MD15G1231000.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005501
Physical Location & Seq
Reverse (-)
18906109 .. 18911108
5000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1231000.v1.1.491

Sequence Viewer

Length: 2595 bp
ATGGGAGGCTTATCGCACCTTTTTGATTTCAACCAGGGCAGCATGGCCAGAAAGCTTTTTACACATAAGAAGCATGATGGCGGCCTTGATGCTCCAAGAAATAGCCTGGAGATGCAGGTAGAGCCTCAGAGCTATTGTGACACAGGAGATTTGCCGGTTGAAGAAAACTGGTCTAGAAAGAATAATCCATCGGAAAGTTCAATGAAGAAATTGATTAATGAGGAAATATCCAAACATTCGAGTACCAGACAAAATGCACCAAACATTGTGGCCCGACTGATGGGGATGGATATGTTGCCAGTGGAACCAAAATCTGTAGTTGTGGAAGAAAAAGGTGAAAATAGGAGAACGAAATCTTCAAAGAAGGAAATGAATGGAAGGAGCTCAGTCGGTCGCGTCTCTTCTAACTTAAATTCCTCCAAGCAGATGGATATTGATTCTTATTATCACAGTAATGGCAGAGATGCCACTAGGTGGAGCGATGACCAGAAAATCGAAAATCCAAGGCGTAGAGAACATCCTCAAGAGGAGGAACTACAAAAGTTCAAGAAAGAATTTGAAGCATGGCAAGCTTCCAGGTTTAGGGAATGCTCAAGGTTTCTCAAAATTGATAGCAACCTCAACGAGGAAAAGATGGCAGTTTCAGGGCATAGAACAACTCAGACAACTTTAGAACGTAAGGACCACGCAGTAAAAGAAATCTCACATGAAGAAAGTGATTTACAACGTCGAGGAAATGAGTTTTCGGAATTATTCCCAGCTGAGCAAAAGGGTTCTTTTTCTTCAAGAAGCAGAAGAACCATGAGCTTAGACTTTGAGCAATCTTCCCTGGTGAGTTCTAAGAAGAGATTTGAGTCATTCGACGCGTCTTCTCCTCCTACAAGGATAGTTATCTTAAGACCTGGTCCCGATAGACTTTGCAACCATGAAGAGACTTGGACAGGTTCTTCAAACACTCTAGAGAGGAAGGGTGGCATAGAAGATTTTCTCGAGGAGGTGAAAGAACGACTAAGATCTGAGCTGCAAGGGAAAATGCATAAAAGGGGTTCTGTTGTCCGAGGAAGTGGAATTGAGACCCCGTTTAGCGAACAGCCATCATCTCGAAAGCAGATTGCTCGACACATTGCAAACCAGGTCAGGGAAAGTGCGACGAGGGACCTTGGCATGAATTTGCTGAGATCAGAATCGACAAGATCATACAGAAGCGAAATGCAGTTCAATGGATCAGGTTCCCCGGAATTTATGCATAGAGATACTAAAAGAATTTTGTTAGAGAGACCGAGAAATGTTTCAAAAATAGAAACAGAACCTCGTGATCCTTCATTAGTTTCTGGGAGATCTAGGTTATCTGCTTTAGACTACGAAAGGGAAAGGGGAGTAGTGAAAGATGAACATGAAAACACAAGATCATTCAGATATGGATCACATGGAGATGAAGTGCTTGATAGAGAGTTGTCCCCTAGAAACCTCATTAGGTCCTTATCCGCCCCAGTACCCGGAACATCTTTTGGGAAACTTCTTCTGGAGGACCGTCATGTTTTAACCGGTGCTCACATTCAGAGGAAGCATGAAGGCATTGGACCTGTGTCCATGGAGATGAAACATCAGAAGAAAGAAAGGTTTAATTTTAAAGAAAAAGTTTCCAATCTCCGGTACAGTTTTATGCTTAGGGGGAGACTTTTTGGCAAAAAAAACCAATCAGTTCCGGAATCACGTCATACTGAACGGTATCCTATGAAGGATATCATGAGCGGGCCAACAGTGATTACAAACTCTGGAGAGAGACATGATAATTTCACCGAGGTGCCTCCTAGTCCTGCATCTGCATGCAGCACTGCTCGGGAAGAGTTCTGGAGGCCAACTGATTATCTTAGTCCAATATCAACTCCGAGTGCAACTCCACGAGAAGATAGTATCGTGCCACAGGCTTTTAGGGACATCAGCGATAATCTGAGTGAGCTACGAAGGCAACTGAACCAACTCGAGTCTAATGAGCCTGAGGGGATTAAAGTTGAGGAAGCGGTTGAGTCTGAAATGGCTAGTTTAGACAACCCAGCAGAGGTTTACATACGAGATTTGCTTGTCGTTTCTGGTTTGTACGATGGATCATTTGATAAATCACTATCGAGATGGGACACATTTGCGAAACCAATCAGCAACTCAGTGTTCGAAGAAGTGGAAGAATCGTATAAAAACTTGGCCAACAAGCAAGATCCTTCAGCAACAGATCACAAGGAGATGGTAAATCACAAGGTGTTACGCGACTTGTTGAACGAATCTCTTTCAACAGTTCTCGGACCAGCAATAAGCATGCCAAACTTCCGGAGAAAAATGATCAACTCCTCTGCGCTGCCACCATTACGAGGCAAGAAGTTGTTGAATTGTGTGTGGCGAATCATCTGCGAATGCCTATATCCTCCAACTAATGTACCCTATTACTCACTCGATGATATGGCCGCCCGGGATCTTGGATCAGTTCCTTGGTCCGGACTGATGGACGAGGACGTTAATGCAGTGGGACAAGAGATCGAAAGCCTGATCATGGGAGATCTTGTTCAGGAAATTTTGTATGATATGCAGTTATTAATGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 NTPase_1 VARLMGL

Protein Analysis

865

Amino Acids

98.61

Weight (kDa)

6.69

Isoelectric Point (pI)

64.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 688 - 854 5.4e-30 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 1804
AccB7I CCANNNNNTGG 1 cut(s) 474
AccBSI CCGCTC 1 cut(s) 1752
AccII CGCG 3 cut(s) 396, 866, 2262
AccIII TCCGGA 3 cut(s) 1705, 2322, 2486
AciI CCGC 5 cut(s) 81, 1485, 1752, 2021, 2457
AclWI GGATC 7 cut(s) 1231, 1310, 1429, 2113, 2207, 2472, 2479
AcoI YGGCCR 3 cut(s) 45, 2199, 2454
AcsI RAATTY 6 cut(s) 412, 554, 1168, 1238, 1263, 2562
AcuI CTGAAG 1 cut(s) 2202
AdeI CACNNNGTG 2 cut(s) 474, 2254
AfaI GTAC 5 cut(s) 244, 1494, 1655, 2099, 2430
AflII CTTAAG 1 cut(s) 895
AflIII ACRYGT 1 cut(s) 864
AgeI ACCGGT 1 cut(s) 1544
AjiI CACGTC 1 cut(s) 1715
AjnI CCWGG 6 cut(s) 33, 105, 575, 828, 901, 1131
AleI CACNNNNGTG 1 cut(s) 1802
AloI GAACNNNNNNTCC 2 cut(s) 2538, 2570
AluBI AGCT 8 cut(s) 55, 132, 384, 572, 761, 807, 1021, 1960
AluI AGCT 8 cut(s) 55, 132, 384, 572, 761, 807, 1021, 1960
Alw21I GWGCWC 2 cut(s) 386, 1552
Alw26I GTCTC 6 cut(s) 403, 926, 1067, 1270, 1669, 1777
AlwI GGATC 7 cut(s) 1231, 1310, 1429, 2113, 2207, 2472, 2479
Ama87I CYCGRG 4 cut(s) 989, 1839, 1982, 2460
Aor13HI TCCGGA 3 cut(s) 1705, 2322, 2486
AoxI GGCC 7 cut(s) 45, 82, 270, 1754, 1856, 2199, 2454
ApeKI GCWGC 4 cut(s) 39, 1021, 1830, 2350
ApoI RAATTY 6 cut(s) 412, 554, 1168, 1238, 1263, 2562
AseI ATTAAT 2 cut(s) 216, 2585
AsiGI ACCGGT 1 cut(s) 1544
Asp700I GAANNNNTTC 2 cut(s) 984, 1288
AspLEI GCGC 1 cut(s) 2350
AsuC2I CCSGG 4 cut(s) 1235, 1497, 2461, 2462
AsuHPI GGTGA 4 cut(s) 347, 844, 1009, 1789
AsuII TTCGAA 1 cut(s) 2169
AvaI CYCGRG 4 cut(s) 989, 1839, 1982, 2460
AvaII GGWCC 8 cut(s) 682, 905, 1156, 1476, 1528, 1580, 2297, 2484
AxyI CCTNAGG 1 cut(s) 1998
BaeI ACNNNNGTAYC 2 cut(s) 2412, 2445
BalI TGGCCA 2 cut(s) 47, 2201
BanI GGYRCC 1 cut(s) 1804
BanII GRGCYC 1 cut(s) 386
BauI CACGAG 2 cut(s) 1311, 1902
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 386, 1552
BbvI GCAGC 4 cut(s) 51, 1008, 1842, 2337
BcgI CGANNNNNNTGC 4 cut(s) 1097, 1131, 2382, 2416
BciT130I CCWGG 6 cut(s) 35, 107, 577, 830, 903, 1133
BciVI GTATCC 1 cut(s) 1740
BclI TGATCA 2 cut(s) 2334, 2538
BcnI CCSGG 4 cut(s) 1235, 1497, 2461, 2462
BcoDI GTCTC 6 cut(s) 403, 926, 1067, 1270, 1669, 1777
BfaI CTAG 7 cut(s) 174, 471, 959, 1341, 1461, 1812, 2040
BfmI CTRYAG 1 cut(s) 315
BfrI CTTAAG 1 cut(s) 895
BfuAI ACCTGC 1 cut(s) 106
BfuI GTATCC 1 cut(s) 1740
BglII AGATCT 3 cut(s) 1013, 1337, 2548
BisI GCNGC 6 cut(s) 40, 82, 1022, 1831, 2351, 2457
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 6 cut(s) 41, 83, 1023, 1832, 2352, 2458
Bme18I GGWCC 8 cut(s) 682, 905, 1156, 1476, 1528, 1580, 2297, 2484
BmeT110I CYCGRG 4 cut(s) 989, 1839, 1982, 2460
BmgBI CACGTC 1 cut(s) 1715
BmiI GGNNCC 5 cut(s) 306, 907, 1157, 1231, 1806
BmrI ACTGGG 1 cut(s) 1484
BmsI GCATC 4 cut(s) 79, 102, 454, 1829
BmuI ACTGGG 1 cut(s) 1484
BoxI GACNNNNGTC 1 cut(s) 1585
BpiI GAAGAC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 1882, 1914
BpmI CTGGAG 4 cut(s) 128, 1544, 1797, 1873
Bpu10I CCTNAGC 1 cut(s) 1667
Bpu1102I GCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 2169
BpuEI CTTGAG 2 cut(s) 507, 577
BpuMI CCSGG 4 cut(s) 1235, 1497, 2461, 2462
BsaBI GATNNNNATC 3 cut(s) 890, 1183, 1420
BsaI GGTCTC 2 cut(s) 1067, 1270
BsaWI WCCGGW 5 cut(s) 1544, 1650, 1705, 2322, 2486
BsaXI ACNNNNNCTCC 4 cut(s) 1422, 1452, 2538, 2568
Bse118I RCCGGY 2 cut(s) 154, 1544
Bse1I ACTGG 3 cut(s) 173, 299, 1490
Bse21I CCTNAGG 1 cut(s) 1998
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 3 cut(s) 890, 1183, 1420
BseAI TCCGGA 3 cut(s) 1705, 2322, 2486
BseBI CCWGG 6 cut(s) 35, 107, 577, 830, 903, 1133
BseGI GGATG 2 cut(s) 291, 517
BseJI GATNNNNATC 3 cut(s) 890, 1183, 1420
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 9 cut(s) 140, 399, 674, 753, 1008, 1166, 1943, 1989, 2175
BseNI ACTGG 3 cut(s) 173, 299, 1490
BseRI GAGGAG 4 cut(s) 542, 864, 1007, 2332
BseXI GCAGC 4 cut(s) 51, 1008, 1842, 2337
BseYI CCCAGC 2 cut(s) 757, 2053
Bsh1236I CGCG 3 cut(s) 396, 866, 2262
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 7 cut(s) 47, 84, 272, 1756, 1858, 2201, 2456
BshNI GGYRCC 1 cut(s) 1804
BshTI ACCGGT 1 cut(s) 1544
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 386, 1552
BsiHKCI CYCGRG 4 cut(s) 989, 1839, 1982, 2460
BsiSI CCGG 9 cut(s) 155, 1235, 1497, 1545, 1651, 1706, 2323, 2461, 2487
BslFI GGGAC 6 cut(s) 891, 1169, 1441, 1949, 2147, 2532
BsmAI GTCTC 6 cut(s) 403, 926, 1067, 1270, 1669, 1777
BsmBI CGTCTC 1 cut(s) 403
BsmFI GGGAC 6 cut(s) 891, 1169, 1441, 1949, 2147, 2532
BsmI GAATGC 2 cut(s) 593, 2411
BsnI GGCC 7 cut(s) 47, 84, 272, 1756, 1858, 2201, 2456
Bso31I GGTCTC 2 cut(s) 1067, 1270
BsoBI CYCGRG 4 cut(s) 989, 1839, 1982, 2460
Bsp119I TTCGAA 1 cut(s) 2169
Bsp1286I GDGCHC 2 cut(s) 386, 1552
Bsp13I TCCGGA 3 cut(s) 1705, 2322, 2486
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 1590
BspACI CCGC 5 cut(s) 81, 1485, 1752, 2021, 2457
BspANI GGCC 7 cut(s) 47, 84, 272, 1756, 1858, 2201, 2456
BspCNI CTCAG 9 cut(s) 139, 398, 673, 754, 1009, 1167, 1944, 1990, 2174
BspEI TCCGGA 3 cut(s) 1705, 2322, 2486
BspFNI CGCG 3 cut(s) 396, 866, 2262
BspHI TCATGA 1 cut(s) 1746
BspLI GGNNCC 5 cut(s) 306, 907, 1157, 1231, 1806
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 7 cut(s) 1231, 1310, 1429, 2113, 2207, 2472, 2479
BspT104I TTCGAA 1 cut(s) 2169
BspT107I GGYRCC 1 cut(s) 1804
BspTI CTTAAG 1 cut(s) 895
BspTNI GGTCTC 2 cut(s) 1067, 1270
BsrBI CCGCTC 1 cut(s) 1752
BsrDI GCAATG 1 cut(s) 1122
BsrFI RCCGGY 2 cut(s) 154, 1544
BsrI ACTGG 3 cut(s) 173, 299, 1490
BssAI RCCGGY 2 cut(s) 154, 1544
BssSI CACGAG 2 cut(s) 1311, 1902
BssT1I CCWWGG 4 cut(s) 503, 1159, 1590, 2480
Bst2BI CACGAG 2 cut(s) 1311, 1902
Bst2UI CCWGG 6 cut(s) 35, 107, 577, 830, 903, 1133
Bst4CI ACNGT 6 cut(s) 452, 1532, 1658, 1728, 1762, 2290
Bst6I CTCTTC 4 cut(s) 406, 839, 924, 1839
BstAFI CTTAAG 1 cut(s) 895
BstBI TTCGAA 1 cut(s) 2169
BstC8I GCNNGC 4 cut(s) 570, 1754, 1828, 2312
BstDSI CCRYGG 1 cut(s) 1590
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 2 cut(s) 291, 517
BstFNI CGCG 3 cut(s) 396, 866, 2262
BstHHI GCGC 1 cut(s) 2350
BstMAI GTCTC 6 cut(s) 403, 926, 1067, 1270, 1669, 1777
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 3 cut(s) 121, 569, 1966
BstNI CCWGG 6 cut(s) 35, 107, 577, 830, 903, 1133
BstNSI RCATGY 2 cut(s) 1830, 2314
BstPAI GACNNNNGTC 1 cut(s) 1585
BstSFI CTRYAG 1 cut(s) 315
BstUI CGCG 3 cut(s) 396, 866, 2262
BstV1I GCAGC 4 cut(s) 51, 1008, 1842, 2337
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 5 cut(s) 1013, 1337, 2212, 2464, 2548
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 5 cut(s) 1013, 1337, 2212, 2464, 2548
Bsu36I CCTNAGG 1 cut(s) 1998
BsuI GTATCC 1 cut(s) 1740
BsuRI GGCC 7 cut(s) 47, 84, 272, 1756, 1858, 2201, 2456
BtgI CCRYGG 1 cut(s) 1590
BtgZI GCGATG 1 cut(s) 495
BtrI CACGTC 1 cut(s) 1715
BtsCI GGATG 2 cut(s) 291, 517
BtsI GCAGTG 2 cut(s) 1833, 2520
BtsIMutI CAGTG 5 cut(s) 306, 1767, 1833, 2169, 2520
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 4 cut(s) 570, 1754, 1828, 2312
CciI TCATGA 1 cut(s) 1746
CfoI GCGC 1 cut(s) 2350
Cfr10I RCCGGY 2 cut(s) 154, 1544
Cfr9I CCCGGG 1 cut(s) 2460
CseI GACGC 3 cut(s) 385, 855, 872
CsiI ACCWGGT 2 cut(s) 901, 1131
Csp6I GTAC 5 cut(s) 243, 1493, 1654, 2098, 2429
CspAI ACCGGT 1 cut(s) 1544
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviQI GTAC 5 cut(s) 243, 1493, 1654, 2098, 2429
DraI TTTAAA 1 cut(s) 1630
DraIII CACNNNGTG 2 cut(s) 474, 2254
EaeI YGGCCR 3 cut(s) 45, 2199, 2454
Eam1104I CTCTTC 4 cut(s) 406, 839, 924, 1839
EarI CTCTTC 4 cut(s) 406, 839, 924, 1839
EciI GGCGGA 1 cut(s) 1474
Ecl136II GAGCTC 1 cut(s) 384
Eco130I CCWWGG 4 cut(s) 503, 1159, 1590, 2480
Eco24I GRGCYC 1 cut(s) 386
Eco31I GGTCTC 2 cut(s) 1067, 1270
Eco32I GATATC 1 cut(s) 1744
Eco47I GGWCC 8 cut(s) 682, 905, 1156, 1476, 1528, 1580, 2297, 2484
Eco53kI GAGCTC 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 2202
Eco81I CCTNAGG 1 cut(s) 1998
Eco88I CYCGRG 4 cut(s) 989, 1839, 1982, 2460
EcoICRI GAGCTC 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 2 cut(s) 1156, 1476
EcoRII CCWGG 6 cut(s) 33, 105, 575, 828, 901, 1131
EcoRV GATATC 1 cut(s) 1744
EcoT14I CCWWGG 4 cut(s) 503, 1159, 1590, 2480
EcoT22I ATGCAT 2 cut(s) 1038, 1248
EcoT38I GRGCYC 1 cut(s) 386
ErhI CCWWGG 4 cut(s) 503, 1159, 1590, 2480
Esp3I CGTCTC 1 cut(s) 403
FaqI GGGAC 6 cut(s) 891, 1169, 1441, 1949, 2147, 2532
FauI CCCGC 1 cut(s) 1745
FbaI TGATCA 2 cut(s) 2334, 2538
Fnu4HI GCNGC 6 cut(s) 40, 82, 1022, 1831, 2351, 2457
FokI GGATG 2 cut(s) 298, 504
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 6 cut(s) 40, 82, 1022, 1831, 2351, 2457
FspBI CTAG 7 cut(s) 174, 471, 959, 1341, 1461, 1812, 2040
GlaI GCGC 1 cut(s) 2349
GluI GCNGC 6 cut(s) 40, 82, 1022, 1831, 2351, 2457
GsaI CCCAGC 2 cut(s) 761, 2057
GsuI CTGGAG 4 cut(s) 128, 1544, 1797, 1873
HaeIII GGCC 7 cut(s) 47, 84, 272, 1756, 1858, 2201, 2456
HapII CCGG 9 cut(s) 155, 1235, 1497, 1545, 1651, 1706, 2323, 2461, 2487
HgaI GACGC 3 cut(s) 385, 855, 872
HhaI GCGC 1 cut(s) 2350
Hin6I GCGC 1 cut(s) 2348
HinP1I GCGC 1 cut(s) 2348
HindIII AAGCTT 2 cut(s) 53, 570
HinfI GANTC 9 cut(s) 437, 854, 1184, 1709, 1985, 2027, 2183, 2276, 2394
HpaII CCGG 9 cut(s) 155, 1235, 1497, 1545, 1651, 1706, 2323, 2461, 2487
HphI GGTGA 4 cut(s) 347, 844, 1009, 1789
Hpy166II GTNNAC 1 cut(s) 2065
Hpy8I GTNNAC 1 cut(s) 2065
Hpy99I CGWCG 3 cut(s) 732, 866, 1153
HpyAV CCTTC 8 cut(s) 358, 372, 961, 1329, 1565, 1732, 1959, 2226
HpyCH4III ACNGT 6 cut(s) 452, 1532, 1658, 1728, 1762, 2290
HpyCH4IV ACGT 4 cut(s) 676, 727, 1714, 2505
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 569, 1966
HpySE526I ACGT 4 cut(s) 676, 727, 1714, 2505
HspAI GCGC 1 cut(s) 2348
Kpn2I TCCGGA 3 cut(s) 1705, 2322, 2486
Ksp22I TGATCA 2 cut(s) 2334, 2538
LmnI GCTCC 3 cut(s) 97, 381, 477
Lsp1109I GCAGC 4 cut(s) 51, 1008, 1842, 2337
LweI GCATC 4 cut(s) 79, 102, 454, 1829
MabI ACCWGGT 2 cut(s) 901, 1131
MaeI CTAG 7 cut(s) 174, 471, 959, 1341, 1461, 1812, 2040
MaeII ACGT 4 cut(s) 676, 727, 1714, 2505
MaeIII GTNAC 2 cut(s) 137, 2256
MbiI CCGCTC 1 cut(s) 1752
MflI RGATCY 5 cut(s) 1013, 1337, 2212, 2464, 2548
MhlI GDGCHC 2 cut(s) 386, 1552
MlsI TGGCCA 2 cut(s) 47, 2201
MluI ACGCGT 1 cut(s) 864
MluNI TGGCCA 2 cut(s) 47, 2201
MlyI GAGTC 3 cut(s) 863, 1994, 2036
MmeI TCCRAC 1 cut(s) 2444
Mox20I TGGCCA 2 cut(s) 47, 2201
Mph1103I ATGCAT 2 cut(s) 1038, 1248
MroI TCCGGA 3 cut(s) 1705, 2322, 2486
MroXI GAANNNNTTC 2 cut(s) 984, 1288
MscI TGGCCA 2 cut(s) 47, 2201
MseI TTAA 9 cut(s) 216, 410, 896, 1541, 1623, 1629, 2007, 2508, 2585
MslI CAYNNNNRTG 5 cut(s) 453, 1431, 1595, 1802, 1825
Msp20I TGGCCA 2 cut(s) 47, 2201
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 1 cut(s) 895
MspI CCGG 9 cut(s) 155, 1235, 1497, 1545, 1651, 1706, 2323, 2461, 2487
Mva1269I GAATGC 2 cut(s) 593, 2411
MvaI CCWGG 6 cut(s) 35, 107, 577, 830, 903, 1133
MvnI CGCG 3 cut(s) 396, 866, 2262
MwoI GCNNNNNNNGC 3 cut(s) 121, 569, 1966
NciI CCSGG 4 cut(s) 1235, 1497, 2461, 2462
NcoI CCATGG 1 cut(s) 1590
NlaIV GGNNCC 5 cut(s) 306, 907, 1157, 1231, 1806
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 2 cut(s) 1038, 1248
NspI RCATGY 2 cut(s) 1830, 2314
NspV TTCGAA 1 cut(s) 2169
OliI CACNNNNGTG 1 cut(s) 1802
PaeI GCATGC 2 cut(s) 1830, 2314
PaeR7I CTCGAG 2 cut(s) 989, 1982
PagI TCATGA 1 cut(s) 1746
PctI GAATGC 2 cut(s) 593, 2411
PdmI GAANNNNTTC 2 cut(s) 984, 1288
PfeI GAWTC 6 cut(s) 437, 1184, 1709, 2183, 2276, 2394
PflFI GACNNNGTC 1 cut(s) 903
PflMI CCANNNNNTGG 1 cut(s) 474
PinAI ACCGGT 1 cut(s) 1544
PkrI GCNGC 6 cut(s) 41, 83, 1023, 1832, 2352, 2458
PleI GAGTC 3 cut(s) 862, 1993, 2035
PpsI GAGTC 3 cut(s) 862, 1993, 2035
PpuMI RGGWCCY 2 cut(s) 1156, 1476
PshAI GACNNNNGTC 1 cut(s) 1585
PshBI ATTAAT 2 cut(s) 216, 2585
Psp124BI GAGCTC 1 cut(s) 386
Psp5II RGGWCCY 2 cut(s) 1156, 1476
Psp6I CCWGG 6 cut(s) 33, 105, 575, 828, 901, 1131
PspFI CCCAGC 2 cut(s) 757, 2053
PspGI CCWGG 6 cut(s) 33, 105, 575, 828, 901, 1131
PspN4I GGNNCC 5 cut(s) 306, 907, 1157, 1231, 1806
PspPPI RGGWCCY 2 cut(s) 1156, 1476
PspXI VCTCGAGB 1 cut(s) 1982
PsuI RGATCY 5 cut(s) 1013, 1337, 2212, 2464, 2548
PsyI GACNNNGTC 1 cut(s) 903
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 5 cut(s) 244, 1494, 1655, 2099, 2430
RsaNI GTAC 5 cut(s) 243, 1493, 1654, 2098, 2429
RseI CAYNNNNRTG 5 cut(s) 453, 1431, 1595, 1802, 1825
SacI GAGCTC 1 cut(s) 386
SaqAI TTAA 9 cut(s) 216, 410, 896, 1541, 1623, 1629, 2007, 2508, 2585
SatI GCNGC 6 cut(s) 40, 82, 1022, 1831, 2351, 2457
SchI GAGTC 3 cut(s) 863, 1994, 2036
SduI GDGCHC 2 cut(s) 386, 1552
SexAI ACCWGGT 2 cut(s) 901, 1131
SfaNI GCATC 4 cut(s) 79, 102, 454, 1829
SfcI CTRYAG 1 cut(s) 315
Sfr274I CTCGAG 2 cut(s) 989, 1982
SfuI TTCGAA 1 cut(s) 2169
SinI GGWCC 8 cut(s) 682, 905, 1156, 1476, 1528, 1580, 2297, 2484
SlaI CTCGAG 2 cut(s) 989, 1982
SmaI CCCGGG 1 cut(s) 2462
SmiMI CAYNNNNRTG 5 cut(s) 453, 1431, 1595, 1802, 1825
SmlI CTYRAG 5 cut(s) 522, 592, 895, 989, 1982
SmoI CTYRAG 5 cut(s) 522, 592, 895, 989, 1982
SphI GCATGC 2 cut(s) 1830, 2314
SsiI CCGC 5 cut(s) 81, 1485, 1752, 2021, 2457
SspMI CTAG 7 cut(s) 174, 471, 959, 1341, 1461, 1812, 2040
SstI GAGCTC 1 cut(s) 386
StyI CCWWGG 4 cut(s) 503, 1159, 1590, 2480
TaaI ACNGT 6 cut(s) 452, 1532, 1658, 1728, 1762, 2290
TaiI ACGT 4 cut(s) 679, 730, 1717, 2508
TaqII GACCGA 2 cut(s) 380, 1294
TauI GCSGC 2 cut(s) 84, 2459
TfiI GAWTC 6 cut(s) 437, 1184, 1709, 2183, 2276, 2394
Tru1I TTAA 9 cut(s) 216, 410, 896, 1541, 1623, 1629, 2007, 2508, 2585
Tru9I TTAA 9 cut(s) 216, 410, 896, 1541, 1623, 1629, 2007, 2508, 2585
TscAI CASTG 5 cut(s) 306, 1767, 1840, 2169, 2520
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 4 cut(s) 39, 1021, 1830, 2350
Tsp45I GTSAC 1 cut(s) 137
TspMI CCCGGG 1 cut(s) 2460
TspRI CASTG 5 cut(s) 306, 1767, 1840, 2169, 2520
Tth111I GACNNNGTC 1 cut(s) 903
Van91I CCANNNNNTGG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 895
VpaK11BI GGWCC 8 cut(s) 682, 905, 1156, 1476, 1528, 1580, 2297, 2484
VspI ATTAAT 2 cut(s) 216, 2585
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 6 cut(s) 412, 554, 1168, 1238, 1263, 2562
XbaI TCTAGA 2 cut(s) 173, 958
XceI RCATGY 2 cut(s) 1830, 2314
XhoI CTCGAG 2 cut(s) 989, 1982
XmaI CCCGGG 1 cut(s) 2460
XmnI GAANNNNTTC 2 cut(s) 984, 1288
XspI CTAG 7 cut(s) 174, 471, 959, 1341, 1461, 1812, 2040
Zsp2I ATGCAT 2 cut(s) 1038, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.