MD02G1115000.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005501
Physical Location & Seq
Reverse (-)
9340193 .. 9345362
5170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1115000.v1.1.491

Sequence Viewer

Length: 2595 bp
ATGGGAGGCTCATTGCACCTTTTAAATTTCAACCAGGGCAGCATGGCCAGAAAGATTTTTACACATAAGAAGCATGATGGTGGCCTTGATGCTCCAAGAAATAGCCTGGAAATGCAGGTAGAACCTCAGAGCTATTGCGATGCAGGAGATTTGCCGGTTGAAGAAGACTGGTCTAAAAAGAATAATCCATTGGAACAAAGTTCAATGAAGAAATTAATTAATGGCGAAATATCCAAACATTCAAGTACCAGAAAAAATGCACCAAACATCGTGGCTCAACTGATGGGAATGGATATGTTTCCAGTGGAACCGAAATCTGCAGTTCAGCCTCTGGAAGAAAATGGAAAAAGTAGGAGAACGAAGTCTTTGAAAAAGGAAATTAATGGAAAGACCTCACTTGGTCATGTTTCTTCTAAGCTAAATTCTTCAGGGCAGATGGATCTTGATTCGTATTATCACAGTAATGAGAGAGATGCCACTAGGTGGAGCGATGACCAGAAAATCGAATATCCAAGGCGTAGAGTACATCCTCAAGAAGAAGAACTGAAAAAGTTCAAGAAAGAATTTGAAGCCTGGCAAGCTTCCAGGTTTAGGGAATGCTCAAGGATTGTCAAAATTGACAGCGACCTCAACGAGGAAAAGATGGTGGTTTCAGGGCATAGAGCAACGGAGAGAACATTAGAACATAAGGATCACGCGGTCAAAGCAATCTCGCATGAAGGTGGTGTTTTGGAACGTAGAGGAGATGAGTTTTCAGAACTATTCCCAGCCGAGCATACGGAGTATTTATCTTCAAGAAGCAGAAGAACCATGAGCTTAGACTTTGAGAAATCTTCCCTGATGAACTGTAAGAAGAGATTCGAGTCATTCAACACATCTTCTGCTCCTACAAGGATAGTTATCTTAAGACCTGGTCCTGATAGATTTTGCAACCATGAAGAGACTTGGGCAGGTTCTTCAAACACTCTAGAGAGGAAAGGTGGCATAGAAGATTTTCTCGAGGAGGTGAAAGAACGACTGAGATGCGACCTGCGAGGGAAAATGCATAAAAGGGGTTCTGTTGTCCGAGGAAGTGGAATTGAGACGCCATTTAGCGAACAGCCATCAGCTCCGAAGCAAATTGCTCGACACATTGCAAACCAGGTCAGAGAAAGTGTGACTAGGGACCTTGGCATGAATTTGCCGAGATCAGAATCGACAAGATCATACAGAAGCGAAATGCAGTTCAATGGATCAGGTTGCCCGGAGTTAATGCAGAGAGATACTAAAAGAATTTTGTTAGAGGGACTAAGAAATGCTTCAAAAATAGAAACAGAACTTCGTGATCCTGTATTAGTTTCCGGGAGATCTAGGTTATCTGCATTAGACAACGAAAGGGAAAGGGGAATGGTGAAATCTGAACAAGAGAAAAACAGATCATTCAGACATGGATCACATGAAGATGAGGTGCTTGATAGAGAGTTGTCGCCTAGAAATTTCATTAGGTCTCTATCCACCCCAGTACCTGGAACATCCTTTGGGAAACTTCTTCTTGAGGACCGCCTTGTTTTAACCGGTGCTCACATTCAGAGGAAGCATGAAGGCATTGGACATGTGTCCATGGAGATGAAACGTCAGAAGAAAGAAAGGTTTAATTTTAAAGACAAAGTTTCCAATTTCCGATACAGTTTTATGCTTAGGGGAAGACTTTTTGGCAAAAAAAATCAATCAGTTCTGGAATCACTTCATATTGAACGGTATCCTATGAAGGATATCTTGAGTGGCCCAACAGTGATTAAGAACTCTGGAGAGAGACATGATAATTTCACTGAGGTGCCTCCTAGTCCTGCATCTCTGTGCAGCACTGCTCAGGAAGATTTCTGGAGGCCAACTGATTATCTTAGTCCAATATCAACTCCGAGTGCAACTCCACGAGAAGATAGTATCATGCCACAGGCTTTTAGAGACATCAGCGATAATCTGAGGGAGTTGCGAAGACAACTGAACCAACTCGAGTCTAATGAGCCTGAGGACATAAAAGTTAAGGAAACGGTTGAGTCTGAAATGGCTAGGTTAGACAACCCCGCAGAGGTTTACATACGAGATTTGCTTGTCGCTTCTGGTTTGTATGATGGATCATTTGAGAAATCAATGTTGAGATGGGACACATTTGCGAAGCCAATCAGCAACTCAGTGTTCGAAGAAGTGGAAGAATCGTATAAAAACTTGGCCAACAAGGAAGATCCTTCAGCAAAAGATCATAATGAGAGGGTAGATCACAAGTTGTTACGCGATCTGTTGAACGAAGCTCTTTCAACTGTTCTCAGACCACCAACAAACATGTCTAAATTCAGGAGAAAAATGATCAGCTCCTCCGCGCTGCCAGCGTTGTGCGGCAAGAAGTTGTTGAATTGTGTGTGGGGAATCATCAGCGAGTGCCTGTACCCTCCAACTGATGTACCCTATTACTCGCTTGATGATATGGCAGCCCGGGACCTGGGATCAGCTCCTTGGTCGAGATTGATGGACGAGGACGTTAACGCTCTGGTAAAAGAGATGGAAGGCCTAATCATTGGGGATCTTGTGCAGGAAATTTTGGATGAACTGAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

865

Amino Acids

98.77

Weight (kDa)

7.36

Isoelectric Point (pI)

57.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 691 - 857 3.1e-32 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 106, 941, 1038
AccB1I GGYRCC 1 cut(s) 1813
AccB7I CCANNNNNTGG 2 cut(s) 483, 1505
AccII CGCG 3 cut(s) 698, 2271, 2357
AciI CCGC 5 cut(s) 698, 1540, 2064, 2355, 2373
AclWI GGATC 9 cut(s) 447, 699, 1240, 1319, 1438, 2122, 2216, 2488, 2565
AcoI YGGCCR 2 cut(s) 45, 2208
AcsI RAATTY 8 cut(s) 25, 421, 563, 1177, 1272, 1474, 2327, 2571
AcuI CTGAAG 2 cut(s) 411, 2211
AcyI GRCGYC 1 cut(s) 1085
AdeI CACNNNGTG 1 cut(s) 483
AfaI GTAC 5 cut(s) 247, 525, 1503, 2423, 2439
AfiI CCNNNNNNNGG 6 cut(s) 483, 591, 634, 1505, 2068, 2476
AflII CTTAAG 1 cut(s) 904
AflIII ACRYGT 2 cut(s) 1591, 2319
AgeI ACCGGT 1 cut(s) 1553
AjnI CCWGG 8 cut(s) 33, 105, 572, 584, 910, 1140, 1504, 2475
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AleI CACNNNNGTG 1 cut(s) 1811
AluBI AGCT 8 cut(s) 132, 418, 581, 816, 1109, 2288, 2349, 2486
AluI AGCT 8 cut(s) 132, 418, 581, 816, 1109, 2288, 2349, 2486
Alw21I GWGCWC 1 cut(s) 1561
Alw26I GTCTC 5 cut(s) 935, 1076, 1491, 1786, 1938
AlwI GGATC 9 cut(s) 447, 699, 1240, 1319, 1438, 2122, 2216, 2488, 2565
AlwNI CAGNNNCTG 2 cut(s) 331, 1505
Ama87I CYCGRG 3 cut(s) 998, 1991, 2469
AoxI GGCC 6 cut(s) 45, 82, 1762, 1865, 2208, 2542
ApeKI GCWGC 4 cut(s) 39, 1839, 2359, 2465
ApoI RAATTY 8 cut(s) 25, 421, 563, 1177, 1272, 1474, 2327, 2571
AseI ATTAAT 3 cut(s) 215, 219, 381
AsiGI ACCGGT 1 cut(s) 1553
Asp700I GAANNNNTTC 5 cut(s) 551, 857, 993, 1297, 1722
AspLEI GCGC 1 cut(s) 2359
AspS9I GGNCC 5 cut(s) 914, 1165, 1537, 1763, 2473
AsuC2I CCSGG 4 cut(s) 1244, 1342, 2470, 2471
AsuHPI GGTGA 2 cut(s) 1018, 1402
AsuII TTCGAA 1 cut(s) 2178
AvaI CYCGRG 3 cut(s) 998, 1991, 2469
AvaII GGWCC 4 cut(s) 914, 1165, 1537, 2473
AxyI CCTNAGG 1 cut(s) 2007
BaeI ACNNNNGTAYC 4 cut(s) 1485, 1518, 2421, 2454
BalI TGGCCA 2 cut(s) 47, 2210
BanI GGYRCC 1 cut(s) 1813
BauI CACGAG 1 cut(s) 1911
BbsI GAAGAC 3 cut(s) 171, 1690, 1981
Bbv12I GWGCWC 1 cut(s) 1561
BbvI GCAGC 4 cut(s) 51, 1851, 2346, 2477
BccI CCATC 9 cut(s) 71, 277, 430, 637, 1111, 2105, 2133, 2497, 2530
BcgI CGANNNNNNTGC 4 cut(s) 1005, 1039, 1106, 1140
BciT130I CCWGG 8 cut(s) 35, 107, 574, 586, 912, 1142, 1506, 2477
BciVI GTATCC 1 cut(s) 1749
BclI TGATCA 1 cut(s) 2343
BcnI CCSGG 4 cut(s) 1244, 1342, 2470, 2471
BcoDI GTCTC 5 cut(s) 935, 1076, 1491, 1786, 1938
BfaI CTAG 7 cut(s) 480, 968, 1161, 1350, 1470, 1821, 2049
BfmI CTRYAG 1 cut(s) 318
BfrI CTTAAG 1 cut(s) 904
BfuAI ACCTGC 3 cut(s) 106, 941, 1038
BfuI GTATCC 1 cut(s) 1749
BglII AGATCT 1 cut(s) 1346
BisI GCNGC 5 cut(s) 40, 1840, 2360, 2374, 2466
BlsI GCNGC 5 cut(s) 41, 1841, 2361, 2375, 2467
Bme18I GGWCC 4 cut(s) 914, 1165, 1537, 2473
BmeT110I CYCGRG 3 cut(s) 998, 1991, 2469
BmgT120I GGNCC 5 cut(s) 914, 1165, 1537, 1763, 2473
BmiI GGNNCC 4 cut(s) 309, 1166, 1815, 2474
BmrI ACTGGG 1 cut(s) 1493
BmsI GCATC 5 cut(s) 79, 130, 463, 1013, 1838
BmuI ACTGGG 1 cut(s) 1493
BoxI GACNNNNGTC 1 cut(s) 1594
BpiI GAAGAC 3 cut(s) 171, 1690, 1981
BplI GAGNNNNNCTC 2 cut(s) 1891, 1923
BpmI CTGGAG 2 cut(s) 1806, 1882
Bpu10I CCTNAGC 2 cut(s) 1676, 1848
Bpu14I TTCGAA 1 cut(s) 2178
BpuEI CTTGAG 4 cut(s) 516, 586, 1553, 1777
BpuMI CCSGG 4 cut(s) 1244, 1342, 2470, 2471
BsaBI GATNNNNATC 2 cut(s) 899, 1192
BsaHI GRCGYC 1 cut(s) 1085
BsaI GGTCTC 1 cut(s) 1491
BsaJI CCNNGG 8 cut(s) 34, 512, 1066, 1168, 1599, 2469, 2476, 2489
BsaWI WCCGGW 1 cut(s) 1553
Bsc4I CCNNNNNNNGG 6 cut(s) 483, 591, 634, 1505, 2068, 2476
Bse118I RCCGGY 2 cut(s) 154, 1553
Bse1I ACTGG 3 cut(s) 173, 302, 1499
Bse21I CCTNAGG 1 cut(s) 2007
Bse3DI GCAATG 2 cut(s) 11, 1131
Bse8I GATNNNNATC 2 cut(s) 899, 1192
BseBI CCWGG 8 cut(s) 35, 107, 574, 586, 912, 1142, 1506, 2477
BseDI CCNNGG 8 cut(s) 34, 512, 1066, 1168, 1599, 2469, 2476, 2489
BseGI GGATG 3 cut(s) 526, 1511, 2584
BseJI GATNNNNATC 2 cut(s) 899, 1192
BseLI CCNNNNNNNGG 6 cut(s) 483, 591, 634, 1505, 2068, 2476
BseMI GCAATG 2 cut(s) 11, 1131
BseMII CTCAG 8 cut(s) 140, 1010, 1800, 1862, 1952, 1998, 2184, 2317
BseNI ACTGG 3 cut(s) 173, 302, 1499
BseRI GAGGAG 3 cut(s) 756, 1016, 2341
BseXI GCAGC 4 cut(s) 51, 1851, 2346, 2477
BseYI CCCAGC 1 cut(s) 766
BsgI GTGCAG 2 cut(s) 1858, 2585
Bsh1236I CGCG 3 cut(s) 698, 2271, 2357
BshFI GGCC 6 cut(s) 47, 84, 1764, 1867, 2210, 2544
BshNI GGYRCC 1 cut(s) 1813
BshTI ACCGGT 1 cut(s) 1553
BsiHKAI GWGCWC 1 cut(s) 1561
BsiHKCI CYCGRG 3 cut(s) 998, 1991, 2469
BsiSI CCGG 5 cut(s) 155, 1244, 1341, 1554, 2470
BslFI GGGAC 4 cut(s) 1178, 1299, 2156, 2486
BslI CCNNNNNNNGG 6 cut(s) 483, 591, 634, 1505, 2068, 2476
BsmAI GTCTC 5 cut(s) 935, 1076, 1491, 1786, 1938
BsmBI CGTCTC 1 cut(s) 1076
BsmFI GGGAC 4 cut(s) 1178, 1299, 2156, 2486
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 6 cut(s) 47, 84, 1764, 1867, 2210, 2544
Bso31I GGTCTC 1 cut(s) 1491
BsoBI CYCGRG 3 cut(s) 998, 1991, 2469
Bsp119I TTCGAA 1 cut(s) 2178
Bsp1286I GDGCHC 1 cut(s) 1561
Bsp19I CCATGG 1 cut(s) 1599
BspACI CCGC 5 cut(s) 698, 1540, 2064, 2355, 2373
BspANI GGCC 6 cut(s) 47, 84, 1764, 1867, 2210, 2544
BspCNI CTCAG 8 cut(s) 139, 1011, 1801, 1861, 1953, 1999, 2183, 2316
BspFNI CGCG 3 cut(s) 698, 2271, 2357
BspLI GGNNCC 4 cut(s) 309, 1166, 1815, 2474
BspMAI CTGCAG 1 cut(s) 322
BspMI ACCTGC 3 cut(s) 106, 941, 1038
BspPI GGATC 9 cut(s) 447, 699, 1240, 1319, 1438, 2122, 2216, 2488, 2565
BspT104I TTCGAA 1 cut(s) 2178
BspT107I GGYRCC 1 cut(s) 1813
BspTI CTTAAG 1 cut(s) 904
BspTNI GGTCTC 1 cut(s) 1491
BsrDI GCAATG 2 cut(s) 11, 1131
BsrFI RCCGGY 2 cut(s) 154, 1553
BsrI ACTGG 3 cut(s) 173, 302, 1499
BssAI RCCGGY 2 cut(s) 154, 1553
BssECI CCNNGG 8 cut(s) 34, 512, 1066, 1168, 1599, 2469, 2476, 2489
BssNI GRCGYC 1 cut(s) 1085
BssSI CACGAG 1 cut(s) 1911
BssT1I CCWWGG 4 cut(s) 512, 1168, 1599, 2489
Bst2BI CACGAG 1 cut(s) 1911
Bst2UI CCWGG 8 cut(s) 35, 107, 574, 586, 912, 1142, 1506, 2477
Bst4CI ACNGT 7 cut(s) 461, 848, 1667, 1737, 1771, 2032, 2299
Bst6I CTCTTC 2 cut(s) 848, 933
BstACI GRCGYC 1 cut(s) 1085
BstAFI CTTAAG 1 cut(s) 904
BstBI TTCGAA 1 cut(s) 2178
BstC8I GCNNGC 2 cut(s) 579, 2364
BstDSI CCRYGG 1 cut(s) 1599
BstENI CCTNNNNNAGG 1 cut(s) 632
BstF5I GGATG 3 cut(s) 526, 1511, 2584
BstFNI CGCG 3 cut(s) 698, 2271, 2357
BstHHI GCGC 1 cut(s) 2359
BstMAI GTCTC 5 cut(s) 935, 1076, 1491, 1786, 1938
BstMWI GCNNNNNNNGC 3 cut(s) 578, 704, 2363
BstNI CCWGG 8 cut(s) 35, 107, 574, 586, 912, 1142, 1506, 2477
BstNSI RCATGY 2 cut(s) 1595, 2323
BstPAI GACNNNNGTC 1 cut(s) 1594
BstSFI CTRYAG 1 cut(s) 318
BstUI CGCG 3 cut(s) 698, 2271, 2357
BstV1I GCAGC 4 cut(s) 51, 1851, 2346, 2477
BstV2I GAAGAC 3 cut(s) 171, 1690, 1981
BstX2I RGATCY 4 cut(s) 439, 1346, 2221, 2557
BstYI RGATCY 4 cut(s) 439, 1346, 2221, 2557
Bsu36I CCTNAGG 1 cut(s) 2007
BsuI GTATCC 1 cut(s) 1749
BsuRI GGCC 6 cut(s) 47, 84, 1764, 1867, 2210, 2544
BtgI CCRYGG 1 cut(s) 1599
BtgZI GCGATG 2 cut(s) 153, 504
BtsCI GGATG 3 cut(s) 526, 1511, 2584
BtsI GCAGTG 1 cut(s) 1842
BtsIMutI CAGTG 5 cut(s) 309, 1776, 1806, 1842, 2178
BveI ACCTGC 3 cut(s) 106, 941, 1038
Cac8I GCNNGC 2 cut(s) 579, 2364
CaiI CAGNNNCTG 2 cut(s) 331, 1505
CfoI GCGC 1 cut(s) 2359
Cfr10I RCCGGY 2 cut(s) 154, 1553
Cfr13I GGNCC 5 cut(s) 914, 1165, 1537, 1763, 2473
Cfr9I CCCGGG 1 cut(s) 2469
CseI GACGC 1 cut(s) 1093
CsiI ACCWGGT 2 cut(s) 910, 1140
Csp6I GTAC 5 cut(s) 246, 524, 1502, 2422, 2438
CspAI ACCGGT 1 cut(s) 1553
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviQI GTAC 5 cut(s) 246, 524, 1502, 2422, 2438
DraI TTTAAA 2 cut(s) 24, 1639
DraIII CACNNNGTG 1 cut(s) 483
EaeI YGGCCR 2 cut(s) 45, 2208
Eam1104I CTCTTC 2 cut(s) 848, 933
EarI CTCTTC 2 cut(s) 848, 933
Eco130I CCWWGG 4 cut(s) 512, 1168, 1599, 2489
Eco147I AGGCCT 1 cut(s) 2544
Eco31I GGTCTC 1 cut(s) 1491
Eco32I GATATC 1 cut(s) 1753
Eco47I GGWCC 4 cut(s) 914, 1165, 1537, 2473
Eco57I CTGAAG 2 cut(s) 411, 2211
Eco81I CCTNAGG 1 cut(s) 2007
Eco88I CYCGRG 3 cut(s) 998, 1991, 2469
EcoNI CCTNNNNNAGG 1 cut(s) 632
EcoO109I RGGNCCY 2 cut(s) 1165, 2473
EcoRII CCWGG 8 cut(s) 33, 105, 572, 584, 910, 1140, 1504, 2475
EcoRV GATATC 1 cut(s) 1753
EcoT14I CCWWGG 4 cut(s) 512, 1168, 1599, 2489
EcoT22I ATGCAT 1 cut(s) 1047
ErhI CCWWGG 4 cut(s) 512, 1168, 1599, 2489
Esp3I CGTCTC 1 cut(s) 1076
FalI AAGNNNNNCTT 4 cut(s) 1282, 1314, 1739, 1771
FaqI GGGAC 4 cut(s) 1178, 1299, 2156, 2486
FauI CCCGC 1 cut(s) 2071
FbaI TGATCA 1 cut(s) 2343
Fnu4HI GCNGC 5 cut(s) 40, 1840, 2360, 2374, 2466
FokI GGATG 3 cut(s) 513, 1498, 2591
Fsp4HI GCNGC 5 cut(s) 40, 1840, 2360, 2374, 2466
FspBI CTAG 7 cut(s) 480, 968, 1161, 1350, 1470, 1821, 2049
GlaI GCGC 1 cut(s) 2358
GluI GCNGC 5 cut(s) 40, 1840, 2360, 2374, 2466
GsaI CCCAGC 1 cut(s) 770
GsuI CTGGAG 2 cut(s) 1806, 1882
HaeIII GGCC 6 cut(s) 47, 84, 1764, 1867, 2210, 2544
HapII CCGG 5 cut(s) 155, 1244, 1341, 1554, 2470
HgaI GACGC 1 cut(s) 1093
HhaI GCGC 1 cut(s) 2359
Hin1I GRCGYC 1 cut(s) 1085
Hin6I GCGC 1 cut(s) 2357
HinP1I GCGC 1 cut(s) 2357
HincII GTYRAC 1 cut(s) 2518
HindII GTYRAC 1 cut(s) 2518
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 9 cut(s) 446, 858, 863, 1193, 1718, 1994, 2036, 2192, 2403
HpaI GTTAAC 1 cut(s) 2518
HpaII CCGG 5 cut(s) 155, 1244, 1341, 1554, 2470
HphI GGTGA 2 cut(s) 1018, 1402
Hpy166II GTNNAC 2 cut(s) 2074, 2518
Hpy8I GTNNAC 2 cut(s) 2074, 2518
HpyAV CCTTC 5 cut(s) 713, 1574, 1741, 2235, 2534
HpyCH4III ACNGT 7 cut(s) 461, 848, 1667, 1737, 1771, 2032, 2299
HpyCH4IV ACGT 3 cut(s) 736, 1612, 2514
HpyF10VI GCNNNNNNNGC 3 cut(s) 578, 704, 2363
HpySE526I ACGT 3 cut(s) 736, 1612, 2514
Hsp92I GRCGYC 1 cut(s) 1085
HspAI GCGC 1 cut(s) 2357
Ksp22I TGATCA 1 cut(s) 2343
KspAI GTTAAC 1 cut(s) 2518
LmnI GCTCC 6 cut(s) 97, 486, 889, 1114, 2354, 2491
Lsp1109I GCAGC 4 cut(s) 51, 1851, 2346, 2477
LweI GCATC 5 cut(s) 79, 130, 463, 1013, 1838
MabI ACCWGGT 2 cut(s) 910, 1140
MaeI CTAG 7 cut(s) 480, 968, 1161, 1350, 1470, 1821, 2049
MaeII ACGT 3 cut(s) 736, 1612, 2514
MaeIII GTNAC 2 cut(s) 1156, 2265
MflI RGATCY 4 cut(s) 439, 1346, 2221, 2557
MhlI GDGCHC 1 cut(s) 1561
MlsI TGGCCA 2 cut(s) 47, 2210
MluNI TGGCCA 2 cut(s) 47, 2210
MlyI GAGTC 3 cut(s) 872, 2003, 2045
MmeI TCCRAC 1 cut(s) 2453
Mox20I TGGCCA 2 cut(s) 47, 2210
Mph1103I ATGCAT 1 cut(s) 1047
MroXI GAANNNNTTC 5 cut(s) 551, 857, 993, 1297, 1722
MscI TGGCCA 2 cut(s) 47, 2210
MslI CAYNNNNRTG 7 cut(s) 78, 462, 720, 1440, 1604, 1811, 1834
Msp20I TGGCCA 2 cut(s) 47, 2210
MspCI CTTAAG 1 cut(s) 904
MspI CCGG 5 cut(s) 155, 1244, 1341, 1554, 2470
Mva1269I GAATGC 1 cut(s) 602
MvaI CCWGG 8 cut(s) 35, 107, 574, 586, 912, 1142, 1506, 2477
MvnI CGCG 3 cut(s) 698, 2271, 2357
MwoI GCNNNNNNNGC 3 cut(s) 578, 704, 2363
NciI CCSGG 4 cut(s) 1244, 1342, 2470, 2471
NcoI CCATGG 1 cut(s) 1599
NlaIV GGNNCC 4 cut(s) 309, 1166, 1815, 2474
NmeAIII GCCGAG 2 cut(s) 796, 1209
NmuCI GTSAC 1 cut(s) 1156
NsiI ATGCAT 1 cut(s) 1047
NspI RCATGY 2 cut(s) 1595, 2323
NspV TTCGAA 1 cut(s) 2178
OliI CACNNNNGTG 1 cut(s) 1811
PacI TTAATTAA 1 cut(s) 219
PaeR7I CTCGAG 2 cut(s) 998, 1991
PceI AGGCCT 1 cut(s) 2544
PciI ACATGT 2 cut(s) 1591, 2319
PctI GAATGC 1 cut(s) 602
PdmI GAANNNNTTC 5 cut(s) 551, 857, 993, 1297, 1722
PfeI GAWTC 6 cut(s) 446, 858, 1193, 1718, 2192, 2403
PflFI GACNNNGTC 1 cut(s) 912
PflMI CCANNNNNTGG 2 cut(s) 483, 1505
PfoI TCCNGGA 1 cut(s) 1340
PinAI ACCGGT 1 cut(s) 1553
PkrI GCNGC 5 cut(s) 41, 1841, 2361, 2375, 2467
PleI GAGTC 3 cut(s) 871, 2002, 2044
PpsI GAGTC 3 cut(s) 871, 2002, 2044
PpuMI RGGWCCY 2 cut(s) 1165, 2473
PscI ACATGT 2 cut(s) 1591, 2319
PshAI GACNNNNGTC 1 cut(s) 1594
PshBI ATTAAT 3 cut(s) 215, 219, 381
Psp5II RGGWCCY 2 cut(s) 1165, 2473
Psp6I CCWGG 8 cut(s) 33, 105, 572, 584, 910, 1140, 1504, 2475
PspFI CCCAGC 1 cut(s) 766
PspGI CCWGG 8 cut(s) 33, 105, 572, 584, 910, 1140, 1504, 2475
PspN4I GGNNCC 4 cut(s) 309, 1166, 1815, 2474
PspPI GGNCC 5 cut(s) 914, 1165, 1537, 1763, 2473
PspPPI RGGWCCY 2 cut(s) 1165, 2473
PspXI VCTCGAGB 1 cut(s) 1991
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 2 cut(s) 331, 1505
PsuI RGATCY 4 cut(s) 439, 1346, 2221, 2557
PsyI GACNNNGTC 1 cut(s) 912
RsaI GTAC 5 cut(s) 247, 525, 1503, 2423, 2439
RsaNI GTAC 5 cut(s) 246, 524, 1502, 2422, 2438
RseI CAYNNNNRTG 7 cut(s) 78, 462, 720, 1440, 1604, 1811, 1834
SatI GCNGC 5 cut(s) 40, 1840, 2360, 2374, 2466
Sau96I GGNCC 5 cut(s) 914, 1165, 1537, 1763, 2473
SchI GAGTC 3 cut(s) 872, 2003, 2045
SduI GDGCHC 1 cut(s) 1561
SexAI ACCWGGT 2 cut(s) 910, 1140
SfaNI GCATC 5 cut(s) 79, 130, 463, 1013, 1838
SfcI CTRYAG 1 cut(s) 318
Sfr274I CTCGAG 2 cut(s) 998, 1991
SfuI TTCGAA 1 cut(s) 2178
SinI GGWCC 4 cut(s) 914, 1165, 1537, 2473
SlaI CTCGAG 2 cut(s) 998, 1991
SmaI CCCGGG 1 cut(s) 2471
SmiMI CAYNNNNRTG 7 cut(s) 78, 462, 720, 1440, 1604, 1811, 1834
SmlI CTYRAG 7 cut(s) 531, 601, 904, 998, 1532, 1756, 1991
SmoI CTYRAG 7 cut(s) 531, 601, 904, 998, 1532, 1756, 1991
SseBI AGGCCT 1 cut(s) 2544
SsiI CCGC 5 cut(s) 698, 1540, 2064, 2355, 2373
SspMI CTAG 7 cut(s) 480, 968, 1161, 1350, 1470, 1821, 2049
StuI AGGCCT 1 cut(s) 2544
StyI CCWWGG 4 cut(s) 512, 1168, 1599, 2489
TaaI ACNGT 7 cut(s) 461, 848, 1667, 1737, 1771, 2032, 2299
TaiI ACGT 3 cut(s) 739, 1615, 2517
TaqI TCGA 8 cut(s) 504, 861, 999, 1126, 1196, 1992, 2178, 2495
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 2376
TfiI GAWTC 6 cut(s) 446, 858, 1193, 1718, 2192, 2403
TscAI CASTG 5 cut(s) 309, 1776, 1813, 1849, 2178
TseFI GTSAC 1 cut(s) 1156
TseI GCWGC 4 cut(s) 39, 1839, 2359, 2465
Tsp45I GTSAC 1 cut(s) 1156
TspGWI ACGGA 2 cut(s) 683, 794
TspMI CCCGGG 1 cut(s) 2469
TspRI CASTG 5 cut(s) 309, 1776, 1813, 1849, 2178
Tth111I GACNNNGTC 1 cut(s) 912
Van91I CCANNNNNTGG 2 cut(s) 483, 1505
Vha464I CTTAAG 1 cut(s) 904
VpaK11BI GGWCC 4 cut(s) 914, 1165, 1537, 2473
VspI ATTAAT 3 cut(s) 215, 219, 381
XagI CCTNNNNNAGG 1 cut(s) 632
XapI RAATTY 8 cut(s) 25, 421, 563, 1177, 1272, 1474, 2327, 2571
XbaI TCTAGA 1 cut(s) 967
XceI RCATGY 2 cut(s) 1595, 2323
XhoI CTCGAG 2 cut(s) 998, 1991
XmaI CCCGGG 1 cut(s) 2469
XmnI GAANNNNTTC 5 cut(s) 551, 857, 993, 1297, 1722
XspI CTAG 7 cut(s) 480, 968, 1161, 1350, 1470, 1821, 2049
Zsp2I ATGCAT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.