pycom02g12670
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004440
Physical Location & Seq
Reverse (-)
9257298 .. 9258055
758 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12670.1

Sequence Viewer

Length: 687 bp
ATGAGAGAAATTTCTGCAACTAGTTTGGAGGATGAAGAGTCCGCTAGTGATGGAGCATATGGTGATGGGCAAGACTTGGACAGAAGGGAACCTGAGATAATATGTCGGCATCTTGATCATGTTAATACGTTAGGTCTCAATAAAACCACCTCCCGGCTGCGATTTTTGGTCAATTGGCTTGTGACCTCTTTGCTACAACTAAGGTTGCGTGGATCAGATGAAGACAAAGGAACAAATCTTGTTGACATCTATGGCCCAAAAATAAAATACGAAAGGGGAGCAGCTGTAGCTTTTAATGTGAAAGACCAGAATAGAGGGCTAATCAATCCAGAAGTTGTCCAGAAGCTGGCTGAAAAAGAAGGAATCGCACTTGGCGTTGGTATCCTCAGTCACATACGAATCATGGATAGTCCAAGACAGAAAAATGGTGTTTTGAATCTTCAAGATGCTACCCTTTGCAGGCCAATGGAGAACGGCAGAAATGGTAAAAAAAGCGAGTTTGTACGCCTTGAGGTTGTGACAGCTTCTCTGGGATTCCTTACCAACTTTGAAGATGTTTACAAGTTATGGGCTTTTATAGCAAATTTTCTTAATCCAACCTTTATCAGAGAGGGTGGGCTGTCAACTGTCTATGAAGAATCTGAGACGAAATCTGTAACATCTGTCCATGATATTGTTCAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.5

Weight (kDa)

5.5

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 346
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 220
AcsI RAATTY 2 cut(s) 9, 583
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 3 cut(s) 153, 346, 459
AgsI TTSAA 4 cut(s) 436, 443, 551, 680
AhlI ACTAGT 1 cut(s) 20
AluBI AGCT 4 cut(s) 284, 290, 346, 524
AluI AGCT 4 cut(s) 284, 290, 346, 524
Alw26I GTCTC 2 cut(s) 140, 638
AlwI GGATC 1 cut(s) 220
AlwNI CAGNNNCTG 1 cut(s) 346
AoxI GGCC 2 cut(s) 253, 461
ApeKI GCWGC 2 cut(s) 157, 281
ApoI RAATTY 2 cut(s) 9, 583
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 144, 293
BccI CCATC 2 cut(s) 44, 59
BceAI ACGGC 1 cut(s) 490
BciVI GTATCC 1 cut(s) 392
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 140, 638
BcuI ACTAGT 1 cut(s) 20
BfaI CTAG 2 cut(s) 21, 45
BfmI CTRYAG 1 cut(s) 285
BfuI GTATCC 1 cut(s) 392
BisI GCNGC 2 cut(s) 158, 282
BlsI GCNGC 2 cut(s) 159, 283
Bme1390I CCNGG 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 118, 436
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 154
BsaI GGTCTC 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 346, 459
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 3 cut(s) 153, 346, 459
BseMII CTCAG 3 cut(s) 84, 400, 633
BseXI GCAGC 2 cut(s) 144, 293
BshFI GGCC 2 cut(s) 255, 463
BsiSI CCGG 1 cut(s) 154
BslI CCNNNNNNNGG 3 cut(s) 153, 346, 459
BsmAI GTCTC 2 cut(s) 140, 638
BsmBI CGTCTC 1 cut(s) 638
BsnI GGCC 2 cut(s) 255, 463
Bso31I GGTCTC 1 cut(s) 140
Bsp143I GATC 2 cut(s) 115, 212
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 255, 463
BspCNI CTCAG 3 cut(s) 85, 399, 634
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 1 cut(s) 220
BspTNI GGTCTC 1 cut(s) 140
BssMI GATC 2 cut(s) 115, 212
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 348, 461
BstDEI CTNAG 4 cut(s) 93, 200, 386, 642
BstF5I GGATG 1 cut(s) 37
BstKTI GATC 2 cut(s) 118, 215
BstMAI GTCTC 2 cut(s) 140, 638
BstMBI GATC 2 cut(s) 115, 212
BstMWI GCNNNNNNNGC 2 cut(s) 287, 578
BstSCI CCNGG 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 144, 293
BstV2I GAAGAC 1 cut(s) 228
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 255, 463
BtsCI GGATG 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 348, 461
CaiI CAGNNNCTG 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 503
CviAII CATG 3 cut(s) 119, 403, 668
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 4 cut(s) 93, 200, 386, 642
DpnI GATC 2 cut(s) 117, 214
DpnII GATC 2 cut(s) 115, 212
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 638
FaeI CATG 3 cut(s) 122, 406, 671
FatI CATG 3 cut(s) 118, 402, 667
FauNDI CATATG 1 cut(s) 58
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 158, 282
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 158, 282
FspBI CTAG 2 cut(s) 21, 45
GluI GCNGC 2 cut(s) 158, 282
HaeIII GGCC 2 cut(s) 255, 463
HapII CCGG 1 cut(s) 154
Hin1II CATG 3 cut(s) 122, 406, 671
HincII GTYRAC 2 cut(s) 244, 624
HindII GTYRAC 2 cut(s) 244, 624
HinfI GANTC 6 cut(s) 38, 363, 399, 436, 534, 638
HpaII CCGG 1 cut(s) 154
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 244, 559, 624
Hpy188I TCNGA 3 cut(s) 217, 608, 643
Hpy188III TCNNGA 4 cut(s) 113, 329, 340, 443
Hpy8I GTNNAC 3 cut(s) 244, 559, 624
HpyAV CCTTC 2 cut(s) 78, 353
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 17, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 578
HpyF3I CTNAG 4 cut(s) 93, 200, 386, 642
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 3 cut(s) 122, 406, 671
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 115, 212
LmnI GCTCC 2 cut(s) 53, 278
LpnPI CCDG 8 cut(s) 105, 167, 320, 332, 342, 353, 445, 515
Lsp1109I GCAGC 2 cut(s) 144, 293
LweI GCATC 2 cut(s) 118, 436
MaeI CTAG 2 cut(s) 21, 45
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 4 cut(s) 181, 389, 517, 655
MalI GATC 2 cut(s) 117, 214
MboI GATC 2 cut(s) 115, 212
MboII GAAGA 5 cut(s) 47, 233, 431, 563, 647
MfeI CAATTG 1 cut(s) 172
MluCI AATT 3 cut(s) 9, 172, 583
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 620
MnlI CCTC 7 cut(s) 22, 160, 196, 308, 395, 505, 604
MseI TTAA 3 cut(s) 123, 294, 591
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 154
MunI CAATTG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 287, 578
NciI CCSGG 1 cut(s) 154
NdeI CATATG 1 cut(s) 58
NdeII GATC 2 cut(s) 115, 212
NlaIII CATG 3 cut(s) 122, 406, 671
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 3 cut(s) 181, 389, 517
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PfeI GAWTC 5 cut(s) 363, 399, 436, 534, 638
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 2 cut(s) 159, 283
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
SaqAI TTAA 3 cut(s) 123, 294, 591
SatI GCNGC 2 cut(s) 158, 282
Sau3AI GATC 2 cut(s) 115, 212
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 154
SfaNI GCATC 2 cut(s) 118, 436
SfcI CTRYAG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SpeI ACTAGT 1 cut(s) 20
Sse9I AATT 3 cut(s) 9, 172, 583
SsiI CCGC 1 cut(s) 42
SspMI CTAG 2 cut(s) 21, 45
StyD4I CCNGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 1 cut(s) 131
TasI AATT 3 cut(s) 9, 172, 583
TfiI GAWTC 5 cut(s) 363, 399, 436, 534, 638
Tru1I TTAA 3 cut(s) 123, 294, 591
Tru9I TTAA 3 cut(s) 123, 294, 591
TseFI GTSAC 3 cut(s) 181, 389, 517
TseI GCWGC 2 cut(s) 157, 281
Tsp45I GTSAC 3 cut(s) 181, 389, 517
TspDTI ATGAA 3 cut(s) 48, 234, 648
Van91I CCANNNNNTGG 1 cut(s) 346
XapI RAATTY 2 cut(s) 9, 583
XspI CTAG 2 cut(s) 21, 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.