Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004440

Physical Location & Seq

Genomic Coordinates Reverse (-)

13475115 .. 13478862

Gene Structure 3748 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1160700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGCATTTTTCACTGTGGAAGTCCTTCTCCCACTGCGTTGCTCTGTTCCTGGACAAGAAGAGCAGCAGCAGAAGAAGGAAAGATGGGTCGGATTCGAACTCCGACGCCAAGAAAAACTCGTCAATTCTCCGGAAGCTTCAAGAAAACAAGCTCAGGGAGGCTCTGGAGCAAGCTTCGGAAGATGGGTTTCTCTTCAAATCTCAGGACATGGAGTCTGAGGCCATTGCCTACCGGGACGAGGGGATTGGACGGTCCAGATCGCTCGCTAGGCTCCATGCCCAGCGGGAGTTTCTTTGTGCCACTGCTCTTGCTGCTGACCGGATTTTCGAGTCCGAAAACGCCATCCCTGAGCTCCATGAAGCGTTCACAAAGTTCCTCACTATGTACCCGGATTACCAGTCTTCGGAGAAGATCGATCAGCTGAGGGTGGAGGAGTATGCACATTTGTCTCCAAAGGTATGCCTTGATTACTGCGGTTTTGGGCTTTTTTCGTACCTTCAGACACTGCACTATTGGGATTCTTCTACATTTAGCTTGTCTGAGATAACTGCAAATTTGAATAATCATGCACTTTATGGTGGTGCTGAGAAAGGCACTGTGGAACATGATATAAAAACTAGGATTGTGGATTATTTGAACATTCCTGAGAGTGAATATGGTCTTGTTTTTACTTCTGTGAATTGGATGGCTCAGAGTGCTAGAGAGAAAGGTGCAAAGGTGCACAGTGCTTGGTTTAAATGGCCAACCCTCAAACTCTGTTCCGCTGATTTGAGAAAGGAGATTTCGATTAAGAAGAGGAGGAAGAAGGATTCCGCGGCTGGTCTTTTTGTGTTTCCGGTGCAGTCTAGAGTTACTGGGGCCAAGTATTCTTACCAATGGATGGCACTGGCTCAGCAGAACAATTGGCATGTCTTACTTGATGCCGGGTCTTTGGGTCCCAAAGACATGGATTCACTTGGACTGTCGCTTTTCCGGCCTGATTTTATCATCACATCGTTTTACAAGGTATTTGGGTTTGATCCAACTGGATTTGGCTGCCTTCTGATTAAGAAATCAGTAATCGGATGCCTTCAAAATCAGTCAGGGTCAACTGGGTCTGGAATGGTGAAGATTACCCCTGAATACCCTTTATATCTTAGTGATTCGGTTGATGGTATGGACAAATTGGCTGGAACTGAGGATGATGAAGTAACTGAAAATGGTGAGATAACCACTGAAACTCGGCAGGGAAAGCAGATGCCTGCATTCTCTGGAGCATACACATCTTCTCAGGTGAGGGATGTATTTGAGACTGAGATGGATCACGATAACAGCTCAGACAGGGATGGGACGAGCACCTTATTTGAAGAAGCTGAGAGTGTCTCAGCGGGGGAGCTTATGAAGAGTCCAATCTTCAGTGAAGACTATTCATCAGACAATTCGTATTGGATTGGTTTGGGTCAGAGCCCAATGGGTTCCGACCATTCTGGTCATATTAACAAACATAAGATTGCCTCTCCTCAGCCTCCATTTTGGTTTTCTGGCAGGAAGAATCGTAAGCAAGTTTCCCCAAAACCTGCTTCTAGGCTGTATGGTAGCCCCATATTTGATGATAAAAAGGCAAACTCAGGGCTACATGATGACTCTCATGTGCTATCATTTGACGCCGCCGTCCTATCAATTTCTCAGGAGCTGGACTGTGTCAAGGAGGTCAATGAAGAACAATTTGCAGAAGCATGTCCCACTTCACGAAATGGTGAAAGGGCTTCTGATCATCATAATGGAGAGATCCAAGAAGAATGTGGAACTATCGAACCCTTGTCCAATGGGTCAGTGTTTAATTCTGGCACAAATGGATTTTGTCATGAAAATTCAACTTCCACTTATCAGCATCAGAACCTAGAAAATGGATCAACCATTGGAAGTTGCACAGAGATTAGTGGTGGTGCCATAAGAAGAGAAACTGAAGGTGAATTTAGGTTGTTAGGGAGAAGGGAGGGGAGTAAATATGGAAATGGTAGATTTTTTAGTTTGGAAGAGAATGAGCAACCAAGCCGGGGGAGAAGGGTGTCTTTTAGTGCGGAAAAAGACCACGTAAGCCATAATCTTGAGACAGGAGAAATTTCTGCAACTAGTCTGGAGGATGAAGAGTCCGCTAGTGATGGAGCATATGGTGATGGGCAAGACTTGGACAGGAGGGAACCTGAGATAATATGCCGGCATCTTGATCATGTTAATACGTTAGGTCTCAATAAAACCACCTCCCGGCTGCGATTTTTGGCCAATTGGCTTGTGACCTCCTTGCTACAACTAAGATTGCGTGGATCAGATGAAGACAAAAGAACAAATCTTGTTGACATCTATGGCCCCAAAATAAAATACGAAAGGGGAGCAGCTGTAGCTTTTAATGTGAAAGACCAGAATAGAGGGCTAATCAATCCAGAAGTTGTCCAGAAGCTGGCTGAAAAAGAAGGAATTGCACTTGGTGTTGGTATCCTCAGTCACATACGAATCATGGATAGTCCAAGACAGAAAAATGGTGTTTTGAATCTTCAAGATGCTACCCTTTGCAGGCCAATGGAGAACGGCAGAAATGGTAAAAAAAGCGAGTTTGTACGCCTTGAGGTTGTGACGGCTTCTCTGGGATTCCTTACCAACTTTGAAGATGTTTACAAGTTATGGGCTTTTATAGCAAATTTTCTTAATCCAACCTTTATCAGAGAGGGTGGGCTGTCAACTGTCTTTGAAGAATCTGAGACGAAATCTGTAACATCTGTCCATGATATTGATCAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

No domains found.

Protein Analysis

926

Amino Acids

103.27

Weight (kDa)

5.52

Isoelectric Point (pI)

46.84

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004440)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23520
fragaria_vesca	FvH4_1g14760
malus_domestica	MD02G1160700.v1.1 MD02G1160800.v1.1 MD15G1274900.v1.1
prunus_persica	Prupe.7G142800_v2.0.a1 Prupe.7G142900_v2.0.a1
pyrus_communis	pycom02g12670 pycom02g12680 pycom02g12690 pycom15g23800
rosa_chinensis	RchiOBHm_Chr2g0103691 RchiOBHm_Chr2g0103701
rosa_laevigata	RLG00000017249 RLG00000017250
rosa_multiflora	Rmu_sc0001852.1_g000009 Rmu_sc0001852.1_g000010
rosa_roxburghii	Rroxscaffold_2G00139470 Rroxscaffold_2G00139480 Rroxscaffold_5G00365360
rosa_rugosa	Rorug02G0116600 Rorug02G0116700
rosa_samantha	Rh2AG165300 Rh2AG165400 Rh2BG172500 Rh2BG172600 Rh2CG171400 Rh2CG171500 Rh2DG170900 Rh2DG171000
rosa_wichuraiana	Rw2G012950 Rw2G012960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1649
Acc36I	ACCTGC	1 cut(s)	1564
AccB1I	GGYRCC	1 cut(s)	1925
AccB7I	CCANNNNNTGG	2 cut(s)	880, 2437
AccII	CGCG	1 cut(s)	815
AccIII	TCCGGA	1 cut(s)	129
AciI	CCGC	9 cut(s)	283, 474, 762, 813, 815, 1367, 1647, 2060, 2133
AclWI	GGATC	5 cut(s)	1013, 1308, 1762, 1897, 2311
AcoI	YGGCCR	2 cut(s)	740, 2259
AcsI	RAATTY	5 cut(s)	553, 1849, 1952, 2100, 2674
AcuI	CTGAAG	3 cut(s)	482, 1378, 1965
AcyI	GRCGYC	2 cut(s)	105, 1644
AdeI	CACNNNGTG	1 cut(s)	2465
AfaI	GTAC	3 cut(s)	386, 494, 2595
AfiI	CCNNNNNNNGG	7 cut(s)	238, 403, 880, 2036, 2244, 2437, 2550
AhdI	GACNNNNNGTC	1 cut(s)	211
AhlI	ACTAGT	1 cut(s)	2111
AjnI	CCWGG	1 cut(s)	48
AjuI	GAANNNNNNNTTGG	2 cut(s)	1543, 1575
Alw21I	GWGCWC	3 cut(s)	354, 723, 1337
Alw26I	GTCTC	6 cut(s)	453, 1283, 1366, 2084, 2231, 2729
Alw44I	GTGCAC	1 cut(s)	719
AlwI	GGATC	5 cut(s)	1013, 1308, 1762, 1897, 2311
AlwNI	CAGNNNCTG	3 cut(s)	505, 1672, 2437
Aor13HI	TCCGGA	1 cut(s)	129
AoxI	GGCC	7 cut(s)	219, 740, 858, 974, 2259, 2344, 2552
ApaLI	GTGCAC	1 cut(s)	719
ApeKI	GCWGC	6 cut(s)	63, 66, 311, 1035, 2248, 2372
ApoI	RAATTY	5 cut(s)	553, 1849, 1952, 2100, 2674
ArsI	GACNNNNNNTTYG	2 cut(s)	308, 340
Asp700I	GAANNNNTTC	1 cut(s)	23
AspS9I	GGNCC	4 cut(s)	252, 858, 935, 2345
AsuC2I	CCSGG	5 cut(s)	233, 389, 925, 2036, 2245
AsuHPI	GGTGA	6 cut(s)	1117, 1214, 1285, 1748, 1961, 2165
AsuII	TTCGAA	1 cut(s)	95
AvaII	GGWCC	2 cut(s)	252, 935
BaeGI	GKGCMC	1 cut(s)	723
BalI	TGGCCA	2 cut(s)	742, 2261
BanI	GGYRCC	1 cut(s)	1925
BanII	GRGCYC	2 cut(s)	354, 1448
BbsI	GAAGAC	3 cut(s)	393, 1407, 2319
Bbv12I	GWGCWC	3 cut(s)	354, 723, 1337
BbvCI	CCTCAGC	2 cut(s)	422, 1500
BbvI	GCAGC	6 cut(s)	75, 78, 298, 1022, 2235, 2384
BceAI	ACGGC	3 cut(s)	1634, 2581, 2628
BciT130I	CCWGG	1 cut(s)	50
BciVI	GTATCC	1 cut(s)	2483
BclI	TGATCA	3 cut(s)	1750, 2206, 2767
BcnI	CCSGG	5 cut(s)	233, 389, 925, 2036, 2245
BcoDI	GTCTC	6 cut(s)	453, 1283, 1366, 2084, 2231, 2729
BcuI	ACTAGT	1 cut(s)	2111
BfaI	CTAG	8 cut(s)	267, 618, 699, 846, 1563, 1880, 2112, 2136
BfmI	CTRYAG	1 cut(s)	2376
BfuAI	ACCTGC	1 cut(s)	1564
BfuI	GTATCC	1 cut(s)	2483
BisI	GCNGC	8 cut(s)	64, 67, 312, 816, 1036, 1647, 2249, 2373
BlpI	GCTNAGC	1 cut(s)	891
BlsI	GCNGC	8 cut(s)	65, 68, 313, 817, 1037, 1648, 2250, 2374
Bme1390I	CCNGG	6 cut(s)	50, 233, 389, 925, 2036, 2245
Bme18I	GGWCC	2 cut(s)	252, 935
BmeRI	GACNNNNNGTC	1 cut(s)	211
BmgT120I	GGNCC	4 cut(s)	252, 858, 935, 2345
BmiI	GGNNCC	8 cut(s)	272, 859, 936, 937, 1456, 1927, 2181, 2347
BmrFI	CCNGG	6 cut(s)	50, 233, 389, 925, 2036, 2245
BmrI	ACTGGG	2 cut(s)	864, 1101
BmsI	GCATC	6 cut(s)	910, 1055, 1227, 1879, 2209, 2527
BmuI	ACTGGG	2 cut(s)	864, 1101
BpiI	GAAGAC	3 cut(s)	393, 1407, 2319
BplI	GAGNNNNNCTC	2 cut(s)	1346, 1378
BpmI	CTGGAG	3 cut(s)	185, 1272, 2138
Bpu10I	CCTNAGC	4 cut(s)	152, 348, 422, 1500
Bpu1102I	GCTNAGC	1 cut(s)	891
Bpu14I	TTCGAA	1 cut(s)	95
BpuEI	CTTGAG	2 cut(s)	2108, 2621
BpuMI	CCSGG	5 cut(s)	233, 389, 925, 2036, 2245
Bsa29I	ATCGAT	1 cut(s)	414
BsaAI	YACGTR	1 cut(s)	2074
BsaBI	GATNNNNATC	1 cut(s)	2766
BsaHI	GRCGYC	2 cut(s)	105, 1644
BsaI	GGTCTC	1 cut(s)	2231
BsaJI	CCNNGG	2 cut(s)	813, 2035
BsaWI	WCCGGW	3 cut(s)	129, 318, 835
BsaXI	ACNNNNNCTCC	2 cut(s)	2361, 2391
Bsc4I	CCNNNNNNNGG	7 cut(s)	238, 403, 880, 2036, 2244, 2437, 2550
Bse118I	RCCGGY	1 cut(s)	2196
Bse1I	ACTGG	5 cut(s)	397, 859, 891, 1030, 1096
Bse3DI	GCAATG	1 cut(s)	222
Bse8I	GATNNNNATC	1 cut(s)	2766
BseAI	TCCGGA	1 cut(s)	129
BseBI	CCWGG	1 cut(s)	50
BseCI	ATCGAT	1 cut(s)	414
BseDI	CCNNGG	2 cut(s)	813, 2035
BseGI	GGATG	8 cut(s)	342, 690, 885, 1070, 1186, 1285, 1330, 2128
BseJI	GATNNNNATC	1 cut(s)	2766
BseLI	CCNNNNNNNGG	7 cut(s)	238, 403, 880, 2036, 2244, 2437, 2550
BseMI	GCAATG	1 cut(s)	222
BseNI	ACTGG	5 cut(s)	397, 859, 891, 1030, 1096
BseRI	GAGGAG	3 cut(s)	446, 811, 1488
BseSI	GKGCMC	1 cut(s)	723
BseXI	GCAGC	6 cut(s)	75, 78, 298, 1022, 2235, 2384
BseYI	CCCAGC	1 cut(s)	279
BsgI	GTGCAG	2 cut(s)	491, 860
Bsh1236I	CGCG	1 cut(s)	815
BshFI	GGCC	7 cut(s)	221, 742, 860, 976, 2261, 2346, 2554
BshNI	GGYRCC	1 cut(s)	1925
BshVI	ATCGAT	1 cut(s)	414
BsiHKAI	GWGCWC	3 cut(s)	354, 723, 1337
BslFI	GGGAC	4 cut(s)	248, 921, 1342, 1704
BslI	CCNNNNNNNGG	7 cut(s)	238, 403, 880, 2036, 2244, 2437, 2550
BsmAI	GTCTC	6 cut(s)	453, 1283, 1366, 2084, 2231, 2729
BsmBI	CGTCTC	1 cut(s)	2729
BsmFI	GGGAC	4 cut(s)	248, 921, 1342, 1704
BsmI	GAATGC	1 cut(s)	1244
BsnI	GGCC	7 cut(s)	221, 742, 860, 976, 2261, 2346, 2554
Bso31I	GGTCTC	1 cut(s)	2231
Bsp119I	TTCGAA	1 cut(s)	95
Bsp1286I	GDGCHC	4 cut(s)	354, 723, 1337, 1448
Bsp13I	TCCGGA	1 cut(s)	129
Bsp1720I	GCTNAGC	1 cut(s)	891
BspACI	CCGC	9 cut(s)	283, 474, 762, 813, 815, 1367, 1647, 2060, 2133
BspANI	GGCC	7 cut(s)	221, 742, 860, 976, 2261, 2346, 2554
BspDI	ATCGAT	1 cut(s)	414
BspEI	TCCGGA	1 cut(s)	129
BspFNI	CGCG	1 cut(s)	815
BspHI	TCATGA	1 cut(s)	1843
BspLI	GGNNCC	8 cut(s)	272, 859, 936, 937, 1456, 1927, 2181, 2347
BspMI	ACCTGC	1 cut(s)	1564
BspPI	GGATC	5 cut(s)	1013, 1308, 1762, 1897, 2311
BspQI	GCTCTTC	1 cut(s)	53
BspT104I	TTCGAA	1 cut(s)	95
BspT107I	GGYRCC	1 cut(s)	1925
BspTNI	GGTCTC	1 cut(s)	2231
BsrDI	GCAATG	1 cut(s)	222
BsrFI	RCCGGY	1 cut(s)	2196
BsrI	ACTGG	5 cut(s)	397, 859, 891, 1030, 1096
BssAI	RCCGGY	1 cut(s)	2196
BssECI	CCNNGG	2 cut(s)	813, 2035
BssNI	GRCGYC	2 cut(s)	105, 1644
Bst2UI	CCWGG	1 cut(s)	50
Bst4CI	ACNGT	7 cut(s)	15, 252, 598, 725, 963, 1679, 2719
Bst6I	CTCTTC	7 cut(s)	53, 197, 788, 1376, 1930, 2010, 2121
BstACI	GRCGYC	2 cut(s)	105, 1644
BstBAI	YACGTR	1 cut(s)	2074
BstBI	TTCGAA	1 cut(s)	95
BstC8I	GCNNGC	6 cut(s)	171, 264, 1242, 2198, 2439, 2552
BstDSI	CCRYGG	1 cut(s)	813
BstF5I	GGATG	8 cut(s)	342, 690, 885, 1070, 1186, 1285, 1330, 2128
BstFNI	CGCG	1 cut(s)	815
BstMAI	GTCTC	6 cut(s)	453, 1283, 1366, 2084, 2231, 2729
BstMWI	GCNNNNNNNGC	7 cut(s)	268, 311, 695, 973, 1231, 2378, 2669
BstNI	CCWGG	1 cut(s)	50
BstNSI	RCATGY	2 cut(s)	911, 1719
BstSCI	CCNGG	6 cut(s)	48, 231, 387, 923, 2034, 2243
BstSFI	CTRYAG	1 cut(s)	2376
BstSLI	GKGCMC	1 cut(s)	723
BstUI	CGCG	1 cut(s)	815
BstV1I	GCAGC	6 cut(s)	75, 78, 298, 1022, 2235, 2384
BstV2I	GAAGAC	3 cut(s)	393, 1407, 2319
BstX2I	RGATCY	1 cut(s)	1767
BstXI	CCANNNNNNTGG	1 cut(s)	946
BstYI	RGATCY	1 cut(s)	1767
Bsu15I	ATCGAT	1 cut(s)	414
BsuI	GTATCC	1 cut(s)	2483
BsuRI	GGCC	7 cut(s)	221, 742, 860, 976, 2261, 2346, 2554
BsuTUI	ATCGAT	1 cut(s)	414
BtgI	CCRYGG	1 cut(s)	813
BtsCI	GGATG	8 cut(s)	342, 690, 885, 1070, 1186, 1285, 1330, 2128
BtsI	GCAGTG	3 cut(s)	31, 300, 503
BveI	ACCTGC	1 cut(s)	1564
Cac8I	GCNNGC	6 cut(s)	171, 264, 1242, 2198, 2439, 2552
CaiI	CAGNNNCTG	3 cut(s)	505, 1672, 2437
CciI	TCATGA	1 cut(s)	1843
Cfr10I	RCCGGY	1 cut(s)	2196
Cfr13I	GGNCC	4 cut(s)	252, 858, 935, 2345
Cfr42I	CCGCGG	1 cut(s)	816
ClaI	ATCGAT	1 cut(s)	414
CseI	GACGC	2 cut(s)	113, 1652
Csp6I	GTAC	3 cut(s)	385, 493, 2594
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	3 cut(s)	385, 493, 2594
DraI	TTTAAA	1 cut(s)	736
DraIII	CACNNNGTG	1 cut(s)	2465
DrdI	GACNNNNNNGTC	1 cut(s)	1649
DriI	GACNNNNNGTC	1 cut(s)	211
DseDI	GACNNNNNNGTC	1 cut(s)	1649
EaeI	YGGCCR	2 cut(s)	740, 2259
Eam1104I	CTCTTC	7 cut(s)	53, 197, 788, 1376, 1930, 2010, 2121
Eam1105I	GACNNNNNGTC	1 cut(s)	211
EarI	CTCTTC	7 cut(s)	53, 197, 788, 1376, 1930, 2010, 2121
Ecl136II	GAGCTC	1 cut(s)	352
Eco24I	GRGCYC	2 cut(s)	354, 1448
Eco31I	GGTCTC	1 cut(s)	2231
Eco47I	GGWCC	2 cut(s)	252, 935
Eco53kI	GAGCTC	1 cut(s)	352
Eco57I	CTGAAG	3 cut(s)	482, 1378, 1965
EcoICRI	GAGCTC	1 cut(s)	352
EcoO109I	RGGNCCY	1 cut(s)	935
EcoRII	CCWGG	1 cut(s)	48
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	354, 1448
Esp3I	CGTCTC	1 cut(s)	2729
FalI	AAGNNNNNCTT	2 cut(s)	2035, 2067
FaqI	GGGAC	4 cut(s)	248, 921, 1342, 1704
FauI	CCCGC	2 cut(s)	276, 1360
FauNDI	CATATG	1 cut(s)	2149
FbaI	TGATCA	3 cut(s)	1750, 2206, 2767
Fnu4HI	GCNGC	8 cut(s)	64, 67, 312, 816, 1036, 1647, 2249, 2373
FokI	GGATG	8 cut(s)	329, 697, 892, 1077, 1193, 1292, 1337, 2135
FriOI	GRGCYC	2 cut(s)	354, 1448
Fsp4HI	GCNGC	8 cut(s)	64, 67, 312, 816, 1036, 1647, 2249, 2373
FspBI	CTAG	8 cut(s)	267, 618, 699, 846, 1563, 1880, 2112, 2136
GluI	GCNGC	8 cut(s)	64, 67, 312, 816, 1036, 1647, 2249, 2373
GsaI	CCCAGC	1 cut(s)	283
GsuI	CTGGAG	3 cut(s)	185, 1272, 2138
HaeIII	GGCC	7 cut(s)	221, 742, 860, 976, 2261, 2346, 2554
HgaI	GACGC	2 cut(s)	113, 1652
Hin1I	GRCGYC	2 cut(s)	105, 1644
HincII	GTYRAC	3 cut(s)	1089, 2335, 2715
HindII	GTYRAC	3 cut(s)	1089, 2335, 2715
HindIII	AAGCTT	2 cut(s)	134, 171
HphI	GGTGA	6 cut(s)	1117, 1214, 1285, 1748, 1961, 2165
Hpy166II	GTNNAC	6 cut(s)	366, 721, 1089, 2335, 2650, 2715
Hpy8I	GTNNAC	6 cut(s)	366, 721, 1089, 2335, 2650, 2715
Hpy99I	CGWCG	1 cut(s)	107
HpyCH4III	ACNGT	7 cut(s)	15, 252, 598, 725, 963, 1679, 2719
HpyCH4IV	ACGT	2 cut(s)	2073, 2219
HpyF10VI	GCNNNNNNNGC	7 cut(s)	268, 311, 695, 973, 1231, 2378, 2669
HpySE526I	ACGT	2 cut(s)	2073, 2219
Hsp92I	GRCGYC	2 cut(s)	105, 1644
KflI	GGGWCCC	1 cut(s)	935
Kpn2I	TCCGGA	1 cut(s)	129
KroI	GCCGGC	1 cut(s)	2196
KroNI	GCCGGC	1 cut(s)	2198
Ksp22I	TGATCA	3 cut(s)	1750, 2206, 2767
KspI	CCGCGG	1 cut(s)	816
LguI	GCTCTTC	1 cut(s)	53
LmnI	GCTCC	8 cut(s)	166, 276, 357, 1253, 1372, 1669, 2144, 2369
Lsp1109I	GCAGC	6 cut(s)	75, 78, 298, 1022, 2235, 2384
LweI	GCATC	6 cut(s)	910, 1055, 1227, 1879, 2209, 2527
MaeI	CTAG	8 cut(s)	267, 618, 699, 846, 1563, 1880, 2112, 2136
MaeII	ACGT	2 cut(s)	2073, 2219
MaeIII	GTNAC	6 cut(s)	850, 1189, 2272, 2480, 2608, 2746
MfeI	CAATTG	2 cut(s)	901, 2263
MflI	RGATCY	1 cut(s)	1767
MhlI	GDGCHC	4 cut(s)	354, 723, 1337, 1448
MlsI	TGGCCA	2 cut(s)	742, 2261
MluNI	TGGCCA	2 cut(s)	742, 2261
MlyI	GAGTC	5 cut(s)	221, 338, 1393, 1616, 2138
MmeI	TCCRAC	5 cut(s)	69, 126, 1046, 1482, 2711
Mox20I	TGGCCA	2 cut(s)	742, 2261
Mph1103I	ATGCAT	1 cut(s)	6
MroI	TCCGGA	1 cut(s)	129
MroNI	GCCGGC	1 cut(s)	2196
MroXI	GAANNNNTTC	1 cut(s)	23
MscI	TGGCCA	2 cut(s)	742, 2261
MseI	TTAA	9 cut(s)	735, 789, 1047, 1476, 1818, 2214, 2385, 2682, 2776
MslI	CAYNNNNRTG	1 cut(s)	1758
Msp20I	TGGCCA	2 cut(s)	742, 2261
MspA1I	CMGCKG	6 cut(s)	283, 421, 764, 815, 1367, 2375
MspR9I	CCNGG	6 cut(s)	50, 233, 389, 925, 2036, 2245
MunI	CAATTG	2 cut(s)	901, 2263
Mva1269I	GAATGC	1 cut(s)	1244
MvaI	CCWGG	1 cut(s)	50
MvnI	CGCG	1 cut(s)	815
MwoI	GCNNNNNNNGC	7 cut(s)	268, 311, 695, 973, 1231, 2378, 2669
NaeI	GCCGGC	1 cut(s)	2198
NciI	CCSGG	5 cut(s)	233, 389, 925, 2036, 2245
NdeI	CATATG	1 cut(s)	2149
NgoMIV	GCCGGC	1 cut(s)	2196
NlaIV	GGNNCC	8 cut(s)	272, 859, 936, 937, 1456, 1927, 2181, 2347
NmeAIII	GCCGAG	1 cut(s)	1201
NmuCI	GTSAC	3 cut(s)	2272, 2480, 2608
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	911, 1719
NspV	TTCGAA	1 cut(s)	95
PagI	TCATGA	1 cut(s)	1843
PciSI	GCTCTTC	1 cut(s)	53
PctI	GAATGC	1 cut(s)	1244
PdiI	GCCGGC	1 cut(s)	2198
PdmI	GAANNNNTTC	1 cut(s)	23
PflFI	GACNNNGTC	1 cut(s)	1679
PflMI	CCANNNNNTGG	2 cut(s)	880, 2437
PfoI	TCCNGGA	1 cut(s)	48
PkrI	GCNGC	8 cut(s)	65, 68, 313, 817, 1037, 1648, 2250, 2374
PleI	GAGTC	5 cut(s)	220, 337, 1392, 1616, 2137
PpsI	GAGTC	5 cut(s)	220, 337, 1392, 1616, 2137
Ppu21I	YACGTR	1 cut(s)	2074
PpuMI	RGGWCCY	1 cut(s)	935
Psp124BI	GAGCTC	1 cut(s)	354
Psp5II	RGGWCCY	1 cut(s)	935
Psp6I	CCWGG	1 cut(s)	48
PspFI	CCCAGC	1 cut(s)	279
PspGI	CCWGG	1 cut(s)	48
PspN4I	GGNNCC	8 cut(s)	272, 859, 936, 937, 1456, 1927, 2181, 2347
PspPI	GGNCC	4 cut(s)	252, 858, 935, 2345
PspPPI	RGGWCCY	1 cut(s)	935
PstNI	CAGNNNCTG	3 cut(s)	505, 1672, 2437
PsuI	RGATCY	1 cut(s)	1767
PsyI	GACNNNGTC	1 cut(s)	1679
PvuII	CAGCTG	2 cut(s)	421, 2375
RsaI	GTAC	3 cut(s)	386, 494, 2595
RsaNI	GTAC	3 cut(s)	385, 493, 2594
RseI	CAYNNNNRTG	1 cut(s)	1758
SacI	GAGCTC	1 cut(s)	354
SacII	CCGCGG	1 cut(s)	816
SapI	GCTCTTC	1 cut(s)	53
SaqAI	TTAA	9 cut(s)	735, 789, 1047, 1476, 1818, 2214, 2385, 2682, 2776
SatI	GCNGC	8 cut(s)	64, 67, 312, 816, 1036, 1647, 2249, 2373
Sau96I	GGNCC	4 cut(s)	252, 858, 935, 2345
SchI	GAGTC	5 cut(s)	221, 338, 1393, 1616, 2138
ScrFI	CCNGG	6 cut(s)	50, 233, 389, 925, 2036, 2245
SduI	GDGCHC	4 cut(s)	354, 723, 1337, 1448
SfaNI	GCATC	6 cut(s)	910, 1055, 1227, 1879, 2209, 2527
SfcI	CTRYAG	1 cut(s)	2376
Sfr303I	CCGCGG	1 cut(s)	816
SfuI	TTCGAA	1 cut(s)	95
SgrBI	CCGCGG	1 cut(s)	816
SinI	GGWCC	2 cut(s)	252, 935
SmiMI	CAYNNNNRTG	1 cut(s)	1758
SmlI	CTYRAG	2 cut(s)	2087, 2600
SmoI	CTYRAG	2 cut(s)	2087, 2600
SpeI	ACTAGT	1 cut(s)	2111
SsiI	CCGC	9 cut(s)	283, 474, 762, 813, 815, 1367, 1647, 2060, 2133
SspMI	CTAG	8 cut(s)	267, 618, 699, 846, 1563, 1880, 2112, 2136
SstI	GAGCTC	1 cut(s)	354
StyD4I	CCNGG	6 cut(s)	48, 231, 387, 923, 2034, 2243
TaaI	ACNGT	7 cut(s)	15, 252, 598, 725, 963, 1679, 2719
TaiI	ACGT	2 cut(s)	2076, 2222
TaqI	TCGA	5 cut(s)	95, 327, 414, 785, 1791
TauI	GCSGC	2 cut(s)	818, 1649
Tru1I	TTAA	9 cut(s)	735, 789, 1047, 1476, 1818, 2214, 2385, 2682, 2776
Tru9I	TTAA	9 cut(s)	735, 789, 1047, 1476, 1818, 2214, 2385, 2682, 2776
TseFI	GTSAC	3 cut(s)	2272, 2480, 2608
TseI	GCWGC	6 cut(s)	63, 66, 311, 1035, 2248, 2372
Tsp45I	GTSAC	3 cut(s)	2272, 2480, 2608
TspDTI	ATGAA	8 cut(s)	372, 1200, 1394, 1398, 1710, 1860, 2139, 2325
Tth111I	GACNNNGTC	1 cut(s)	1679
Van91I	CCANNNNNTGG	2 cut(s)	880, 2437
VneI	GTGCAC	1 cut(s)	719
VpaK11BI	GGWCC	2 cut(s)	252, 935
XapI	RAATTY	5 cut(s)	553, 1849, 1952, 2100, 2674
XbaI	TCTAGA	1 cut(s)	845
XceI	RCATGY	2 cut(s)	911, 1719
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1778
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	8 cut(s)	267, 618, 699, 846, 1563, 1880, 2112, 2136
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.