Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002963

Physical Location & Seq

Genomic Coordinates Reverse (-)

1208384 .. 1209896

Gene Structure 1513 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1015100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1164 bp

ATGGGAGCTGGGGGGAACATGGTCACCAAAACCAAGTCTAAAAAGCAAGAAGCCACCCACCAAAAAAGGGTGCCAGTCTCAAAACCTCCATTCACTCTAAGCCAAATCAAGAAAGCTATCCCTCCCCATTGCTTCAACCGCTCTCTCCTCCGCTCCTTCTCCTATCTCCTCTACGACCTCGCCTTATCCTTCCTCTTCTACCACATCGCCACCTCATACTTCCACCTCCTCCCTCACCCCCTCCTCTCCTACATCTCCTGGCCTCTCTATTGGATCCTCCAAGGCTGCACCCTCACCGGCGTGTGGGTTATCGCACACGAGTGCGGCCACCATGCCTTCAGTGACTACCAAGTAGTTGATGACACTGTCGGCCTTGTCCTCCACTCATTTCTCCTAACCCCTTACTTCTCCTGGAAATACAGCCACCGCCGCCACCACTCCAACACGGCGTCCCTCGAACGGGATGAAGTATTTGTTCCAAAACCTAAATCCAAAATTCCGTTTTACAACAAGTACTTCAACAACCCTCCAGGCAGGATCTTGAGTATTTTGGTAACACTCACACTCGGTTGGCCTTTGTACTTGGCGTTCAACGCCTCTGGCAGGGACTACGACCGGTTTGCGTGCCACTACGATCCCAAAGGTCCAATATTTTCGACCCGTGAAAGGCTTCAAATTTACATTTCTGATCTTGGGATTGTGGCCGTAACTTATGTGCTCTACCGCATTGCAATGGCCAAAGGGTTCGTTTGGCTTACTTGTATTTATGGAGTCCCATTGCTTGTAGTTAACGGGTTTCTTGTGTTGATCACGTACTTGCATCACACACACTCCTCGCTGCCGCACTTCGACGCGTCGGAATGGGACTGGTTGCGAGGGGCTTTGTCGACGGTGGACAGAGATTTTGGGGTGCTTAATAAGGTTTTCCACAATATAACAGATTCGCATGTGGTTCACCATCTTTTCTCTACAATGCCACATTACCATGCAGTGGAGGCAACAGAAGCAGTGAAGCCTGTCTTGGGAGAGTACTATAGTTTCGATGGGACTCCGGTTTACAAGGCCTTGTGGAGGGAGGCTAAAGAGTGCCTTTATGTTGAGCTGGATGATGAGGATAATGGTGGTGCTAAAAAAGGTGTTTTTTGGTACAAGAACAAGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016021 GO:0016491 GO:0016705 GO:0016709 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050183 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko01040 ko01212 map01040 map01212

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

388

Amino Acids

44.56

Weight (kDa)

8.89

Isoelectric Point (pI)

40.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	14 - 64	1.2e-07	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	86 - 345	3.7e-29	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12120 AT3G12120
fragaria_vesca	FvH4_3g44270 FvH4_6g22400
malus_domestica	MD03G1015100.v1.1 MD12G1057600.v1.1 MD14G1058300.v1.1
prunus_persica	Prupe.7G076500_v2.0.a1 Prupe.7G076500_v2.0.a1
pyrus_communis	pycom03g01260 pycom12g05120 pycom14g04710
rosa_chinensis	RchiOBHm_Chr3g0479761 RchiOBHm_Chr5g0080581
rosa_laevigata	RLG00000023544 RLG00000036873
rosa_multiflora	Rmu_sc0002380.1_g000002 Rmu_sc0003755.1_g000012
rosa_roxburghii	Rroxscaffold_1G00002780 Rroxscaffold_6G00402030
rosa_rugosa	Rorug03G0178000 Rorug03G0178000 Rorug03G0178000 Rorug03G0178100 Rorug03G0178200 Rorug05G0470600
rosa_samantha	Rh3AG228900 Rh3BG262000 Rh3CG258900 Rh3DG255000 Rh5AG524000 Rh5BG546200 Rh5CG569400 Rh5DG556400
rosa_wichuraiana	Rw0G015310 Rw3G020450 Rw3G020630 Rw5G048610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	70
AccB7I	CCANNNNNTGG	1 cut(s)	991
AccBSI	CCGCTC	2 cut(s)	141, 153
AccI	GTMKAC	1 cut(s)	887
AccII	CGCG	1 cut(s)	854
AciI	CCGC	7 cut(s)	139, 151, 324, 427, 430, 724, 842
AclWI	GGATC	4 cut(s)	268, 281, 545, 629
AcoI	YGGCCR	3 cut(s)	325, 702, 735
AcsI	RAATTY	2 cut(s)	495, 675
AcuI	CTGAAG	1 cut(s)	322
AcyI	GRCGYC	1 cut(s)	449
AfaI	GTAC	5 cut(s)	515, 581, 815, 1031, 1148
AfiI	CCNNNNNNNGG	9 cut(s)	67, 303, 459, 460, 603, 666, 991, 1022, 1071
AflIII	ACRYGT	1 cut(s)	852
AgeI	ACCGGT	1 cut(s)	615
AgsI	TTSAA	4 cut(s)	136, 520, 592, 674
AjnI	CCWGG	3 cut(s)	257, 410, 529
AjuI	GAANNNNNNNTTGG	2 cut(s)	1004, 1036
AleI	CACNNNNGTG	2 cut(s)	299, 319
AluBI	AGCT	3 cut(s)	8, 116, 1102
AluI	AGCT	3 cut(s)	8, 116, 1102
Alw21I	GWGCWC	1 cut(s)	720
Alw26I	GTCTC	1 cut(s)	82
AlwI	GGATC	4 cut(s)	268, 281, 545, 629
AoxI	GGCC	7 cut(s)	260, 325, 370, 572, 702, 735, 1062
ApeKI	GCWGC	2 cut(s)	285, 838
ApoI	RAATTY	2 cut(s)	495, 675
ArsI	GACNNNNNNTTYG	2 cut(s)	887, 919
AsiGI	ACCGGT	1 cut(s)	615
Asp700I	GAANNNNTTC	1 cut(s)	669
AspS9I	GGNCC	1 cut(s)	644
AsuHPI	GGTGA	4 cut(s)	16, 227, 286, 947
AvaII	GGWCC	1 cut(s)	644
BalI	TGGCCA	1 cut(s)	737
BamHI	GGATCC	1 cut(s)	273
BanI	GGYRCC	1 cut(s)	70
BauI	CACGAG	1 cut(s)	317
Bbv12I	GWGCWC	1 cut(s)	720
BbvI	GCAGC	2 cut(s)	272, 825
BccI	CCATC	2 cut(s)	966, 1037
BceAI	ACGGC	2 cut(s)	462, 689
BcgI	CGANNNNNNTGC	2 cut(s)	602, 636
BciT130I	CCWGG	3 cut(s)	259, 412, 531
BclI	TGATCA	1 cut(s)	807
BcoDI	GTCTC	1 cut(s)	82
BfmI	CTRYAG	1 cut(s)	1033
BisI	GCNGC	5 cut(s)	286, 325, 430, 839, 842
BlsI	GCNGC	5 cut(s)	287, 326, 431, 840, 843
BmcAI	AGTACT	2 cut(s)	515, 1031
Bme1390I	CCNGG	3 cut(s)	259, 412, 531
Bme18I	GGWCC	1 cut(s)	644
BmgT120I	GGNCC	1 cut(s)	644
BmiI	GGNNCC	2 cut(s)	72, 275
BmrFI	CCNGG	3 cut(s)	259, 412, 531
BmsI	GCATC	1 cut(s)	829
BpmI	CTGGAG	1 cut(s)	513
BpuEI	CTTGAG	1 cut(s)	562
BsaAI	YACGTR	1 cut(s)	813
BsaHI	GRCGYC	1 cut(s)	449
BsaJI	CCNNGG	1 cut(s)	280
BsaWI	WCCGGW	2 cut(s)	615, 1051
BsaXI	ACNNNNNCTCC	2 cut(s)	815, 845
Bsc4I	CCNNNNNNNGG	9 cut(s)	67, 303, 459, 460, 603, 666, 991, 1022, 1071
Bse118I	RCCGGY	2 cut(s)	296, 615
Bse1I	ACTGG	2 cut(s)	74, 872
Bse3DI	GCAATG	4 cut(s)	127, 726, 738, 776
BseBI	CCWGG	3 cut(s)	259, 412, 531
BseDI	CCNNGG	1 cut(s)	280
BseGI	GGATG	2 cut(s)	469, 1111
BseLI	CCNNNNNNNGG	9 cut(s)	67, 303, 459, 460, 603, 666, 991, 1022, 1071
BseMI	GCAATG	4 cut(s)	127, 726, 738, 776
BseNI	ACTGG	2 cut(s)	74, 872
BseRI	GAGGAG	5 cut(s)	137, 158, 218, 233, 823
BseXI	GCAGC	2 cut(s)	272, 825
BseYI	CCCAGC	1 cut(s)	8
BsgI	GTGCAG	1 cut(s)	271
Bsh1236I	CGCG	1 cut(s)	854
Bsh1285I	CGRYCG	1 cut(s)	616
BshFI	GGCC	7 cut(s)	262, 327, 372, 574, 704, 737, 1064
BshNI	GGYRCC	1 cut(s)	70
BshTI	ACCGGT	1 cut(s)	615
BsiEI	CGRYCG	1 cut(s)	616
BsiHKAI	GWGCWC	1 cut(s)	720
BsiSI	CCGG	3 cut(s)	297, 616, 1052
BslFI	GGGAC	5 cut(s)	436, 620, 758, 878, 1060
BslI	CCNNNNNNNGG	9 cut(s)	67, 303, 459, 460, 603, 666, 991, 1022, 1071
BsmAI	GTCTC	1 cut(s)	82
BsmFI	GGGAC	5 cut(s)	436, 620, 758, 878, 1060
BsnI	GGCC	7 cut(s)	262, 327, 372, 574, 704, 737, 1064
Bsp1286I	GDGCHC	1 cut(s)	720
Bsp143I	GATC	5 cut(s)	273, 537, 634, 688, 807
BspACI	CCGC	7 cut(s)	139, 151, 324, 427, 430, 724, 842
BspANI	GGCC	7 cut(s)	262, 327, 372, 574, 704, 737, 1064
BspFNI	CGCG	1 cut(s)	854
BspLI	GGNNCC	2 cut(s)	72, 275
BspPI	GGATC	4 cut(s)	268, 281, 545, 629
BspT107I	GGYRCC	1 cut(s)	70
BsrBI	CCGCTC	2 cut(s)	141, 153
BsrDI	GCAATG	4 cut(s)	127, 726, 738, 776
BsrFI	RCCGGY	2 cut(s)	296, 615
BsrI	ACTGG	2 cut(s)	74, 872
BssAI	RCCGGY	2 cut(s)	296, 615
BssECI	CCNNGG	1 cut(s)	280
BssMI	GATC	5 cut(s)	273, 537, 634, 688, 807
BssNI	GRCGYC	1 cut(s)	449
BssSI	CACGAG	1 cut(s)	317
BssT1I	CCWWGG	1 cut(s)	280
Bst2BI	CACGAG	1 cut(s)	317
Bst2UI	CCWGG	3 cut(s)	259, 412, 531
Bst4CI	ACNGT	2 cut(s)	367, 892
Bst6I	CTCTTC	1 cut(s)	200
BstACI	GRCGYC	1 cut(s)	449
BstBAI	YACGTR	1 cut(s)	813
BstC8I	GCNNGC	1 cut(s)	625
BstDEI	CTNAG	1 cut(s)	98
BstEII	GGTNACC	1 cut(s)	22
BstENI	CCTNNNNNAGG	2 cut(s)	601, 1069
BstF5I	GGATG	2 cut(s)	469, 1111
BstFNI	CGCG	1 cut(s)	854
BstKTI	GATC	5 cut(s)	276, 540, 637, 691, 810
BstMAI	GTCTC	1 cut(s)	82
BstMBI	GATC	5 cut(s)	273, 537, 634, 688, 807
BstMCI	CGRYCG	1 cut(s)	616
BstMWI	GCNNNNNNNGC	5 cut(s)	138, 429, 593, 995, 1004
BstNI	CCWGG	3 cut(s)	259, 412, 531
BstNSI	RCATGY	1 cut(s)	950
BstPI	GGTNACC	1 cut(s)	22
BstSCI	CCNGG	3 cut(s)	257, 410, 529
BstSFI	CTRYAG	1 cut(s)	1033
BstUI	CGCG	1 cut(s)	854
BstV1I	GCAGC	2 cut(s)	272, 825
BstX2I	RGATCY	2 cut(s)	273, 537
BstYI	RGATCY	2 cut(s)	273, 537
BsuRI	GGCC	7 cut(s)	262, 327, 372, 574, 704, 737, 1064
BtgZI	GCGATG	1 cut(s)	190
BtsCI	GGATG	2 cut(s)	469, 1111
BtsI	GCAGTG	2 cut(s)	996, 1014
BtsIMutI	CAGTG	4 cut(s)	346, 363, 996, 1014
Cac8I	GCNNGC	1 cut(s)	625
Cfr10I	RCCGGY	2 cut(s)	296, 615
Cfr13I	GGNCC	1 cut(s)	644
CseI	GACGC	3 cut(s)	438, 843, 860
Csp6I	GTAC	5 cut(s)	514, 580, 814, 1030, 1147
CspAI	ACCGGT	1 cut(s)	615
CviAII	CATG	4 cut(s)	19, 332, 947, 986
CviQI	GTAC	5 cut(s)	514, 580, 814, 1030, 1147
DdeI	CTNAG	1 cut(s)	98
DpnI	GATC	5 cut(s)	275, 539, 636, 690, 809
DpnII	GATC	5 cut(s)	273, 537, 634, 688, 807
EaeI	YGGCCR	3 cut(s)	325, 702, 735
Eam1104I	CTCTTC	1 cut(s)	200
EarI	CTCTTC	1 cut(s)	200
Eco130I	CCWWGG	1 cut(s)	280
Eco147I	AGGCCT	1 cut(s)	1064
Eco47I	GGWCC	1 cut(s)	644
Eco57I	CTGAAG	1 cut(s)	322
Eco91I	GGTNACC	1 cut(s)	22
EcoNI	CCTNNNNNAGG	2 cut(s)	601, 1069
EcoO65I	GGTNACC	1 cut(s)	22
EcoRII	CCWGG	3 cut(s)	257, 410, 529
EcoT14I	CCWWGG	1 cut(s)	280
ErhI	CCWWGG	1 cut(s)	280
FaeI	CATG	4 cut(s)	22, 335, 950, 989
FalI	AAGNNNNNCTT	4 cut(s)	1004, 1036, 1074, 1106
FaqI	GGGAC	5 cut(s)	436, 620, 758, 878, 1060
FatI	CATG	4 cut(s)	18, 331, 946, 985
FbaI	TGATCA	1 cut(s)	807
FblI	GTMKAC	1 cut(s)	887
Fnu4HI	GCNGC	5 cut(s)	286, 325, 430, 839, 842
FokI	GGATG	2 cut(s)	476, 1118
Fsp4HI	GCNGC	5 cut(s)	286, 325, 430, 839, 842
GluI	GCNGC	5 cut(s)	286, 325, 430, 839, 842
GsaI	CCCAGC	1 cut(s)	12
GsuI	CTGGAG	1 cut(s)	513
HaeIII	GGCC	7 cut(s)	262, 327, 372, 574, 704, 737, 1064
HapII	CCGG	3 cut(s)	297, 616, 1052
HgaI	GACGC	3 cut(s)	438, 843, 860
Hin1I	GRCGYC	1 cut(s)	449
Hin1II	CATG	4 cut(s)	22, 335, 950, 989
HincII	GTYRAC	2 cut(s)	790, 888
HindII	GTYRAC	2 cut(s)	790, 888
HinfI	GANTC	3 cut(s)	771, 941, 1048
HpaI	GTTAAC	1 cut(s)	790
HpaII	CCGG	3 cut(s)	297, 616, 1052
HphI	GGTGA	4 cut(s)	16, 227, 286, 947
Hpy166II	GTNNAC	5 cut(s)	790, 888, 895, 955, 1057
Hpy188I	TCNGA	2 cut(s)	688, 859
Hpy188III	TCNNGA	2 cut(s)	109, 541
Hpy8I	GTNNAC	5 cut(s)	790, 888, 895, 955, 1057
Hpy99I	CGWCG	3 cut(s)	854, 859, 892
HpyAV	CCTTC	3 cut(s)	166, 199, 346
HpyCH4III	ACNGT	2 cut(s)	367, 892
HpyCH4IV	ACGT	1 cut(s)	812
HpyCH4V	TGCA	4 cut(s)	288, 731, 820, 989
HpyF10VI	GCNNNNNNNGC	5 cut(s)	138, 429, 593, 995, 1004
HpyF3I	CTNAG	1 cut(s)	98
HpySE526I	ACGT	1 cut(s)	812
Hsp92I	GRCGYC	1 cut(s)	449
Hsp92II	CATG	4 cut(s)	22, 335, 950, 989
Ksp22I	TGATCA	1 cut(s)	807
KspAI	GTTAAC	1 cut(s)	790
Kzo9I	GATC	5 cut(s)	273, 537, 634, 688, 807
LmnI	GCTCC	2 cut(s)	5, 158
Lsp1109I	GCAGC	2 cut(s)	272, 825
LweI	GCATC	1 cut(s)	829
MaeII	ACGT	1 cut(s)	812
MaeIII	GTNAC	4 cut(s)	22, 341, 553, 706
MalI	GATC	5 cut(s)	275, 539, 636, 690, 809
MbiI	CCGCTC	2 cut(s)	141, 153
MboI	GATC	5 cut(s)	273, 537, 634, 688, 807
MboII	GAAGA	1 cut(s)	187
MflI	RGATCY	2 cut(s)	273, 537
MhlI	GDGCHC	1 cut(s)	720
MlsI	TGGCCA	1 cut(s)	737
MluCI	AATT	2 cut(s)	495, 675
MluI	ACGCGT	1 cut(s)	852
MluNI	TGGCCA	1 cut(s)	737
MlyI	GAGTC	2 cut(s)	780, 1042
MmeI	TCCRAC	2 cut(s)	465, 837
Mox20I	TGGCCA	1 cut(s)	737
MroXI	GAANNNNTTC	1 cut(s)	669
MscI	TGGCCA	1 cut(s)	737
MseI	TTAA	3 cut(s)	789, 915, 1162
MslI	CAYNNNNRTG	4 cut(s)	299, 319, 731, 984
Msp20I	TGGCCA	1 cut(s)	737
MspI	CCGG	3 cut(s)	297, 616, 1052
MspR9I	CCNGG	3 cut(s)	259, 412, 531
MvaI	CCWGG	3 cut(s)	259, 412, 531
MvnI	CGCG	1 cut(s)	854
MwoI	GCNNNNNNNGC	5 cut(s)	138, 429, 593, 995, 1004
NdeII	GATC	5 cut(s)	273, 537, 634, 688, 807
NlaIII	CATG	4 cut(s)	22, 335, 950, 989
NlaIV	GGNNCC	2 cut(s)	72, 275
NmuCI	GTSAC	2 cut(s)	22, 341
NspI	RCATGY	1 cut(s)	950
OliI	CACNNNNGTG	2 cut(s)	299, 319
PceI	AGGCCT	1 cut(s)	1064
PdmI	GAANNNNTTC	1 cut(s)	669
PfeI	GAWTC	1 cut(s)	941
PflFI	GACNNNGTC	1 cut(s)	365
PflMI	CCANNNNNTGG	1 cut(s)	991
PfoI	TCCNGGA	1 cut(s)	410
PinAI	ACCGGT	1 cut(s)	615
PkrI	GCNGC	5 cut(s)	287, 326, 431, 840, 843
PleI	GAGTC	2 cut(s)	779, 1042
PpsI	GAGTC	2 cut(s)	779, 1042
Ppu21I	YACGTR	1 cut(s)	813
Psp6I	CCWGG	3 cut(s)	257, 410, 529
PspEI	GGTNACC	1 cut(s)	22
PspFI	CCCAGC	1 cut(s)	8
PspGI	CCWGG	3 cut(s)	257, 410, 529
PspN4I	GGNNCC	2 cut(s)	72, 275
PspPI	GGNCC	1 cut(s)	644
PsrI	GAACNNNNNNTAC	2 cut(s)	572, 604
PsuI	RGATCY	2 cut(s)	273, 537
PsyI	GACNNNGTC	1 cut(s)	365
RsaI	GTAC	5 cut(s)	515, 581, 815, 1031, 1148
RsaNI	GTAC	5 cut(s)	514, 580, 814, 1030, 1147
RseI	CAYNNNNRTG	4 cut(s)	299, 319, 731, 984
SalI	GTCGAC	1 cut(s)	886
SaqAI	TTAA	3 cut(s)	789, 915, 1162
SatI	GCNGC	5 cut(s)	286, 325, 430, 839, 842
Sau3AI	GATC	5 cut(s)	273, 537, 634, 688, 807
Sau96I	GGNCC	1 cut(s)	644
ScaI	AGTACT	2 cut(s)	515, 1031
SchI	GAGTC	2 cut(s)	780, 1042
ScrFI	CCNGG	3 cut(s)	259, 412, 531
SduI	GDGCHC	1 cut(s)	720
SfaNI	GCATC	1 cut(s)	829
SfcI	CTRYAG	1 cut(s)	1033
SgrAI	CRCCGGYG	1 cut(s)	296
SinI	GGWCC	1 cut(s)	644
SmiMI	CAYNNNNRTG	4 cut(s)	299, 319, 731, 984
SmlI	CTYRAG	1 cut(s)	541
SmoI	CTYRAG	1 cut(s)	541
Sse9I	AATT	2 cut(s)	495, 675
SseBI	AGGCCT	1 cut(s)	1064
SsiI	CCGC	7 cut(s)	139, 151, 324, 427, 430, 724, 842
SspI	AATATT	1 cut(s)	651
StuI	AGGCCT	1 cut(s)	1064
StyD4I	CCNGG	3 cut(s)	257, 410, 529
StyI	CCWWGG	1 cut(s)	280
TaaI	ACNGT	2 cut(s)	367, 892
TaiI	ACGT	1 cut(s)	815
TaqI	TCGA	5 cut(s)	456, 656, 849, 887, 1041
TasI	AATT	2 cut(s)	495, 675
TatI	WGTACW	3 cut(s)	513, 579, 1029
TauI	GCSGC	3 cut(s)	327, 432, 844
TfiI	GAWTC	1 cut(s)	941
Tru1I	TTAA	3 cut(s)	789, 915, 1162
Tru9I	TTAA	3 cut(s)	789, 915, 1162
TscAI	CASTG	4 cut(s)	346, 370, 996, 1014
TseFI	GTSAC	2 cut(s)	22, 341
TseI	GCWGC	2 cut(s)	285, 838
Tsp45I	GTSAC	2 cut(s)	22, 341
TspDTI	ATGAA	1 cut(s)	480
TspGWI	ACGGA	1 cut(s)	489
TspRI	CASTG	4 cut(s)	346, 370, 996, 1014
Tth111I	GACNNNGTC	1 cut(s)	365
Van91I	CCANNNNNTGG	1 cut(s)	991
VpaK11BI	GGWCC	1 cut(s)	644
XagI	CCTNNNNNAGG	2 cut(s)	601, 1069
XapI	RAATTY	2 cut(s)	495, 675
XceI	RCATGY	1 cut(s)	950
XmiI	GTMKAC	1 cut(s)	887
XmnI	GAANNNNTTC	1 cut(s)	669
ZrmI	AGTACT	2 cut(s)	515, 1031

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.