MD02G1296700.v1.1

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007263
Physical Location & Seq
Reverse (-)
35217218 .. 35221604
4387 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296700.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGAGTGATTCTAAGTTACTAGTGTTTGGGTCATTCTCTGAAGACGAGACCAGGTCGTTGCTGGTAAAGCAATCACCTGTCAAGGCTGAGAAGCCTGTGGAGAAGAATGTATTGCAGTTTGGGTCTATAAATTTTGTTTCTGCAAAATCTTCGGTTCAATTTAATGGCGAATCAAGCGGACAGAAAAGTTCTTTGAAGGCGCCCACCAAACTTCAGCCGTTAAGTTCACTTAAGCAGGATGAAGTTAAGGCTGTAAAAGCAGTAGATGGTAATTTACCTGCATCGTCAGCAACTCCGGCAGAGAATGGATGTACCGATAATTATGTTAATTGTTCTGCCCATAGTAATGGTGTTAAAGAGGTGACAACTGATAATGTTAACTTAACTTCCTTGCCTTTATCCAAGGAGGAAGGTGGTCTCTCCAATCAATTTTCAAGTCTGGAATTGCAGAATAGAGAGCAGAATGGCAGTGTTGATGACTTGTCTGTTTCTAAGATTAAGGGGGAACTACAGAAGAGTTCAAATGCGCCTGTTACAGTTTCTAAAAGCTTATTGCCTCGTGGTCTAATCAATTCAGGGAATCTGTGCTTTCTAAATGCAACCCTGCAGGCTCTTCTATCCTGCTCTCCTTTTGTTCAGCTTCTGCAGGAATTGAGAACTCTTGAAGTACCTAAGGTTGGCTATCCGACATTGAGTGCATTTGCAGAGTTCGTGTCTGAATTTGACATGCCTAGTGGTTCAAGTTCGAAGGACAGAGATGTAAGTGTTCTCGAGACTGGTAGGCCTTTTAGCCCTGCCATGTTTGAAGGCGTTCTTAAAAATTTCACTCCAGATGTTCTGCCTAACATCTCCGGCAGGCCAAGACAAGAGGATGCTCAGGAGTTCCTTAGCTTTATCATGGATCAGATGCATGATGAATTACTTAAGCTTGAAGGACAATCCCCCAGCATTAACGGGCGCAAATCATCTCTTATATCTTCACCAGAAGATGATGAATGGGAAACAGTTGGCCCGAAGAATACATCTGCCGTAACCAGGACACAGAGTTTTGTTCCTTCAGAATTGAGTGATATTTTTGGAGGACAATTGAGAAGTGTGGTGAAGGCAAGAGGAAATAAAGCTTCTGCTACTGTTCAGCCATATTTATTGCTCCACCTTGATATTTACCCTGATGCTGTTCGTACCATTGAAGATGCACTTAAGTTGTATTCTGCACCAGAAACTCTTGAGGGGTATCGAACATCAGCAGCTGGGAAGGCCGGTATTGTGACTGCGAGCAAATCCATAAAGATAAAGACACTTTCAAAGATAATGATATTGCACTTGATGCGTTTTGGTTATGGAAGTCAAGGAAGCACTAAGCTGCATAAGCCTGTGAATTTTCCACTTGAATTGGTGTTGGGCCGTGAATTGCTTCTTTCTCCAAATACTGAGGGTCGAAAATACAAACTCGTTGCTACAATAACTCATCACGGGAGAGAGCCGTCAAAGGGGCATTATACAACTGATGCTCTCTATGCCAATGGTCAGTGGCTACGCTTTGATGATGCTTCGGTCACGGCAATTGGGACTGGTAAAGTATTGCATAACCAGGCATATGTCCTCATCTACAAACAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

541

Amino Acids

58.64

Weight (kDa)

7.13

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 187 - 537 3.8e-45 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AccB1I GGYRCC 1 cut(s) 199
AciI CCGC 1 cut(s) 177
AclWI GGATC 1 cut(s) 909
AcsI RAATTY 4 cut(s) 130, 719, 820, 1378
AcuI CTGAAG 3 cut(s) 60, 197, 1041
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 3 cut(s) 313, 669, 1183
AfiI CCNNNNNNNGG 3 cut(s) 677, 1035, 1490
AflII CTTAAG 3 cut(s) 230, 923, 1199
AhlI ACTAGT 1 cut(s) 19
AjnI CCWGG 3 cut(s) 50, 1034, 1590
AluBI AGCT 7 cut(s) 549, 640, 891, 928, 1121, 1250, 1363
AluI AGCT 7 cut(s) 549, 640, 891, 928, 1121, 1250, 1363
Alw26I GTCTC 3 cut(s) 41, 422, 767
AlwI GGATC 1 cut(s) 909
AlwNI CAGNNNCTG 2 cut(s) 643, 1250
Ama87I CYCGRG 1 cut(s) 770
AoxI GGCC 5 cut(s) 782, 857, 1009, 1257, 1402
ApeKI GCWGC 2 cut(s) 1247, 1363
ApoI RAATTY 4 cut(s) 130, 719, 820, 1378
Asp700I GAANNNNTTC 2 cut(s) 810, 1413
AspLEI GCGC 3 cut(s) 202, 529, 960
AspS9I GGNCC 2 cut(s) 1010, 1402
AsuHPI GGTGA 4 cut(s) 66, 373, 972, 1111
AsuII TTCGAA 1 cut(s) 746
AvaI CYCGRG 1 cut(s) 770
AxyI CCTNAGG 1 cut(s) 672
BanI GGYRCC 1 cut(s) 199
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 1259, 1350
BccI CCATC 1 cut(s) 260
BceAI ACGGC 5 cut(s) 202, 1013, 1389, 1468, 1575
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BciT130I CCWGG 3 cut(s) 52, 1036, 1592
BcoDI GTCTC 3 cut(s) 41, 422, 767
BcuI ACTAGT 1 cut(s) 19
BfaI CTAG 2 cut(s) 20, 732
BfmI CTRYAG 3 cut(s) 509, 605, 644
BfoI RGCGCY 1 cut(s) 203
BfrI CTTAAG 3 cut(s) 230, 923, 1199
BfuAI ACCTGC 1 cut(s) 286
BisI GCNGC 2 cut(s) 1248, 1364
BlsI GCNGC 2 cut(s) 1249, 1365
Bme1390I CCNGG 3 cut(s) 52, 1036, 1592
BmeT110I CYCGRG 1 cut(s) 770
BmgT120I GGNCC 2 cut(s) 1010, 1402
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 3 cut(s) 52, 1036, 1592
BmsI GCATC 8 cut(s) 290, 862, 897, 1162, 1183, 1317, 1498, 1537
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 2 cut(s) 876, 887
Bpu14I TTCGAA 1 cut(s) 746
BpuEI CTTGAG 1 cut(s) 1247
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 2 cut(s) 41, 422
BsaJI CCNNGG 1 cut(s) 402
Bsc4I CCNNNNNNNGG 3 cut(s) 677, 1035, 1490
Bse118I RCCGGY 1 cut(s) 1259
Bse1I ACTGG 2 cut(s) 781, 1576
Bse21I CCTNAGG 1 cut(s) 672
BseBI CCWGG 3 cut(s) 52, 1036, 1592
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 3 cut(s) 244, 314, 877
BseLI CCNNNNNNNGG 3 cut(s) 677, 1035, 1490
BseMII CTCAG 3 cut(s) 78, 890, 1422
BseNI ACTGG 2 cut(s) 781, 1576
BseXI GCAGC 2 cut(s) 1259, 1350
BseYI CCCAGC 2 cut(s) 944, 1250
BsgI GTGCAG 1 cut(s) 1197
BshFI GGCC 5 cut(s) 784, 859, 1011, 1259, 1404
BshNI GGYRCC 1 cut(s) 199
BsiHKCI CYCGRG 1 cut(s) 770
BsiSI CCGG 3 cut(s) 296, 852, 1260
BslFI GGGAC 1 cut(s) 1582
BslI CCNNNNNNNGG 3 cut(s) 677, 1035, 1490
BsmAI GTCTC 3 cut(s) 41, 422, 767
BsmFI GGGAC 1 cut(s) 1582
BsnI GGCC 5 cut(s) 784, 859, 1011, 1259, 1404
Bso31I GGTCTC 2 cut(s) 41, 422
BsoBI CYCGRG 1 cut(s) 770
Bsp119I TTCGAA 1 cut(s) 746
Bsp143I GATC 1 cut(s) 901
BspACI CCGC 1 cut(s) 177
BspANI GGCC 5 cut(s) 784, 859, 1011, 1259, 1404
BspCNI CTCAG 3 cut(s) 79, 889, 1423
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 2 cut(s) 609, 648
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 909
BspQI GCTCTTC 1 cut(s) 618
BspT104I TTCGAA 1 cut(s) 746
BspT107I GGYRCC 1 cut(s) 199
BspTI CTTAAG 3 cut(s) 230, 923, 1199
BspTNI GGTCTC 2 cut(s) 41, 422
BsrFI RCCGGY 1 cut(s) 1259
BsrI ACTGG 2 cut(s) 781, 1576
BssAI RCCGGY 1 cut(s) 1259
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 1 cut(s) 901
BssNI GRCGYC 1 cut(s) 200
BssSI CACGAG 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 3 cut(s) 52, 1036, 1592
Bst4CI ACNGT 3 cut(s) 538, 1006, 1132
Bst6I CTCTTC 2 cut(s) 509, 618
BstACI GRCGYC 1 cut(s) 200
BstAFI CTTAAG 3 cut(s) 230, 923, 1199
BstAPI GCANNNNNTGC 1 cut(s) 1327
BstBI TTCGAA 1 cut(s) 746
BstC8I GCNNGC 3 cut(s) 609, 857, 1276
BstDEI CTNAG 8 cut(s) 12, 87, 492, 672, 876, 887, 1359, 1431
BstF5I GGATG 3 cut(s) 244, 314, 877
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 3 cut(s) 202, 529, 960
BstKTI GATC 1 cut(s) 904
BstMAI GTCTC 3 cut(s) 41, 422, 767
BstMBI GATC 1 cut(s) 901
BstMWI GCNNNNNNNGC 9 cut(s) 67, 174, 257, 287, 296, 1256, 1327, 1369, 1517
BstNI CCWGG 3 cut(s) 52, 1036, 1592
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 3 cut(s) 50, 1034, 1590
BstSFI CTRYAG 3 cut(s) 509, 605, 644
BstV1I GCAGC 2 cut(s) 1259, 1350
BstV2I GAAGAC 1 cut(s) 48
BstXI CCANNNNNNTGG 1 cut(s) 347
Bsu36I CCTNAGG 1 cut(s) 672
BsuRI GGCC 5 cut(s) 784, 859, 1011, 1259, 1404
BtsCI GGATG 3 cut(s) 244, 314, 877
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 2 cut(s) 475, 1535
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 609, 857, 1276
CaiI CAGNNNCTG 2 cut(s) 643, 1250
CfoI GCGC 3 cut(s) 202, 529, 960
Cfr10I RCCGGY 1 cut(s) 1259
Cfr13I GGNCC 2 cut(s) 1010, 1402
CsiI ACCWGGT 1 cut(s) 50
Csp6I GTAC 3 cut(s) 312, 668, 1182
CspCI CAANNNNNGTGG 2 cut(s) 1374, 1409
CviAII CATG 4 cut(s) 727, 799, 898, 911
CviQI GTAC 3 cut(s) 312, 668, 1182
DdeI CTNAG 8 cut(s) 12, 87, 492, 672, 876, 887, 1359, 1431
DinI GGCGCC 1 cut(s) 201
DpnI GATC 1 cut(s) 903
DpnII GATC 1 cut(s) 901
Eam1104I CTCTTC 2 cut(s) 509, 618
EarI CTCTTC 2 cut(s) 509, 618
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 784
Eco31I GGTCTC 2 cut(s) 41, 422
Eco57I CTGAAG 3 cut(s) 60, 197, 1041
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 770
EcoRII CCWGG 3 cut(s) 50, 1034, 1590
EcoT14I CCWWGG 1 cut(s) 402
EcoT22I ATGCAT 1 cut(s) 912
EgeI GGCGCC 1 cut(s) 201
EheI GGCGCC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 730, 802, 901, 914
FalI AAGNNNNNCTT 4 cut(s) 798, 830, 1182, 1214
FaqI GGGAC 1 cut(s) 1582
FatI CATG 4 cut(s) 726, 798, 897, 910
FauNDI CATATG 1 cut(s) 1597
Fnu4HI GCNGC 2 cut(s) 1248, 1364
FokI GGATG 3 cut(s) 251, 321, 884
Fsp4HI GCNGC 2 cut(s) 1248, 1364
FspBI CTAG 2 cut(s) 20, 732
GlaI GCGC 3 cut(s) 201, 528, 959
GluI GCNGC 2 cut(s) 1248, 1364
GsaI CCCAGC 2 cut(s) 948, 1254
GsuI CTGGAG 1 cut(s) 813
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 5 cut(s) 784, 859, 1011, 1259, 1404
HapII CCGG 3 cut(s) 296, 852, 1260
HhaI GCGC 3 cut(s) 202, 529, 960
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 4 cut(s) 730, 802, 901, 914
Hin6I GCGC 3 cut(s) 200, 527, 958
HinP1I GCGC 3 cut(s) 200, 527, 958
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 3 cut(s) 547, 926, 1119
HinfI GANTC 3 cut(s) 8, 170, 580
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 3 cut(s) 296, 852, 1260
HphI GGTGA 4 cut(s) 66, 373, 972, 1111
Hpy166II GTNNAC 2 cut(s) 227, 379
Hpy188I TCNGA 5 cut(s) 40, 687, 718, 906, 1060
Hpy188III TCNNGA 7 cut(s) 440, 662, 770, 772, 830, 878, 1226
Hpy8I GTNNAC 2 cut(s) 227, 379
HpyAV CCTTC 8 cut(s) 190, 404, 742, 800, 926, 1065, 1096, 1249
HpyCH4III ACNGT 3 cut(s) 538, 1006, 1132
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 174, 257, 287, 296, 1256, 1327, 1369, 1517
HpyF3I CTNAG 8 cut(s) 12, 87, 492, 672, 876, 887, 1359, 1431
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 4 cut(s) 730, 802, 901, 914
HspAI GCGC 3 cut(s) 200, 527, 958
KasI GGCGCC 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 901
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 1 cut(s) 1155
Lsp1109I GCAGC 2 cut(s) 1259, 1350
LweI GCATC 8 cut(s) 290, 862, 897, 1162, 1183, 1317, 1498, 1537
MabI ACCWGGT 1 cut(s) 50
MaeI CTAG 2 cut(s) 20, 732
MaeIII GTNAC 6 cut(s) 15, 361, 532, 1030, 1267, 1555
MalI GATC 1 cut(s) 903
MboI GATC 1 cut(s) 901
MboII GAAGA 9 cut(s) 53, 115, 141, 526, 605, 969, 998, 1027, 1202
MfeI CAATTG 2 cut(s) 1085, 1563
Mly113I GGCGCC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 710
MnlI CCTC 9 cut(s) 352, 400, 567, 862, 1073, 1103, 1222, 1426, 1613
Mph1103I ATGCAT 1 cut(s) 912
MroXI GAANNNNTTC 2 cut(s) 810, 1413
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 1250
MspCI CTTAAG 3 cut(s) 230, 923, 1199
MspI CCGG 3 cut(s) 296, 852, 1260
MspR9I CCNGG 3 cut(s) 52, 1036, 1592
MunI CAATTG 2 cut(s) 1085, 1563
MvaI CCWGG 3 cut(s) 52, 1036, 1592
MwoI GCNNNNNNNGC 9 cut(s) 67, 174, 257, 287, 296, 1256, 1327, 1369, 1517
NarI GGCGCC 1 cut(s) 200
NdeI CATATG 1 cut(s) 1597
NdeII GATC 1 cut(s) 901
NlaIII CATG 4 cut(s) 730, 802, 901, 914
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 3 cut(s) 361, 1267, 1555
NsiI ATGCAT 1 cut(s) 912
NspI RCATGY 1 cut(s) 730
NspV TTCGAA 1 cut(s) 746
PaeR7I CTCGAG 1 cut(s) 770
PceI AGGCCT 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 618
PdmI GAANNNNTTC 2 cut(s) 810, 1413
PfeI GAWTC 3 cut(s) 8, 170, 580
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 1249, 1365
PluTI GGCGCC 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 50, 1034, 1590
PspFI CCCAGC 2 cut(s) 944, 1250
PspGI CCWGG 3 cut(s) 50, 1034, 1590
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 2 cut(s) 1010, 1402
PstI CTGCAG 2 cut(s) 609, 648
PstNI CAGNNNCTG 2 cut(s) 643, 1250
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 3 cut(s) 313, 669, 1183
RsaNI GTAC 3 cut(s) 312, 668, 1182
RseI CAYNNNNRTG 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 618
SatI GCNGC 2 cut(s) 1248, 1364
Sau3AI GATC 1 cut(s) 901
Sau96I GGNCC 2 cut(s) 1010, 1402
SbfI CCTGCAGG 1 cut(s) 609
ScrFI CCNGG 3 cut(s) 52, 1036, 1592
SdaI CCTGCAGG 1 cut(s) 609
SexAI ACCWGGT 1 cut(s) 50
SfaNI GCATC 8 cut(s) 290, 862, 897, 1162, 1183, 1317, 1498, 1537
SfcI CTRYAG 3 cut(s) 509, 605, 644
SfoI GGCGCC 1 cut(s) 201
Sfr274I CTCGAG 1 cut(s) 770
SfuI TTCGAA 1 cut(s) 746
SlaI CTCGAG 1 cut(s) 770
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 5 cut(s) 230, 770, 923, 1199, 1226
SmoI CTYRAG 5 cut(s) 230, 770, 923, 1199, 1226
SpeI ACTAGT 1 cut(s) 19
Sse8387I CCTGCAGG 1 cut(s) 609
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 1 cut(s) 177
SspDI GGCGCC 1 cut(s) 199
SspMI CTAG 2 cut(s) 20, 732
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 3 cut(s) 50, 1034, 1590
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 3 cut(s) 538, 1006, 1132
TaqI TCGA 4 cut(s) 746, 771, 1237, 1438
TaqII GACCGA 1 cut(s) 1543
TfiI GAWTC 3 cut(s) 8, 170, 580
TscAI CASTG 2 cut(s) 475, 1535
TseFI GTSAC 3 cut(s) 361, 1267, 1555
TseI GCWGC 2 cut(s) 1247, 1363
Tsp45I GTSAC 3 cut(s) 361, 1267, 1555
TspDTI ATGAA 3 cut(s) 255, 930, 1008
TspRI CASTG 2 cut(s) 475, 1535
Tth111I GACNNNGTC 1 cut(s) 52
Vha464I CTTAAG 3 cut(s) 230, 923, 1199
XapI RAATTY 4 cut(s) 130, 719, 820, 1378
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XhoI CTCGAG 1 cut(s) 770
XmnI GAANNNNTTC 2 cut(s) 810, 1413
XspI CTAG 2 cut(s) 20, 732
Zsp2I ATGCAT 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.