 1 cut(s) 1066
XmiI GTMKAC 1 cut(s) 3091
XmnI GAANNNNTTC 3 cut(s) 1582, 2712, 2725
Zsp2I ATGCAT 2 cut(s) 930, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.