Rorug02G0064400

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005501
Physical Location & Seq
Reverse (-)
5069169 .. 5069834
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0064400.1

Sequence Viewer

Length: 666 bp
ATGAGTTTTAAGGAATGGATGCTTGAAAGAGCACAACACCTCAAACCTGACACCTTCGAGAAGCTCATGATGATTATTTGGGCTTTATGGAAAAACCGTAACAATGTGCTATGGGAAGGAAAGGCTCAATCTGTTCCTGATTTGGTTTTGGGTTGTTTTACATGGCTCACTGAGTTCCAGAAATCCAGAGGAACAGGTACATCCACTCAAAGATCGGTCCGGCCAAAGTGGAAACCACCAACGCAGGGTTTCAAATTGAATGTTGATGCCGCCTTCCTACCAAATCAGCATCAGGGTGGACTCGGGGGAGTATTGAGGGACTCGACAGGTCAGTTCAAAGCTGCTTTCAGTAAGCTGCATGTTGACCATGCAGCTTCACCAAAGCAGTGTGAGCTGCTAGCCATTAGGGCGAGTTTGGATTTGTTATCCTCCTTAGATATACAAAATGTGTGTATAGAAAGTGATTGCATGGAAGCTGTCTACGAAGCAAACTGTACGAATTACGCTTTATTAGCTAATGGAGGTCTAGTGGATGATATTCAGGCAATGTGGCGTAGGCTGCAAGGGGTGACCATATGTCATACTCCGAGAACCTGTAATGCGGTGGCTGTAACGTCCCGAACCCAAATTCACCGGTTTACTAGTCATTTGGACAGTAAACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

221

Amino Acids

24.9

Weight (kDa)

9.02

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 87 - 204 1.4e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 480
AciI CCGC 2 cut(s) 270, 602
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 627
AfaI GTAC 2 cut(s) 199, 496
AfiI CCNNNNNNNGG 1 cut(s) 245
AgeI ACCGGT 1 cut(s) 633
AgsI TTSAA 4 cut(s) 26, 253, 259, 337
AhdI GACNNNNNGTC 1 cut(s) 576
AhlI ACTAGT 1 cut(s) 641
AluBI AGCT 7 cut(s) 64, 341, 355, 374, 394, 476, 515
AluI AGCT 7 cut(s) 64, 341, 355, 374, 394, 476, 515
Alw21I GWGCWC 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 5 cut(s) 341, 355, 371, 394, 559
ApoI RAATTY 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 633
AspS9I GGNCC 1 cut(s) 217
AsuHPI GGTGA 3 cut(s) 369, 580, 623
AsuNHI GCTAGC 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 5 cut(s) 328, 342, 381, 383, 546
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 3 cut(s) 398, 527, 642
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 6 cut(s) 270, 342, 356, 372, 395, 560
BlsI GCNGC 6 cut(s) 271, 343, 357, 373, 396, 561
Bme18I GGWCC 1 cut(s) 217
BmeRI GACNNNNNGTC 1 cut(s) 576
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 217
BmsI GCATC 3 cut(s) 9, 256, 298
BmtI GCTAGC 1 cut(s) 401
BsaWI WCCGGW 1 cut(s) 633
BsaXI ACNNNNNCTCC 2 cut(s) 513, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 633
Bse3DI GCAATG 1 cut(s) 552
BseGI GGATG 3 cut(s) 24, 200, 538
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 5 cut(s) 328, 342, 381, 383, 546
BshFI GGCC 1 cut(s) 223
BshTI ACCGGT 1 cut(s) 633
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 220, 634
BslFI GGGAC 2 cut(s) 332, 601
BslI CCNNNNNNNGG 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 332, 601
BsnI GGCC 1 cut(s) 223
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 1 cut(s) 212
BspACI CCGC 2 cut(s) 270, 602
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 1 cut(s) 163
BspHI TCATGA 1 cut(s) 66
BspOI GCTAGC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 633
BssAI RCCGGY 1 cut(s) 633
BssMI GATC 1 cut(s) 212
Bst4CI ACNGT 4 cut(s) 98, 494, 656, 663
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 171, 433
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 3 cut(s) 24, 200, 538
BstKTI GATC 1 cut(s) 215
BstMBI GATC 1 cut(s) 212
BstMWI GCNNNNNNNGC 4 cut(s) 391, 407, 512, 559
BstNSI RCATGY 1 cut(s) 362
BstPI GGTNACC 1 cut(s) 568
BstV1I GCAGC 5 cut(s) 328, 342, 381, 383, 546
BsuRI GGCC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 24, 200, 538
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 2 cut(s) 168, 392
Cac8I GCNNGC 1 cut(s) 399
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 1 cut(s) 217
CpoI CGGWCCG 1 cut(s) 217
Csp6I GTAC 2 cut(s) 198, 495
CspAI ACCGGT 1 cut(s) 633
CspI CGGWCCG 1 cut(s) 217
CviAII CATG 5 cut(s) 67, 162, 359, 368, 469
CviQI GTAC 2 cut(s) 198, 495
DdeI CTNAG 2 cut(s) 171, 433
DpnI GATC 1 cut(s) 214
DpnII GATC 1 cut(s) 212
DriI GACNNNNNGTC 1 cut(s) 576
EaeI YGGCCR 1 cut(s) 221
Eam1105I GACNNNNNGTC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 568
FaeI CATG 5 cut(s) 70, 165, 362, 371, 472
FaqI GGGAC 2 cut(s) 332, 601
FatI CATG 5 cut(s) 66, 161, 358, 367, 468
FauNDI CATATG 1 cut(s) 575
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 6 cut(s) 270, 342, 356, 372, 395, 560
FokI GGATG 3 cut(s) 31, 187, 545
Fsp4HI GCNGC 6 cut(s) 270, 342, 356, 372, 395, 560
FspBI CTAG 3 cut(s) 398, 527, 642
GluI GCNGC 6 cut(s) 270, 342, 356, 372, 395, 560
HaeIII GGCC 1 cut(s) 223
HapII CCGG 2 cut(s) 220, 634
Hin1II CATG 5 cut(s) 70, 165, 362, 371, 472
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 300, 320
HpaII CCGG 2 cut(s) 220, 634
HphI GGTGA 3 cut(s) 369, 580, 623
Hpy166II GTNNAC 5 cut(s) 299, 364, 481, 639, 659
Hpy188I TCNGA 1 cut(s) 588
Hpy188III TCNNGA 6 cut(s) 58, 67, 137, 178, 186, 618
Hpy8I GTNNAC 5 cut(s) 299, 364, 481, 639, 659
HpyAV CCTTC 3 cut(s) 64, 110, 283
HpyCH4III ACNGT 4 cut(s) 98, 494, 656, 663
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 4 cut(s) 358, 371, 468, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 391, 407, 512, 559
HpyF3I CTNAG 2 cut(s) 171, 433
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 5 cut(s) 70, 165, 362, 371, 472
Kzo9I GATC 1 cut(s) 212
Lsp1109I GCAGC 5 cut(s) 328, 342, 381, 383, 546
LweI GCATC 3 cut(s) 9, 256, 298
MaeI CTAG 3 cut(s) 398, 527, 642
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 3 cut(s) 98, 568, 610
MalI GATC 1 cut(s) 214
MboI GATC 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 3 cut(s) 254, 499, 627
MlyI GAGTC 2 cut(s) 294, 314
MnlI CCTC 5 cut(s) 50, 182, 309, 439, 515
MseI TTAA 1 cut(s) 9
MslI CAYNNNNRTG 1 cut(s) 294
MspI CCGG 2 cut(s) 220, 634
MwoI GCNNNNNNNGC 4 cut(s) 391, 407, 512, 559
NdeI CATATG 1 cut(s) 575
NdeII GATC 1 cut(s) 212
NheI GCTAGC 1 cut(s) 397
NlaIII CATG 5 cut(s) 70, 165, 362, 371, 472
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 1 cut(s) 362
PagI TCATGA 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 633
PkrI GCNGC 6 cut(s) 271, 343, 357, 373, 396, 561
PleI GAGTC 2 cut(s) 294, 314
PpsI GAGTC 2 cut(s) 294, 314
PspEI GGTNACC 1 cut(s) 568
PspPI GGNCC 1 cut(s) 217
RsaI GTAC 2 cut(s) 199, 496
RsaNI GTAC 2 cut(s) 198, 495
RseI CAYNNNNRTG 1 cut(s) 294
Rsr2I CGGWCCG 1 cut(s) 217
RsrII CGGWCCG 1 cut(s) 217
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 6 cut(s) 270, 342, 356, 372, 395, 560
Sau3AI GATC 1 cut(s) 212
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 2 cut(s) 294, 314
SduI GDGCHC 1 cut(s) 34
SfaNI GCATC 3 cut(s) 9, 256, 298
SinI GGWCC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 3 cut(s) 254, 499, 627
SsiI CCGC 2 cut(s) 270, 602
SspMI CTAG 3 cut(s) 398, 527, 642
TaaI ACNGT 4 cut(s) 98, 494, 656, 663
TaiI ACGT 1 cut(s) 617
TaqI TCGA 2 cut(s) 57, 323
TaqII GACCGA 1 cut(s) 205
TasI AATT 3 cut(s) 254, 499, 627
TauI GCSGC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 175, 392
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 5 cut(s) 341, 355, 371, 394, 559
Tsp45I GTSAC 1 cut(s) 568
TspRI CASTG 2 cut(s) 175, 392
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 627
XceI RCATGY 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 3 cut(s) 398, 527, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.