MD02G1089000.v1.1

Domain found in a variety of signalling proteins, always encircled by uDENN and dDENN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003032
Physical Location & Seq
Reverse (-)
6999303 .. 7002957
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1089000.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAATAAAGATGAAGAGACGGGTAGCCCCAGTTGGGGTGCTTCGTTTTTCGCACAAACGGAAGATGTTGCAAGAGCAGTTGCTGCTGTAGCGGCTGCTGCTACTGCCACTCATTCTCCGCGGCCATCTGCTGTGTACTCATTGAATGAAGATGGTGGTGGTGGCAGTCCGCTTCAGAGGTTACAACGCCATGTTACGAAAGTTCTAAAAGGTTTCCAGCATCCTCCTGAAGTGAAGCGAGGAACTTACAATCCTGAAGTTTTGACTAGCCAGAAGCGGCAATGGGCAAACTTTCAGTTGCAGTATTTGGATCATAAGTCTTTAAAGCAGCCAACAAGGCTTTTTGAGAGCATGGTAGTTGTGGGGCTTCATCCTAACTGTGATATTCAGGCACTTCAAGGGCAATACATTGGAAGAAAGTCTGAAGGTTCAGGGAGATTTAGTAGTCAGAATCACTCTCGTATAGAACCTAATATCGAACCGCAGGTGTTATTTGTTTATCCTCCGGAAAAGCAGCTGCCTCTACAATACAAGGATCTGCTTTCATTTTGCTTTCCCAGAGGCGTAGAGGTTCATGCTGTTGAGAGAACTCCTTCAATGAGTGAGTTGAATGAAATTCTTCTGGGGCAGGAGCATTTTAAACGGAGCGATCTGTCATTTGTATTCAGACTACAGGTTGCCGATGATTCAACTTTGTATGGGTGCTGTGTGTTAGTGGAAGAAGTAGTGCAAAAACCCTCTGGATTGCTTTCCATGATTGCAGAGAAACATCCTTCTCGCCCGTCTTTGAGCCGTCATATATTAACCACTCAGAGATGTTATTGCATTCTCTCAAGGGTTCCAGCATTTGAGTTACATTTTGGTGTATTGAATAGCATCTTCACAGAGGAAAGGTTGGAGCGATTAACCCAAGGAATAGATCTTTTAGATTTAGAAAGCCCAAAGGATTGTGACAATGGTGAAATTTTCGAAGATATACTACAGAAGAAACTTCAAATGGTGTATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DENN uDENN

Protein Analysis

337

Amino Acids

37.89

Weight (kDa)

6.32

Isoelectric Point (pI)

59.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
uDENN PF03456 161 - 238 3.1e-08 uDENN domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
Acc36I ACCTGC 1 cut(s) 475
AccII CGCG 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 6 cut(s) 92, 119, 121, 170, 277, 482
AclWI GGATC 2 cut(s) 318, 543
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 2 cut(s) 615, 963
AcuI CTGAAG 4 cut(s) 158, 249, 276, 444
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 34, 35
AgsI TTSAA 7 cut(s) 145, 398, 597, 610, 690, 871, 995
AloI GAACNNNNNNTCC 2 cut(s) 235, 267
AluBI AGCT 1 cut(s) 517
AluI AGCT 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 2 cut(s) 318, 543
AlwNI CAGNNNCTG 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 6 cut(s) 83, 95, 98, 328, 514, 517
ApoI RAATTY 2 cut(s) 615, 963
Asp700I GAANNNNTTC 2 cut(s) 592, 618
AsuHPI GGTGA 1 cut(s) 971
AsuII TTCGAA 1 cut(s) 969
BarI GAAGNNNNNNTAC 2 cut(s) 963, 995
BbvI GCAGC 6 cut(s) 70, 82, 85, 340, 504, 526
BccI CCATC 2 cut(s) 133, 146
BceAI ACGGC 1 cut(s) 777
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 3 cut(s) 87, 671, 980
BfuAI ACCTGC 1 cut(s) 475
BglI GCCNNNNNGGC 1 cut(s) 337
BglII AGATCT 1 cut(s) 919
BisI GCNGC 9 cut(s) 84, 93, 96, 99, 122, 278, 329, 515, 518
BlsI GCNGC 9 cut(s) 85, 94, 97, 100, 123, 279, 330, 516, 519
BmiI GGNNCC 1 cut(s) 840
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 229, 885
BmuI ACTGGG 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 817
BsaJI CCNNGG 2 cut(s) 119, 910
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 4 cut(s) 100, 130, 427, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 35
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 287
BseAI TCCGGA 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 119, 910
BseGI GGATG 3 cut(s) 220, 370, 769
BseLI CCNNNNNNNGG 2 cut(s) 34, 35
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 824
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 6 cut(s) 70, 82, 85, 340, 504, 526
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 124
BsiSI CCGG 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 34, 35
BsmAI GTCTC 1 cut(s) 11
BsmBI CGTCTC 1 cut(s) 11
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 1 cut(s) 124
Bsp119I TTCGAA 1 cut(s) 969
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 4 cut(s) 310, 535, 649, 919
BspACI CCGC 6 cut(s) 92, 119, 121, 170, 277, 482
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 1 cut(s) 823
BspEI TCCGGA 1 cut(s) 505
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 840
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 2 cut(s) 318, 543
BspT104I TTCGAA 1 cut(s) 969
BsrDI GCAATG 1 cut(s) 287
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 119, 910
BssMI GATC 4 cut(s) 310, 535, 649, 919
BssT1I CCWWGG 1 cut(s) 910
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 969
BstDEI CTNAG 1 cut(s) 810
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 220, 370, 769
BstFNI CGCG 1 cut(s) 121
BstKTI GATC 4 cut(s) 313, 538, 652, 922
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 4 cut(s) 310, 535, 649, 919
BstMWI GCNNNNNNNGC 6 cut(s) 83, 89, 92, 98, 104, 337
BstSFI CTRYAG 3 cut(s) 87, 671, 980
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 6 cut(s) 70, 82, 85, 340, 504, 526
BstX2I RGATCY 2 cut(s) 535, 919
BstYI RGATCY 2 cut(s) 535, 919
BsuRI GGCC 1 cut(s) 124
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 3 cut(s) 220, 370, 769
BveI ACCTGC 1 cut(s) 475
CaiI CAGNNNCTG 1 cut(s) 83
Cfr42I CCGCGG 1 cut(s) 122
Csp6I GTAC 1 cut(s) 136
CviAII CATG 4 cut(s) 191, 352, 575, 754
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 810
DpnI GATC 4 cut(s) 312, 537, 651, 921
DpnII GATC 4 cut(s) 310, 535, 649, 919
DraI TTTAAA 2 cut(s) 324, 640
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 910
Eco57I CTGAAG 4 cut(s) 158, 249, 276, 444
EcoT14I CCWWGG 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 910
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 4 cut(s) 194, 355, 578, 757
FatI CATG 4 cut(s) 190, 351, 574, 753
Fnu4HI GCNGC 9 cut(s) 84, 93, 96, 99, 122, 278, 329, 515, 518
FokI GGATG 3 cut(s) 207, 357, 756
Fsp4HI GCNGC 9 cut(s) 84, 93, 96, 99, 122, 278, 329, 515, 518
FspBI CTAG 1 cut(s) 267
GluI GCNGC 9 cut(s) 84, 93, 96, 99, 122, 278, 329, 515, 518
HaeIII GGCC 1 cut(s) 124
HapII CCGG 1 cut(s) 506
Hin1II CATG 4 cut(s) 194, 355, 578, 757
HinfI GANTC 2 cut(s) 451, 686
HpaII CCGG 1 cut(s) 506
HphI GGTGA 1 cut(s) 971
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 5 cut(s) 177, 424, 450, 668, 813
Hpy188III TCNNGA 4 cut(s) 227, 254, 506, 741
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 3 cut(s) 419, 603, 783
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 71, 301, 730, 761, 825
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 89, 92, 98, 104, 337
HpyF3I CTNAG 1 cut(s) 810
Hsp92II CATG 4 cut(s) 194, 355, 578, 757
Kpn2I TCCGGA 1 cut(s) 505
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 4 cut(s) 310, 535, 649, 919
LmnI GCTCC 3 cut(s) 631, 645, 898
Lsp1109I GCAGC 6 cut(s) 70, 82, 85, 340, 504, 526
LweI GCATC 2 cut(s) 229, 885
MaeI CTAG 1 cut(s) 267
MaeIII GTNAC 4 cut(s) 180, 193, 852, 950
MalI GATC 4 cut(s) 312, 537, 651, 921
MboI GATC 4 cut(s) 310, 535, 649, 919
MboII GAAGA 9 cut(s) 26, 74, 161, 426, 611, 731, 871, 983, 997
MflI RGATCY 2 cut(s) 535, 919
MluCI AATT 2 cut(s) 615, 963
MmeI TCCRAC 1 cut(s) 876
MnlI CCTC 9 cut(s) 171, 233, 234, 513, 531, 554, 562, 748, 880
MroI TCCGGA 1 cut(s) 505
MroXI GAANNNNTTC 2 cut(s) 592, 618
MseI TTAA 4 cut(s) 323, 639, 803, 905
MslI CAYNNNNRTG 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 121, 517
MspI CCGG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 825
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 6 cut(s) 83, 89, 92, 98, 104, 337
NdeII GATC 4 cut(s) 310, 535, 649, 919
NlaIII CATG 4 cut(s) 194, 355, 578, 757
NlaIV GGNNCC 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 950
NspV TTCGAA 1 cut(s) 969
PaqCI CACCTGC 1 cut(s) 475
PctI GAATGC 1 cut(s) 825
PdmI GAANNNNTTC 2 cut(s) 592, 618
PfeI GAWTC 2 cut(s) 451, 686
PkrI GCNGC 9 cut(s) 85, 94, 97, 100, 123, 279, 330, 516, 519
PspN4I GGNNCC 1 cut(s) 840
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 535, 919
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 861
SacII CCGCGG 1 cut(s) 122
SaqAI TTAA 4 cut(s) 323, 639, 803, 905
SatI GCNGC 9 cut(s) 84, 93, 96, 99, 122, 278, 329, 515, 518
Sau3AI GATC 4 cut(s) 310, 535, 649, 919
SetI ASST 9 cut(s) 182, 214, 430, 472, 489, 519, 573, 678, 896
SfaNI GCATC 2 cut(s) 229, 885
SfcI CTRYAG 3 cut(s) 87, 671, 980
Sfr303I CCGCGG 1 cut(s) 122
SfuI TTCGAA 1 cut(s) 969
SgrBI CCGCGG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
Sse9I AATT 2 cut(s) 615, 963
SsiI CCGC 6 cut(s) 92, 119, 121, 170, 277, 482
SspMI CTAG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 477, 969
TasI AATT 2 cut(s) 615, 963
TatI WGTACW 1 cut(s) 135
TauI GCSGC 3 cut(s) 95, 124, 280
TfiI GAWTC 2 cut(s) 451, 686
Tru1I TTAA 4 cut(s) 323, 639, 803, 905
Tru9I TTAA 4 cut(s) 323, 639, 803, 905
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 6 cut(s) 83, 95, 98, 328, 514, 517
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 7 cut(s) 17, 27, 162, 359, 534, 563, 627
TspGWI ACGGA 2 cut(s) 74, 658
XapI RAATTY 2 cut(s) 615, 963
XmnI GAANNNNTTC 2 cut(s) 592, 618
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.