MD02G1296600.v1.1

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007263
Physical Location & Seq
Reverse (-)
35202002 .. 35205335
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296600.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGAGTAGTATAAATTTTGTTACTCCAAAATCTTCGGTTCAATTTAATGGCGAATCAAGCGGACAAAAAAGTTCTTTGAAAGCGCCCACCAAACTTCAGCCGTTAAGTTCACTTAATAAGCAGGATGAAGTTAAGGCTGTAAAAGCAGTAGATGGTAATTTACCTGTATCGTCAGCAACTCCGGCAGAGAATGGATGTACCGATAATTATGTTAATTGTTCTGCCCATAGTAATGGTGTTAAAGAGGTGACAACTGATAATGTTAACTCAACTTCTTTGCCTTTATCCAAGGACGAAGGTGGTCTCTCCAATCAATTTTCAAGTGCTGATGATTTGTCTGTTTCTAAGATTAAGGGGGAACTACAGAAGGCTTCAAATGCGCCTGCTCTTCTATCCTGCTCTCCTTTTGTTCAGCTTCTGCCGGACTTGAGAACTCTTGAAGTACCTAAGGTTGGCTATCCGACATTGAGTGCATTTGCAGAGTTCGTGTCTGAATTTGACATGCCTAGTGGTTCAAATTCGAAGGACAAAGATCCAAGTGTTCTCGAGACTGGTAGGCCTTTTAGCCCTGCCATGTTTGAAGGCATTCTTAAAAATTTCACTCCAGATGTTCCGCCAAGCATCTCAGGCAGGCCAAGGTTTGTGTCTGATCTTGGTCAATATTTTCTGCCTGGCATATGTTTCTGTACAGTTGGCCTGAAGAATACATCTGCAGTAACCAGGACACAGAGTTTTGTTCCTTCAGAATTGAGTGATATTTTTGGAGGACAATTGAGAAGTGTGGTGAAGGCAACAGGAAATAATGCTTCGGCTACTGTTCAGCCATATTTATTGTTCCACCTTGATATTTACCCTGACGCTGTTGGTACCATTGAAGATGCACTTAAGTTGTATTCTGCACCAGAAACTCTTGAGGGGTATCGAACATCAGCAGCTGGGAAGACCGGTATTGTGACCGCGAGCAAATCCATAAAGATAAAGACACTTTCAAAGATAATGATATTGCATTTGATGCGTTTTGGTTATGGAAGTCAAGGAAGCATAAGCTGCATAAGCCTGTGTATTTTCCACTTGAATTGGTGTTGGGCCGTGAATTGCTTGTTTCTCCAAATACTGAGACTCGTCGCTACAATAACTCATCACGGGAGAGAGCCGTCAAAGGGGCATTATACAACTGATGCTCTCTATTCCAATGGTCAGTGGCTACGCTTTGATGATGCTTCGGTCACGGCAATTGGGACTGGTAAAGTATTGCATAACCAGGCATATGTCCTCATCTACAAACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

431

Amino Acids

46.26

Weight (kDa)

7.99

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 242 - 427 9e-16 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AccII CGCG 1 cut(s) 959
AciI CCGC 3 cut(s) 60, 614, 957
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 4 cut(s) 13, 494, 517, 595
AcuI CTGAAG 3 cut(s) 80, 719, 726
AfaI GTAC 4 cut(s) 199, 444, 688, 868
AfiI CCNNNNNNNGG 2 cut(s) 452, 1160
AflII CTTAAG 1 cut(s) 884
AgeI ACCGGT 1 cut(s) 944
AjnI CCWGG 3 cut(s) 670, 719, 1260
AluBI AGCT 3 cut(s) 415, 935, 1047
AluI AGCT 3 cut(s) 415, 935, 1047
Alw26I GTCTC 3 cut(s) 308, 542, 1111
AlwI GGATC 1 cut(s) 527
AlwNI CAGNNNCTG 2 cut(s) 418, 935
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 4 cut(s) 557, 632, 694, 1086
ApeKI GCWGC 2 cut(s) 932, 1047
ApoI RAATTY 4 cut(s) 13, 494, 517, 595
AsiGI ACCGGT 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 585
Asp718I GGTACC 1 cut(s) 866
AspLEI GCGC 2 cut(s) 85, 382
AspS9I GGNCC 1 cut(s) 1086
AsuHPI GGTGA 2 cut(s) 259, 796
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 545
AxyI CCTNAGG 1 cut(s) 447
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 947
BbvI GCAGC 2 cut(s) 944, 1034
BccI CCATC 1 cut(s) 146
BceAI ACGGC 4 cut(s) 85, 1073, 1138, 1245
BciT130I CCWGG 3 cut(s) 672, 721, 1262
BcoDI GTCTC 3 cut(s) 308, 542, 1111
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 2 cut(s) 362, 711
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 884
BisI GCNGC 2 cut(s) 933, 1048
BlsI GCNGC 2 cut(s) 934, 1049
Bme1390I CCNGG 3 cut(s) 672, 721, 1262
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 1086
BmiI GGNNCC 1 cut(s) 868
BmrFI CCNGG 3 cut(s) 672, 721, 1262
BmsI GCATC 5 cut(s) 630, 868, 1002, 1168, 1207
BpiI GAAGAC 1 cut(s) 947
BpmI CTGGAG 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 448, 932
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 288, 635
BsaWI WCCGGW 1 cut(s) 944
Bsc4I CCNNNNNNNGG 2 cut(s) 452, 1160
Bse118I RCCGGY 1 cut(s) 944
Bse1I ACTGG 2 cut(s) 556, 1246
Bse21I CCTNAGG 1 cut(s) 447
BseBI CCWGG 3 cut(s) 672, 721, 1262
BseDI CCNNGG 2 cut(s) 288, 635
BseGI GGATG 2 cut(s) 130, 200
BseLI CCNNNNNNNGG 2 cut(s) 452, 1160
BseMII CTCAG 2 cut(s) 639, 1106
BseNI ACTGG 2 cut(s) 556, 1246
BseXI GCAGC 2 cut(s) 944, 1034
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 959
BshFI GGCC 4 cut(s) 559, 634, 696, 1088
BshNI GGYRCC 1 cut(s) 866
BshTI ACCGGT 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 3 cut(s) 182, 422, 945
BslFI GGGAC 1 cut(s) 1252
BslI CCNNNNNNNGG 2 cut(s) 452, 1160
BsmAI GTCTC 3 cut(s) 308, 542, 1111
BsmFI GGGAC 1 cut(s) 1252
BsmI GAATGC 1 cut(s) 585
BsnI GGCC 4 cut(s) 559, 634, 696, 1088
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 686
Bsp143I GATC 2 cut(s) 532, 649
BspACI CCGC 3 cut(s) 60, 614, 957
BspANI GGCC 4 cut(s) 559, 634, 696, 1088
BspCNI CTCAG 2 cut(s) 638, 1107
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 1 cut(s) 868
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 884
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 944
BsrGI TGTACA 1 cut(s) 686
BsrI ACTGG 2 cut(s) 556, 1246
BssAI RCCGGY 1 cut(s) 944
BssECI CCNNGG 2 cut(s) 288, 635
BssMI GATC 2 cut(s) 532, 649
BssT1I CCWWGG 2 cut(s) 288, 635
Bst2UI CCWGG 3 cut(s) 672, 721, 1262
Bst4CI ACNGT 2 cut(s) 691, 817
Bst6I CTCTTC 1 cut(s) 393
BstAFI CTTAAG 1 cut(s) 884
BstAPI GCANNNNNTGC 2 cut(s) 1012, 1047
BstAUI TGTACA 1 cut(s) 686
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 3 cut(s) 384, 632, 961
BstDEI CTNAG 4 cut(s) 345, 447, 625, 1115
BstF5I GGATG 2 cut(s) 130, 200
BstFNI CGCG 1 cut(s) 959
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 382
BstKTI GATC 2 cut(s) 535, 652
BstMAI GTCTC 3 cut(s) 308, 542, 1111
BstMBI GATC 2 cut(s) 532, 649
BstMWI GCNNNNNNNGC 8 cut(s) 57, 143, 182, 377, 627, 1012, 1047, 1053
BstNI CCWGG 3 cut(s) 672, 721, 1262
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 3 cut(s) 670, 719, 1260
BstSFI CTRYAG 2 cut(s) 362, 711
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 2 cut(s) 944, 1034
BstV2I GAAGAC 1 cut(s) 947
BstX2I RGATCY 1 cut(s) 532
BstXI CCANNNNNNTGG 1 cut(s) 233
BstYI RGATCY 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 4 cut(s) 559, 634, 696, 1088
BtsCI GGATG 2 cut(s) 130, 200
BtsIMutI CAGTG 1 cut(s) 1205
Cac8I GCNNGC 3 cut(s) 384, 632, 961
CaiI CAGNNNCTG 2 cut(s) 418, 935
CfoI GCGC 2 cut(s) 85, 382
Cfr10I RCCGGY 1 cut(s) 944
Cfr13I GGNCC 1 cut(s) 1086
CseI GACGC 1 cut(s) 866
Csp6I GTAC 4 cut(s) 198, 443, 687, 867
CspAI ACCGGT 1 cut(s) 944
CspCI CAANNNNNGTGG 2 cut(s) 1058, 1093
CviAII CATG 2 cut(s) 502, 574
CviQI GTAC 4 cut(s) 198, 443, 687, 867
DdeI CTNAG 4 cut(s) 345, 447, 625, 1115
DpnI GATC 2 cut(s) 534, 651
DpnII GATC 2 cut(s) 532, 649
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EciI GGCGGA 1 cut(s) 603
Eco130I CCWWGG 2 cut(s) 288, 635
Eco147I AGGCCT 1 cut(s) 559
Eco31I GGTCTC 1 cut(s) 308
Eco57I CTGAAG 3 cut(s) 80, 719, 726
Eco81I CCTNAGG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 670, 719, 1260
EcoT14I CCWWGG 2 cut(s) 288, 635
ErhI CCWWGG 2 cut(s) 288, 635
FaeI CATG 2 cut(s) 505, 577
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 867, 899
FaqI GGGAC 1 cut(s) 1252
FatI CATG 2 cut(s) 501, 573
FauNDI CATATG 2 cut(s) 677, 1267
Fnu4HI GCNGC 2 cut(s) 933, 1048
FokI GGATG 2 cut(s) 137, 207
Fsp4HI GCNGC 2 cut(s) 933, 1048
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 84, 381
GluI GCNGC 2 cut(s) 933, 1048
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 4 cut(s) 559, 634, 696, 1088
HapII CCGG 3 cut(s) 182, 422, 945
HgaI GACGC 1 cut(s) 866
HhaI GCGC 2 cut(s) 85, 382
Hin1II CATG 2 cut(s) 505, 577
Hin6I GCGC 2 cut(s) 83, 380
HinP1I GCGC 2 cut(s) 83, 380
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 53, 1119
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 3 cut(s) 182, 422, 945
HphI GGTGA 2 cut(s) 259, 796
Hpy166II GTNNAC 2 cut(s) 110, 265
Hpy188I TCNGA 4 cut(s) 462, 493, 649, 745
Hpy188III TCNNGA 5 cut(s) 437, 545, 547, 605, 911
Hpy8I GTNNAC 2 cut(s) 110, 265
Hpy99I CGWCG 1 cut(s) 1127
HpyAV CCTTC 6 cut(s) 290, 361, 517, 575, 750, 781
HpyCH4III ACNGT 2 cut(s) 691, 817
HpyCH4V TGCA 8 cut(s) 473, 479, 713, 881, 899, 1006, 1050, 1255
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 143, 182, 377, 627, 1012, 1047, 1053
HpyF3I CTNAG 4 cut(s) 345, 447, 625, 1115
Hsp92II CATG 2 cut(s) 505, 577
HspAI GCGC 2 cut(s) 83, 380
KpnI GGTACC 1 cut(s) 870
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 2 cut(s) 532, 649
LguI GCTCTTC 1 cut(s) 393
Lsp1109I GCAGC 2 cut(s) 944, 1034
LweI GCATC 5 cut(s) 630, 868, 1002, 1168, 1207
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 5 cut(s) 19, 247, 715, 952, 1225
MalI GATC 2 cut(s) 534, 651
MboI GATC 2 cut(s) 532, 649
MboII GAAGA 5 cut(s) 24, 380, 712, 887, 952
MfeI CAATTG 2 cut(s) 770, 1233
MflI RGATCY 1 cut(s) 532
MlyI GAGTC 1 cut(s) 1113
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 4 cut(s) 238, 758, 907, 1283
MroXI GAANNNNTTC 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 935
MspCI CTTAAG 1 cut(s) 884
MspI CCGG 3 cut(s) 182, 422, 945
MspR9I CCNGG 3 cut(s) 672, 721, 1262
MunI CAATTG 2 cut(s) 770, 1233
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 3 cut(s) 672, 721, 1262
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 8 cut(s) 57, 143, 182, 377, 627, 1012, 1047, 1053
NdeI CATATG 2 cut(s) 677, 1267
NdeII GATC 2 cut(s) 532, 649
NlaIII CATG 2 cut(s) 505, 577
NlaIV GGNNCC 1 cut(s) 868
NmuCI GTSAC 3 cut(s) 247, 952, 1225
NspI RCATGY 1 cut(s) 505
NspV TTCGAA 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 393
PctI GAATGC 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 53
PinAI ACCGGT 1 cut(s) 944
PkrI GCNGC 2 cut(s) 934, 1049
PleI GAGTC 1 cut(s) 1113
PpsI GAGTC 1 cut(s) 1113
Psp6I CCWGG 3 cut(s) 670, 719, 1260
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 670, 719, 1260
PspN4I GGNNCC 1 cut(s) 868
PspPI GGNCC 1 cut(s) 1086
PstI CTGCAG 1 cut(s) 715
PstNI CAGNNNCTG 2 cut(s) 418, 935
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 4 cut(s) 199, 444, 688, 868
RsaNI GTAC 4 cut(s) 198, 443, 687, 867
RseI CAYNNNNRTG 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 393
SatI GCNGC 2 cut(s) 933, 1048
Sau3AI GATC 2 cut(s) 532, 649
Sau96I GGNCC 1 cut(s) 1086
SchI GAGTC 1 cut(s) 1113
ScrFI CCNGG 3 cut(s) 672, 721, 1262
SfaNI GCATC 5 cut(s) 630, 868, 1002, 1168, 1207
SfcI CTRYAG 2 cut(s) 362, 711
Sfr274I CTCGAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 521
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 4 cut(s) 427, 545, 884, 911
SmoI CTYRAG 4 cut(s) 427, 545, 884, 911
SseBI AGGCCT 1 cut(s) 559
SsiI CCGC 3 cut(s) 60, 614, 957
SspI AATATT 1 cut(s) 662
SspMI CTAG 1 cut(s) 507
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 3 cut(s) 670, 719, 1260
StyI CCWWGG 2 cut(s) 288, 635
TaaI ACNGT 2 cut(s) 691, 817
TaqI TCGA 3 cut(s) 521, 546, 922
TaqII GACCGA 1 cut(s) 1213
TatI WGTACW 1 cut(s) 686
TfiI GAWTC 1 cut(s) 53
TscAI CASTG 1 cut(s) 1205
TseFI GTSAC 3 cut(s) 247, 952, 1225
TseI GCWGC 2 cut(s) 932, 1047
Tsp45I GTSAC 3 cut(s) 247, 952, 1225
TspDTI ATGAA 1 cut(s) 141
TspRI CASTG 1 cut(s) 1205
Vha464I CTTAAG 1 cut(s) 884
XapI RAATTY 4 cut(s) 13, 494, 517, 595
XceI RCATGY 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.