Rh2BG113600

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005501
Physical Location & Seq
Reverse (-)
9633984 .. 9639834
5851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG113600.1

Sequence Viewer

Length: 2472 bp
ATGAAGAAATTGATCAATGAGGAAATATCCAAACGCTCAAGTACCAGGCACAATGGACCAAGCATTGTGGCACGGCTTATGGGGATGGATATGTTGCCAATGGATACAAAATCAGCAGTTCAGCCAATTGAGGACAAACATGAATATAGGAGAACGAAGTCTTCAAAGAAGGAAATCAATGGAAGGAGCTCAGTTGATCGTGTCTCTTCCAACTTAAATTCCTCCAGAGAGTTAGATCTTGATTCCTTTTATCAAAATAATGGCACAGATGATGCTGGATGGAGTGAAGATCAGAAAATTGGAAAACCAAGGCGTGAAGAACATCCTCAAGAGGAGGAACTACAGAAGTTCAAGAAAGAATTTGAAGCATGGCAAGCTGCCAGGTTTAGGGAATGTTCAAGGATTGTCGAAAATGACAACAATCAAGGTGAGTTGCTTGCTCAAGAGCATCTCAACAAGGAAAAGACGGCAGTTTCTGCAAAATCCAAGCAAACAACAATTGAGAAGACTATGGAAAGAATAGATCACACAGTAAAAGAAATCTCACATGAAAGAGGTGTTTCGTCACATCGTGTAGATACAATGGATTTATTCCCACCTGAGTACACAAGATCTTTATCTTCAAAAGGCAGAACCAAGAGCTTAGACTTTGAGCAATCCTCCCTGATGAATTCCAGGAAGCGAGTGAACATATCTTCTGCTCCTACAAGGATAGTTATCTTAAAACCTGGTCCTGATAGCTTTTGCAACCATGAAGAGACTTGGATCAATTCCCCAAGCACTTCAGAGCAGAGGGGTAGTATAGAAGATTTTCTTGAGGAGGTGAAGGATCGACTGAGATGTGAACTCCAAGGGAAGGTGCATAAAAGGGGTTCAGTTGTCCGAGGAAGTGGAATTGAGACCCCATTTAGTGAACAGCAATCGGATCCAAAGCAAATTGCTCGTCACATTGCAAACCAGGTCAGAGAAAGTGTGACTAGGGATCTTGGAGTGAATTTGCTTCGATCAGAATCAACAAGATCATACAGAAGTGAAATTCAATATAATGGAGCAGGTTCCCCAGAATTCATACATAGAGATACTAGAAGATTCCTGTTAGAGAGACTAAGAAATGTTTCAGAAAGAGAGACAGAACTTAATTCTCCTGTATTAGCTTCTGGCAGCTATAGGTCATCTGCACTAGACTATGAAAGGGTCAGATTAAAACAGGTGGGACATACTTTGGAAGCTCAAAAAGATATGAGTTTCTGGGGAAGAGGAATGGTGAAAGATGACCATGTAAAAACCAGATCATTCAGACATGGACCAGATGACGATAAGTTGCTCGATCGAGAGTTGTCCCCTAGAAATCTCATTAGGTCCTTATCTGCCCCAGTATCCGGAACATCATTTGGGAAGCTTCTTCTGGAGAACCGCCACATTTTGACTGGTGCTCACATTCAGAGGAAGCATGAAGCCATTGAACATGTGTCCTTGGATATGAAGAGTCAGAAGAAAGAAAGGTTTAATTTCAAAGAAAAGGTTTCCAATTTCAAATACAGTTTTACTCTTAGGGGCAGACTGTTTGGAAAAAGAATTCAATCAGCAACTGAATCACGTCGTACTGAACGTTACCCTATGAATGACATCAGGAGTGGCCCAACGGTTATTACCAACTCTAGAGAGAGACATGATAATTTCACCGAGGTGCCTCCTAGTCCTGCATCTGTGTGCAGCACTGCTCAGGACGATTTCTGCAGGACAGCTGATTGTCTTAGTCCAATATCAACTCCAAATGCAACTCCAAGAGAAGATAGACTTGTGCCACAGGCATTCAGAGACATCAGCGATAATCTAAGTGAACTGAGAAGGCAACTGAATCAACTCGAGTCTGATGAGCCGGATGATGCATCTGTTGAACAGGAAGTGATGGAATCTGAAATGTCTGGACTAGACAACCCGGCAGAGGTTTACATACGAGATTTGCTTGTTGCTTCTGGTCTGTACGATGGATCATTTGAGAAATCCTTTTCGAGATGGGACACGTTTGGGAAGCCCATCAGCCTCTCCGTGTTCAAAGAAGTGGAGGAATCTTATAAAAAGCTGGCCAATGATCACGATAGCTCAACCAAAGATCACAATGGGAAGGCAAATCACAAGTTGCTACTTGATTTGTTGAACGAAGCTCTTTCAACTGTTCTTGGACCTCCATTAAACATTTCTAAATTCAGAAGAAAAGCTATCAACGCCTCTGACCTGCCACCTTTACGTGGTAAAAAGCTGTTGGATTCTGTTTGGGAGAGTATATGTCATTATCTATACCCTCCAAACGGTAGACACTATCACTCCCTCGATGAAATAGTTTCCCGGGATCTGGGATCCTCACCGTGGTCGGAACTGGTGGAGGAAGACATTAATGTTCTCGGAAGAGAGATTGAAACTCTGATTATGAGAGATCTTGTTACAGAAGTTTTGGATGATGTGAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

823

Amino Acids

94.14

Weight (kDa)

6.72

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 650 - 816 2.3e-35 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2076
Acc36I ACCTGC 2 cut(s) 1043, 2244
AccB1I GGYRCC 1 cut(s) 1687
AccI GTMKAC 1 cut(s) 2314
AccIII TCCGGA 1 cut(s) 1379
AciI CCGC 1 cut(s) 1414
AclI AACGTT 1 cut(s) 1609
AclWI GGATC 9 cut(s) 773, 837, 920, 933, 990, 1999, 2352, 2358, 2365
AcoI YGGCCR 1 cut(s) 2085
AcsI RAATTY 8 cut(s) 217, 359, 670, 994, 1035, 1064, 1575, 2204
AcuI CTGAAG 1 cut(s) 768
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 4 cut(s) 43, 605, 1603, 1985
AfiI CCNNNNNNNGG 8 cut(s) 387, 856, 1379, 1945, 2249, 2309, 2353, 2367
AflIII ACRYGT 2 cut(s) 1465, 2022
AjiI CACGTC 1 cut(s) 1598
AjnI CCWGG 5 cut(s) 44, 380, 674, 727, 957
AleI CACNNNNGTG 1 cut(s) 1685
Alw21I GWGCWC 2 cut(s) 191, 1435
Alw26I GTCTC 7 cut(s) 208, 752, 893, 1096, 1121, 1660, 1812
AlwI GGATC 9 cut(s) 773, 837, 920, 933, 990, 1999, 2352, 2358, 2365
AlwNI CAGNNNCTG 2 cut(s) 476, 1589
Ama87I CYCGRG 2 cut(s) 1865, 2346
Aor13HI TCCGGA 1 cut(s) 1379
AoxI GGCC 2 cut(s) 1636, 2085
ApeKI GCWGC 3 cut(s) 377, 1161, 1713
ApoI RAATTY 8 cut(s) 217, 359, 670, 994, 1035, 1064, 1575, 2204
ArsI GACNNNNNNTTYG 2 cut(s) 928, 960
AseI ATTAAT 1 cut(s) 2394
Asp700I GAANNNNTTC 2 cut(s) 810, 1114
AspS9I GGNCC 6 cut(s) 56, 731, 1304, 1359, 1637, 2183
AsuC2I CCSGG 3 cut(s) 1940, 2347, 2348
AsuHPI GGTGA 5 cut(s) 440, 835, 1276, 1672, 2355
AvaI CYCGRG 2 cut(s) 1865, 2346
AvaII GGWCC 5 cut(s) 56, 731, 1304, 1359, 2183
BalI TGGCCA 1 cut(s) 2087
BamHI GGATCC 2 cut(s) 925, 2357
BanI GGYRCC 1 cut(s) 1687
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 3 cut(s) 153, 512, 2394
Bbv12I GWGCWC 2 cut(s) 191, 1435
BbvI GCAGC 3 cut(s) 364, 1173, 1725
BccI CCATC 6 cut(s) 79, 273, 1903, 1982, 2010, 2045
BceAI ACGGC 2 cut(s) 89, 483
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 5 cut(s) 46, 382, 676, 729, 959
BciVI GTATCC 2 cut(s) 97, 1387
BclI TGATCA 2 cut(s) 12, 2092
BcnI CCSGG 3 cut(s) 1940, 2347, 2348
BcoDI GTCTC 7 cut(s) 208, 752, 893, 1096, 1121, 1660, 1812
BfaI CTAG 7 cut(s) 978, 1083, 1181, 1344, 1659, 1695, 1931
BfmI CTRYAG 3 cut(s) 341, 1165, 1735
BfuAI ACCTGC 2 cut(s) 1043, 2244
BfuI GTATCC 2 cut(s) 97, 1387
BglII AGATCT 3 cut(s) 235, 611, 2434
BisI GCNGC 3 cut(s) 378, 1162, 1714
BlsI GCNGC 3 cut(s) 379, 1163, 1715
Bme1390I CCNGG 8 cut(s) 46, 382, 676, 729, 959, 1940, 2347, 2348
Bme18I GGWCC 5 cut(s) 56, 731, 1304, 1359, 2183
BmeT110I CYCGRG 2 cut(s) 1865, 2346
BmgBI CACGTC 1 cut(s) 1598
BmgT120I GGNCC 6 cut(s) 56, 731, 1304, 1359, 1637, 2183
BmiI GGNNCC 4 cut(s) 927, 1057, 1689, 2359
BmrFI CCNGG 8 cut(s) 46, 382, 676, 729, 959, 1940, 2347, 2348
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 5 cut(s) 262, 457, 1712, 1876, 1898
BmuI ACTGGG 1 cut(s) 1367
BpiI GAAGAC 3 cut(s) 153, 512, 2394
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 2 cut(s) 208, 1427
Bpu10I CCTNAGC 1 cut(s) 1722
BpuEI CTTGAG 4 cut(s) 22, 312, 426, 836
BpuMI CCSGG 3 cut(s) 1940, 2347, 2348
BsaAI YACGTR 1 cut(s) 2249
BsaBI GATNNNNATC 3 cut(s) 616, 716, 1009
BsaI GGTCTC 1 cut(s) 893
BsaJI CCNNGG 7 cut(s) 308, 850, 883, 1473, 1683, 2346, 2366
BsaWI WCCGGW 1 cut(s) 1379
BsaXI ACNNNNNCTCC 2 cut(s) 2309, 2339
Bsc4I CCNNNNNNNGG 8 cut(s) 387, 856, 1379, 1945, 2249, 2309, 2353, 2367
Bse1I ACTGG 3 cut(s) 1373, 1432, 2382
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 3 cut(s) 616, 716, 1009
BseAI TCCGGA 1 cut(s) 1379
BseBI CCWGG 5 cut(s) 46, 382, 676, 729, 959
BseDI CCNNGG 7 cut(s) 308, 850, 883, 1473, 1683, 2346, 2366
BseGI GGATG 5 cut(s) 90, 284, 322, 1888, 2461
BseJI GATNNNNATC 3 cut(s) 616, 716, 1009
BseLI CCNNNNNNNGG 8 cut(s) 387, 856, 1379, 1945, 2249, 2309, 2353, 2367
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 5 cut(s) 204, 591, 827, 1736, 1835
BseNI ACTGG 3 cut(s) 1373, 1432, 2382
BseRI GAGGAG 2 cut(s) 347, 833
BseXI GCAGC 3 cut(s) 364, 1173, 1725
BsgI GTGCAG 2 cut(s) 1161, 1732
Bsh1285I CGRYCG 1 cut(s) 1330
BshFI GGCC 2 cut(s) 1638, 2087
BshNI GGYRCC 1 cut(s) 1687
BsiEI CGRYCG 1 cut(s) 1330
BsiHKAI GWGCWC 2 cut(s) 191, 1435
BsiHKCI CYCGRG 2 cut(s) 1865, 2346
BsiSI CCGG 4 cut(s) 1380, 1880, 1940, 2347
BslFI GGGAC 3 cut(s) 1227, 1324, 2033
BslI CCNNNNNNNGG 8 cut(s) 387, 856, 1379, 1945, 2249, 2309, 2353, 2367
BsmAI GTCTC 7 cut(s) 208, 752, 893, 1096, 1121, 1660, 1812
BsmFI GGGAC 3 cut(s) 1227, 1324, 2033
BsmI GAATGC 1 cut(s) 1811
BsnI GGCC 2 cut(s) 1638, 2087
Bso31I GGTCTC 1 cut(s) 893
BsoBI CYCGRG 2 cut(s) 1865, 2346
Bsp1286I GDGCHC 2 cut(s) 191, 1435
Bsp13I TCCGGA 1 cut(s) 1379
BspACI CCGC 1 cut(s) 1414
BspANI GGCC 2 cut(s) 1638, 2087
BspCNI CTCAG 5 cut(s) 203, 592, 828, 1735, 1836
BspEI TCCGGA 1 cut(s) 1379
BspLI GGNNCC 4 cut(s) 927, 1057, 1689, 2359
BspMAI CTGCAG 1 cut(s) 1739
BspMI ACCTGC 2 cut(s) 1043, 2244
BspPI GGATC 9 cut(s) 773, 837, 920, 933, 990, 1999, 2352, 2358, 2365
BspT107I GGYRCC 1 cut(s) 1687
BspTNI GGTCTC 1 cut(s) 893
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 3 cut(s) 1373, 1432, 2382
BssECI CCNNGG 7 cut(s) 308, 850, 883, 1473, 1683, 2346, 2366
BssT1I CCWWGG 3 cut(s) 308, 850, 1473
Bst2UI CCWGG 5 cut(s) 46, 382, 676, 729, 959
Bst4CI ACNGT 7 cut(s) 532, 1541, 1563, 1645, 2176, 2312, 2367
Bst6I CTCTTC 5 cut(s) 211, 750, 1249, 1478, 2401
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 2249
BstC8I GCNNGC 3 cut(s) 375, 438, 2085
BstDSI CCRYGG 1 cut(s) 2366
BstF5I GGATG 5 cut(s) 90, 284, 322, 1888, 2461
BstMAI GTCTC 7 cut(s) 208, 752, 893, 1096, 1121, 1660, 1812
BstMCI CGRYCG 1 cut(s) 1330
BstMWI GCNNNNNNNGC 3 cut(s) 374, 476, 2225
BstNI CCWGG 5 cut(s) 46, 382, 676, 729, 959
BstNSI RCATGY 1 cut(s) 1469
BstSCI CCNGG 8 cut(s) 44, 380, 674, 727, 957, 1938, 2345, 2346
BstSFI CTRYAG 3 cut(s) 341, 1165, 1735
BstV1I GCAGC 3 cut(s) 364, 1173, 1725
BstV2I GAAGAC 3 cut(s) 153, 512, 2394
BstX2I RGATCY 7 cut(s) 235, 611, 925, 982, 2350, 2357, 2434
BstYI RGATCY 7 cut(s) 235, 611, 925, 982, 2350, 2357, 2434
BsuI GTATCC 2 cut(s) 97, 1387
BsuRI GGCC 2 cut(s) 1638, 2087
BtgI CCRYGG 1 cut(s) 2366
BtrI CACGTC 1 cut(s) 1598
BtsCI GGATG 5 cut(s) 90, 284, 322, 1888, 2461
BtsI GCAGTG 1 cut(s) 1716
BtsIMutI CAGTG 1 cut(s) 1716
BveI ACCTGC 2 cut(s) 1043, 2244
Cac8I GCNNGC 3 cut(s) 375, 438, 2085
CaiI CAGNNNCTG 2 cut(s) 476, 1589
Cfr13I GGNCC 6 cut(s) 56, 731, 1304, 1359, 1637, 2183
Cfr9I CCCGGG 1 cut(s) 2346
CsiI ACCWGGT 2 cut(s) 727, 957
Csp6I GTAC 4 cut(s) 42, 604, 1602, 1984
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 9 cut(s) 140, 369, 548, 752, 1277, 1301, 1451, 1466, 1670
CviQI GTAC 4 cut(s) 42, 604, 1602, 1984
DraIII CACNNNGTG 1 cut(s) 572
EaeI YGGCCR 1 cut(s) 2085
Eam1104I CTCTTC 5 cut(s) 211, 750, 1249, 1478, 2401
EarI CTCTTC 5 cut(s) 211, 750, 1249, 1478, 2401
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 3 cut(s) 308, 850, 1473
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 893
Eco47I GGWCC 5 cut(s) 56, 731, 1304, 1359, 2183
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 768
Eco88I CYCGRG 2 cut(s) 1865, 2346
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 1359
EcoRI GAATTC 3 cut(s) 670, 1064, 1575
EcoRII CCWGG 5 cut(s) 44, 380, 674, 727, 957
EcoT14I CCWWGG 3 cut(s) 308, 850, 1473
EcoT22I ATGCAT 1 cut(s) 1891
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 308, 850, 1473
FaeI CATG 9 cut(s) 143, 372, 551, 755, 1280, 1304, 1454, 1469, 1673
FalI AAGNNNNNCTT 4 cut(s) 798, 830, 1782, 1814
FaqI GGGAC 3 cut(s) 1227, 1324, 2033
FatI CATG 9 cut(s) 139, 368, 547, 751, 1276, 1300, 1450, 1465, 1669
FbaI TGATCA 2 cut(s) 12, 2092
FblI GTMKAC 1 cut(s) 2314
Fnu4HI GCNGC 3 cut(s) 378, 1162, 1714
FokI GGATG 5 cut(s) 97, 291, 309, 1895, 2468
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 378, 1162, 1714
FspBI CTAG 7 cut(s) 978, 1083, 1181, 1344, 1659, 1695, 1931
GluI GCNGC 3 cut(s) 378, 1162, 1714
GsuI CTGGAG 2 cut(s) 208, 1427
HaeIII GGCC 2 cut(s) 1638, 2087
HapII CCGG 4 cut(s) 1380, 1880, 1940, 2347
Hin1II CATG 9 cut(s) 143, 372, 551, 755, 1280, 1304, 1454, 1469, 1673
HindIII AAGCTT 1 cut(s) 1397
HpaII CCGG 4 cut(s) 1380, 1880, 1940, 2347
HphI GGTGA 5 cut(s) 440, 835, 1276, 1672, 2355
Hpy166II GTNNAC 7 cut(s) 606, 688, 845, 914, 1841, 1951, 2315
Hpy8I GTNNAC 7 cut(s) 606, 688, 845, 914, 1841, 1951, 2315
Hpy99I CGWCG 1 cut(s) 1602
HpyAV CCTTC 6 cut(s) 163, 177, 820, 850, 1842, 2119
HpyCH4III ACNGT 7 cut(s) 532, 1541, 1563, 1645, 2176, 2312, 2367
HpyCH4IV ACGT 4 cut(s) 1597, 1609, 2024, 2248
HpyF10VI GCNNNNNNNGC 3 cut(s) 374, 476, 2225
HpySE526I ACGT 4 cut(s) 1597, 1609, 2024, 2248
Hsp92II CATG 9 cut(s) 143, 372, 551, 755, 1280, 1304, 1454, 1469, 1673
Kpn2I TCCGGA 1 cut(s) 1379
Ksp22I TGATCA 2 cut(s) 12, 2092
LmnI GCTCC 3 cut(s) 186, 706, 1049
Lsp1109I GCAGC 3 cut(s) 364, 1173, 1725
LweI GCATC 5 cut(s) 262, 457, 1712, 1876, 1898
MabI ACCWGGT 2 cut(s) 727, 957
MaeI CTAG 7 cut(s) 978, 1083, 1181, 1344, 1659, 1695, 1931
MaeII ACGT 4 cut(s) 1597, 1609, 2024, 2248
MaeIII GTNAC 5 cut(s) 564, 944, 973, 1610, 2440
MfeI CAATTG 2 cut(s) 126, 498
MflI RGATCY 7 cut(s) 235, 611, 925, 982, 2350, 2357, 2434
MhlI GDGCHC 2 cut(s) 191, 1435
MlsI TGGCCA 1 cut(s) 2087
MluNI TGGCCA 1 cut(s) 2087
MlyI GAGTC 2 cut(s) 1495, 1877
MmeI TCCRAC 3 cut(s) 234, 2244, 2352
Mox20I TGGCCA 1 cut(s) 2087
Mph1103I ATGCAT 1 cut(s) 1891
MroI TCCGGA 1 cut(s) 1379
MroXI GAANNNNTTC 2 cut(s) 810, 1114
MscI TGGCCA 1 cut(s) 2087
MseI TTAA 7 cut(s) 215, 722, 1137, 1202, 1506, 2192, 2394
MslI CAYNNNNRTG 2 cut(s) 1685, 1708
Msp20I TGGCCA 1 cut(s) 2087
MspA1I CMGCKG 1 cut(s) 1745
MspI CCGG 4 cut(s) 1380, 1880, 1940, 2347
MspR9I CCNGG 8 cut(s) 46, 382, 676, 729, 959, 1940, 2347, 2348
MunI CAATTG 2 cut(s) 126, 498
Mva1269I GAATGC 1 cut(s) 1811
MvaI CCWGG 5 cut(s) 46, 382, 676, 729, 959
MwoI GCNNNNNNNGC 3 cut(s) 374, 476, 2225
NciI CCSGG 3 cut(s) 1940, 2347, 2348
NlaIII CATG 9 cut(s) 143, 372, 551, 755, 1280, 1304, 1454, 1469, 1673
NlaIV GGNNCC 4 cut(s) 927, 1057, 1689, 2359
NmuCI GTSAC 3 cut(s) 564, 944, 973
NsiI ATGCAT 1 cut(s) 1891
NspI RCATGY 1 cut(s) 1469
OliI CACNNNNGTG 1 cut(s) 1685
PaeR7I CTCGAG 1 cut(s) 1865
PciI ACATGT 1 cut(s) 1465
PcsI WCGNNNNNNNCGW 1 cut(s) 1606
PctI GAATGC 1 cut(s) 1811
PdmI GAANNNNTTC 2 cut(s) 810, 1114
PfeI GAWTC 8 cut(s) 242, 1010, 1089, 1592, 1858, 1913, 2069, 2267
PfoI TCCNGGA 1 cut(s) 674
PkrI GCNGC 3 cut(s) 379, 1163, 1715
Ple19I CGATCG 1 cut(s) 1330
PleI GAGTC 2 cut(s) 1494, 1876
PpsI GAGTC 2 cut(s) 1494, 1876
Ppu21I YACGTR 1 cut(s) 2249
PpuMI RGGWCCY 1 cut(s) 1359
PscI ACATGT 1 cut(s) 1465
PshBI ATTAAT 1 cut(s) 2394
PsiI TTATAA 1 cut(s) 2076
Psp124BI GAGCTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 1609
Psp5II RGGWCCY 1 cut(s) 1359
Psp6I CCWGG 5 cut(s) 44, 380, 674, 727, 957
PspGI CCWGG 5 cut(s) 44, 380, 674, 727, 957
PspN4I GGNNCC 4 cut(s) 927, 1057, 1689, 2359
PspPI GGNCC 6 cut(s) 56, 731, 1304, 1359, 1637, 2183
PspPPI RGGWCCY 1 cut(s) 1359
PspXI VCTCGAGB 1 cut(s) 1865
PstI CTGCAG 1 cut(s) 1739
PstNI CAGNNNCTG 2 cut(s) 476, 1589
PsuI RGATCY 7 cut(s) 235, 611, 925, 982, 2350, 2357, 2434
PvuI CGATCG 1 cut(s) 1330
PvuII CAGCTG 1 cut(s) 1745
RsaI GTAC 4 cut(s) 43, 605, 1603, 1985
RsaNI GTAC 4 cut(s) 42, 604, 1602, 1984
RseI CAYNNNNRTG 2 cut(s) 1685, 1708
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 7 cut(s) 215, 722, 1137, 1202, 1506, 2192, 2394
SatI GCNGC 3 cut(s) 378, 1162, 1714
Sau96I GGNCC 6 cut(s) 56, 731, 1304, 1359, 1637, 2183
SchI GAGTC 2 cut(s) 1495, 1877
ScrFI CCNGG 8 cut(s) 46, 382, 676, 729, 959, 1940, 2347, 2348
SduI GDGCHC 2 cut(s) 191, 1435
SexAI ACCWGGT 2 cut(s) 727, 957
SfaNI GCATC 5 cut(s) 262, 457, 1712, 1876, 1898
SfcI CTRYAG 3 cut(s) 341, 1165, 1735
Sfr274I CTCGAG 1 cut(s) 1865
SinI GGWCC 5 cut(s) 56, 731, 1304, 1359, 2183
SlaI CTCGAG 1 cut(s) 1865
SmaI CCCGGG 1 cut(s) 2348
SmiMI CAYNNNNRTG 2 cut(s) 1685, 1708
SmlI CTYRAG 5 cut(s) 37, 327, 441, 815, 1865
SmoI CTYRAG 5 cut(s) 37, 327, 441, 815, 1865
SsiI CCGC 1 cut(s) 1414
SspMI CTAG 7 cut(s) 978, 1083, 1181, 1344, 1659, 1695, 1931
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 8 cut(s) 44, 380, 674, 727, 957, 1938, 2345, 2346
StyI CCWWGG 3 cut(s) 308, 850, 1473
TaaI ACNGT 7 cut(s) 532, 1541, 1563, 1645, 2176, 2312, 2367
TaiI ACGT 4 cut(s) 1600, 1612, 2027, 2251
TaqI TCGA 8 cut(s) 408, 832, 1003, 1326, 1330, 1866, 2012, 2331
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 8 cut(s) 242, 1010, 1089, 1592, 1858, 1913, 2069, 2267
Tru1I TTAA 7 cut(s) 215, 722, 1137, 1202, 1506, 2192, 2394
Tru9I TTAA 7 cut(s) 215, 722, 1137, 1202, 1506, 2192, 2394
TscAI CASTG 1 cut(s) 1723
TseFI GTSAC 3 cut(s) 564, 944, 973
TseI GCWGC 3 cut(s) 377, 1161, 1713
Tsp45I GTSAC 3 cut(s) 564, 944, 973
TspGWI ACGGA 1 cut(s) 2038
TspMI CCCGGG 1 cut(s) 2346
TspRI CASTG 1 cut(s) 1723
VpaK11BI GGWCC 5 cut(s) 56, 731, 1304, 1359, 2183
VspI ATTAAT 1 cut(s) 2394
XapI RAATTY 8 cut(s) 217, 359, 670, 994, 1035, 1064, 1575, 2204
XbaI TCTAGA 1 cut(s) 1658
XceI RCATGY 1 cut(s) 1469
XcmI CCANNNNNNNNNTGG 1 cut(s) 1424
XhoI CTCGAG 1 cut(s) 1865
XmaI CCCGGG 1 cut(s) 2346
XmiI GTMKAC 1 cut(s) 2314
XmnI GAANNNNTTC 2 cut(s) 810, 1114
XspI CTAG 7 cut(s) 978, 1083, 1181, 1344, 1659, 1695, 1931
Zsp2I ATGCAT 1 cut(s) 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.