Rh1CG039100

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007263
Physical Location & Seq
Forward (+)
7498713 .. 7505031
6319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG039100.1

Sequence Viewer

Length: 1647 bp
ATGCCGTTATCGTTGTGGAATTTTACTTTTGTGTTTATTCCTTGGTTGCAGCTATTCATATTTGGGTCATTCTCTGAAGATGAGACCAAGTCATTGCTTCTAAAGCAATCACCGGGGAAGCCGTCACCTGTGAAGGGTGAAAAGCCAGTGGAAACAAACGTATTGCAGTTTGGTTCGATAAATTTTGTTACGGATAAATCTTTAGTTGAGTTGAATGGGGGATCAAGTAGACAGCAAAGTTGTTTGAAGGGGCCGAGCAAATTGCAGTCTCTAAATGCAGATAATAAGCACAATGAAGTTAAGACTGCAAAAGCTGGAGATAATAATTTACTAGGATCAGTGGCAATTCCGACACAGAATGGGTGTGCTGATCATCACAGTCACAGTTCTACTCATAGTAATGGTGTAAAAGAGGTTACGACAGATGATATTGATTTGTCTTCATCACATCTATCCCAGAATGAAGGTGGTTTCTCAAATCAATTTTCAAGTTTGAATTTGCAAAGCAGAGAGCAGAATGGTAGTGTTGGTGATTTATCAGTTTCTGATTCTAAGGGTGAACTCCAAAAGTCCTTGAACGGGCCTGCCACAGTCTGTACAATTTTGTTGCCTCGTGGTTTAATCAACTCGGGAAATCTCTGCTTTCTTAATGCGACTCTGCAGGCTCTCCTATCCTGTTCTCCTTTCGTTCAACTTCTTCATGAATTAAGAACTCGCAACATCCCCAAGATTGGCTACCCCACATTGTGTGCATTTGCTGAGTTTGTCTCGGAATTCGACGTGCCTAGTTCAAGTTCAAAGAATAAAGATACAAGTGTTGCCGAGGCTGGTAGGCCTTTTAGCCCTGCCATGTTTGAAGGTGTTCTTAAAGCTTTTACTCCAGATGTGCCAAGTACCATATCAGGCAGGCCAAGACAAGAAGATGCTCAGGAGTTTCTTAGCTTCATTATGGATCAGATGCATGATGAGTTACTTAAGCTTGAAGGACAATCCTCGTGCTTAAATGGGGGAAAATCATCTCTTGTATCTTCTGCAGAAGATGATGAATGGGAGACGGTTGGCCCAAAGAATACATCTGCAGTAACTAGAACACAGAGTTTTGTCCCTTCAGAATTGAGTGATATTTTTGGTGGACAATTAAGAAGTGTGGTGAAGGCAAGAGGAAATAAAGCTTCTGCTACTGTACAACCATATCTATTGCTGCACCTTGATATTAATCCTGAAGCTGTTCGTACTATTGAAGATGCACTTAAGTTGTTTTCTGCTCCAGAAACTCTTGAGGGATATCGAACATCAGCTGCGGGCAAGGCTGGTGTTGTGAGTGCAAGTAAATCAATAAAAATACAAAAGCTTTCAAAGATAATGATACTGCATTTGATGCGTTTTGGTTATGGAAGTCAAGGAAGCACCAAGTTGCATAAGCCTGTGCACTTTCCACTCGAATTGGTGTTTGGTCGTGAATTGCTTGTTTCTCCAACATCTGAGGTGATGTTCTTTATATATTTGATATCTAAATTATTTTCTTTACAAATCATTTATTTATTTATTTATTTTTCTGCATATTTTTTTATTTACAATCTTGTCTTGGCCCCCATGTGCATTATTATATGTGATATCCGATTTAAGGAAAGATTGCGTGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

548

Amino Acids

60.06

Weight (kDa)

6.34

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 205 - 483 6.6e-26 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 229
AciI CCGC 1 cut(s) 1301
AclWI GGATC 3 cut(s) 229, 343, 960
AcsI RAATTY 4 cut(s) 19, 181, 496, 773
AcuI CTGAAG 3 cut(s) 96, 1092, 1242
AdeI CACNNNGTG 1 cut(s) 747
AfaI GTAC 4 cut(s) 598, 895, 1185, 1234
AfiI CCNNNNNNNGG 4 cut(s) 134, 579, 731, 1624
AflII CTTAAG 2 cut(s) 974, 1250
AjiI CACGTC 1 cut(s) 781
AjuI GAANNNNNNNTTGG 2 cut(s) 1434, 1466
AluBI AGCT 9 cut(s) 52, 314, 872, 942, 979, 1172, 1226, 1298, 1351
AluI AGCT 9 cut(s) 52, 314, 872, 942, 979, 1172, 1226, 1298, 1351
Alw21I GWGCWC 1 cut(s) 1431
Alw26I GTCTC 4 cut(s) 77, 273, 772, 1046
Alw44I GTGCAC 1 cut(s) 1427
AlwI GGATC 3 cut(s) 229, 343, 960
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 6 cut(s) 251, 581, 833, 908, 1060, 1587
ApaLI GTGCAC 1 cut(s) 1427
ApeKI GCWGC 3 cut(s) 49, 1201, 1298
ApoI RAATTY 4 cut(s) 19, 181, 496, 773
AseI ATTAAT 1 cut(s) 1215
Asp700I GAANNNNTTC 2 cut(s) 861, 1227
AspS9I GGNCC 4 cut(s) 251, 581, 1061, 1588
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 7 cut(s) 102, 117, 149, 542, 569, 1162, 1498
AvaI CYCGRG 1 cut(s) 628
BaeGI GKGCMC 1 cut(s) 1431
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BauI CACGAG 2 cut(s) 612, 994
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 1431
BbvI GCAGC 3 cut(s) 61, 1188, 1285
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 4 cut(s) 77, 273, 772, 1046
BfaI CTAG 3 cut(s) 332, 786, 1086
BfmI CTRYAG 3 cut(s) 659, 1032, 1077
BfrI CTTAAG 2 cut(s) 974, 1250
BisI GCNGC 3 cut(s) 50, 1202, 1299
BlsI GCNGC 3 cut(s) 51, 1203, 1300
Bme1390I CCNGG 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 4 cut(s) 251, 581, 1061, 1588
BmiI GGNNCC 2 cut(s) 252, 1590
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 4 cut(s) 913, 948, 1234, 1368
BpiI GAAGAC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 752, 784
BpmI CTGGAG 3 cut(s) 336, 864, 1251
Bpu10I CCTNAGC 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 1298
BpuMI CCSGG 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 1215
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 41, 113, 822
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 579, 731, 1624
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 1215
BseDI CCNNGG 3 cut(s) 41, 113, 822
BseGI GGATG 1 cut(s) 720
BseJI GATNNNNATC 1 cut(s) 1215
BseLI CCNNNNNNNGG 4 cut(s) 134, 579, 731, 1624
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 3 cut(s) 750, 941, 1473
BseNI ACTGG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 1431
BseXI GCAGC 3 cut(s) 61, 1188, 1285
BsgI GTGCAG 1 cut(s) 1187
BshFI GGCC 6 cut(s) 253, 583, 835, 910, 1062, 1589
BsiHKAI GWGCWC 1 cut(s) 1431
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 1 cut(s) 1088
BslI CCNNNNNNNGG 4 cut(s) 134, 579, 731, 1624
BsmAI GTCTC 4 cut(s) 77, 273, 772, 1046
BsmBI CGTCTC 1 cut(s) 1046
BsmFI GGGAC 1 cut(s) 1088
BsnI GGCC 6 cut(s) 253, 583, 835, 910, 1062, 1589
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 1431
Bsp1407I TGTACA 2 cut(s) 596, 1183
Bsp143I GATC 4 cut(s) 221, 335, 370, 952
BspACI CCGC 1 cut(s) 1301
BspANI GGCC 6 cut(s) 253, 583, 835, 910, 1062, 1589
BspCNI CTCAG 3 cut(s) 751, 940, 1474
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 2 cut(s) 252, 1590
BspMAI CTGCAG 3 cut(s) 663, 1036, 1081
BspPI GGATC 3 cut(s) 229, 343, 960
BspTI CTTAAG 2 cut(s) 974, 1250
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 92
BsrGI TGTACA 2 cut(s) 596, 1183
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 41, 113, 822
BssMI GATC 4 cut(s) 221, 335, 370, 952
BssSI CACGAG 2 cut(s) 612, 994
BssT1I CCWWGG 1 cut(s) 41
Bst2BI CACGAG 2 cut(s) 612, 994
Bst4CI ACNGT 5 cut(s) 380, 386, 592, 1057, 1183
BstAFI CTTAAG 2 cut(s) 974, 1250
BstAPI GCANNNNNTGC 1 cut(s) 1378
BstAUI TGTACA 2 cut(s) 596, 1183
BstC8I GCNNGC 4 cut(s) 585, 663, 908, 1303
BstDEI CTNAG 5 cut(s) 552, 759, 927, 938, 1482
BstF5I GGATG 1 cut(s) 720
BstKTI GATC 4 cut(s) 224, 338, 373, 955
BstMAI GTCTC 4 cut(s) 77, 273, 772, 1046
BstMBI GATC 4 cut(s) 221, 335, 370, 952
BstMWI GCNNNNNNNGC 3 cut(s) 103, 1307, 1378
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 3 cut(s) 659, 1032, 1077
BstSLI GKGCMC 1 cut(s) 1431
BstV1I GCAGC 3 cut(s) 61, 1188, 1285
BstV2I GAAGAC 1 cut(s) 432
BsuRI GGCC 6 cut(s) 253, 583, 835, 910, 1062, 1589
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 153, 345
Cac8I GCNNGC 4 cut(s) 585, 663, 908, 1303
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 700
Cfr13I GGNCC 4 cut(s) 251, 581, 1061, 1588
Csp6I GTAC 4 cut(s) 597, 894, 1184, 1233
CspCI CAANNNNNGTGG 2 cut(s) 1425, 1460
CviAII CATG 4 cut(s) 701, 850, 962, 1594
CviQI GTAC 4 cut(s) 597, 894, 1184, 1233
DdeI CTNAG 5 cut(s) 552, 759, 927, 938, 1482
DpnI GATC 4 cut(s) 223, 337, 372, 954
DpnII GATC 4 cut(s) 221, 335, 370, 952
DraIII CACNNNGTG 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 41
Eco147I AGGCCT 1 cut(s) 835
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 3 cut(s) 1286, 1509, 1615
Eco57I CTGAAG 3 cut(s) 96, 1092, 1242
Eco88I CYCGRG 1 cut(s) 628
EcoRI GAATTC 1 cut(s) 773
EcoRV GATATC 3 cut(s) 1286, 1509, 1615
EcoT14I CCWWGG 1 cut(s) 41
EcoT22I ATGCAT 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 1046
FaeI CATG 4 cut(s) 704, 853, 965, 1597
FalI AAGNNNNNCTT 4 cut(s) 849, 881, 1233, 1265
FaqI GGGAC 1 cut(s) 1088
FatI CATG 4 cut(s) 700, 849, 961, 1593
FauI CCCGC 1 cut(s) 1294
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 50, 1202, 1299
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 3 cut(s) 50, 1202, 1299
FspBI CTAG 3 cut(s) 332, 786, 1086
GluI GCNGC 3 cut(s) 50, 1202, 1299
GsuI CTGGAG 3 cut(s) 336, 864, 1251
HaeIII GGCC 6 cut(s) 253, 583, 835, 910, 1062, 1589
HapII CCGG 1 cut(s) 113
Hin1II CATG 4 cut(s) 704, 853, 965, 1597
HindIII AAGCTT 4 cut(s) 870, 977, 1170, 1349
HinfI GANTC 2 cut(s) 548, 655
HpaII CCGG 1 cut(s) 113
HphI GGTGA 7 cut(s) 102, 117, 149, 542, 569, 1162, 1498
Hpy166II GTNNAC 4 cut(s) 230, 560, 1133, 1429
Hpy188I TCNGA 8 cut(s) 76, 351, 547, 772, 957, 1111, 1483, 1619
Hpy188III TCNNGA 8 cut(s) 630, 701, 881, 929, 1220, 1268, 1277, 1457
Hpy8I GTNNAC 4 cut(s) 230, 560, 1133, 1429
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 7 cut(s) 127, 241, 458, 851, 977, 1116, 1147
HpyCH4III ACNGT 5 cut(s) 380, 386, 592, 1057, 1183
HpyCH4IV ACGT 2 cut(s) 159, 780
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 1307, 1378
HpyF3I CTNAG 5 cut(s) 552, 759, 927, 938, 1482
HpySE526I ACGT 2 cut(s) 159, 780
Hsp92II CATG 4 cut(s) 704, 853, 965, 1597
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 221, 335, 370, 952
LmnI GCTCC 1 cut(s) 1270
Lsp1109I GCAGC 3 cut(s) 61, 1188, 1285
LweI GCATC 4 cut(s) 913, 948, 1234, 1368
MaeI CTAG 3 cut(s) 332, 786, 1086
MaeII ACGT 2 cut(s) 159, 780
MaeIII GTNAC 6 cut(s) 123, 187, 380, 415, 969, 1081
MalI GATC 4 cut(s) 223, 337, 372, 954
MboI GATC 4 cut(s) 221, 335, 370, 952
MboII GAAGA 7 cut(s) 89, 432, 689, 932, 1020, 1049, 1253
MhlI GDGCHC 1 cut(s) 1431
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 2 cut(s) 374, 1499
MnlI CCTC 7 cut(s) 406, 621, 817, 1003, 1154, 1273, 1477
Mph1103I ATGCAT 1 cut(s) 963
MroXI GAANNNNTTC 2 cut(s) 861, 1227
MslI CAYNNNNRTG 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 1298
MspCI CTTAAG 2 cut(s) 974, 1250
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 103, 1307, 1378
NciI CCSGG 1 cut(s) 114
NdeII GATC 4 cut(s) 221, 335, 370, 952
NlaIII CATG 4 cut(s) 704, 853, 965, 1597
NlaIV GGNNCC 2 cut(s) 252, 1590
NmeAIII GCCGAG 2 cut(s) 279, 847
NmuCI GTSAC 2 cut(s) 123, 380
NsiI ATGCAT 1 cut(s) 963
PagI TCATGA 1 cut(s) 700
PceI AGGCCT 1 cut(s) 835
PdmI GAANNNNTTC 2 cut(s) 861, 1227
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 3 cut(s) 51, 1203, 1300
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PshBI ATTAAT 1 cut(s) 1215
PspN4I GGNNCC 2 cut(s) 252, 1590
PspPI GGNCC 4 cut(s) 251, 581, 1061, 1588
PstI CTGCAG 3 cut(s) 663, 1036, 1081
PstNI CAGNNNCTG 1 cut(s) 545
PsyI GACNNNGTC 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 1298
RsaI GTAC 4 cut(s) 598, 895, 1185, 1234
RsaNI GTAC 4 cut(s) 597, 894, 1184, 1233
RseI CAYNNNNRTG 1 cut(s) 399
SatI GCNGC 3 cut(s) 50, 1202, 1299
Sau3AI GATC 4 cut(s) 221, 335, 370, 952
Sau96I GGNCC 4 cut(s) 251, 581, 1061, 1588
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 1431
SfaNI GCATC 4 cut(s) 913, 948, 1234, 1368
SfcI CTRYAG 3 cut(s) 659, 1032, 1077
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 3 cut(s) 974, 1250, 1277
SmoI CTYRAG 3 cut(s) 974, 1250, 1277
SseBI AGGCCT 1 cut(s) 835
SsiI CCGC 1 cut(s) 1301
SspMI CTAG 3 cut(s) 332, 786, 1086
StuI AGGCCT 1 cut(s) 835
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 5 cut(s) 380, 386, 592, 1057, 1183
TaiI ACGT 2 cut(s) 162, 783
TaqI TCGA 4 cut(s) 176, 777, 1288, 1440
TatI WGTACW 2 cut(s) 596, 1183
TfiI GAWTC 1 cut(s) 548
TscAI CASTG 2 cut(s) 153, 345
TseFI GTSAC 2 cut(s) 123, 380
TseI GCWGC 3 cut(s) 49, 1201, 1298
Tsp45I GTSAC 2 cut(s) 123, 380
TspDTI ATGAA 8 cut(s) 46, 309, 432, 477, 689, 717, 934, 1059
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 153, 345
Tth111I GACNNNGTC 1 cut(s) 88
Vha464I CTTAAG 2 cut(s) 974, 1250
VneI GTGCAC 1 cut(s) 1427
VspI ATTAAT 1 cut(s) 1215
XapI RAATTY 4 cut(s) 19, 181, 496, 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 229
XmnI GAANNNNTTC 2 cut(s) 861, 1227
XspI CTAG 3 cut(s) 332, 786, 1086
Zsp2I ATGCAT 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.