RLG00000016336

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008827
Physical Location & Seq
Reverse (-)
5742486 .. 5747178
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016336

Sequence Viewer

Length: 3597 bp
ATGCTTCACAGGAGCTTCAAGCCCGCCAAATGCAAAACGGCGTTGAAGCTTGCCGTTTCGAGAATAAAGCTGTTGAAGAATAAGAAAGATGCCCAGGTGAAGCAGATAAAGAGGGAATTGGCCCAATTGCTGGAGAGTGGTCAGTACAGGACTGCTAGGATTCGGGTTGAACATGTGGTGAGGGAAGAGAAGTTGAGGGCAGCGTATGAACTTATCGAGATATATTGCGAACTTGTTGCAGCACGATTGCCTATGGTTGAATCTCAGAAAAACTGCCCAATTGATCTGAAGGAAGCAGTATCAAGTGTAATTTTTGCATCTCCTAGATGTGCGGATGTACCAGAACTCATGGATATCCGCAAGCACTTCACAGCAAAATATGGAAAGGAGTTCGTCTCTGCAGCTGTTGAACTGCGGCCGGATTGTGGTGTAAGCCGCATGTTAATAGAGAAATTATCTACCAAATCACCTGATGGCCCAACCAAGATGAAAATTTTGGCTGCAATTGCAGAGGAACATAATGTCAAATGGGATCCCGAGGCATTTGAAGAGAAGCCTCCTGAGGACCTGCTGAACGGACCGAATACGTTTGGAATGGCCAGTATGGATTCTGCTCCACCTCCACCTACTCATGATGTAAGGGGACCTCCTAATGCCCAAGTTCCGCCTAATAGCATGGCCAGTAAACCTCATGTGGAACCTCCCTCTGGTCCACCTCCACCTGATCAACCTCCTATTGCTCAAGTTCCACGCAGAAATTATGAGTATCACGATGGACCTATGAATTCTAATGAGCAGAATGCAAGATCATCACCTCACTTGGAGGATTCTGCTTCTACAAATGTTTCTGCCAACAAAGCAACCACATCTGCCAAATTTCATCCAGAAACGACATCTTCAGAAACCAGAAGTGATCAAAAGGAAATGCATGTATTTCCTGGGGATGAAAGTTCTTCGGGCAGGCAGAATTGGAATATGCAATTTAAGGATGCTGCATCTGCAGCACAAGCAGCTGCTGAATCAGCAGAACTAGCAGGCATGGCAGCCAGAGCAGCTGCAGAACTCTCGAGGCAATATTCATCTGAAATGCACAATAAAAGTAATCAGTCAGCCTCTGTGCCAACTAATTCAAGTTCCATGAACAATGACCTTTTGGGGAATAGTCTCCAGACAGCAGATCGATATCCTCAAAAGAAGTCACCTGAACGTCATGACATTTTAGATAAGGCGAGCATGACGAGACAGTCCGGTAATTCTGAGGATATGCATGCAACTGAGATGCAAACTGCTATCAAACCTGAGAATATTTCTTATTTTGGAGATATAAGATCTGAAAAGAAATCTAGCAGGACTTCCTCCCCATCTAATTCAAGTGTTGGTAGCGATCGTCAGGAAGACGTTTTGAAAGAGGATGATCATATTAATTACTTTGCAGATCTGGGTACTGGAAAACAATCTATCAGGTCTTCCTCTCCTTCTCATTCAGGACATTTTAGTAACAATCACGAAAATTTCGCTAGTGTTTCTGGTGAGGGTTCTTTTGTTAGTGATGTTAACATCCATCAAAGCACAATTGGAATGAATTCTGAGGGAAATGCTCCTCTGGTTTTTGATGATTCTGGTTCAGACGATGATAATTACATGGGACAAGAATCAAGTTTGTTTTTTTCTTCTCCTGCTAGAAATTCATTTCCTTCAGCTAGTTTAGATCCTTGGAGCTTTGAGCAAAAGAGAGATGAGGTACCATTCAAGTCCGTTTCACCATTACGTTCTTCATCAGTGCAGCACTCCCTTTCCATCTTTTCTGAAGGTTTGACAGGTTCTACAGTCCCTTCGGAACCAAATGACTTCGTGCCTGTTACTTTTGATGCTTCAGATGGCCCAAGTTCAGACAGTGAGGAGGAGTTGGACACGTCTATGCTTGTCCTGAGTAAAGATTCTAAATTTTCTCATGAGAAGACTGTTCAATCCATAAATTCGGGAAAGAGCCAGAGTGCAAGCCACAGGTCATTAGCATCTTCATCTTCTGATGAGGTACATCCTGAGAGAACCCAGGGAATTGAATATAGTGTTTTCTCAGATAAAAGGTTTGATGATGATGAATTGCCCGGTAGTGAACCATCTCCAAGGCTGAAAAAATCTGGATTAGATACAAGCGCCAAGGATGATTTACCTCAAACAGTGAAAGACAGTGAGGAATGTAGTTGTGTAAGTGATACTGACAATGAGTTGTTGGCCTTTGGGGTCTTGAAAGGTGGCCTTCGGAACAAGGGTTATAGGCATCCACCATATAATCGGAATCCATCAGGCAATGCTTTTTTGGGCAAACAAATTACTCAAGATACTCCAACAGTCAGGACTTCCAATGTTTCTCATACGCCTATTGAAGAGCCATATAGCCGGAAAGGGAGCACTAAGCTAAATAAGGAACAAAGCACTAAAACCCCAGTCACACATGTCACTCCTGATGATGATTCTTCAGAGGATGAACTCTCACATGAAACTCTAAGCAGTAGTCACCAAGACCTGTACAATCAGAAATTGGTCTCTCGAGTAGTACATGATGATTCTTCTGGGGATGAACTCTCACATGAAACTCTTAGCAATAGTCGCCAAGACCTGTCCCATCATAAATCGGTATCCCAAGGAAAGACAAGCTCAACGTCTGCATTTTTCTTTAATTCAGAGGATAGTGAGGCTGAGGAAGATGTTCCTAGAAAGATTTCCACCACCATTGCTCGTCCAAGTTCAAAATTTTCTAGAAGGACACAACCCTCTTCTTCAAATTCTGTTAGAAGTTCTAGCATAAGGACAACGGTTGTTCCTGATGCACGAAGGACTTCAGACTATGGAAAATCCTCTTCAAGAAGTTCCAATGACACTGAAACTCTACCAGAGCCCTCAAGAAGTCAGGATTGGCAGAGACCTATAGAACAAATAAGTTCTTATTCAATTCCAGAATCCAAGAAGACTGGGACATCAGTCAATGGAAATTCCTCCTCAAGAAGTTCTTATGCTACTGAAACTCTACCAAGCGCCTCATCCCAGACCGAGCGCTCAGAACGTTTGGCAAGTCAGGAGTGGCGTAGACCAGTAGAACAAGCTGCACCTAAACCAATACCAGAATCTAAGAGGTCCTCGCGTGTGGAGACTTCAAACTCATTTGCAAGGGAGGAATCATCCAATACTCCAATGACTGTAAGATCAGAGAGAACTGAAGCTACTGAAACTTCAAAGTCCTTGGGTTCAAAGTCATTTGGAAGAGAACAATCATCTAATCCTCCAAAGATTGAAAGATCAGAAAGCAGCGAAAGTTCAAAGTCCTCGGGTTTTAAGTCATTTGCAAGAGACCAATCGTCCAATCTTCCTGCAAAGGTTGTAAGATCACAGAGAACTGACCCTTCGAAGTTCTCTCCTCCAAAGTCATTTACAAGGGACCAAACTTCCAATCCTCCCCCAAAGATTGTAAGATCAGAGAGCGCTGAAACTCCGAAGTCCTCTTCTTCAGTGGGGAAGGCTTCTCATGTCCACCCGAAGCTTCCCGACTCTGATGACTTGTTTGCCAAGTTTACAGCCCTCAAGCAGAATCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

1199

Amino Acids

131.43

Weight (kDa)

6.05

Isoelectric Point (pI)

66.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 12 - 176 2.2e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1243
Acc36I ACCTGC 1 cut(s) 576
Acc65I GGTACC 1 cut(s) 1741
AccB1I GGYRCC 1 cut(s) 1741
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 3088
AccII CGCG 1 cut(s) 3142
AciI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
AclI AACGTT 1 cut(s) 3064
AclWI GGATC 3 cut(s) 527, 540, 1703
AcoI YGGCCR 3 cut(s) 416, 597, 678
AcuI CTGAAG 9 cut(s) 308, 882, 1680, 1827, 1857, 2463, 2826, 3237, 3489
AfaI GTAC 7 cut(s) 146, 339, 1444, 1743, 2037, 2531, 2559
AfeI AGCGCT 2 cut(s) 3056, 3481
AfiI CCNNNNNNNGG 3 cut(s) 130, 425, 707
AflIII ACRYGT 3 cut(s) 172, 1911, 2455
AhdI GACNNNNNGTC 1 cut(s) 3355
AjiI CACGTC 1 cut(s) 1914
AjnI CCWGG 3 cut(s) 93, 937, 2052
AjuI GAANNNNNNNTTGG 2 cut(s) 2899, 2931
Alw21I GWGCWC 1 cut(s) 2414
Alw26I GTCTC 7 cut(s) 400, 1169, 1234, 2551, 2917, 3143, 3342
AlwI GGATC 3 cut(s) 527, 540, 1703
AlwNI CAGNNNCTG 2 cut(s) 1016, 1115
Ama87I CYCGRG 4 cut(s) 536, 1066, 2550, 3325
Aor51HI AGCGCT 2 cut(s) 3056, 3481
AoxI GGCC 8 cut(s) 120, 416, 475, 597, 678, 1879, 2235, 2257
AseI ATTAAT 1 cut(s) 1422
Asp700I GAANNNNTTC 4 cut(s) 1582, 2709, 2722, 2839
Asp718I GGTACC 1 cut(s) 1741
AspLEI GCGC 4 cut(s) 2159, 3038, 3057, 3482
AsuC2I CCSGG 1 cut(s) 2109
AsuHPI GGTGA 8 cut(s) 109, 190, 459, 804, 1191, 1541, 1752, 2510
AsuII TTCGAA 1 cut(s) 3404
AvaI CYCGRG 4 cut(s) 536, 1066, 2550, 3325
AvaII GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3135, 3436
AxyI CCTNAGG 1 cut(s) 561
BalI TGGCCA 2 cut(s) 599, 680
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 1741
BanII GRGCYC 1 cut(s) 2901
BbsI GAAGAC 4 cut(s) 1401, 1458, 1964, 2975
Bbv12I GWGCWC 1 cut(s) 2414
BbvCI CCTCAGC 1 cut(s) 2700
BccI CCATC 9 cut(s) 467, 767, 1369, 1569, 1805, 1871, 2128, 2311, 2634
BceAI ACGGC 2 cut(s) 38, 54
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 1047, 1081
BciT130I CCWGG 3 cut(s) 95, 939, 2054
BciVI GTATCC 1 cut(s) 2650
BclI TGATCA 3 cut(s) 724, 913, 1414
BcnI CCSGG 1 cut(s) 2109
BcoDI GTCTC 7 cut(s) 400, 1169, 1234, 2551, 2917, 3143, 3342
BfmI CTRYAG 5 cut(s) 399, 999, 1056, 1824, 2928
BfoI RGCGCY 4 cut(s) 2160, 3039, 3058, 3483
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 1 cut(s) 2650
BglII AGATCT 2 cut(s) 1328, 1435
Bme1390I CCNGG 4 cut(s) 95, 939, 2054, 2109
Bme18I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3135, 3436
BmeRI GACNNNNNGTC 1 cut(s) 3355
BmeT110I CYCGRG 4 cut(s) 536, 1066, 2550, 3325
BmgBI CACGTC 1 cut(s) 1914
BmiI GGNNCC 6 cut(s) 534, 645, 699, 1743, 1839, 3437
BmrFI CCNGG 4 cut(s) 95, 939, 2054, 2109
BmrI ACTGGG 2 cut(s) 2441, 2982
BmsI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1858, 2024, 2290, 2818
BmuI ACTGGG 2 cut(s) 2441, 2982
BoxI GACNNNNGTC 1 cut(s) 2981
BpiI GAAGAC 4 cut(s) 1401, 1458, 1964, 2975
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpmI CTGGAG 2 cut(s) 152, 1151
Bpu10I CCTNAGC 1 cut(s) 2700
Bpu14I TTCGAA 1 cut(s) 3404
BpuEI CTTGAG 5 cut(s) 726, 2322, 2887, 2986, 3563
BpuMI CCSGG 1 cut(s) 2109
Bsa29I ATCGAT 1 cut(s) 1180
BsaBI GATNNNNATC 1 cut(s) 1182
BsaI GGTCTC 3 cut(s) 2551, 2917, 3342
BsaWI WCCGGW 1 cut(s) 1247
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 425, 707
Bse1I ACTGG 6 cut(s) 600, 681, 1450, 2447, 2977, 3092
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 2317, 2733
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 3 cut(s) 95, 939, 2054
BseCI ATCGAT 1 cut(s) 1180
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 3 cut(s) 130, 425, 707
BseMI GCAATG 2 cut(s) 2317, 2733
BseNI ACTGG 6 cut(s) 600, 681, 1450, 2447, 2977, 3092
BseRI GAGGAG 5 cut(s) 1590, 1913, 1916, 2989, 3405
BseX3I CGGCCG 1 cut(s) 416
BsgI GTGCAG 2 cut(s) 1802, 3090
Bsh1236I CGCG 1 cut(s) 3142
Bsh1285I CGRYCG 2 cut(s) 419, 1387
BshFI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1881, 2237, 2259
BshNI GGYRCC 1 cut(s) 1741
BshVI ATCGAT 1 cut(s) 1180
BsiEI CGRYCG 2 cut(s) 419, 1387
BsiHKAI GWGCWC 1 cut(s) 2414
BsiHKCI CYCGRG 4 cut(s) 536, 1066, 2550, 3325
BsiSI CCGG 4 cut(s) 419, 1248, 2109, 2401
BslFI GGGAC 6 cut(s) 657, 1659, 1814, 2608, 2989, 3449
BslI CCNNNNNNNGG 3 cut(s) 130, 425, 707
BsmAI GTCTC 7 cut(s) 400, 1169, 1234, 2551, 2917, 3143, 3342
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 6 cut(s) 657, 1659, 1814, 2608, 2989, 3449
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1881, 2237, 2259
Bso31I GGTCTC 3 cut(s) 2551, 2917, 3342
BsoBI CYCGRG 4 cut(s) 536, 1066, 2550, 3325
Bsp119I TTCGAA 1 cut(s) 3404
Bsp1286I GDGCHC 2 cut(s) 2414, 2901
Bsp1407I TGTACA 1 cut(s) 2529
BspACI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
BspANI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1881, 2237, 2259
BspDI ATCGAT 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 3142
BspHI TCATGA 3 cut(s) 631, 1210, 1951
BspLI GGNNCC 6 cut(s) 534, 645, 699, 1743, 1839, 3437
BspMAI CTGCAG 3 cut(s) 403, 1003, 1060
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 3 cut(s) 527, 540, 1703
BspQI GCTCTTC 1 cut(s) 2382
BspT104I TTCGAA 1 cut(s) 3404
BspT107I GGYRCC 1 cut(s) 1741
BspTNI GGTCTC 3 cut(s) 2551, 2917, 3342
BsrDI GCAATG 2 cut(s) 2317, 2733
BsrGI TGTACA 1 cut(s) 2529
BsrI ACTGG 6 cut(s) 600, 681, 1450, 2447, 2977, 3092
BssT1I CCWWGG 5 cut(s) 1712, 2126, 2160, 2644, 3240
Bst2UI CCWGG 3 cut(s) 95, 939, 2054
Bst4CI ACNGT 9 cut(s) 1245, 1828, 1895, 1963, 2182, 2192, 2353, 2818, 3199
Bst6I CTCTTC 7 cut(s) 180, 543, 2382, 2782, 2866, 3256, 3505
BstAUI TGTACA 1 cut(s) 2529
BstBI TTCGAA 1 cut(s) 3404
BstC8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 1999
BstFNI CGCG 1 cut(s) 3142
BstH2I RGCGCY 4 cut(s) 2160, 3039, 3058, 3483
BstHHI GCGC 4 cut(s) 2159, 3038, 3057, 3482
BstMAI GTCTC 7 cut(s) 400, 1169, 1234, 2551, 2917, 3143, 3342
BstMCI CGRYCG 2 cut(s) 419, 1387
BstNI CCWGG 3 cut(s) 95, 939, 2054
BstNSI RCATGY 5 cut(s) 176, 442, 932, 1271, 2459
BstPAI GACNNNNGTC 1 cut(s) 2981
BstSCI CCNGG 4 cut(s) 93, 937, 2052, 2107
BstSFI CTRYAG 5 cut(s) 399, 999, 1056, 1824, 2928
BstUI CGCG 1 cut(s) 3142
BstV2I GAAGAC 4 cut(s) 1401, 1458, 1964, 2975
BstX2I RGATCY 4 cut(s) 532, 1328, 1435, 1708
BstYI RGATCY 4 cut(s) 532, 1328, 1435, 1708
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1180
Bsu36I CCTNAGG 1 cut(s) 561
BsuI GTATCC 1 cut(s) 2650
BsuRI GGCC 8 cut(s) 122, 418, 477, 599, 680, 1881, 2237, 2259
BsuTUI ATCGAT 1 cut(s) 1180
BtrI CACGTC 1 cut(s) 1914
BtsIMutI CAGTG 6 cut(s) 1785, 1900, 2187, 2197, 2880, 3513
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 8 cut(s) 24, 51, 362, 962, 1036, 1231, 1269, 1999
CaiI CAGNNNCTG 2 cut(s) 1016, 1115
CciI TCATGA 3 cut(s) 631, 1210, 1951
CfoI GCGC 4 cut(s) 2159, 3038, 3057, 3482
ClaI ATCGAT 1 cut(s) 1180
CpoI CGGWCCG 1 cut(s) 578
Csp6I GTAC 7 cut(s) 145, 338, 1443, 1742, 2036, 2530, 2558
CspCI CAANNNNNGTGG 2 cut(s) 2716, 2751
CspI CGGWCCG 1 cut(s) 578
CviQI GTAC 7 cut(s) 145, 338, 1443, 1742, 2036, 2530, 2558
DrdI GACNNNNNNGTC 1 cut(s) 1243
DriI GACNNNNNGTC 1 cut(s) 3355
DseDI GACNNNNNNGTC 1 cut(s) 1243
EaeI YGGCCR 3 cut(s) 416, 597, 678
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 7 cut(s) 180, 543, 2382, 2782, 2866, 3256, 3505
Eam1105I GACNNNNNGTC 1 cut(s) 3355
EarI CTCTTC 7 cut(s) 180, 543, 2382, 2782, 2866, 3256, 3505
EciI GGCGGA 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 5 cut(s) 1712, 2126, 2160, 2644, 3240
Eco24I GRGCYC 1 cut(s) 2901
Eco31I GGTCTC 3 cut(s) 2551, 2917, 3342
Eco32I GATATC 2 cut(s) 355, 1184
Eco47I GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3135, 3436
Eco47III AGCGCT 2 cut(s) 3056, 3481
Eco52I CGGCCG 1 cut(s) 416
Eco57I CTGAAG 9 cut(s) 308, 882, 1680, 1827, 1857, 2463, 2826, 3237, 3489
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 4 cut(s) 536, 1066, 2550, 3325
EcoO109I RGGNCCY 3 cut(s) 565, 644, 3135
EcoRI GAATTC 2 cut(s) 784, 1582
EcoRII CCWGG 3 cut(s) 93, 937, 2052
EcoRV GATATC 2 cut(s) 355, 1184
EcoT14I CCWWGG 5 cut(s) 1712, 2126, 2160, 2644, 3240
EcoT22I ATGCAT 2 cut(s) 930, 1269
EcoT38I GRGCYC 1 cut(s) 2901
ErhI CCWWGG 5 cut(s) 1712, 2126, 2160, 2644, 3240
Esp3I CGTCTC 1 cut(s) 400
FalI AAGNNNNNCTT 4 cut(s) 2244, 2276, 2995, 3027
FaqI GGGAC 6 cut(s) 657, 1659, 1814, 2608, 2989, 3449
FauI CCCGC 1 cut(s) 31
FbaI TGATCA 3 cut(s) 724, 913, 1414
FblI GTMKAC 1 cut(s) 3088
FriOI GRGCYC 1 cut(s) 2901
GlaI GCGC 4 cut(s) 2158, 3037, 3056, 3481
GsuI CTGGAG 2 cut(s) 152, 1151
HaeII RGCGCY 4 cut(s) 2160, 3039, 3058, 3483
HaeIII GGCC 8 cut(s) 122, 418, 477, 599, 680, 1881, 2237, 2259
HapII CCGG 4 cut(s) 419, 1248, 2109, 2401
HhaI GCGC 4 cut(s) 2159, 3038, 3057, 3482
Hin6I GCGC 4 cut(s) 2157, 3036, 3055, 3480
HinP1I GCGC 4 cut(s) 2157, 3036, 3055, 3480
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 2 cut(s) 47, 3536
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 4 cut(s) 419, 1248, 2109, 2401
HphI GGTGA 8 cut(s) 109, 190, 459, 804, 1191, 1541, 1752, 2510
Hpy166II GTNNAC 7 cut(s) 686, 713, 1555, 2117, 3089, 3529, 3570
Hpy8I GTNNAC 7 cut(s) 686, 713, 1555, 2117, 3089, 3529, 3570
HpyCH4III ACNGT 9 cut(s) 1245, 1828, 1895, 1963, 2182, 2192, 2353, 2818, 3199
HpyCH4IV ACGT 7 cut(s) 587, 1207, 1398, 1768, 1913, 2663, 3064
HpySE526I ACGT 7 cut(s) 587, 1207, 1398, 1768, 1913, 2663, 3064
HspAI GCGC 4 cut(s) 2157, 3036, 3055, 3480
KpnI GGTACC 1 cut(s) 1745
Ksp22I TGATCA 3 cut(s) 724, 913, 1414
KspAI GTTAAC 1 cut(s) 1555
LguI GCTCTTC 1 cut(s) 2382
LmnI GCTCC 5 cut(s) 12, 619, 1603, 1716, 2409
LweI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1858, 2024, 2290, 2818
MaeII ACGT 7 cut(s) 587, 1207, 1398, 1768, 1913, 2663, 3064
MaeIII GTNAC 6 cut(s) 1197, 1496, 1858, 2449, 2458, 2516
MfeI CAATTG 4 cut(s) 125, 279, 504, 1572
MflI RGATCY 4 cut(s) 532, 1328, 1435, 1708
MhlI GDGCHC 2 cut(s) 2414, 2901
MlsI TGGCCA 2 cut(s) 599, 680
MluNI TGGCCA 2 cut(s) 599, 680
MlyI GAGTC 1 cut(s) 3539
MmeI TCCRAC 2 cut(s) 1887, 2372
Mox20I TGGCCA 2 cut(s) 599, 680
Mph1103I ATGCAT 2 cut(s) 930, 1269
MroXI GAANNNNTTC 4 cut(s) 1582, 2709, 2722, 2839
MscI TGGCCA 2 cut(s) 599, 680
MseI TTAA 6 cut(s) 443, 984, 1422, 1554, 2679, 3333
MslI CAYNNNNRTG 1 cut(s) 1916
Msp20I TGGCCA 2 cut(s) 599, 680
MspA1I CMGCKG 3 cut(s) 404, 1013, 1055
MspI CCGG 4 cut(s) 419, 1248, 2109, 2401
MspR9I CCNGG 4 cut(s) 95, 939, 2054, 2109
MunI CAATTG 4 cut(s) 125, 279, 504, 1572
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 3 cut(s) 95, 939, 2054
MvnI CGCG 1 cut(s) 3142
NciI CCSGG 1 cut(s) 2109
NlaIV GGNNCC 6 cut(s) 534, 645, 699, 1743, 1839, 3437
NmuCI GTSAC 4 cut(s) 1197, 2449, 2458, 2516
NsiI ATGCAT 2 cut(s) 930, 1269
NspI RCATGY 5 cut(s) 176, 442, 932, 1271, 2459
NspV TTCGAA 1 cut(s) 3404
PaeI GCATGC 1 cut(s) 1271
PaeR7I CTCGAG 2 cut(s) 1066, 2550
PagI TCATGA 3 cut(s) 631, 1210, 1951
PasI CCCWGGG 1 cut(s) 2053
PciI ACATGT 2 cut(s) 172, 2455
PciSI GCTCTTC 1 cut(s) 2382
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 4 cut(s) 1582, 2709, 2722, 2839
PflMI CCANNNNNTGG 1 cut(s) 130
Ple19I CGATCG 1 cut(s) 1387
PleI GAGTC 1 cut(s) 3539
PpsI GAGTC 1 cut(s) 3539
PpuMI RGGWCCY 3 cut(s) 565, 644, 3135
PscI ACATGT 2 cut(s) 172, 2455
PshAI GACNNNNGTC 1 cut(s) 2981
PshBI ATTAAT 1 cut(s) 1422
Psp1406I AACGTT 1 cut(s) 3064
Psp5II RGGWCCY 3 cut(s) 565, 644, 3135
Psp6I CCWGG 3 cut(s) 93, 937, 2052
PspGI CCWGG 3 cut(s) 93, 937, 2052
PspN4I GGNNCC 6 cut(s) 534, 645, 699, 1743, 1839, 3437
PspPPI RGGWCCY 3 cut(s) 565, 644, 3135
PsrI GAACNNNNNNTAC 2 cut(s) 3205, 3237
PstI CTGCAG 3 cut(s) 403, 1003, 1060
PstNI CAGNNNCTG 2 cut(s) 1016, 1115
PsuI RGATCY 4 cut(s) 532, 1328, 1435, 1708
PvuI CGATCG 1 cut(s) 1387
PvuII CAGCTG 3 cut(s) 404, 1013, 1055
RsaI GTAC 7 cut(s) 146, 339, 1444, 1743, 2037, 2531, 2559
RsaNI GTAC 7 cut(s) 145, 338, 1443, 1742, 2036, 2530, 2558
RseI CAYNNNNRTG 1 cut(s) 1916
Rsr2I CGGWCCG 1 cut(s) 578
RsrII CGGWCCG 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 2382
SaqAI TTAA 6 cut(s) 443, 984, 1422, 1554, 2679, 3333
SchI GAGTC 1 cut(s) 3539
ScrFI CCNGG 4 cut(s) 95, 939, 2054, 2109
SduI GDGCHC 2 cut(s) 2414, 2901
SfaNI GCATC 9 cut(s) 79, 326, 979, 1004, 1269, 1858, 2024, 2290, 2818
SfcI CTRYAG 5 cut(s) 399, 999, 1056, 1824, 2928
Sfr274I CTCGAG 2 cut(s) 1066, 2550
SfuI TTCGAA 1 cut(s) 3404
SinI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3135, 3436
SlaI CTCGAG 2 cut(s) 1066, 2550
SmiMI CAYNNNNRTG 1 cut(s) 1916
SmlI CTYRAG 7 cut(s) 741, 1066, 2337, 2550, 2902, 3001, 3578
SmoI CTYRAG 7 cut(s) 741, 1066, 2337, 2550, 2902, 3001, 3578
SphI GCATGC 1 cut(s) 1271
SsiI CCGC 6 cut(s) 24, 332, 358, 415, 436, 665
SspI AATATT 2 cut(s) 1076, 1306
StyD4I CCNGG 4 cut(s) 93, 937, 2052, 2107
StyI CCWWGG 5 cut(s) 1712, 2126, 2160, 2644, 3240
TaaI ACNGT 9 cut(s) 1245, 1828, 1895, 1963, 2182, 2192, 2353, 2818, 3199
TaiI ACGT 7 cut(s) 590, 1210, 1401, 1771, 1916, 2666, 3067
TaqI TCGA 7 cut(s) 59, 216, 1067, 1180, 2551, 3404, 3589
TaqII GACCGA 2 cut(s) 595, 3065
TatI WGTACW 3 cut(s) 144, 2529, 2557
TauI GCSGC 2 cut(s) 418, 438
Tru1I TTAA 6 cut(s) 443, 984, 1422, 1554, 2679, 3333
Tru9I TTAA 6 cut(s) 443, 984, 1422, 1554, 2679, 3333
TscAI CASTG 6 cut(s) 1785, 1900, 2187, 2197, 2887, 3513
TseFI GTSAC 4 cut(s) 1197, 2449, 2458, 2516
Tsp45I GTSAC 4 cut(s) 1197, 2449, 2458, 2516
TspGWI ACGGA 2 cut(s) 591, 1744
TspRI CASTG 6 cut(s) 1785, 1900, 2187, 2197, 2887, 3513
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 7 cut(s) 565, 578, 644, 710, 776, 3135, 3436
VspI ATTAAT 1 cut(s) 1422
XbaI TCTAGA 1 cut(s) 2759
XceI RCATGY 5 cut(s) 176, 442, 932, 1271, 2459
XhoI CTCGAG 2 cut(s) 1066, 2550
XmiI GTMKAC 1 cut(s) 3088
XmnI GAANNNNTTC 4 cut(s) 1582, 2709, 2722, 2839
Zsp2I ATGCAT 2 cut(s) 930, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.