Rorug02G0116600
ERF Family

superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004440
Physical Location & Seq
Reverse (-)
9895282 .. 9897511
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0116600.1

Sequence Viewer

Length: 1266 bp
ATGTCACTCTCTTCAGCTCTAAACCCCCAAACCTCCAAGCTCTTCTCTCTCCTCTCTTTCACACCCCACAAGCTCTCACCACCCACCTCCCTGAAATTCTCATCCAAACTCCACACCCTCTCACCCTCCTTCTCCTTCAACTCCTCTCACTCCACCTTCGAAGAAGACCACGTCATCGGCGACTGCGTCGTCTTCGAAGAGGGCGTGTTCGACGACCCGTATCTTCAAAACGACACCGAAATCGAACATTTCAACCCGGAAAAGCCGAAGCCCAGAAAACGCGTTGAAATTGAGACTGAGAATTTGGTGCCGGAGAAATGGAAGGAGGTGCAGGCCACAGTGAACATAACAAAGAAGGAGAGGAGGAAGATTGCGCAGGAGTTGGAGTTCGGTTCGAGGGTCGAGAAGAACAAGAGGGGGTTGGAGCCCATAAGGAATTTGAATTTGGAGGAGTACTCGGCGTATAGAGAGGCCAAGTTGAACCAGCTGAAGCCACTGGTTTTGGATAGGCCTGACAAGTTTAAGGAGGAGGGTGAAGAGTGTGAAGGTGGTGATGGGTTTGTGAGTGAGAGAGTGGCGCCTAAGAATCCCAGGTGGGCTGTTTATGGGAAGGGTTTGGACGATGTTACTGAGTTCTTTAACAGTGGGCATTATGAGCCTGGTGTGAACAAATCTGAAGGACCCCGGCCTTTGTTTACCAAAGAGGAAAAGCTGATGCTGAATAGGCGAAAACCTGATATAGCTGCTGCCACTTCTAGTAAATGGCTGCCCCTTCACACTCTTGCCGCATCAGGAGAATATTACCTTATGGATGCTTTATTGAAACATGAAGCTGATATCAATGCTGTGGATAAGTTCAATGTCTTTCAGGATGATTGGACTGTTCTTCACAAAGCAATTATTGGTAAAAACCAGGCCATTACAAACTATCTTCTCAGAGAATCGGCTAATCCATTTGTTCGTGATAAAGATGGCGCTACCTTGATGCATTATGCTGTCCGGACAGCTTCAAGTCAAGCAATTAAGATTCTTCTGTTATACAACGTTGATATAAACCTTCAGGACAATGATGGGTGGACGCCCTTACATCTAGCTGTTCAAGGCCGAAGAACAGATGTAGTGAGGCTTTTGTTGATTAAAGGCGCGGATAAGACATTGAAAAACAAGGATGGATTAACCCCTCTTGAGCTTTGCCTCTATTCTGGCCGAGACACGAGGACTTACGAGCTGATTAAGCTATTGAAGCTTCTTCCCAAAACCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

47.97

Weight (kDa)

7.19

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 250 - 321 5.6e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 258 - 355 1.3e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 308 - 388 1.9e-16 Ankyrin repeats (3 copies)
Ank_5 PF13857 315 - 365 2.8e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 329 - 403 3.3e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 340 - 378 1.8e-07 Ankyrin repeats (many copies)
Ank PF00023 357 - 389 4e-08 Ankyrin repeat
Ank_5 PF13857 360 - 398 7.4e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
Acc16I TGCGCA 1 cut(s) 375
AccB1I GGYRCC 2 cut(s) 307, 577
AccII CGCG 2 cut(s) 282, 1145
AccIII TCCGGA 1 cut(s) 999
AciI CCGC 2 cut(s) 786, 1145
AclI AACGTT 1 cut(s) 1044
AcoI YGGCCR 1 cut(s) 1204
AcsI RAATTY 4 cut(s) 95, 301, 436, 442
AcuI CTGAAG 3 cut(s) 509, 696, 1043
AcyI GRCGYC 2 cut(s) 578, 1079
AfaI GTAC 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 280
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 3 cut(s) 590, 658, 912
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
Alw26I GTCTC 2 cut(s) 287, 1203
Aor13HI TCCGGA 1 cut(s) 999
AoxI GGCC 7 cut(s) 333, 471, 509, 686, 915, 1102, 1204
ApeKI GCWGC 3 cut(s) 743, 746, 766
ApoI RAATTY 4 cut(s) 95, 301, 436, 442
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
AspLEI GCGC 4 cut(s) 376, 580, 977, 1145
AspS9I GGNCC 1 cut(s) 680
AsuC2I CCSGG 2 cut(s) 257, 685
AsuHPI GGTGA 4 cut(s) 69, 114, 545, 563
AsuII TTCGAA 2 cut(s) 159, 195
AvaII GGWCC 1 cut(s) 680
BanI GGYRCC 2 cut(s) 307, 577
BanII GRGCYC 1 cut(s) 429
BauI CACGAG 1 cut(s) 1213
BbsI GAAGAC 2 cut(s) 171, 184
BbvI GCAGC 3 cut(s) 730, 733, 753
BccI CCATC 4 cut(s) 548, 965, 1064, 1163
BciT130I CCWGG 3 cut(s) 592, 660, 914
BcnI CCSGG 2 cut(s) 257, 685
BcoDI GTCTC 2 cut(s) 287, 1203
BfaI CTAG 2 cut(s) 756, 1091
BfoI RGCGCY 2 cut(s) 581, 978
BisI GCNGC 4 cut(s) 744, 747, 767, 786
BlsI GCNGC 4 cut(s) 745, 748, 768, 787
BmcAI AGTACT 1 cut(s) 455
Bme1390I CCNGG 5 cut(s) 257, 592, 660, 685, 914
Bme18I GGWCC 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 4 cut(s) 309, 426, 579, 682
BmrFI CCNGG 5 cut(s) 257, 592, 660, 685, 914
BmsI GCATC 4 cut(s) 705, 797, 802, 975
BpiI GAAGAC 2 cut(s) 171, 184
BplI GAGNNNNNCTC 2 cut(s) 440, 472
Bpu14I TTCGAA 2 cut(s) 159, 195
BpuEI CTTGAG 1 cut(s) 1205
BpuMI CCSGG 2 cut(s) 257, 685
BsaHI GRCGYC 2 cut(s) 578, 1079
BsaJI CCNNGG 2 cut(s) 590, 683
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bse1I ACTGG 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 999
BseBI CCWGG 3 cut(s) 592, 660, 914
BseDI CCNNGG 2 cut(s) 590, 683
BseGI GGATG 4 cut(s) 101, 817, 877, 1174
BseMII CTCAG 3 cut(s) 288, 621, 949
BseNI ACTGG 1 cut(s) 501
BseRI GAGGAG 5 cut(s) 41, 133, 376, 464, 542
BseXI GCAGC 3 cut(s) 730, 733, 753
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 282, 1145
BshFI GGCC 7 cut(s) 335, 473, 511, 688, 917, 1104, 1206
BshNI GGYRCC 2 cut(s) 307, 577
BsiSI CCGG 4 cut(s) 257, 311, 685, 1000
BsmAI GTCTC 2 cut(s) 287, 1203
BsnI GGCC 7 cut(s) 335, 473, 511, 688, 917, 1104, 1206
Bsp119I TTCGAA 2 cut(s) 159, 195
Bsp1286I GDGCHC 1 cut(s) 429
Bsp13I TCCGGA 1 cut(s) 999
BspACI CCGC 2 cut(s) 786, 1145
BspANI GGCC 7 cut(s) 335, 473, 511, 688, 917, 1104, 1206
BspCNI CTCAG 3 cut(s) 289, 622, 948
BspEI TCCGGA 1 cut(s) 999
BspFNI CGCG 2 cut(s) 282, 1145
BspLI GGNNCC 4 cut(s) 309, 426, 579, 682
BspQI GCTCTTC 1 cut(s) 47
BspT104I TTCGAA 2 cut(s) 159, 195
BspT107I GGYRCC 2 cut(s) 307, 577
BsrI ACTGG 1 cut(s) 501
BssECI CCNNGG 2 cut(s) 590, 683
BssNI GRCGYC 2 cut(s) 578, 1079
BssSI CACGAG 1 cut(s) 1213
Bst2BI CACGAG 1 cut(s) 1213
Bst2UI CCWGG 3 cut(s) 592, 660, 914
Bst4CI ACNGT 3 cut(s) 340, 644, 883
Bst6I CTCTTC 4 cut(s) 16, 47, 192, 531
BstACI GRCGYC 2 cut(s) 578, 1079
BstBI TTCGAA 2 cut(s) 159, 195
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 4 cut(s) 297, 582, 630, 935
BstF5I GGATG 4 cut(s) 101, 817, 877, 1174
BstFNI CGCG 2 cut(s) 282, 1145
BstH2I RGCGCY 2 cut(s) 581, 978
BstHHI GCGC 4 cut(s) 376, 580, 977, 1145
BstMAI GTCTC 2 cut(s) 287, 1203
BstMWI GCNNNNNNNGC 4 cut(s) 655, 724, 1234, 1243
BstNI CCWGG 3 cut(s) 592, 660, 914
BstSCI CCNGG 5 cut(s) 255, 590, 658, 683, 912
BstUI CGCG 2 cut(s) 282, 1145
BstV1I GCAGC 3 cut(s) 730, 733, 753
BstV2I GAAGAC 2 cut(s) 171, 184
BsuRI GGCC 7 cut(s) 335, 473, 511, 688, 917, 1104, 1206
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 4 cut(s) 101, 817, 877, 1174
BtsIMutI CAGTG 3 cut(s) 345, 494, 649
Cac8I GCNNGC 1 cut(s) 333
CfoI GCGC 4 cut(s) 376, 580, 977, 1145
Cfr13I GGNCC 1 cut(s) 680
CseI GACGC 2 cut(s) 175, 1087
Csp6I GTAC 1 cut(s) 454
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviAII CATG 1 cut(s) 827
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 4 cut(s) 297, 582, 630, 935
DinI GGCGCC 1 cut(s) 579
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 1204
Eam1104I CTCTTC 4 cut(s) 16, 47, 192, 531
EarI CTCTTC 4 cut(s) 16, 47, 192, 531
Eco147I AGGCCT 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 680
Eco57I CTGAAG 3 cut(s) 509, 696, 1043
EcoO109I RGGNCCY 1 cut(s) 680
EcoRII CCWGG 3 cut(s) 590, 658, 912
EcoRV GATATC 1 cut(s) 838
EcoT22I ATGCAT 1 cut(s) 990
EcoT38I GRGCYC 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 579
EheI GGCGCC 1 cut(s) 579
FaeI CATG 1 cut(s) 830
FatI CATG 1 cut(s) 826
Fnu4HI GCNGC 4 cut(s) 744, 747, 767, 786
FokI GGATG 4 cut(s) 88, 824, 884, 1181
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 4 cut(s) 744, 747, 767, 786
FspBI CTAG 2 cut(s) 756, 1091
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 4 cut(s) 375, 579, 976, 1144
GluI GCNGC 4 cut(s) 744, 747, 767, 786
HaeII RGCGCY 2 cut(s) 581, 978
HaeIII GGCC 7 cut(s) 335, 473, 511, 688, 917, 1104, 1206
HapII CCGG 4 cut(s) 257, 311, 685, 1000
HgaI GACGC 2 cut(s) 175, 1087
HhaI GCGC 4 cut(s) 376, 580, 977, 1145
Hin1I GRCGYC 2 cut(s) 578, 1079
Hin1II CATG 1 cut(s) 830
Hin6I GCGC 4 cut(s) 374, 578, 975, 1143
HinP1I GCGC 4 cut(s) 374, 578, 975, 1143
HindIII AAGCTT 1 cut(s) 1244
HinfI GANTC 3 cut(s) 586, 941, 1027
HpaII CCGG 4 cut(s) 257, 311, 685, 1000
HphI GGTGA 4 cut(s) 69, 114, 545, 563
Hpy166II GTNNAC 4 cut(s) 343, 667, 696, 1077
Hpy188I TCNGA 2 cut(s) 676, 938
Hpy188III TCNNGA 7 cut(s) 403, 792, 869, 962, 1000, 1061, 1184
Hpy8I GTNNAC 4 cut(s) 343, 667, 696, 1077
Hpy99I CGWCG 2 cut(s) 191, 215
HpyCH4III ACNGT 3 cut(s) 340, 644, 883
HpyCH4IV ACGT 2 cut(s) 171, 1044
HpyCH4V TGCA 2 cut(s) 331, 988
HpyF10VI GCNNNNNNNGC 4 cut(s) 655, 724, 1234, 1243
HpyF3I CTNAG 4 cut(s) 297, 582, 630, 935
HpySE526I ACGT 2 cut(s) 171, 1044
Hsp92I GRCGYC 2 cut(s) 578, 1079
Hsp92II CATG 1 cut(s) 830
HspAI GCGC 4 cut(s) 374, 578, 975, 1143
KasI GGCGCC 1 cut(s) 577
Kpn2I TCCGGA 1 cut(s) 999
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 3 cut(s) 730, 733, 753
LweI GCATC 4 cut(s) 705, 797, 802, 975
MaeI CTAG 2 cut(s) 756, 1091
MaeII ACGT 2 cut(s) 171, 1044
MaeIII GTNAC 2 cut(s) 3, 625
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 7 cut(s) 95, 288, 301, 436, 442, 897, 1020
MluI ACGCGT 1 cut(s) 280
Mly113I GGCGCC 1 cut(s) 578
MmeI TCCRAC 2 cut(s) 363, 402
Mph1103I ATGCAT 1 cut(s) 990
MroI TCCGGA 1 cut(s) 999
MseI TTAA 7 cut(s) 522, 639, 1023, 1137, 1175, 1233, 1264
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 4 cut(s) 257, 311, 685, 1000
MspR9I CCNGG 5 cut(s) 257, 592, 660, 685, 914
MvaI CCWGG 3 cut(s) 592, 660, 914
MvnI CGCG 2 cut(s) 282, 1145
MwoI GCNNNNNNNGC 4 cut(s) 655, 724, 1234, 1243
NarI GGCGCC 1 cut(s) 578
NciI CCSGG 2 cut(s) 257, 685
NlaIII CATG 1 cut(s) 830
NlaIV GGNNCC 4 cut(s) 309, 426, 579, 682
NmeAIII GCCGAG 2 cut(s) 437, 1232
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 375
NsiI ATGCAT 1 cut(s) 990
NspV TTCGAA 2 cut(s) 159, 195
PceI AGGCCT 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 183, 201
PfeI GAWTC 3 cut(s) 586, 941, 1027
PflFI GACNNNGTC 2 cut(s) 170, 185
PkrI GCNGC 4 cut(s) 745, 748, 768, 787
PluTI GGCGCC 1 cut(s) 581
PpuMI RGGWCCY 1 cut(s) 680
Psp1406I AACGTT 1 cut(s) 1044
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 3 cut(s) 590, 658, 912
PspGI CCWGG 3 cut(s) 590, 658, 912
PspN4I GGNNCC 4 cut(s) 309, 426, 579, 682
PspPI GGNCC 1 cut(s) 680
PspPPI RGGWCCY 1 cut(s) 680
PsyI GACNNNGTC 2 cut(s) 170, 185
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 7 cut(s) 522, 639, 1023, 1137, 1175, 1233, 1264
SatI GCNGC 4 cut(s) 744, 747, 767, 786
Sau96I GGNCC 1 cut(s) 680
ScaI AGTACT 1 cut(s) 455
ScrFI CCNGG 5 cut(s) 257, 592, 660, 685, 914
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 4 cut(s) 705, 797, 802, 975
SfoI GGCGCC 1 cut(s) 579
SfuI TTCGAA 2 cut(s) 159, 195
SinI GGWCC 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
Sse9I AATT 7 cut(s) 95, 288, 301, 436, 442, 897, 1020
SseBI AGGCCT 1 cut(s) 511
SsiI CCGC 2 cut(s) 786, 1145
SspDI GGCGCC 1 cut(s) 577
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 756, 1091
StuI AGGCCT 1 cut(s) 511
StyD4I CCNGG 5 cut(s) 255, 590, 658, 683, 912
TaaI ACNGT 3 cut(s) 340, 644, 883
TaiI ACGT 2 cut(s) 174, 1047
TaqI TCGA 6 cut(s) 159, 195, 210, 243, 395, 402
TasI AATT 7 cut(s) 95, 288, 301, 436, 442, 897, 1020
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 3 cut(s) 586, 941, 1027
Tru1I TTAA 7 cut(s) 522, 639, 1023, 1137, 1175, 1233, 1264
Tru9I TTAA 7 cut(s) 522, 639, 1023, 1137, 1175, 1233, 1264
TscAI CASTG 3 cut(s) 345, 501, 649
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 743, 746, 766
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 843
TspRI CASTG 3 cut(s) 345, 501, 649
Tth111I GACNNNGTC 2 cut(s) 170, 185
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 4 cut(s) 95, 301, 436, 442
XspI CTAG 2 cut(s) 756, 1091
ZrmI AGTACT 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.