Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003032

Physical Location & Seq

Genomic Coordinates Reverse (-)

17437779 .. 17445511

Gene Structure 7733 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1216100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGTCAATGTTGAATTTTTTGATAGGAGGGAAGCCCCTTAGTATGAATAAAGATGAAGAGACGAGTAGCCCCAGTTGGGGGGCCTCGTTTTTTGCGCAAACGGAAGATGTTGCAAGAGCAGTTGCTGCTGTAGCGGCCTCTGCTACTGCAGCTCATTCTCCGCGGCCATCTGCTGTATACTCGTCGAACGAAGATGGTGGTGGTGGCAGTGGCAGTCCACTTCAGAGGTTACAACGCCATGTTACAAAGGTGCTAAAAGGTTTCCAACATCCTCCTGAAGTGAAGCGAGGAACTTACAATCCAGAAGTTTTGACTAGCCAGAAGCGGCAATGGGCATTGCAGTATTTGGATCATAAGTCTTTAAAACAGCCAACAAGGCTTTTTGAGAGCATGGTAGTTATGGGGCTTCATCCTAATTGTGATATTCAGGCACTTCAAAGGCAATACATTGGAAGAAAGTGTGATGGTCCAGGAAGATTTAATAGTCAGAATCGCTCTCGTGTAGAACCCAATATCGAACCTCAGGTGTTATTTGTTTATCCTCCGGAAAAGCAGCTGCCTCTACAATACAAGGATCTCCTTTCATTTTGCTTTCCTGGAGGTGTAGAGGTTCATGCTGTTGAGAGAACTCCTTCAATGAGTGAGTTAAATGAAATTCTTCTGGGACAGGAGCATTTTAAAAGGAGCGATCTGTCCTTTGTATTCAGATTACAGGTTGCTGATGATTCAACTTTGTATGGATGCTGCATGTTAGTCGAAGAAGTAGTGCAAAAACCCTCTGGATTGCTTTCCATGATTGCAGAGAAACATCCTTCTCGCCCATCTTTGAGCCGTCATATATTAACCACTCATAGATGTTATTGCATTCTCTCAAGGGTTCCAGCATTTGAGTTACATTTTGGTGTATTGAACAGCATCTTCACAGAGGAAAGGTTGGAACGATTAACACAAGGAATAGATCTTTTAGATTTAGAAAGCCCAAAGGATTATGACAGTGGTGAAAATTTTGAAGATACTACTGAAGAAACTTCAGATGGTGTATCACTAAGCAGTAGAACTGAGGAGGACATGCGGAATGGAACTGCAGAGTTTTCTCAATCGGGGAGATTTGCAGAAAATGGGAGTCACATAGATCATCAAATGGTAGATGGGAATTTTAACTTGTCAACGAGAAGGGTTAGTGGAAATGTTGTTGTTCCAGTTGAACCTGAAACTGAGATAGTTAGTTGCAAAGGAGAACCTGGCGGTGTGAATGCTGAAGTTTGTGAGGTCTACGTTGATGACTTCTCTACAAACAAACAAGCTGTAGAAAGGCGATTACCGAATGCTGTCCTACCTCTGCTGCGCTTCTATCAGTATGAAAGCTCTGATTCTTCCTGCAGTTTTCAAGGCTCTCCTAGTGAGGACAGAAATTTTAGGAGCGATGTTGATTTTACAGAGACGGACGAGGCATCTTTCTCTGGCCAAGATGATTCTGACCTCATTGATATTCTTGAATGGGCCAAGGCAAACAATCATGGATCATTGCAGATAATAAGTGAGTACTACCAATTATGTTGCCCTGCTAGAGGTACCACTGTTAAGTTTCATCCTTTGGACCACCTGCATCCCTTGGAATACCATAGACCAAAAGTAAACATTTTACGTACTGCGGGCTCAACCATTGATCTGAGGTCGTGCAATACGAGTCTGGAATTCGCTGAGGCACAAAGTGCTTTCTTGGTAGAGGAGGAAGCAACTGCATTATCAGTATGGGCTATTGCATGCATATGTGGCTCCTTACGACTTGAAAATGTATTAACATTGTTTGCAGGAGCACTGTTGGAGAAGCAGATTGTAATCATTTGTTCCAATTTGGGTATCTTGTCGGCCTCAGTGTTATCAATTATCCCTCTGATCCGCCCCTACCAGTGGCAAAGCCTTCTAATGCCGGTTTTACCAAATGACATGCTGGACTTTTTAGACGCACCTGTCCCTTACATAGTTGGTGTGAAGAACAAATATGAAGTACAGTCGAAACTATCCAATACAATTGTAGTTGACGCCAACAAGAACCAGGTGAAGTCGCCTACAATACCTCAATTACCACAACATAAGGAATTATTTTCAGCCTTAAGTCCTTATCATGCAAAGCTTGTGGGAGAGAGTTTTTTGGGTAGGAAAAGGCCAGTGTACGAATGCACAGAAGAACAGATTGTAGCTGCCAAGGGTTTCCTAGGAGTGTTGAGAACTTATTTGGATTCTCTTTGCTCGAACCTCCGTTCACATACAATTACTAACGTACAGTCCAATGATGATAAGGTATCATTACTTCTGAAGGAGAGTTTCATCGACTCATTCCCTAGTCGTGATCGACCATTTATGAAGCTTTTTGTGGACACACAAATGTTTTCTGTACATACGGATCTCGTTCTCTCTTTCTTCCAGAAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN uDENN

Protein Analysis

812

Amino Acids

90.61

Weight (kDa)

5.38

Isoelectric Point (pI)

55.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	174 - 251	3.2e-07	uDENN domain
DENN	PF02141	587 - 683	1.6e-22	DENN (AEX-3) domain
dDENN	PF03455	773 - 798	7.7e-06	dDENN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35560 AT5G35560
fragaria_vesca	FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450 FvH4_1g08450
malus_domestica	MD00G1142500.v1.1 MD02G1088900.v1.1 MD02G1089000.v1.1 MD15G1216100.v1.1
prunus_persica	Prupe.7G200900_v2.0.a1 Prupe.7G200900_v2.0.a1 Prupe.7G200900_v2.0.a1
pyrus_communis	pycom15g19170
rosa_chinensis	RchiOBHm_Chr2g0094551
rosa_laevigata	RLG00000016501
rosa_multiflora	Rmu_sc0004359.1_g000040
rosa_roxburghii	Rroxscaffold_2G00147070
rosa_rugosa	Rorug02G0044800 Rorug02G0044900 Rorug02G0045000 Rorug02G0045100 Rorug02G0045100 Rorug02G0045100
rosa_samantha	Rh2AG093000 Rh2BG093300 Rh2CG095700 Rh2DG092100
rosa_wichuraiana	Rw7G039010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1611
AasI	GACNNNNNNGTC	1 cut(s)	1970
Acc16I	TGCGCA	1 cut(s)	96
Acc36I	ACCTGC	1 cut(s)	1611
Acc65I	GGTACC	1 cut(s)	1571
AccB1I	GGYRCC	1 cut(s)	1571
AccI	GTMKAC	2 cut(s)	177, 1272
AccII	CGCG	1 cut(s)	163
AccIII	TCCGGA	1 cut(s)	544
AciI	CCGC	8 cut(s)	134, 161, 163, 325, 1072, 1245, 1652, 1900
AclWI	GGATC	5 cut(s)	357, 582, 1528, 1891, 2412
AcoI	YGGCCR	2 cut(s)	164, 1462
AcsI	RAATTY	6 cut(s)	13, 654, 1003, 1153, 1411, 1694
AcuI	CTGAAG	6 cut(s)	206, 297, 1014, 1041, 1278, 2336
AcyI	GRCGYC	1 cut(s)	2043
AdeI	CACNNNGTG	1 cut(s)	1712
AfaI	GTAC	7 cut(s)	1544, 1573, 1648, 2010, 2174, 2283, 2397
AfiI	CCNNNNNNNGG	5 cut(s)	76, 77, 78, 1568, 1911
AflII	CTTAAG	1 cut(s)	2113
AjnI	CCWGG	4 cut(s)	469, 595, 1240, 2055
AloI	GAACNNNNNNTCC	2 cut(s)	283, 315
AluBI	AGCT	7 cut(s)	152, 556, 1304, 1365, 2134, 2201, 2368
AluI	AGCT	7 cut(s)	152, 556, 1304, 1365, 2134, 2201, 2368
Alw21I	GWGCWC	1 cut(s)	1819
Alw26I	GTCTC	2 cut(s)	53, 1433
AlwI	GGATC	5 cut(s)	357, 582, 1528, 1891, 2412
AlwNI	CAGNNNCTG	1 cut(s)	125
Aor13HI	TCCGGA	1 cut(s)	544
AoxI	GGCC	7 cut(s)	81, 135, 164, 1462, 1500, 1869, 2165
ApeKI	GCWGC	7 cut(s)	125, 149, 553, 556, 744, 1342, 2201
ApoI	RAATTY	6 cut(s)	13, 654, 1003, 1153, 1411, 1694
Asp700I	GAANNNNTTC	2 cut(s)	631, 657
Asp718I	GGTACC	1 cut(s)	1571
AspA2I	CCTAGG	1 cut(s)	2215
AspLEI	GCGC	2 cut(s)	97, 1347
AspS9I	GGNCC	4 cut(s)	81, 467, 1500, 1597
AsuHPI	GGTGA	2 cut(s)	1010, 2071
AvaII	GGWCC	2 cut(s)	467, 1597
AvrII	CCTAGG	1 cut(s)	2215
AxyI	CCTNAGG	1 cut(s)	522
BaeI	ACNNNNGTAYC	2 cut(s)	1563, 1596
BalI	TGGCCA	1 cut(s)	1464
BanI	GGYRCC	1 cut(s)	1571
BanII	GRGCYC	1 cut(s)	1658
BarI	GAAGNNNNNNTAC	2 cut(s)	2295, 2327
BauI	CACGAG	1 cut(s)	498
Bbv12I	GWGCWC	1 cut(s)	1819
BbvCI	CCTCAGC	1 cut(s)	1701
BbvI	GCAGC	7 cut(s)	112, 161, 543, 565, 731, 1329, 2188
BccI	CCATC	6 cut(s)	175, 188, 458, 829, 1028, 1142
BceAI	ACGGC	1 cut(s)	816
BcgI	CGANNNNNNTGC	2 cut(s)	736, 770
BciT130I	CCWGG	4 cut(s)	471, 597, 1242, 2057
BcoDI	GTCTC	2 cut(s)	53, 1433
BfaI	CTAG	5 cut(s)	315, 1398, 1566, 2216, 2343
BfmI	CTRYAG	5 cut(s)	129, 147, 1083, 1305, 1378
BfrI	CTTAAG	1 cut(s)	2113
BfuAI	ACCTGC	1 cut(s)	1611
BglI	GCCNNNNNGGC	1 cut(s)	376
BglII	AGATCT	1 cut(s)	958
BlnI	CCTAGG	1 cut(s)	2215
BmcAI	AGTACT	1 cut(s)	1544
Bme1390I	CCNGG	4 cut(s)	471, 597, 1242, 2057
Bme18I	GGWCC	2 cut(s)	467, 1597
BmgT120I	GGNCC	4 cut(s)	81, 467, 1500, 1597
BmiI	GGNNCC	4 cut(s)	82, 879, 1573, 1777
BmrFI	CCNGG	4 cut(s)	471, 597, 1242, 2057
BmrI	ACTGGG	1 cut(s)	66
BmsI	GCATC	4 cut(s)	731, 924, 1460, 1615
BmuI	ACTGGG	1 cut(s)	66
BpmI	CTGGAG	1 cut(s)	618
Bpu10I	CCTNAGC	1 cut(s)	1701
BpuEI	CTTGAG	1 cut(s)	856
BsaAI	YACGTR	1 cut(s)	1646
BsaBI	GATNNNNATC	1 cut(s)	1838
BsaHI	GRCGYC	1 cut(s)	2043
BsaJI	CCNNGG	5 cut(s)	161, 1503, 1611, 2205, 2215
BsaWI	WCCGGW	1 cut(s)	544
Bsc4I	CCNNNNNNNGG	5 cut(s)	76, 77, 78, 1568, 1911
Bse118I	RCCGGY	1 cut(s)	1930
Bse1I	ACTGG	4 cut(s)	72, 1199, 1909, 2168
Bse21I	CCTNAGG	1 cut(s)	522
Bse3DI	GCAATG	3 cut(s)	335, 335, 1523
Bse8I	GATNNNNATC	1 cut(s)	1838
BseAI	TCCGGA	1 cut(s)	544
BseBI	CCWGG	4 cut(s)	471, 597, 1242, 2057
BseDI	CCNNGG	5 cut(s)	161, 1503, 1611, 2205, 2215
BseGI	GGATG	6 cut(s)	268, 409, 746, 808, 1588, 1606
BseJI	GATNNNNATC	1 cut(s)	1838
BseLI	CCNNNNNNNGG	5 cut(s)	76, 77, 78, 1568, 1911
BseMI	GCAATG	3 cut(s)	335, 335, 1523
BseMII	CTCAG	6 cut(s)	536, 1050, 1206, 1661, 1692, 1887
BseNI	ACTGG	4 cut(s)	72, 1199, 1909, 2168
BseRI	GAGGAG	2 cut(s)	1076, 1742
BseXI	GCAGC	7 cut(s)	112, 161, 543, 565, 731, 1329, 2188
Bsh1236I	CGCG	1 cut(s)	163
BshFI	GGCC	7 cut(s)	83, 137, 166, 1464, 1502, 1871, 2167
BshNI	GGYRCC	1 cut(s)	1571
BsiHKAI	GWGCWC	1 cut(s)	1819
BsiSI	CCGG	2 cut(s)	545, 1931
BslFI	GGGAC	2 cut(s)	678, 1958
BslI	CCNNNNNNNGG	5 cut(s)	76, 77, 78, 1568, 1911
BsmAI	GTCTC	2 cut(s)	53, 1433
BsmBI	CGTCTC	2 cut(s)	53, 1433
BsmFI	GGGAC	2 cut(s)	678, 1958
BsmI	GAATGC	4 cut(s)	864, 1258, 1330, 2183
BsnI	GGCC	7 cut(s)	83, 137, 166, 1464, 1502, 1871, 2167
Bsp1286I	GDGCHC	2 cut(s)	1658, 1819
Bsp13I	TCCGGA	1 cut(s)	544
Bsp1407I	TGTACA	1 cut(s)	2395
BspACI	CCGC	8 cut(s)	134, 161, 163, 325, 1072, 1245, 1652, 1900
BspANI	GGCC	7 cut(s)	83, 137, 166, 1464, 1502, 1871, 2167
BspCNI	CTCAG	6 cut(s)	535, 1051, 1207, 1662, 1693, 1886
BspEI	TCCGGA	1 cut(s)	544
BspFNI	CGCG	1 cut(s)	163
BspLI	GGNNCC	4 cut(s)	82, 879, 1573, 1777
BspMAI	CTGCAG	3 cut(s)	151, 1087, 1382
BspMI	ACCTGC	1 cut(s)	1611
BspPI	GGATC	5 cut(s)	357, 582, 1528, 1891, 2412
BspT107I	GGYRCC	1 cut(s)	1571
BspTI	CTTAAG	1 cut(s)	2113
BsrDI	GCAATG	3 cut(s)	335, 335, 1523
BsrFI	RCCGGY	1 cut(s)	1930
BsrGI	TGTACA	1 cut(s)	2395
BsrI	ACTGG	4 cut(s)	72, 1199, 1909, 2168
BssAI	RCCGGY	1 cut(s)	1930
BssECI	CCNNGG	5 cut(s)	161, 1503, 1611, 2205, 2215
BssNAI	GTATAC	1 cut(s)	178
BssNI	GRCGYC	1 cut(s)	2043
BssSI	CACGAG	1 cut(s)	498
BssT1I	CCWWGG	4 cut(s)	1503, 1611, 2205, 2215
Bst1107I	GTATAC	1 cut(s)	178
Bst2BI	CACGAG	1 cut(s)	498
Bst2UI	CCWGG	4 cut(s)	471, 597, 1242, 2057
Bst4CI	ACNGT	5 cut(s)	995, 1579, 1821, 2013, 2286
Bst6I	CTCTTC	1 cut(s)	51
BstACI	GRCGYC	1 cut(s)	2043
BstAFI	CTTAAG	1 cut(s)	2113
BstAPI	GCANNNNNTGC	2 cut(s)	125, 1712
BstAUI	TGTACA	1 cut(s)	2395
BstBAI	YACGTR	1 cut(s)	1646
BstC8I	GCNNGC	2 cut(s)	1654, 1765
BstDEI	CTNAG	8 cut(s)	38, 522, 1046, 1059, 1215, 1670, 1701, 1873
BstDSI	CCRYGG	1 cut(s)	161
BstENI	CCTNNNNNAGG	1 cut(s)	1566
BstF5I	GGATG	6 cut(s)	268, 409, 746, 808, 1588, 1606
BstFNI	CGCG	1 cut(s)	163
BstHHI	GCGC	2 cut(s)	97, 1347
BstMAI	GTCTC	2 cut(s)	53, 1433
BstMWI	GCNNNNNNNGC	8 cut(s)	125, 131, 134, 140, 149, 376, 1712, 1773
BstNI	CCWGG	4 cut(s)	471, 597, 1242, 2057
BstNSI	RCATGY	4 cut(s)	751, 1072, 1767, 1951
BstSCI	CCNGG	4 cut(s)	469, 595, 1240, 2055
BstSFI	CTRYAG	5 cut(s)	129, 147, 1083, 1305, 1378
BstSNI	TACGTA	1 cut(s)	1646
BstUI	CGCG	1 cut(s)	163
BstV1I	GCAGC	7 cut(s)	112, 161, 543, 565, 731, 1329, 2188
BstX2I	RGATCY	3 cut(s)	574, 958, 2404
BstYI	RGATCY	3 cut(s)	574, 958, 2404
BstZ17I	GTATAC	1 cut(s)	178
Bsu36I	CCTNAGG	1 cut(s)	522
BsuRI	GGCC	7 cut(s)	83, 137, 166, 1464, 1502, 1871, 2167
BtgI	CCRYGG	1 cut(s)	161
BtgZI	GCGATG	1 cut(s)	1437
BtsCI	GGATG	6 cut(s)	268, 409, 746, 808, 1588, 1606
BtsI	GCAGTG	1 cut(s)	214
BtsIMutI	CAGTG	7 cut(s)	214, 1000, 1575, 1817, 1881, 1916, 2175
BveI	ACCTGC	1 cut(s)	1611
Cac8I	GCNNGC	2 cut(s)	1654, 1765
CaiI	CAGNNNCTG	1 cut(s)	125
CfoI	GCGC	2 cut(s)	97, 1347
Cfr10I	RCCGGY	1 cut(s)	1930
Cfr13I	GGNCC	4 cut(s)	81, 467, 1500, 1597
Cfr42I	CCGCGG	1 cut(s)	164
CseI	GACGC	2 cut(s)	1973, 2051
CsiI	ACCWGGT	1 cut(s)	2055
Csp6I	GTAC	7 cut(s)	1543, 1572, 1647, 2009, 2173, 2282, 2396
CspCI	CAANNNNNGTGG	2 cut(s)	2118, 2153
CviQI	GTAC	7 cut(s)	1543, 1572, 1647, 2009, 2173, 2282, 2396
DdeI	CTNAG	8 cut(s)	38, 522, 1046, 1059, 1215, 1670, 1701, 1873
DraI	TTTAAA	2 cut(s)	363, 679
DraIII	CACNNNGTG	1 cut(s)	1712
DrdI	GACNNNNNNGTC	1 cut(s)	1970
DseDI	GACNNNNNNGTC	1 cut(s)	1970
EaeI	YGGCCR	2 cut(s)	164, 1462
Eam1104I	CTCTTC	1 cut(s)	51
EarI	CTCTTC	1 cut(s)	51
EciI	GGCGGA	1 cut(s)	1889
Eco105I	TACGTA	1 cut(s)	1646
Eco130I	CCWWGG	4 cut(s)	1503, 1611, 2205, 2215
Eco24I	GRGCYC	1 cut(s)	1658
Eco47I	GGWCC	2 cut(s)	467, 1597
Eco57I	CTGAAG	6 cut(s)	206, 297, 1014, 1041, 1278, 2336
Eco81I	CCTNAGG	1 cut(s)	522
EcoNI	CCTNNNNNAGG	1 cut(s)	1566
EcoO109I	RGGNCCY	1 cut(s)	81
EcoRI	GAATTC	1 cut(s)	1694
EcoRII	CCWGG	4 cut(s)	469, 595, 1240, 2055
EcoT14I	CCWWGG	4 cut(s)	1503, 1611, 2205, 2215
EcoT22I	ATGCAT	1 cut(s)	1769
EcoT38I	GRGCYC	1 cut(s)	1658
ErhI	CCWWGG	4 cut(s)	1503, 1611, 2205, 2215
Esp3I	CGTCTC	2 cut(s)	53, 1433
FaqI	GGGAC	2 cut(s)	678, 1958
FauI	CCCGC	1 cut(s)	1645
FauNDI	CATATG	1 cut(s)	1769
FblI	GTMKAC	2 cut(s)	177, 1272
FokI	GGATG	6 cut(s)	255, 396, 753, 795, 1575, 1593
FriOI	GRGCYC	1 cut(s)	1658
FspBI	CTAG	5 cut(s)	315, 1398, 1566, 2216, 2343
FspI	TGCGCA	1 cut(s)	96
GlaI	GCGC	2 cut(s)	96, 1346
GsuI	CTGGAG	1 cut(s)	618
HaeIII	GGCC	7 cut(s)	83, 137, 166, 1464, 1502, 1871, 2167
HapII	CCGG	2 cut(s)	545, 1931
HgaI	GACGC	2 cut(s)	1973, 2051
HhaI	GCGC	2 cut(s)	97, 1347
Hin1I	GRCGYC	1 cut(s)	2043
Hin6I	GCGC	2 cut(s)	95, 1345
HinP1I	GCGC	2 cut(s)	95, 1345
HincII	GTYRAC	2 cut(s)	1167, 2041
HindII	GTYRAC	2 cut(s)	1167, 2041
HindIII	AAGCTT	2 cut(s)	2132, 2366
HinfI	GANTC	8 cut(s)	490, 725, 1123, 1370, 1472, 1687, 2240, 2333
HpaII	CCGG	2 cut(s)	545, 1931
HphI	GGTGA	2 cut(s)	1010, 2071
Hpy166II	GTNNAC	9 cut(s)	178, 218, 1167, 1273, 1636, 2041, 2173, 2264, 2377
Hpy188I	TCNGA	9 cut(s)	225, 489, 707, 1033, 1369, 1477, 1671, 1896, 2316
Hpy188III	TCNNGA	8 cut(s)	275, 302, 545, 780, 1493, 1691, 2348, 2425
Hpy8I	GTNNAC	9 cut(s)	178, 218, 1167, 1273, 1636, 2041, 2173, 2264, 2377
Hpy99I	CGWCG	1 cut(s)	187
HpyAV	CCTTC	6 cut(s)	642, 822, 1167, 1931, 2311, 2422
HpyCH4III	ACNGT	5 cut(s)	995, 1579, 1821, 2013, 2286
HpyCH4IV	ACGT	3 cut(s)	1275, 1645, 2280
HpyF10VI	GCNNNNNNNGC	8 cut(s)	125, 131, 134, 140, 149, 376, 1712, 1773
HpyF3I	CTNAG	8 cut(s)	38, 522, 1046, 1059, 1215, 1670, 1701, 1873
HpySE526I	ACGT	3 cut(s)	1275, 1645, 2280
Hsp92I	GRCGYC	1 cut(s)	2043
HspAI	GCGC	2 cut(s)	95, 1345
Kpn2I	TCCGGA	1 cut(s)	544
KpnI	GGTACC	1 cut(s)	1575
KspI	CCGCGG	1 cut(s)	164
LmnI	GCTCC	5 cut(s)	670, 684, 1419, 1781, 1814
Lsp1109I	GCAGC	7 cut(s)	112, 161, 543, 565, 731, 1329, 2188
LweI	GCATC	4 cut(s)	731, 924, 1460, 1615
MabI	ACCWGGT	1 cut(s)	2055
MaeI	CTAG	5 cut(s)	315, 1398, 1566, 2216, 2343
MaeII	ACGT	3 cut(s)	1275, 1645, 2280
MaeIII	GTNAC	4 cut(s)	228, 241, 891, 1124
MfeI	CAATTG	1 cut(s)	2031
MflI	RGATCY	3 cut(s)	574, 958, 2404
MhlI	GDGCHC	2 cut(s)	1658, 1819
MlsI	TGGCCA	1 cut(s)	1464
MluNI	TGGCCA	1 cut(s)	1464
MlyI	GAGTC	3 cut(s)	1132, 1696, 2327
MmeI	TCCRAC	3 cut(s)	289, 915, 1803
Mox20I	TGGCCA	1 cut(s)	1464
Mph1103I	ATGCAT	1 cut(s)	1769
MroI	TCCGGA	1 cut(s)	544
MroXI	GAANNNNTTC	2 cut(s)	631, 657
MscI	TGGCCA	1 cut(s)	1464
MslI	CAYNNNNRTG	3 cut(s)	900, 1768, 2384
Msp20I	TGGCCA	1 cut(s)	1464
MspA1I	CMGCKG	2 cut(s)	163, 556
MspCI	CTTAAG	1 cut(s)	2113
MspI	CCGG	2 cut(s)	545, 1931
MspR9I	CCNGG	4 cut(s)	471, 597, 1242, 2057
MunI	CAATTG	1 cut(s)	2031
Mva1269I	GAATGC	4 cut(s)	864, 1258, 1330, 2183
MvaI	CCWGG	4 cut(s)	471, 597, 1242, 2057
MvnI	CGCG	1 cut(s)	163
MwoI	GCNNNNNNNGC	8 cut(s)	125, 131, 134, 140, 149, 376, 1712, 1773
NdeI	CATATG	1 cut(s)	1769
NlaIV	GGNNCC	4 cut(s)	82, 879, 1573, 1777
NmuCI	GTSAC	1 cut(s)	1124
NsbI	TGCGCA	1 cut(s)	96
NsiI	ATGCAT	1 cut(s)	1769
NspI	RCATGY	4 cut(s)	751, 1072, 1767, 1951
PaeI	GCATGC	1 cut(s)	1767
PaqCI	CACCTGC	1 cut(s)	1611
PcsI	WCGNNNNNNNCGW	1 cut(s)	1682
PctI	GAATGC	4 cut(s)	864, 1258, 1330, 2183
PdmI	GAANNNNTTC	2 cut(s)	631, 657
PfeI	GAWTC	5 cut(s)	490, 725, 1370, 1472, 2240
PfoI	TCCNGGA	2 cut(s)	469, 595
PleI	GAGTC	3 cut(s)	1131, 1695, 2327
PpsI	GAGTC	3 cut(s)	1131, 1695, 2327
Ppu21I	YACGTR	1 cut(s)	1646
Psp6I	CCWGG	4 cut(s)	469, 595, 1240, 2055
PspGI	CCWGG	4 cut(s)	469, 595, 1240, 2055
PspN4I	GGNNCC	4 cut(s)	82, 879, 1573, 1777
PspPI	GGNCC	4 cut(s)	81, 467, 1500, 1597
PstI	CTGCAG	3 cut(s)	151, 1087, 1382
PstNI	CAGNNNCTG	1 cut(s)	125
PsuI	RGATCY	3 cut(s)	574, 958, 2404
PvuII	CAGCTG	1 cut(s)	556
RsaI	GTAC	7 cut(s)	1544, 1573, 1648, 2010, 2174, 2283, 2397
RsaNI	GTAC	7 cut(s)	1543, 1572, 1647, 2009, 2173, 2282, 2396
RseI	CAYNNNNRTG	3 cut(s)	900, 1768, 2384
SacII	CCGCGG	1 cut(s)	164
Sau96I	GGNCC	4 cut(s)	81, 467, 1500, 1597
ScaI	AGTACT	1 cut(s)	1544
SchI	GAGTC	3 cut(s)	1132, 1696, 2327
ScrFI	CCNGG	4 cut(s)	471, 597, 1242, 2057
SduI	GDGCHC	2 cut(s)	1658, 1819
SexAI	ACCWGGT	1 cut(s)	2055
SfaNI	GCATC	4 cut(s)	731, 924, 1460, 1615
SfcI	CTRYAG	5 cut(s)	129, 147, 1083, 1305, 1378
Sfr303I	CCGCGG	1 cut(s)	164
SgrBI	CCGCGG	1 cut(s)	164
SinI	GGWCC	2 cut(s)	467, 1597
SmiMI	CAYNNNNRTG	3 cut(s)	900, 1768, 2384
SmlI	CTYRAG	2 cut(s)	871, 2113
SmoI	CTYRAG	2 cut(s)	871, 2113
SnaBI	TACGTA	1 cut(s)	1646
SphI	GCATGC	1 cut(s)	1767
SsiI	CCGC	8 cut(s)	134, 161, 163, 325, 1072, 1245, 1652, 1900
SspMI	CTAG	5 cut(s)	315, 1398, 1566, 2216, 2343
StyD4I	CCNGG	4 cut(s)	469, 595, 1240, 2055
StyI	CCWWGG	4 cut(s)	1503, 1611, 2205, 2215
TaaI	ACNGT	5 cut(s)	995, 1579, 1821, 2013, 2286
TaiI	ACGT	3 cut(s)	1278, 1648, 2283
TaqI	TCGA	7 cut(s)	185, 516, 756, 2015, 2252, 2331, 2353
TatI	WGTACW	3 cut(s)	1542, 2008, 2395
TauI	GCSGC	3 cut(s)	137, 166, 328
TfiI	GAWTC	5 cut(s)	490, 725, 1370, 1472, 2240
TscAI	CASTG	7 cut(s)	214, 1000, 1582, 1824, 1881, 1916, 2175
TseFI	GTSAC	1 cut(s)	1124
TseI	GCWGC	7 cut(s)	125, 149, 553, 556, 744, 1342, 2201
Tsp45I	GTSAC	1 cut(s)	1124
TspGWI	ACGGA	4 cut(s)	116, 1457, 2249, 2417
TspRI	CASTG	7 cut(s)	214, 1000, 1582, 1824, 1881, 1916, 2175
Vha464I	CTTAAG	1 cut(s)	2113
VpaK11BI	GGWCC	2 cut(s)	467, 1597
XagI	CCTNNNNNAGG	1 cut(s)	1566
XapI	RAATTY	6 cut(s)	13, 654, 1003, 1153, 1411, 1694
XceI	RCATGY	4 cut(s)	751, 1072, 1767, 1951
XmaJI	CCTAGG	1 cut(s)	2215
XmiI	GTMKAC	2 cut(s)	177, 1272
XmnI	GAANNNNTTC	2 cut(s)	631, 657
XspI	CTAG	5 cut(s)	315, 1398, 1566, 2216, 2343
ZrmI	AGTACT	1 cut(s)	1544
Zsp2I	ATGCAT	1 cut(s)	1769

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.