MD07G1027400.v1.1

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007263
Physical Location & Seq
Forward (+)
2275379 .. 2280374
4996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1027400.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGAGTGATTCTAAGCTGCTAGTATTTGGGTCATTTTCTGAAGACGAGACCAGGTCGTTGCTGCTAAAGCAATCACCCGGGAAGGCGGAAAAGCCTGTGGAAAAGAATGTATTGCAGTTTGGGTCTATAAATTTTGTTTCTCCAAAATCTTCTGGTGAATTTAATGGTGAATCAAGCGGACAGAAAAGTTCTTTGAAAGCGCCCACCAAATCTCAGCCCTTAAGTTCACTTAATAAGCAGAATGAAGTTAAGGCTGTAACAGCAGCAGATAATAATTTACCTGCATCGACAGCCACTCCAACAGAAAATGGATGTACTGATAATTATGTTAATGTTTCTGCCCATAGTAATGGTGTGAAAGAGGTGACAACTGATAATGTTTTCTTAACTTCATTGCCTGTATCCAAAGACGAAGGTGGTCTCTCCAATCAATTTTCAAGTCTGGAATTGCAGAATAGAGAGCAGAATGGCTGTGTTGATGATTTATCTGTTTCTAAGATTAAGGGGGAACTTCAGAAGGCTTCAAATGCGCCTGTTACTGTTTCTAAAATCTTGTTGCCTCGTGGTCTAATCAATTCAGGAAATCTGTGCTTTCTAAATGCAACCCTTCAGGCCCTTCTATCATGCTTTCCCTTTGTTCAGCTTCTTCAGGAATTAAGAACTCTCGAAGTTCCTAAGGTTGACTTTCCAACATTGAGTGCATTTGCAGAGTTCGTCTCTGAATTTGACATGCCTAGTGGTTCAAGTTCGAAGAACAAAGATGCAAGTGTTCTCGAGACTGGTAGGCCTTTTAGCCCTGCCATGTTTGAAGGCGTTCTTAAGAATTTCACTCCAGATGTGCCAACTAACATCTCCGGCAGGCCAAGACAAGAAGATGCTCAGGAGTTCCTTAGCTTCATCATGGATCAGATGCATGATGAATTACTTAAGCGTGAAGGACAATCCCCAAGCATTAATGGGTGCAAATCAACTCTCATATCTTCCGCAGAAGACGATGAATGGGAGACAGTTGGCCCAAAGAATACATCTGCAGTAACCAGGACACAGAGTTTTGTTCCTTCAGAATTGAGTGATATTTTTGGAGGACAATTGAAAAGTGTGGTGAAGGCAAGAGGAAATAAAGCTTCCGCTACTGTTCAGCCATATTTATTGCTCCACCTTGATATTCACCCTGAAGCTGTTCGTACCATTGAAGATGCACTTAAGTTGTTTTCTGCACCAGAAACTCTTGAGGGGTATCGAACATCAGCAGCTGGGAAGGGTGGTATTGTGACTGCAAGCAAATCCATAAAGATACAGACCCTTTCAAAGATAATGATATTGCACTTGATGCGTTTTGGTTATGGAAGCCAAGGAAGCACTAAGCTGCATAAACCCGTGCATTTTCCACTTGAATTGGTGTTGGGTCGTGAATTGCTTGTTTCTCCAAATACTGAGGGTCGAAAATACAAACTTGTTGCTACAATAACTCATCACGGGAGAGAGCCATCAAAGGGACATTATACAACTGATGCTCTCTATGCCAGTCAGTGGCTACGGTTTGACGATGCGTCGGTCACGGCAATTGGGACCGGTAAAGTACTGCATGAGCAGGCATATGTCCTCATCTACAAACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

541

Amino Acids

58.65

Weight (kDa)

6.45

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 188 - 537 2.4e-43 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1621
Acc36I ACCTGC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 1530
AciI CCGC 4 cut(s) 86, 177, 984, 1128
AclWI GGATC 1 cut(s) 912
AcsI RAATTY 4 cut(s) 130, 158, 722, 823
AcuI CTGAAG 6 cut(s) 60, 497, 593, 632, 1044, 1194
AfaI GTAC 3 cut(s) 316, 1186, 1581
AfiI CCNNNNNNNGG 2 cut(s) 1493, 1530
AflII CTTAAG 4 cut(s) 220, 818, 926, 1202
AgeI ACCGGT 1 cut(s) 1571
AgsI TTSAA 9 cut(s) 196, 438, 525, 744, 809, 1093, 1193, 1308, 1394
AhdI GACNNNNNGTC 1 cut(s) 1549
AjnI CCWGG 2 cut(s) 50, 1037
AluBI AGCT 7 cut(s) 16, 643, 894, 1124, 1178, 1253, 1366
AluI AGCT 7 cut(s) 16, 643, 894, 1124, 1178, 1253, 1366
Alw26I GTCTC 5 cut(s) 41, 425, 721, 770, 998
AlwI GGATC 1 cut(s) 912
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 2 cut(s) 77, 773
AoxI GGCC 4 cut(s) 612, 785, 860, 1012
ApeKI GCWGC 5 cut(s) 16, 61, 263, 1250, 1366
ApoI RAATTY 4 cut(s) 130, 158, 722, 823
AseI ATTAAT 1 cut(s) 954
AsiGI ACCGGT 1 cut(s) 1571
Asp700I GAANNNNTTC 2 cut(s) 813, 1179
AspLEI GCGC 2 cut(s) 202, 532
AspS9I GGNCC 3 cut(s) 613, 1013, 1569
AsuC2I CCSGG 2 cut(s) 78, 79
AsuHPI GGTGA 6 cut(s) 66, 167, 179, 376, 1114, 1160
AsuII TTCGAA 1 cut(s) 749
AvaI CYCGRG 2 cut(s) 77, 773
AvaII GGWCC 1 cut(s) 1569
AxyI CCTNAGG 1 cut(s) 675
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 48, 996
BbvI GCAGC 5 cut(s) 3, 48, 275, 1262, 1353
BccI CCATC 1 cut(s) 1495
BceAI ACGGC 1 cut(s) 1575
BciT130I CCWGG 2 cut(s) 52, 1039
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 2 cut(s) 78, 79
BcoDI GTCTC 5 cut(s) 41, 425, 721, 770, 998
BfaI CTAG 2 cut(s) 20, 735
BfmI CTRYAG 1 cut(s) 1029
BfoI RGCGCY 1 cut(s) 203
BfrI CTTAAG 4 cut(s) 220, 818, 926, 1202
BfuAI ACCTGC 1 cut(s) 289
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 5 cut(s) 17, 62, 264, 1251, 1367
BlsI GCNGC 5 cut(s) 18, 63, 265, 1252, 1368
BmcAI AGTACT 1 cut(s) 1581
Bme1390I CCNGG 4 cut(s) 52, 78, 79, 1039
Bme18I GGWCC 1 cut(s) 1569
BmeRI GACNNNNNGTC 1 cut(s) 1549
BmeT110I CYCGRG 2 cut(s) 77, 773
BmgT120I GGNCC 3 cut(s) 613, 1013, 1569
BmiI GGNNCC 1 cut(s) 1570
BmrFI CCNGG 4 cut(s) 52, 78, 79, 1039
BmsI GCATC 8 cut(s) 293, 751, 865, 900, 1186, 1320, 1501, 1537
BpiI GAAGAC 2 cut(s) 48, 996
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 2 cut(s) 879, 890
Bpu14I TTCGAA 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 1250
BpuMI CCSGG 2 cut(s) 78, 79
BsaI GGTCTC 2 cut(s) 41, 425
BsaJI CCNNGG 2 cut(s) 77, 1351
BsaWI WCCGGW 1 cut(s) 1571
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 1493, 1530
Bse118I RCCGGY 1 cut(s) 1571
Bse1I ACTGG 2 cut(s) 784, 1524
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 392
BseBI CCWGG 2 cut(s) 52, 1039
BseDI CCNNGG 2 cut(s) 77, 1351
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 1493, 1530
BseMI GCAATG 1 cut(s) 392
BseMII CTCAG 3 cut(s) 227, 893, 1425
BseNI ACTGG 2 cut(s) 784, 1524
BseXI GCAGC 5 cut(s) 3, 48, 275, 1262, 1353
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 1200
BshFI GGCC 4 cut(s) 614, 787, 862, 1014
BshTI ACCGGT 1 cut(s) 1571
BsiHKCI CYCGRG 2 cut(s) 77, 773
BsiSI CCGG 3 cut(s) 78, 855, 1572
BslFI GGGAC 2 cut(s) 1509, 1582
BslI CCNNNNNNNGG 2 cut(s) 1493, 1530
BsmAI GTCTC 5 cut(s) 41, 425, 721, 770, 998
BsmBI CGTCTC 1 cut(s) 721
BsmFI GGGAC 2 cut(s) 1509, 1582
BsnI GGCC 4 cut(s) 614, 787, 862, 1014
Bso31I GGTCTC 2 cut(s) 41, 425
BsoBI CYCGRG 2 cut(s) 77, 773
Bsp119I TTCGAA 1 cut(s) 749
Bsp143I GATC 1 cut(s) 904
BspACI CCGC 4 cut(s) 86, 177, 984, 1128
BspANI GGCC 4 cut(s) 614, 787, 862, 1014
BspCNI CTCAG 3 cut(s) 226, 892, 1426
BspLI GGNNCC 1 cut(s) 1570
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 912
BspT104I TTCGAA 1 cut(s) 749
BspTI CTTAAG 4 cut(s) 220, 818, 926, 1202
BspTNI GGTCTC 2 cut(s) 41, 425
BsrDI GCAATG 1 cut(s) 392
BsrFI RCCGGY 1 cut(s) 1571
BsrI ACTGG 2 cut(s) 784, 1524
BssAI RCCGGY 1 cut(s) 1571
BssECI CCNNGG 2 cut(s) 77, 1351
BssMI GATC 1 cut(s) 904
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 1351
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 52, 1039
Bst4CI ACNGT 4 cut(s) 541, 1009, 1135, 1539
BstAFI CTTAAG 4 cut(s) 220, 818, 926, 1202
BstAPI GCANNNNNTGC 1 cut(s) 1330
BstBI TTCGAA 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 860, 1279, 1593
BstDEI CTNAG 8 cut(s) 12, 213, 495, 675, 879, 890, 1362, 1434
BstF5I GGATG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 202, 532
BstKTI GATC 1 cut(s) 907
BstMAI GTCTC 5 cut(s) 41, 425, 721, 770, 998
BstMBI GATC 1 cut(s) 904
BstMWI GCNNNNNNNGC 7 cut(s) 67, 260, 290, 527, 1330, 1356, 1520
BstNI CCWGG 2 cut(s) 52, 1039
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 4 cut(s) 50, 76, 77, 1037
BstSFI CTRYAG 1 cut(s) 1029
BstV1I GCAGC 5 cut(s) 3, 48, 275, 1262, 1353
BstV2I GAAGAC 2 cut(s) 48, 996
BstXI CCANNNNNNTGG 1 cut(s) 350
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 4 cut(s) 614, 787, 862, 1014
BtsCI GGATG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 1535
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 860, 1279, 1593
CaiI CAGNNNCTG 1 cut(s) 1253
CfoI GCGC 2 cut(s) 202, 532
Cfr10I RCCGGY 1 cut(s) 1571
Cfr13I GGNCC 3 cut(s) 613, 1013, 1569
Cfr9I CCCGGG 1 cut(s) 77
CseI GACGC 1 cut(s) 1539
CsiI ACCWGGT 1 cut(s) 50
Csp6I GTAC 3 cut(s) 315, 1185, 1580
CspAI ACCGGT 1 cut(s) 1571
CspCI CAANNNNNGTGG 2 cut(s) 1377, 1412
CviAII CATG 6 cut(s) 624, 730, 802, 901, 914, 1586
CviQI GTAC 3 cut(s) 315, 1185, 1580
DdeI CTNAG 8 cut(s) 12, 213, 495, 675, 879, 890, 1362, 1434
DpnI GATC 1 cut(s) 906
DpnII GATC 1 cut(s) 904
DriI GACNNNNNGTC 1 cut(s) 1549
Eam1105I GACNNNNNGTC 1 cut(s) 1549
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 1351
Eco147I AGGCCT 1 cut(s) 787
Eco31I GGTCTC 2 cut(s) 41, 425
Eco47I GGWCC 1 cut(s) 1569
Eco57I CTGAAG 6 cut(s) 60, 497, 593, 632, 1044, 1194
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 2 cut(s) 77, 773
EcoO109I RGGNCCY 1 cut(s) 613
EcoRII CCWGG 2 cut(s) 50, 1037
EcoT14I CCWWGG 1 cut(s) 1351
EcoT22I ATGCAT 1 cut(s) 915
ErhI CCWWGG 1 cut(s) 1351
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 6 cut(s) 627, 733, 805, 904, 917, 1589
FalI AAGNNNNNCTT 6 cut(s) 668, 700, 801, 833, 1185, 1217
FaqI GGGAC 2 cut(s) 1509, 1582
FatI CATG 6 cut(s) 623, 729, 801, 900, 913, 1585
FauNDI CATATG 1 cut(s) 1597
Fnu4HI GCNGC 5 cut(s) 17, 62, 264, 1251, 1367
FokI GGATG 1 cut(s) 324
Fsp4HI GCNGC 5 cut(s) 17, 62, 264, 1251, 1367
FspBI CTAG 2 cut(s) 20, 735
GlaI GCGC 2 cut(s) 201, 531
GluI GCNGC 5 cut(s) 17, 62, 264, 1251, 1367
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 4 cut(s) 614, 787, 862, 1014
HapII CCGG 3 cut(s) 78, 855, 1572
HgaI GACGC 1 cut(s) 1539
HhaI GCGC 2 cut(s) 202, 532
Hin1II CATG 6 cut(s) 627, 733, 805, 904, 917, 1589
Hin6I GCGC 2 cut(s) 200, 530
HinP1I GCGC 2 cut(s) 200, 530
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 1 cut(s) 1122
HinfI GANTC 2 cut(s) 8, 170
HpaII CCGG 3 cut(s) 78, 855, 1572
HphI GGTGA 6 cut(s) 66, 167, 179, 376, 1114, 1160
Hpy166II GTNNAC 2 cut(s) 227, 682
Hpy188I TCNGA 5 cut(s) 40, 516, 721, 909, 1063
Hpy8I GTNNAC 2 cut(s) 227, 682
Hpy99I CGWCG 1 cut(s) 1555
HpyCH4III ACNGT 4 cut(s) 541, 1009, 1135, 1539
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 260, 290, 527, 1330, 1356, 1520
HpyF3I CTNAG 8 cut(s) 12, 213, 495, 675, 879, 890, 1362, 1434
Hsp92II CATG 6 cut(s) 627, 733, 805, 904, 917, 1589
HspAI GCGC 2 cut(s) 200, 530
Kzo9I GATC 1 cut(s) 904
LmnI GCTCC 1 cut(s) 1158
Lsp1109I GCAGC 5 cut(s) 3, 48, 275, 1262, 1353
LweI GCATC 8 cut(s) 293, 751, 865, 900, 1186, 1320, 1501, 1537
MabI ACCWGGT 1 cut(s) 50
MaeI CTAG 2 cut(s) 20, 735
MaeIII GTNAC 6 cut(s) 256, 364, 535, 1033, 1270, 1555
MalI GATC 1 cut(s) 906
MboI GATC 1 cut(s) 904
MboII GAAGA 8 cut(s) 53, 141, 638, 763, 884, 972, 1001, 1205
MfeI CAATTG 2 cut(s) 1088, 1563
MmeI TCCRAC 2 cut(s) 323, 713
MnlI CCTC 7 cut(s) 355, 570, 1076, 1106, 1225, 1429, 1613
Mph1103I ATGCAT 1 cut(s) 915
MroXI GAANNNNTTC 2 cut(s) 813, 1179
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 1253
MspCI CTTAAG 4 cut(s) 220, 818, 926, 1202
MspI CCGG 3 cut(s) 78, 855, 1572
MspR9I CCNGG 4 cut(s) 52, 78, 79, 1039
MunI CAATTG 2 cut(s) 1088, 1563
MvaI CCWGG 2 cut(s) 52, 1039
MwoI GCNNNNNNNGC 7 cut(s) 67, 260, 290, 527, 1330, 1356, 1520
NciI CCSGG 2 cut(s) 78, 79
NdeI CATATG 1 cut(s) 1597
NdeII GATC 1 cut(s) 904
NlaIII CATG 6 cut(s) 627, 733, 805, 904, 917, 1589
NlaIV GGNNCC 1 cut(s) 1570
NmuCI GTSAC 3 cut(s) 364, 1270, 1555
NsiI ATGCAT 1 cut(s) 915
NspI RCATGY 1 cut(s) 733
NspV TTCGAA 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 773
PceI AGGCCT 1 cut(s) 787
PdmI GAANNNNTTC 2 cut(s) 813, 1179
PfeI GAWTC 2 cut(s) 8, 170
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 1530
PinAI ACCGGT 1 cut(s) 1571
PkrI GCNGC 5 cut(s) 18, 63, 265, 1252, 1368
PshBI ATTAAT 1 cut(s) 954
PsiI TTATAA 1 cut(s) 1621
Psp6I CCWGG 2 cut(s) 50, 1037
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 2 cut(s) 50, 1037
PspN4I GGNNCC 1 cut(s) 1570
PspPI GGNCC 3 cut(s) 613, 1013, 1569
PstI CTGCAG 1 cut(s) 1033
PstNI CAGNNNCTG 1 cut(s) 1253
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1253
RsaI GTAC 3 cut(s) 316, 1186, 1581
RsaNI GTAC 3 cut(s) 315, 1185, 1580
RseI CAYNNNNRTG 1 cut(s) 348
SatI GCNGC 5 cut(s) 17, 62, 264, 1251, 1367
Sau3AI GATC 1 cut(s) 904
Sau96I GGNCC 3 cut(s) 613, 1013, 1569
ScaI AGTACT 1 cut(s) 1581
ScrFI CCNGG 4 cut(s) 52, 78, 79, 1039
SexAI ACCWGGT 1 cut(s) 50
SfaNI GCATC 8 cut(s) 293, 751, 865, 900, 1186, 1320, 1501, 1537
SfcI CTRYAG 1 cut(s) 1029
Sfr274I CTCGAG 1 cut(s) 773
SfuI TTCGAA 1 cut(s) 749
SinI GGWCC 1 cut(s) 1569
SlaI CTCGAG 1 cut(s) 773
SmaI CCCGGG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 6 cut(s) 220, 773, 818, 926, 1202, 1229
SmoI CTYRAG 6 cut(s) 220, 773, 818, 926, 1202, 1229
SseBI AGGCCT 1 cut(s) 787
SsiI CCGC 4 cut(s) 86, 177, 984, 1128
SspMI CTAG 2 cut(s) 20, 735
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 4 cut(s) 50, 76, 77, 1037
StyI CCWWGG 1 cut(s) 1351
TaaI ACNGT 4 cut(s) 541, 1009, 1135, 1539
TaqI TCGA 6 cut(s) 287, 666, 749, 774, 1240, 1441
TaqII GACCGA 1 cut(s) 1543
TatI WGTACW 2 cut(s) 314, 1579
TfiI GAWTC 2 cut(s) 8, 170
TscAI CASTG 1 cut(s) 1535
TseFI GTSAC 3 cut(s) 364, 1270, 1555
TseI GCWGC 5 cut(s) 16, 61, 263, 1250, 1366
Tsp45I GTSAC 3 cut(s) 364, 1270, 1555
TspDTI ATGAA 5 cut(s) 258, 381, 886, 933, 1011
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 1 cut(s) 1535
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 1530
Vha464I CTTAAG 4 cut(s) 220, 818, 926, 1202
VpaK11BI GGWCC 1 cut(s) 1569
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 4 cut(s) 130, 158, 722, 823
XceI RCATGY 1 cut(s) 733
XhoI CTCGAG 1 cut(s) 773
XmaI CCCGGG 1 cut(s) 77
XmnI GAANNNNTTC 2 cut(s) 813, 1179
XspI CTAG 2 cut(s) 20, 735
ZrmI AGTACT 1 cut(s) 1581
Zsp2I ATGCAT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.