MD02G1217700.v1.1

Ribosomal protein S19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009419
Physical Location & Seq
Forward (+)
25293864 .. 25295375
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1217700.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGCAAAGCGTCTAATTGCAACCCTCAATCGTGGCCTAGTTGGCAAACTCTTCTCCGAGTCCTCTACCAAGCTGAACTCCGGAAAGCCAGTGGTTGTGGGAACGTGTGGACTGGAAAAAGCATGCTATAGTACCCAAGCCAAGCAAGAATCTCATTTTTACGATGGCGCTTTTGTGGATGCATTCCTGATGAAAATGAAGAACAATAAAGATCTTCTTAACAATAAGAAAATTTGGTCACGCAGATCTACCATTTTGCCAGAATTTGTGGGTACTACAGTCCGTATCTATAATGGAAAAAATCATATTCGCTGCAAGATCACCGAAGAGAAGGTTGGTCATAAATTTGGAGAGTTTGCAATGACACGGAAACGAAGAATTCAGGCTAAAACTACTGCACCAGTAAGACCAGTGAAACCAGGAAAAAAAGCAGGCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S19

Protein Analysis

148

Amino Acids

16.5

Weight (kDa)

10.4

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S19 PF00203 56 - 126 7.2e-25 Ribosomal protein S19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 80
AcsI RAATTY 4 cut(s) 231, 263, 344, 378
AfaI GTAC 2 cut(s) 133, 274
AflIII ACRYGT 1 cut(s) 104
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 4 cut(s) 231, 263, 344, 378
AspLEI GCGC 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 313
BaeI ACNNNNGTAYC 2 cut(s) 264, 297
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 420
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 127, 276
BfoI RGCGCY 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 42
BglII AGATCT 2 cut(s) 211, 245
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 420
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 80
Bse1I ACTGG 4 cut(s) 89, 117, 401, 410
Bse3DI GCAATG 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 1 cut(s) 420
BseGI GGATG 1 cut(s) 184
BseMI GCAATG 1 cut(s) 366
BseNI ACTGG 4 cut(s) 89, 117, 401, 410
BseXI GCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 1 cut(s) 36
BsiSI CCGG 1 cut(s) 81
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 36
Bsp13I TCCGGA 1 cut(s) 80
Bsp143I GATC 3 cut(s) 211, 245, 318
BspANI GGCC 1 cut(s) 36
BspEI TCCGGA 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 4 cut(s) 89, 117, 401, 410
BssMI GATC 3 cut(s) 211, 245, 318
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 56, 321
BstC8I GCNNGC 2 cut(s) 124, 433
BstF5I GGATG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 3 cut(s) 214, 248, 321
BstMBI GATC 3 cut(s) 211, 245, 318
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 2 cut(s) 127, 276
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 2 cut(s) 211, 245
BstYI RGATCY 2 cut(s) 211, 245
BsuRI GGCC 1 cut(s) 36
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 96, 417
Cac8I GCNNGC 2 cut(s) 124, 433
CfoI GCGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 132, 273
CviAII CATG 1 cut(s) 123
CviJI RGCY 5 cut(s) 36, 73, 88, 140, 386
CviKI_1 RGCY 5 cut(s) 36, 73, 88, 140, 386
CviQI GTAC 2 cut(s) 132, 273
DpnI GATC 3 cut(s) 213, 247, 320
DpnII GATC 3 cut(s) 211, 245, 318
Eam1104I CTCTTC 2 cut(s) 56, 321
EarI CTCTTC 2 cut(s) 56, 321
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 184
FaeI CATG 1 cut(s) 126
FaiI YATR 5 cut(s) 124, 129, 291, 306, 342
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FatI CATG 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 313
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 81
HhaI GCGC 1 cut(s) 170
Hin1II CATG 1 cut(s) 126
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 2 cut(s) 59, 149
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 2 cut(s) 81, 187
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 5 cut(s) 20, 182, 315, 359, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 1 cut(s) 126
HspAI GCGC 1 cut(s) 168
Kpn2I TCCGGA 1 cut(s) 80
Kzo9I GATC 3 cut(s) 211, 245, 318
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 169
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 237
MalI GATC 3 cut(s) 213, 247, 320
MboI GATC 3 cut(s) 211, 245, 318
MboII GAAGA 5 cut(s) 43, 206, 211, 338, 387
MflI RGATCY 2 cut(s) 211, 245
MluCI AATT 5 cut(s) 15, 231, 263, 344, 378
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 2 cut(s) 35, 73
Mph1103I ATGCAT 1 cut(s) 184
MroI TCCGGA 1 cut(s) 80
MseI TTAA 1 cut(s) 219
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 420
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 42
NdeII GATC 3 cut(s) 211, 245, 318
NlaIII CATG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 126
PaeI GCATGC 1 cut(s) 126
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 1 cut(s) 314
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PsuI RGATCY 2 cut(s) 211, 245
RsaI GTAC 2 cut(s) 133, 274
RsaNI GTAC 2 cut(s) 132, 273
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 3 cut(s) 211, 245, 318
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 420
SetI ASST 3 cut(s) 75, 107, 336
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 2 cut(s) 127, 276
SphI GCATGC 1 cut(s) 126
Sse9I AATT 5 cut(s) 15, 231, 263, 344, 378
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 107
TasI AATT 5 cut(s) 15, 231, 263, 344, 378
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 2 cut(s) 96, 417
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 2 cut(s) 206, 212
TspGWI ACGGA 2 cut(s) 272, 382
TspRI CASTG 2 cut(s) 96, 417
XapI RAATTY 4 cut(s) 231, 263, 344, 378
XceI RCATGY 1 cut(s) 126
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.