Rw2G008680

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005501
Physical Location & Seq
Reverse (-)
8705422 .. 8710995
5574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008680.1

Sequence Viewer

Length: 2676 bp
ATGGGAGGCTTATTGCACCTTTTTGATTTCAATCAGGGCAGCATGGCCAGGAAGCTTTTTCTGCATAAGAAACATGATGGTGGCCTTGATGCTCCACGAAATAGCTTGGAGCTGCAAATAGAAACTTCTCAGAGCTATAATGATGTGGGAGATTTGCCGGTGAAAGAAGTTGGTCCCAAAAAGAATTATCCACTTCAAAGTTCAATGAAGAAATTGATCAATGAGGAAATATCCAAACGCTCAAGTACCAGGCACAATGGACCAAGCATTGTGGCACGGCTTATGGGGATGGATATGTTGCCAATGGATACAAAATCAGCAGTTCAGCCAATTGAGGACAAACATGAATATAGGAGAACGAAGTCTTCAAAGAAGGAAATCAATGGAAGGAGCTCAGTTGATCGTGTCTCTTCCAACTTAAATTCCTCCAGAGAGTTAGATCTTGATTCCTGTTATCAAAATAATGGCACAGATGATGCTGGATGGAGTGAAGATCAGAAAATTGGAAAACCAAGGCGTGAAGAACATCCTCAAGAGGAGGAACTACAGAAGTTCAAGAAAGAATTTGAAGCATGGCAAGCTGCCAGGTTTAGGGAATGTTCAAGGATTGTCGAAAATGACAACAATCAAGGTGAGTTGCTTGCTCAAGAGCACCTCAACAAGGAAAAGACGGCAGTTTCTGCAAAATCCAAGCAAACAACAATTGAGAAGACTATGGAAAGAATAGATCACACAGTAAAAGAAATCTCACATGAAAGAGGTGTTTCGTCACATCGTGTAGATACAATGGATTTATTCCCACCTGAGTACACAAGATCTTTATCTTCAAAAGGCAGAACCAAGAGCTTAGACTTTGAGCAATCCTCCCTGATGAATTCCAGGAAGCGAGTGAACATATCTTCTGCTCCTACAAGGATAGTTATCTTAAAACCTGGTCCTGATAGCTTTTGCAACCATGAAGAGACTTGGATCAATTCCCCAAGCACTTCAGAGCAGAGGGGTAGTATAGAAGATTTTCTTGAGGAGGTGAAGGATCGACTGAGATGTGAACTCCAAGGGAAGGTGCATAAAAGGGGTTCAGTTGTCCGAGGAAGTGGAATTGAGACCCCATTTAGTGAACAGCCATCGGATCCAAAGCAAATTGCTCGTCACATTGCAAACCAGGTCAGAGAAAGTGTGACTAGGGATCTTGGAGTGAATTTGCTTCGATCAGAATCAACAAGATCATACAGAAGTGAAATTCAATATAATGGAGCAGGTTCTCCAGAATTCATACATAGAGATACTAGAAGATTCCTGTTAGAGAGACTAAGAAATGTTTCAGAAAGAGAGACAGAACTTAATTCTCCTGTATTAACTTCTGGCAGCTATAGGTCATCTGCACTAGACTATGAAAGGGTCAGATTAAAACAGGTGGGACATACTTTGGAAGCTCAAAAAGATATGAGTTTCTGGGAAAGAGGAATGGTGAAAGATGACCATGTAAAAACCAGATCATTCAGGCATGGACCAGACGACGATAAGTTGCTCGATCGAGAGTTGTCCCCTAGAAATCTCATTAGGTCCTTATCTGCCCCAGTATCCGGAACATCATTTGGGAAGCTTCTTCTGGAGAACCGCCACATTTTGACTGGTGCTCACATTCAGAGGAAGCATGAAGCCATTGAACATGTGTCCTTGGATATGAAGAGTCGGAAGAAAGAAAGGTTTAATTTCAAAGAAAAGGTTTCCAATTTCAAATACAGTTTTACTCTTAGGGGCAGACTGTTTGGAAAAAGAATTCAATCAGCAACTGAATCACGTCGTACTGAACGTTACCCTATGAATGACATCAGGAGTGGCCCAACAGTTATTACCAACTCTGGAGAGAGACATGATAATTTCACCGAGGTGCCTCCTAGTCCTGCATCTGTGTGCAGCACTGCTCAGGACGATTTCTGCAGGACAGCTGATTGTCTTAGTCCAATATCAACTCCAAATGCAACTCCAAGAGAAGATAGACTTGTGCCACAGGCATTCAGAGACATCAGCGATAATCTAAGTGAACTGAGAAGGCAATTGAATCAACTCGAGTCTGATGAGCCGGATGATGCATCTGTTGAACAGGAAGTGATGGAATCTGAAATGTCTGGACTAGACAACCCGGCAGAGGTTTACATACGAGATTTGCTTGTTGCTTCTGGTCTGTACGATGGATCATTTGAGAAATCCTTTTCGAGATGGGACACGTTTGGGAAGCCCATCAGCCTCTCCGTGTTCAAAGAAGTGGAGGAATCTTATAAAAAGCTGGCCAATGATCACGATAGCTCAACCAAAGATCACAATGGGAAGGCAAATCACAAGTTGCTACTTGATTTGTTGAACGAAGCTCTTTCAACTGTTCTTGGACCTCCATTAAACATTTCTAAATTCAGAAGAAAAGCTATCAACGCCTCTGATCTGCCACCTTTACGTGGTAAGAAGCTGTTGGATTCTGTTTGGGAGAGTATATGTCATTATCTATACCCTCCAAACGGTAGACACTATCACTCCCTCGATGAAATAGTTTCCCGGGATCTGGGATCCTCACTGTGGTCGGAACTGGTGGAGGAAGACATTAATGTTCTCGGAAGAGAGATTGAAACTCTGATTATGAGAGATCTTGTTACAGAAGTTTTGGATGATGTGAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

891

Amino Acids

101.66

Weight (kDa)

6.67

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 718 - 884 9e-35 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2280
Acc36I ACCTGC 1 cut(s) 1247
AccB1I GGYRCC 1 cut(s) 1891
AccI GTMKAC 1 cut(s) 2518
AccIII TCCGGA 1 cut(s) 1583
AciI CCGC 1 cut(s) 1618
AclI AACGTT 1 cut(s) 1813
AclWI GGATC 9 cut(s) 977, 1041, 1124, 1137, 1194, 2203, 2556, 2562, 2569
AcoI YGGCCR 2 cut(s) 45, 2289
AcsI RAATTY 8 cut(s) 421, 563, 874, 1198, 1239, 1268, 1779, 2408
AcuI CTGAAG 1 cut(s) 972
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 4 cut(s) 247, 809, 1807, 2189
AfiI CCNNNNNNNGG 9 cut(s) 591, 661, 1060, 1583, 2149, 2453, 2513, 2557, 2571
AflIII ACRYGT 2 cut(s) 1669, 2226
AjiI CACGTC 1 cut(s) 1802
AjnI CCWGG 6 cut(s) 47, 248, 584, 878, 931, 1161
AleI CACNNNNGTG 1 cut(s) 1889
Alw21I GWGCWC 3 cut(s) 395, 654, 1639
Alw26I GTCTC 7 cut(s) 412, 956, 1097, 1300, 1325, 1864, 2016
AlwI GGATC 9 cut(s) 977, 1041, 1124, 1137, 1194, 2203, 2556, 2562, 2569
AlwNI CAGNNNCTG 2 cut(s) 680, 1793
Ama87I CYCGRG 2 cut(s) 2069, 2550
Aor13HI TCCGGA 1 cut(s) 1583
AoxI GGCC 4 cut(s) 45, 82, 1840, 2289
ApeKI GCWGC 5 cut(s) 39, 112, 581, 1365, 1917
ApoI RAATTY 8 cut(s) 421, 563, 874, 1198, 1239, 1268, 1779, 2408
ArsI GACNNNNNNTTYG 2 cut(s) 1132, 1164
AseI ATTAAT 1 cut(s) 2598
Asp700I GAANNNNTTC 2 cut(s) 1014, 1318
AspS9I GGNCC 7 cut(s) 173, 260, 935, 1508, 1563, 1841, 2387
AsuC2I CCSGG 3 cut(s) 2144, 2551, 2552
AsuHPI GGTGA 5 cut(s) 172, 644, 1039, 1480, 1876
AvaI CYCGRG 2 cut(s) 2069, 2550
AvaII GGWCC 6 cut(s) 173, 260, 935, 1508, 1563, 2387
BalI TGGCCA 2 cut(s) 47, 2291
BamHI GGATCC 2 cut(s) 1129, 2561
BanI GGYRCC 1 cut(s) 1891
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 3 cut(s) 357, 716, 2598
Bbv12I GWGCWC 3 cut(s) 395, 654, 1639
BbvI GCAGC 5 cut(s) 51, 99, 568, 1377, 1929
BccI CCATC 8 cut(s) 71, 283, 477, 1132, 2107, 2186, 2214, 2249
BceAI ACGGC 2 cut(s) 293, 687
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 6 cut(s) 49, 250, 586, 880, 933, 1163
BciVI GTATCC 2 cut(s) 301, 1591
BclI TGATCA 2 cut(s) 216, 2296
BcnI CCSGG 3 cut(s) 2144, 2551, 2552
BcoDI GTCTC 7 cut(s) 412, 956, 1097, 1300, 1325, 1864, 2016
BfaI CTAG 6 cut(s) 1182, 1287, 1385, 1548, 1899, 2135
BfmI CTRYAG 3 cut(s) 545, 1369, 1939
BfuAI ACCTGC 1 cut(s) 1247
BfuI GTATCC 2 cut(s) 301, 1591
BglII AGATCT 3 cut(s) 439, 815, 2638
BisI GCNGC 5 cut(s) 40, 113, 582, 1366, 1918
BlsI GCNGC 5 cut(s) 41, 114, 583, 1367, 1919
Bme1390I CCNGG 9 cut(s) 49, 250, 586, 880, 933, 1163, 2144, 2551, 2552
Bme18I GGWCC 6 cut(s) 173, 260, 935, 1508, 1563, 2387
BmeT110I CYCGRG 2 cut(s) 2069, 2550
BmgBI CACGTC 1 cut(s) 1802
BmgT120I GGNCC 7 cut(s) 173, 260, 935, 1508, 1563, 1841, 2387
BmiI GGNNCC 4 cut(s) 175, 1131, 1893, 2563
BmrFI CCNGG 9 cut(s) 49, 250, 586, 880, 933, 1163, 2144, 2551, 2552
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 5 cut(s) 79, 466, 1916, 2080, 2102
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 3 cut(s) 357, 716, 2598
BplI GAGNNNNNCTC 2 cut(s) 848, 880
BpmI CTGGAG 4 cut(s) 412, 1248, 1631, 1884
Bpu10I CCTNAGC 1 cut(s) 1926
BpuEI CTTGAG 4 cut(s) 226, 516, 630, 1040
BpuMI CCSGG 3 cut(s) 2144, 2551, 2552
BsaAI YACGTR 1 cut(s) 2453
BsaBI GATNNNNATC 4 cut(s) 30, 820, 920, 1213
BsaI GGTCTC 1 cut(s) 1097
BsaJI CCNNGG 6 cut(s) 512, 1054, 1087, 1677, 1887, 2550
BsaWI WCCGGW 1 cut(s) 1583
BsaXI ACNNNNNCTCC 2 cut(s) 2513, 2543
Bsc4I CCNNNNNNNGG 9 cut(s) 591, 661, 1060, 1583, 2149, 2453, 2513, 2557, 2571
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 3 cut(s) 1577, 1636, 2586
Bse3DI GCAATG 1 cut(s) 1152
Bse8I GATNNNNATC 4 cut(s) 30, 820, 920, 1213
BseAI TCCGGA 1 cut(s) 1583
BseBI CCWGG 6 cut(s) 49, 250, 586, 880, 933, 1163
BseDI CCNNGG 6 cut(s) 512, 1054, 1087, 1677, 1887, 2550
BseGI GGATG 5 cut(s) 294, 488, 526, 2092, 2665
BseJI GATNNNNATC 4 cut(s) 30, 820, 920, 1213
BseLI CCNNNNNNNGG 9 cut(s) 591, 661, 1060, 1583, 2149, 2453, 2513, 2557, 2571
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 6 cut(s) 143, 408, 795, 1031, 1940, 2039
BseNI ACTGG 3 cut(s) 1577, 1636, 2586
BseRI GAGGAG 2 cut(s) 551, 1037
BseXI GCAGC 5 cut(s) 51, 99, 568, 1377, 1929
BsgI GTGCAG 2 cut(s) 1365, 1936
Bsh1285I CGRYCG 1 cut(s) 1534
BshFI GGCC 4 cut(s) 47, 84, 1842, 2291
BshNI GGYRCC 1 cut(s) 1891
BsiEI CGRYCG 1 cut(s) 1534
BsiHKAI GWGCWC 3 cut(s) 395, 654, 1639
BsiHKCI CYCGRG 2 cut(s) 2069, 2550
BsiSI CCGG 5 cut(s) 158, 1584, 2084, 2144, 2551
BslFI GGGAC 4 cut(s) 159, 1431, 1528, 2237
BslI CCNNNNNNNGG 9 cut(s) 591, 661, 1060, 1583, 2149, 2453, 2513, 2557, 2571
BsmAI GTCTC 7 cut(s) 412, 956, 1097, 1300, 1325, 1864, 2016
BsmFI GGGAC 4 cut(s) 159, 1431, 1528, 2237
BsmI GAATGC 1 cut(s) 2015
BsnI GGCC 4 cut(s) 47, 84, 1842, 2291
Bso31I GGTCTC 1 cut(s) 1097
BsoBI CYCGRG 2 cut(s) 2069, 2550
Bsp1286I GDGCHC 3 cut(s) 395, 654, 1639
Bsp13I TCCGGA 1 cut(s) 1583
BspACI CCGC 1 cut(s) 1618
BspANI GGCC 4 cut(s) 47, 84, 1842, 2291
BspCNI CTCAG 6 cut(s) 142, 407, 796, 1032, 1939, 2040
BspEI TCCGGA 1 cut(s) 1583
BspLI GGNNCC 4 cut(s) 175, 1131, 1893, 2563
BspMAI CTGCAG 1 cut(s) 1943
BspMI ACCTGC 1 cut(s) 1247
BspPI GGATC 9 cut(s) 977, 1041, 1124, 1137, 1194, 2203, 2556, 2562, 2569
BspT107I GGYRCC 1 cut(s) 1891
BspTNI GGTCTC 1 cut(s) 1097
BsrDI GCAATG 1 cut(s) 1152
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 3 cut(s) 1577, 1636, 2586
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 6 cut(s) 512, 1054, 1087, 1677, 1887, 2550
BssT1I CCWWGG 3 cut(s) 512, 1054, 1677
Bst2UI CCWGG 6 cut(s) 49, 250, 586, 880, 933, 1163
Bst4CI ACNGT 7 cut(s) 736, 1745, 1767, 1849, 2380, 2516, 2571
Bst6I CTCTTC 4 cut(s) 415, 954, 1682, 2605
BstAPI GCANNNNNTGC 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 2453
BstC8I GCNNGC 3 cut(s) 579, 642, 2289
BstENI CCTNNNNNAGG 1 cut(s) 659
BstF5I GGATG 5 cut(s) 294, 488, 526, 2092, 2665
BstMAI GTCTC 7 cut(s) 412, 956, 1097, 1300, 1325, 1864, 2016
BstMCI CGRYCG 1 cut(s) 1534
BstMWI GCNNNNNNNGC 4 cut(s) 61, 578, 680, 2429
BstNI CCWGG 6 cut(s) 49, 250, 586, 880, 933, 1163
BstNSI RCATGY 1 cut(s) 1673
BstSCI CCNGG 9 cut(s) 47, 248, 584, 878, 931, 1161, 2142, 2549, 2550
BstSFI CTRYAG 3 cut(s) 545, 1369, 1939
BstV1I GCAGC 5 cut(s) 51, 99, 568, 1377, 1929
BstV2I GAAGAC 3 cut(s) 357, 716, 2598
BstX2I RGATCY 7 cut(s) 439, 815, 1129, 1186, 2554, 2561, 2638
BstYI RGATCY 7 cut(s) 439, 815, 1129, 1186, 2554, 2561, 2638
BsuI GTATCC 2 cut(s) 301, 1591
BsuRI GGCC 4 cut(s) 47, 84, 1842, 2291
BtrI CACGTC 1 cut(s) 1802
BtsCI GGATG 5 cut(s) 294, 488, 526, 2092, 2665
BtsI GCAGTG 1 cut(s) 1920
BtsIMutI CAGTG 2 cut(s) 1920, 2567
BveI ACCTGC 1 cut(s) 1247
Cac8I GCNNGC 3 cut(s) 579, 642, 2289
CaiI CAGNNNCTG 2 cut(s) 680, 1793
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 7 cut(s) 173, 260, 935, 1508, 1563, 1841, 2387
Cfr9I CCCGGG 1 cut(s) 2550
CsiI ACCWGGT 2 cut(s) 931, 1161
Csp6I GTAC 4 cut(s) 246, 808, 1806, 2188
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviQI GTAC 4 cut(s) 246, 808, 1806, 2188
DraIII CACNNNGTG 1 cut(s) 776
EaeI YGGCCR 2 cut(s) 45, 2289
Eam1104I CTCTTC 4 cut(s) 415, 954, 1682, 2605
EarI CTCTTC 4 cut(s) 415, 954, 1682, 2605
Ecl136II GAGCTC 1 cut(s) 393
Eco130I CCWWGG 3 cut(s) 512, 1054, 1677
Eco24I GRGCYC 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 1097
Eco47I GGWCC 6 cut(s) 173, 260, 935, 1508, 1563, 2387
Eco53kI GAGCTC 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 972
Eco88I CYCGRG 2 cut(s) 2069, 2550
EcoICRI GAGCTC 1 cut(s) 393
EcoNI CCTNNNNNAGG 1 cut(s) 659
EcoO109I RGGNCCY 1 cut(s) 1563
EcoRI GAATTC 3 cut(s) 874, 1268, 1779
EcoRII CCWGG 6 cut(s) 47, 248, 584, 878, 931, 1161
EcoT14I CCWWGG 3 cut(s) 512, 1054, 1677
EcoT22I ATGCAT 1 cut(s) 2095
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 3 cut(s) 512, 1054, 1677
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 1986, 2018
FaqI GGGAC 4 cut(s) 159, 1431, 1528, 2237
FbaI TGATCA 2 cut(s) 216, 2296
FblI GTMKAC 1 cut(s) 2518
Fnu4HI GCNGC 5 cut(s) 40, 113, 582, 1366, 1918
FokI GGATG 5 cut(s) 301, 495, 513, 2099, 2672
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 5 cut(s) 40, 113, 582, 1366, 1918
FspBI CTAG 6 cut(s) 1182, 1287, 1385, 1548, 1899, 2135
GluI GCNGC 5 cut(s) 40, 113, 582, 1366, 1918
GsuI CTGGAG 4 cut(s) 412, 1248, 1631, 1884
HaeIII GGCC 4 cut(s) 47, 84, 1842, 2291
HapII CCGG 5 cut(s) 158, 1584, 2084, 2144, 2551
HindIII AAGCTT 2 cut(s) 53, 1601
HpaII CCGG 5 cut(s) 158, 1584, 2084, 2144, 2551
HphI GGTGA 5 cut(s) 172, 644, 1039, 1480, 1876
Hpy166II GTNNAC 7 cut(s) 810, 892, 1049, 1118, 2045, 2155, 2519
Hpy8I GTNNAC 7 cut(s) 810, 892, 1049, 1118, 2045, 2155, 2519
Hpy99I CGWCG 2 cut(s) 1520, 1806
HpyAV CCTTC 6 cut(s) 367, 381, 1024, 1054, 2046, 2323
HpyCH4III ACNGT 7 cut(s) 736, 1745, 1767, 1849, 2380, 2516, 2571
HpyCH4IV ACGT 4 cut(s) 1801, 1813, 2228, 2452
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 578, 680, 2429
HpySE526I ACGT 4 cut(s) 1801, 1813, 2228, 2452
Kpn2I TCCGGA 1 cut(s) 1583
Ksp22I TGATCA 2 cut(s) 216, 2296
LmnI GCTCC 5 cut(s) 97, 109, 390, 910, 1253
Lsp1109I GCAGC 5 cut(s) 51, 99, 568, 1377, 1929
LweI GCATC 5 cut(s) 79, 466, 1916, 2080, 2102
MabI ACCWGGT 2 cut(s) 931, 1161
MaeI CTAG 6 cut(s) 1182, 1287, 1385, 1548, 1899, 2135
MaeII ACGT 4 cut(s) 1801, 1813, 2228, 2452
MaeIII GTNAC 5 cut(s) 768, 1148, 1177, 1814, 2644
MfeI CAATTG 3 cut(s) 330, 702, 2057
MflI RGATCY 7 cut(s) 439, 815, 1129, 1186, 2554, 2561, 2638
MhlI GDGCHC 3 cut(s) 395, 654, 1639
MlsI TGGCCA 2 cut(s) 47, 2291
MluNI TGGCCA 2 cut(s) 47, 2291
MlyI GAGTC 2 cut(s) 1699, 2081
MmeI TCCRAC 4 cut(s) 438, 1673, 2448, 2556
Mox20I TGGCCA 2 cut(s) 47, 2291
Mph1103I ATGCAT 1 cut(s) 2095
MroI TCCGGA 1 cut(s) 1583
MroXI GAANNNNTTC 2 cut(s) 1014, 1318
MscI TGGCCA 2 cut(s) 47, 2291
MseI TTAA 8 cut(s) 419, 926, 1341, 1355, 1406, 1710, 2396, 2598
MslI CAYNNNNRTG 3 cut(s) 78, 1889, 1912
Msp20I TGGCCA 2 cut(s) 47, 2291
MspA1I CMGCKG 1 cut(s) 1949
MspI CCGG 5 cut(s) 158, 1584, 2084, 2144, 2551
MspR9I CCNGG 9 cut(s) 49, 250, 586, 880, 933, 1163, 2144, 2551, 2552
MunI CAATTG 3 cut(s) 330, 702, 2057
Mva1269I GAATGC 1 cut(s) 2015
MvaI CCWGG 6 cut(s) 49, 250, 586, 880, 933, 1163
MwoI GCNNNNNNNGC 4 cut(s) 61, 578, 680, 2429
NciI CCSGG 3 cut(s) 2144, 2551, 2552
NlaIV GGNNCC 4 cut(s) 175, 1131, 1893, 2563
NmuCI GTSAC 3 cut(s) 768, 1148, 1177
NsiI ATGCAT 1 cut(s) 2095
NspI RCATGY 1 cut(s) 1673
OliI CACNNNNGTG 1 cut(s) 1889
PaeR7I CTCGAG 1 cut(s) 2069
PciI ACATGT 1 cut(s) 1669
PcsI WCGNNNNNNNCGW 1 cut(s) 1810
PctI GAATGC 1 cut(s) 2015
PdmI GAANNNNTTC 2 cut(s) 1014, 1318
PfeI GAWTC 8 cut(s) 446, 1214, 1293, 1796, 2062, 2117, 2273, 2471
PfoI TCCNGGA 1 cut(s) 878
PkrI GCNGC 5 cut(s) 41, 114, 583, 1367, 1919
Ple19I CGATCG 1 cut(s) 1534
PleI GAGTC 2 cut(s) 1698, 2080
PpsI GAGTC 2 cut(s) 1698, 2080
Ppu21I YACGTR 1 cut(s) 2453
PpuMI RGGWCCY 1 cut(s) 1563
PscI ACATGT 1 cut(s) 1669
PshBI ATTAAT 1 cut(s) 2598
PsiI TTATAA 1 cut(s) 2280
Psp124BI GAGCTC 1 cut(s) 395
Psp1406I AACGTT 1 cut(s) 1813
Psp5II RGGWCCY 1 cut(s) 1563
Psp6I CCWGG 6 cut(s) 47, 248, 584, 878, 931, 1161
PspGI CCWGG 6 cut(s) 47, 248, 584, 878, 931, 1161
PspN4I GGNNCC 4 cut(s) 175, 1131, 1893, 2563
PspPI GGNCC 7 cut(s) 173, 260, 935, 1508, 1563, 1841, 2387
PspPPI RGGWCCY 1 cut(s) 1563
PspXI VCTCGAGB 1 cut(s) 2069
PstI CTGCAG 1 cut(s) 1943
PstNI CAGNNNCTG 2 cut(s) 680, 1793
PsuI RGATCY 7 cut(s) 439, 815, 1129, 1186, 2554, 2561, 2638
PvuI CGATCG 1 cut(s) 1534
PvuII CAGCTG 1 cut(s) 1949
RsaI GTAC 4 cut(s) 247, 809, 1807, 2189
RsaNI GTAC 4 cut(s) 246, 808, 1806, 2188
RseI CAYNNNNRTG 3 cut(s) 78, 1889, 1912
SacI GAGCTC 1 cut(s) 395
SaqAI TTAA 8 cut(s) 419, 926, 1341, 1355, 1406, 1710, 2396, 2598
SatI GCNGC 5 cut(s) 40, 113, 582, 1366, 1918
Sau96I GGNCC 7 cut(s) 173, 260, 935, 1508, 1563, 1841, 2387
SchI GAGTC 2 cut(s) 1699, 2081
ScrFI CCNGG 9 cut(s) 49, 250, 586, 880, 933, 1163, 2144, 2551, 2552
SduI GDGCHC 3 cut(s) 395, 654, 1639
SexAI ACCWGGT 2 cut(s) 931, 1161
SfaNI GCATC 5 cut(s) 79, 466, 1916, 2080, 2102
SfcI CTRYAG 3 cut(s) 545, 1369, 1939
Sfr274I CTCGAG 1 cut(s) 2069
SinI GGWCC 6 cut(s) 173, 260, 935, 1508, 1563, 2387
SlaI CTCGAG 1 cut(s) 2069
SmaI CCCGGG 1 cut(s) 2552
SmiMI CAYNNNNRTG 3 cut(s) 78, 1889, 1912
SmlI CTYRAG 5 cut(s) 241, 531, 645, 1019, 2069
SmoI CTYRAG 5 cut(s) 241, 531, 645, 1019, 2069
SsiI CCGC 1 cut(s) 1618
SspMI CTAG 6 cut(s) 1182, 1287, 1385, 1548, 1899, 2135
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 9 cut(s) 47, 248, 584, 878, 931, 1161, 2142, 2549, 2550
StyI CCWWGG 3 cut(s) 512, 1054, 1677
TaaI ACNGT 7 cut(s) 736, 1745, 1767, 1849, 2380, 2516, 2571
TaiI ACGT 4 cut(s) 1804, 1816, 2231, 2455
TaqI TCGA 8 cut(s) 612, 1036, 1207, 1530, 1534, 2070, 2216, 2535
TatI WGTACW 1 cut(s) 807
TfiI GAWTC 8 cut(s) 446, 1214, 1293, 1796, 2062, 2117, 2273, 2471
Tru1I TTAA 8 cut(s) 419, 926, 1341, 1355, 1406, 1710, 2396, 2598
Tru9I TTAA 8 cut(s) 419, 926, 1341, 1355, 1406, 1710, 2396, 2598
TscAI CASTG 2 cut(s) 1927, 2574
TseFI GTSAC 3 cut(s) 768, 1148, 1177
TseI GCWGC 5 cut(s) 39, 112, 581, 1365, 1917
Tsp45I GTSAC 3 cut(s) 768, 1148, 1177
TspGWI ACGGA 1 cut(s) 2242
TspMI CCCGGG 1 cut(s) 2550
TspRI CASTG 2 cut(s) 1927, 2574
VpaK11BI GGWCC 6 cut(s) 173, 260, 935, 1508, 1563, 2387
VspI ATTAAT 1 cut(s) 2598
XagI CCTNNNNNAGG 1 cut(s) 659
XapI RAATTY 8 cut(s) 421, 563, 874, 1198, 1239, 1268, 1779, 2408
XceI RCATGY 1 cut(s) 1673
XcmI CCANNNNNNNNNTGG 1 cut(s) 1628
XhoI CTCGAG 1 cut(s) 2069
XmaI CCCGGG 1 cut(s) 2550
XmiI GTMKAC 1 cut(s) 2518
XmnI GAANNNNTTC 2 cut(s) 1014, 1318
XspI CTAG 6 cut(s) 1182, 1287, 1385, 1548, 1899, 2135
Zsp2I ATGCAT 1 cut(s) 2095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.