MD03G1005700.v1.1

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004745
Physical Location & Seq
Reverse (-)
465874 .. 471326
5453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1005700.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGAGGAGCAGTTCGTGCTCAAAGTCCCACCTTCTGTTGCAGAGCGACTGGACCGCCTTCTGGGCGAAAACGCTTCTACCGACGACAAGTCGTTGGATTTGTCATTCGGAGAGGATGGCAGGACTGGCACATTTGTCATTGGCAATGACCATTTCCCCGCATCACTGTTTGATCTTCCTTGTGTTGTAGAGTCCTTCAAAACATATGATGATAGTGTGTTAATTAAGACAGCAGATATTGGTCAGATGATTATGGTTAGAGACCCGAATGATCCAGCTCCAGATACAGTGGAGTACAGGCATGGCCTCACCCCTCCAATGAGGGATGCTCGAAAGCGAAGATTTCGCAGGGAACCTGATCTAAATCCTGAGCTTGTCCGGCGTGTTGAGGAAGACCTACTGAATATTAGTATAAGCGGACCGGCTGATAATATTGATATCCTCACATATTATGTTTCATTACTTTCCTGTTATCATTTTGTGAAAAAGGCAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000123 GO:0000428 GO:0001067 GO:0001932 GO:0001933 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005737 GO:0005794 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006469 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010563 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0012505 GO:0014070 GO:0016043 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019899 GO:0022607 GO:0023052 GO:0030518 GO:0030520 GO:0030522 GO:0030880 GO:0031056 GO:0031057 GO:0031248 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032774 GO:0032870 GO:0032991 GO:0033043 GO:0033044 GO:0033276 GO:0033673 GO:0033993 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0034708 GO:0035035 GO:0035065 GO:0035067 GO:0035097 GO:0035257 GO:0042221 GO:0042325 GO:0042326 GO:0042809 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043401 GO:0043549 GO:0043933 GO:0044085 GO:0044092 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044665 GO:0044798 GO:0045859 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045936 GO:0045944 GO:0046483 GO:0046966 GO:0046982 GO:0046983 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051123 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051338 GO:0051348 GO:0051427 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070461 GO:0070887 GO:0070897 GO:0071310 GO:0071339 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901983 GO:1901984 GO:1902275 GO:1902493 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1990234 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TAFII55_N

Protein Analysis

166

Amino Acids

18.7

Weight (kDa)

4.8

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAFII55_N PF04658 1 - 131 3e-31 TAFII55 protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 55, 159, 417
AclWI GGATC 1 cut(s) 266
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 1 cut(s) 199
AhdI GACNNNNNGTC 1 cut(s) 88
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 2 cut(s) 278, 373
AluI AGCT 2 cut(s) 278, 373
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 266
AoxI GGCC 1 cut(s) 304
AspS9I GGNCC 2 cut(s) 52, 419
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 2 cut(s) 52, 419
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 21
BccI CCATC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 52, 419
BmeRI GACNNNNNGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 52, 419
BmiI GGNNCC 1 cut(s) 354
BmsI GCATC 2 cut(s) 170, 316
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 313, 345
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 255
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 2 cut(s) 54, 130
Bse3DI GCAATG 1 cut(s) 151
Bse8I GATNNNNATC 1 cut(s) 363
BseGI GGATG 2 cut(s) 121, 331
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 54, 130
BseRI GAGGAG 1 cut(s) 20
BshFI GGCC 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 21
BsiSI CCGG 2 cut(s) 379, 422
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 11
BsnI GGCC 1 cut(s) 306
Bso31I GGTCTC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 3 cut(s) 172, 271, 358
BspACI CCGC 3 cut(s) 55, 159, 417
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 354
BspPI GGATC 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 2 cut(s) 54, 130
BssAI RCCGGY 1 cut(s) 421
BssMI GATC 3 cut(s) 172, 271, 358
Bst4CI ACNGT 2 cut(s) 168, 289
BstAPI GCANNNNNTGC 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 121, 331
BstKTI GATC 3 cut(s) 175, 274, 361
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 3 cut(s) 172, 271, 358
BstMWI GCNNNNNNNGC 4 cut(s) 16, 63, 126, 379
BstV2I GAAGAC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 306
BtsCI GGATG 2 cut(s) 121, 331
BtsIMutI CAGTG 2 cut(s) 164, 294
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 52, 419
CpoI CGGWCCG 1 cut(s) 419
Csp6I GTAC 1 cut(s) 295
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 1 cut(s) 302
CviJI RGCY 4 cut(s) 278, 306, 373, 425
CviKI_1 RGCY 4 cut(s) 278, 306, 373, 425
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 369
DpnI GATC 3 cut(s) 174, 273, 360
DpnII GATC 3 cut(s) 172, 271, 358
DriI GACNNNNNGTC 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 88
Eco31I GGTCTC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 52, 419
EcoRV GATATC 1 cut(s) 439
FaeI CATG 1 cut(s) 305
FaiI YATR 8 cut(s) 205, 207, 254, 303, 413, 448, 453, 496
FaqI GGGAC 1 cut(s) 11
FatI CATG 1 cut(s) 301
FauI CCCGC 1 cut(s) 166
FauNDI CATATG 1 cut(s) 205
FokI GGATG 2 cut(s) 128, 338
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 1 cut(s) 306
HapII CCGG 2 cut(s) 379, 422
Hin1II CATG 1 cut(s) 305
HinfI GANTC 1 cut(s) 191
HpaII CCGG 2 cut(s) 379, 422
HphI GGTGA 1 cut(s) 301
Hpy188I TCNGA 2 cut(s) 110, 246
Hpy188III TCNNGA 2 cut(s) 281, 368
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 3 cut(s) 42, 68, 205
HpyCH4III ACNGT 2 cut(s) 168, 289
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 63, 126, 379
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 1 cut(s) 305
Kzo9I GATC 3 cut(s) 172, 271, 358
LmnI GCTCC 2 cut(s) 7, 283
LweI GCATC 2 cut(s) 170, 316
MalI GATC 3 cut(s) 174, 273, 360
MboI GATC 3 cut(s) 172, 271, 358
MboII GAAGA 3 cut(s) 167, 351, 404
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 1 cut(s) 222
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 6 cut(s) 106, 315, 317, 324, 382, 452
MseI TTAA 2 cut(s) 221, 225
MspI CCGG 2 cut(s) 379, 422
MwoI GCNNNNNNNGC 4 cut(s) 16, 63, 126, 379
NdeI CATATG 1 cut(s) 205
NdeII GATC 3 cut(s) 172, 271, 358
NlaIII CATG 1 cut(s) 305
NlaIV GGNNCC 1 cut(s) 354
PacI TTAATTAA 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 52, 419
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 221, 225
Sau3AI GATC 3 cut(s) 172, 271, 358
Sau96I GGNCC 2 cut(s) 52, 419
SchI GAGTC 1 cut(s) 200
SduI GDGCHC 1 cut(s) 21
SetI ASST 5 cut(s) 34, 280, 358, 375, 399
SfaNI GCATC 2 cut(s) 170, 316
SinI GGWCC 2 cut(s) 52, 419
Sse9I AATT 1 cut(s) 222
SsiI CCGC 3 cut(s) 55, 159, 417
SspI AATATT 2 cut(s) 406, 433
TaaI ACNGT 2 cut(s) 168, 289
TaqI TCGA 1 cut(s) 331
TasI AATT 1 cut(s) 222
TatI WGTACW 1 cut(s) 294
Tru1I TTAA 2 cut(s) 221, 225
Tru9I TTAA 2 cut(s) 221, 225
TscAI CASTG 2 cut(s) 171, 294
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 2 cut(s) 171, 294
VpaK11BI GGWCC 2 cut(s) 52, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.