Rw3G020630

Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002963
Physical Location & Seq
Forward (+)
24312434 .. 24317798
5365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020630.1

Sequence Viewer

Length: 1041 bp
ATGGGTGGTGGTGGAAGAATGTCTGCACCCCCAACTTGCAAGAATGCAGAAACTGACAACATCAAGCGAGTGCCACACCTGAAACCTCCATTCACTCTCGGCCAAATCCGCCACTGCCCACCTCACTGTTTCCAGCGCTCCGTCGTCCACTCTTTCTCCTATGTCGTCTATGACCTTGCAATTGCCTCCATCTTTTATTACATTGCTTCAAATTACATCAACCACCTCTCGACACCTCTCTCATTCTTGGCATGGCCAATATACTGGGCTCTTCAGGGCTGTGTCCTAACTGGTGTCTGGGTCATTGCACATGAGTGTGGTCACCATGCCTTTAATTATCAATGGCTCGATGACACAGTTGGCCTAATCCTGCACTCCTGTCTTCTTGTCCCCTACTTCTCTTGGAAGTATAGCCATCGCCGCCACCATTCTAATACAGGTTCCCTTGAGCGAGATGAAGTCTTTGTCCCAAAACAGAAGTCTGCTATGGGATGGCCCTATGACGGCTTTGCTTGCCACTACCATCCATATGGCCCTATCTACTCCGATCGTGAACGTTTGCAGATATACATTTCGGATGCTGGTGTTCTCGCAGTCTGTTATGGGCTTTACCGACTTGCTGTTGCAAAAGGGCTTTCCTGGGTCTTGTGCTTCTATGGAGGTCCCTTGTTGGTGGTGAATGCGTTTTTGGTTTTAATCACATTTTTGCAGCACACCCACCCTTCGTTGCCACACTATGATTCATCTGAATGGGACTGGTTGAGAGGAGCCTTGGCAACTGTTGACAGAGACTATGGAATCTTGAACAAGGTCTTCCATAACATCACAGATACTCATGTCGCTCACCATTTGTTCTCAACCATGCCACATTATCACGCAATGGAGGCCACAAAGGCAATCAAGCCGATATTGGGCGAGTATTATCAGTTCGATGGGACTCCATTTTACAAAGCAATGTGGAGGGAAGCAAAGGAGTGCATCTATGTTGAGCCAGATGAAGTTGATGAGAAAGGTGTCTTCTGGTACAAGAATAAGCTGTGA

Protein Analysis

346

Amino Acids

39.8

Weight (kDa)

7.09

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 84 - 309 7.8e-26 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 109, 421
AclI AACGTT 1 cut(s) 556
AcoI YGGCCR 2 cut(s) 100, 254
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 1025
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 503, 911
AgsI TTSAA 2 cut(s) 210, 805
AjnI CCWGG 1 cut(s) 638
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 1 cut(s) 1036
AluI AGCT 1 cut(s) 1036
Alw26I GTCTC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 53
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 6 cut(s) 100, 254, 361, 494, 532, 885
ApeKI GCWGC 1 cut(s) 709
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 3 cut(s) 495, 533, 662
AsuHPI GGTGA 3 cut(s) 314, 688, 836
AvaII GGWCC 1 cut(s) 662
BaeI ACNNNNGTAYC 2 cut(s) 822, 855
BalI TGGCCA 1 cut(s) 256
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 3 cut(s) 374, 805, 1009
BbvI GCAGC 1 cut(s) 721
BccI CCATC 5 cut(s) 197, 423, 486, 531, 926
BceAI ACGGC 1 cut(s) 520
BciT130I CCWGG 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 139
BglI GCCNNNNNGGC 1 cut(s) 893
BisI GCNGC 2 cut(s) 421, 710
BlsI GCNGC 2 cut(s) 422, 711
Bme1390I CCNGG 1 cut(s) 640
Bme18I GGWCC 1 cut(s) 662
BmgT120I GGNCC 3 cut(s) 495, 533, 662
BmiI GGNNCC 3 cut(s) 442, 664, 769
BmrFI CCNGG 1 cut(s) 640
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 2 cut(s) 568, 987
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 3 cut(s) 374, 805, 1009
BpuEI CTTGAG 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 639, 771
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 911
Bse1I ACTGG 3 cut(s) 269, 295, 761
Bse3DI GCAATG 4 cut(s) 201, 303, 885, 960
BseBI CCWGG 1 cut(s) 640
BseDI CCNNGG 2 cut(s) 639, 771
BseGI GGATG 3 cut(s) 497, 523, 583
BseLI CCNNNNNNNGG 2 cut(s) 503, 911
BseMI GCAATG 4 cut(s) 201, 303, 885, 960
BseNI ACTGG 3 cut(s) 269, 295, 761
BseRI GAGGAG 1 cut(s) 780
BseXI GCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 9, 356
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 6 cut(s) 102, 256, 363, 496, 534, 887
BsiEI CGRYCG 1 cut(s) 550
BslFI GGGAC 5 cut(s) 374, 452, 648, 767, 949
BslI CCNNNNNNNGG 2 cut(s) 503, 911
BsmAI GTCTC 1 cut(s) 783
BsmFI GGGAC 5 cut(s) 374, 452, 648, 767, 949
BsmI GAATGC 2 cut(s) 49, 685
BsnI GGCC 6 cut(s) 102, 256, 363, 496, 534, 887
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 2 cut(s) 109, 421
BspANI GGCC 6 cut(s) 102, 256, 363, 496, 534, 887
BspLI GGNNCC 3 cut(s) 442, 664, 769
BspQI GCTCTTC 1 cut(s) 276
BsrDI GCAATG 4 cut(s) 201, 303, 885, 960
BsrI ACTGG 3 cut(s) 269, 295, 761
BssECI CCNNGG 2 cut(s) 639, 771
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 3 cut(s) 128, 358, 781
Bst6I CTCTTC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 514
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 3 cut(s) 497, 523, 583
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 783
BstMBI GATC 1 cut(s) 547
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 5 cut(s) 108, 420, 513, 884, 893
BstNI CCWGG 1 cut(s) 640
BstPI GGTNACC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 638
BstV1I GCAGC 1 cut(s) 721
BstV2I GAAGAC 3 cut(s) 374, 805, 1009
BstXI CCANNNNNNTGG 2 cut(s) 264, 530
BsuRI GGCC 6 cut(s) 102, 256, 363, 496, 534, 887
BtgZI GCGATG 1 cut(s) 401
BtsCI GGATG 3 cut(s) 497, 523, 583
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 2 cut(s) 112, 124
Cac8I GCNNGC 1 cut(s) 514
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 3 cut(s) 495, 533, 662
Csp6I GTAC 1 cut(s) 1024
CviAII CATG 5 cut(s) 252, 311, 326, 836, 862
CviQI GTAC 1 cut(s) 1024
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
EaeI YGGCCR 2 cut(s) 100, 254
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 662
Eco47III AGCGCT 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 771
FaeI CATG 5 cut(s) 255, 314, 329, 839, 865
FaqI GGGAC 5 cut(s) 374, 452, 648, 767, 949
FatI CATG 5 cut(s) 251, 310, 325, 835, 861
FauNDI CATATG 1 cut(s) 529
Fnu4HI GCNGC 2 cut(s) 421, 710
FokI GGATG 3 cut(s) 504, 510, 590
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 421, 710
GlaI GCGC 1 cut(s) 137
GluI GCNGC 2 cut(s) 421, 710
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 6 cut(s) 102, 256, 363, 496, 534, 887
HhaI GCGC 1 cut(s) 138
Hin1II CATG 5 cut(s) 255, 314, 329, 839, 865
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HinfI GANTC 3 cut(s) 740, 798, 937
HphI GGTGA 3 cut(s) 314, 688, 836
Hpy166II GTNNAC 3 cut(s) 148, 554, 784
Hpy188I TCNGA 3 cut(s) 547, 577, 748
Hpy188III TCNNGA 3 cut(s) 229, 551, 802
Hpy8I GTNNAC 3 cut(s) 148, 554, 784
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 732
HpyCH4III ACNGT 3 cut(s) 128, 358, 781
HpyCH4IV ACGT 1 cut(s) 556
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 420, 513, 884, 893
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 5 cut(s) 255, 314, 329, 839, 865
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 1 cut(s) 547
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 143, 767
Lsp1109I GCAGC 1 cut(s) 721
LweI GCATC 2 cut(s) 568, 987
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 1 cut(s) 320
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 5 cut(s) 27, 263, 374, 805, 1009
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 256
MluCI AATT 3 cut(s) 180, 211, 334
MluNI TGGCCA 1 cut(s) 256
MlyI GAGTC 1 cut(s) 931
MnlI CCTC 9 cut(s) 96, 132, 196, 236, 246, 653, 758, 877, 954
Mox20I TGGCCA 1 cut(s) 256
MscI TGGCCA 1 cut(s) 256
MseI TTAA 2 cut(s) 333, 695
MslI CAYNNNNRTG 4 cut(s) 313, 315, 528, 748
Msp20I TGGCCA 1 cut(s) 256
MspR9I CCNGG 1 cut(s) 640
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 2 cut(s) 49, 685
MvaI CCWGG 1 cut(s) 640
MwoI GCNNNNNNNGC 5 cut(s) 108, 420, 513, 884, 893
NdeI CATATG 1 cut(s) 529
NdeII GATC 1 cut(s) 547
NlaIII CATG 5 cut(s) 255, 314, 329, 839, 865
NlaIV GGNNCC 3 cut(s) 442, 664, 769
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 320
OliI CACNNNNGTG 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 276
PctI GAATGC 2 cut(s) 49, 685
PfeI GAWTC 2 cut(s) 740, 798
PkrI GCNGC 2 cut(s) 422, 711
Ple19I CGATCG 1 cut(s) 550
PleI GAGTC 1 cut(s) 931
PpsI GAGTC 1 cut(s) 931
PpuMI RGGWCCY 1 cut(s) 662
Psp1406I AACGTT 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 1 cut(s) 638
PspEI GGTNACC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 3 cut(s) 442, 664, 769
PspPI GGNCC 3 cut(s) 495, 533, 662
PspPPI RGGWCCY 1 cut(s) 662
PsrI GAACNNNNNNTAC 2 cut(s) 911, 943
PstNI CAGNNNCTG 1 cut(s) 53
PvuI CGATCG 1 cut(s) 550
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
RseI CAYNNNNRTG 4 cut(s) 313, 315, 528, 748
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 333, 695
SatI GCNGC 2 cut(s) 421, 710
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 3 cut(s) 495, 533, 662
SchI GAGTC 1 cut(s) 931
ScrFI CCNGG 1 cut(s) 640
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 2 cut(s) 568, 987
SinI GGWCC 1 cut(s) 662
SmiMI CAYNNNNRTG 4 cut(s) 313, 315, 528, 748
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 3 cut(s) 180, 211, 334
SsiI CCGC 2 cut(s) 109, 421
StyD4I CCNGG 1 cut(s) 638
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 3 cut(s) 128, 358, 781
TaiI ACGT 1 cut(s) 559
TaqI TCGA 3 cut(s) 230, 348, 930
TasI AATT 3 cut(s) 180, 211, 334
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 740, 798
Tru1I TTAA 2 cut(s) 333, 695
Tru9I TTAA 2 cut(s) 333, 695
TscAI CASTG 2 cut(s) 119, 131
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 1 cut(s) 709
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 3 cut(s) 471, 732, 1011
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 2 cut(s) 119, 131
VpaK11BI GGWCC 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.