Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002963

Physical Location & Seq

Genomic Coordinates Forward (+)

25102930 .. 25107349

Gene Structure 4420 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023544

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGATTTTCTTTAAGACCTCTTTGCTAGATTTTTGGTCCCTCGCAAGGCCAGTTGCCTCTATTGTTGTGTTGCACGCAAAGCAAGGTAGCAATTACACCTCGACCGGCACCATGGCAGTCGGTTACCTTGTCCTTCTCGCTCCCGTTCCCGTTCCCGTTCCCGTTCCAACCAGGGGCATTCTCGTCCATAAACAAAAATCAGAATGCCCATGTCCGGGTCCCGCCCACAGTACCACGCACGTGATCCCCACAGATATTTTGTCACTAGCTCAGGGCCTGGCGCTTCATAACATAGCCACAGCCCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGCGCCGGAGAGCTTCTCTTTCAGGTTTAGGAACAATGGGTGGTGGTGGAAGAATGTCTGCACCCCCAACTCGCAAGAATGCAGAAACTGACAACATCAAGCGAGTGCCACACCTGAAACCTCCGTTCACTCTCGGCCAAGTCAAGAAAGCCATCCCATCTCACTGTTTCCAGCGCTCCGTCGTCCACTCTTTCTCCTATGTCGTCTATGACCTCGCAATTGCCTCCATCTTTTATTACATTGCTTCAAATTACATCAACCACCTCTCGACACCTCTCTCATTCTTGGCATGGCCAATTTACTGGGCTCTTCAGGGCTGTGTCCTAACTGGTGTCTGGGTCATTGCACACGAGTGTGGTCACCATGCCTTTAGCGATTATCAATGGCTCGATGACACAGTTGGCCTAATCCTGCACTCCTGTCTTCTCGTTCCCTACTTCTCTTGGAAGTATAGCCATCGCCGCCACCATTCTAACACAGGTTCCCTTGAGCGAGATGAAGTCTTTGTCCCAAAACAGAAGTCTGCTATGGGATGGTACTCCAAATACCTTAACAACCCACCAGGCAGAGTCCTTACTCTAACTTTCACACTCACTCTTGGCTGGCCTTTGTATTTGGCATTTAATGTTTCTGGAAGGCCCTATGACGGCTTTGCTTGCCACTACCATCCATATGGCCCTATCTACTCCGATCGTGAGCGTTTGCAGATATACATTTCGGATGCTGGTGTTCTTGCAGTCTGTTATGGGCTTTACCGTCTTGCTGTTGCAAAAGGGCTTGCCTGGGTCTTGTGCTTCTATGGAGGTCCCTTGTTGGTGGTGAATGCGTTTTTGGTTTTAATCACATATTTGCAGCACACCCACCCTTCATTGCCACACTATGATTCATCCGAATGGGACTGGTTGAGAGGAGCCTTGGCAACTGTTGACAGAGACTATGGAATCTTGAACAAGGTCTTCCATAACATCACAGACACTCATGTCGCTCACCATTTGTTCTCAACCATGCCACATTATCACGCAATGGAGGCCACAAAGGCAATCAAGCCGATATTGGGCGAGTATTATCAGTTCGATGGGACTCCATTTTACAAAGCAATGTGGAGGGAAGCAAAGGAGTGCATCTATGTTGAGCCAGATGAAGTTGACAAGAAAGGTGTCTTCTGGTACAAGAATAAGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045485 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko01040 ko01212 map01040 map01212

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

506

Amino Acids

57.19

Weight (kDa)

9.11

Isoelectric Point (pI)

44.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	148 - 185	4e-07	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	208 - 468	1.7e-30	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12120 AT3G12120
fragaria_vesca	FvH4_3g44270 FvH4_6g22400
malus_domestica	MD03G1015100.v1.1 MD12G1057600.v1.1 MD14G1058300.v1.1
prunus_persica	Prupe.7G076500_v2.0.a1 Prupe.7G076500_v2.0.a1
pyrus_communis	pycom03g01260 pycom12g05120 pycom14g04710
rosa_chinensis	RchiOBHm_Chr3g0479761 RchiOBHm_Chr5g0080581
rosa_laevigata	RLG00000023544 RLG00000036873
rosa_multiflora	Rmu_sc0002380.1_g000002 Rmu_sc0003755.1_g000012
rosa_roxburghii	Rroxscaffold_1G00002780 Rroxscaffold_6G00402030
rosa_rugosa	Rorug03G0178000 Rorug03G0178000 Rorug03G0178000 Rorug03G0178100 Rorug03G0178200 Rorug05G0470600
rosa_samantha	Rh3AG228900 Rh3BG262000 Rh3CG258900 Rh3DG255000 Rh5AG524000 Rh5BG546200 Rh5CG569400 Rh5DG556400
rosa_wichuraiana	Rw0G015310 Rw3G020450 Rw3G020630 Rw5G048610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1313
AccB1I	GGYRCC	1 cut(s)	107
AciI	CCGC	2 cut(s)	222, 796
AclWI	GGATC	1 cut(s)	238
AcoI	YGGCCR	2 cut(s)	469, 626
AcuI	CTGAAG	1 cut(s)	629
AcvI	CACGTG	1 cut(s)	241
AfaI	GTAC	3 cut(s)	232, 872, 1502
AfeI	AGCGCT	1 cut(s)	509
AfiI	CCNNNNNNNGG	6 cut(s)	45, 173, 214, 215, 980, 1388
AgsI	TTSAA	2 cut(s)	582, 1282
AjnI	CCWGG	4 cut(s)	170, 276, 895, 1115
AjuI	GAANNNNNNNTTGG	2 cut(s)	1148, 1180
AleI	CACNNNNGTG	3 cut(s)	239, 685, 687
AluBI	AGCT	3 cut(s)	269, 347, 1513
AluI	AGCT	3 cut(s)	269, 347, 1513
Alw26I	GTCTC	1 cut(s)	1260
AlwI	GGATC	1 cut(s)	238
AlwNI	CAGNNNCTG	2 cut(s)	277, 422
Aor51HI	AGCGCT	1 cut(s)	509
AoxI	GGCC	9 cut(s)	47, 274, 469, 626, 736, 938, 971, 1009, 1362
ApeKI	GCWGC	1 cut(s)	1186
ArsI	GACNNNNNNTTYG	2 cut(s)	839, 871
AspLEI	GCGC	3 cut(s)	283, 339, 510
AspS9I	GGNCC	6 cut(s)	36, 218, 274, 972, 1010, 1139
AsuC2I	CCSGG	1 cut(s)	216
AsuHPI	GGTGA	3 cut(s)	686, 1165, 1313
AvaII	GGWCC	3 cut(s)	36, 218, 1139
BalI	TGGCCA	1 cut(s)	628
BanI	GGYRCC	1 cut(s)	107
BanII	GRGCYC	1 cut(s)	643
BauI	CACGAG	1 cut(s)	683
BbrPI	CACGTG	1 cut(s)	241
BbsI	GAAGAC	3 cut(s)	749, 1282, 1486
BbvI	GCAGC	1 cut(s)	1198
BccI	CCATC	7 cut(s)	494, 499, 569, 798, 861, 1008, 1403
BceAI	ACGGC	1 cut(s)	997
BciT130I	CCWGG	4 cut(s)	172, 278, 897, 1117
BcnI	CCSGG	1 cut(s)	216
BcoDI	GTCTC	1 cut(s)	1260
BfaI	CTAG	2 cut(s)	26, 266
BfoI	RGCGCY	3 cut(s)	284, 340, 511
BglI	GCCNNNNNGGC	1 cut(s)	1370
BisI	GCNGC	2 cut(s)	796, 1187
BlsI	GCNGC	2 cut(s)	797, 1188
Bme1390I	CCNGG	5 cut(s)	172, 216, 278, 897, 1117
Bme18I	GGWCC	3 cut(s)	36, 218, 1139
BmgT120I	GGNCC	6 cut(s)	36, 218, 274, 972, 1010, 1139
BmiI	GGNNCC	7 cut(s)	38, 109, 219, 220, 817, 1141, 1246
BmrFI	CCNGG	5 cut(s)	172, 216, 278, 897, 1117
BmrI	ACTGGG	1 cut(s)	646
BmsI	GCATC	2 cut(s)	1045, 1464
BmuI	ACTGGG	1 cut(s)	646
BpiI	GAAGAC	3 cut(s)	749, 1282, 1486
BplI	GAGNNNNNCTC	2 cut(s)	334, 366
Bpu10I	CCTNAGC	1 cut(s)	270
BpuEI	CTTGAG	1 cut(s)	842
BpuMI	CCSGG	1 cut(s)	216
BsaAI	YACGTR	1 cut(s)	241
BsaJI	CCNNGG	4 cut(s)	111, 171, 1116, 1248
BsaXI	ACNNNNNCTCC	2 cut(s)	512, 542
Bsc4I	CCNNNNNNNGG	6 cut(s)	45, 173, 214, 215, 980, 1388
Bse118I	RCCGGY	1 cut(s)	104
Bse1I	ACTGG	4 cut(s)	50, 641, 667, 1238
Bse3DI	GCAATG	5 cut(s)	573, 675, 1202, 1362, 1437
BseBI	CCWGG	4 cut(s)	172, 278, 897, 1117
BseDI	CCNNGG	4 cut(s)	111, 171, 1116, 1248
BseGI	GGATG	5 cut(s)	486, 872, 1000, 1060, 1220
BseLI	CCNNNNNNNGG	6 cut(s)	45, 173, 214, 215, 980, 1388
BseMI	GCAATG	5 cut(s)	573, 675, 1202, 1362, 1437
BseMII	CTCAG	1 cut(s)	284
BseNI	ACTGG	4 cut(s)	50, 641, 667, 1238
BseRI	GAGGAG	1 cut(s)	1257
BseXI	GCAGC	1 cut(s)	1198
BsgI	GTGCAG	2 cut(s)	378, 731
Bsh1285I	CGRYCG	2 cut(s)	105, 1027
BshFI	GGCC	9 cut(s)	49, 276, 471, 628, 738, 940, 973, 1011, 1364
BshNI	GGYRCC	1 cut(s)	107
BsiEI	CGRYCG	2 cut(s)	105, 1027
BsiSI	CCGG	3 cut(s)	105, 215, 340
BslFI	GGGAC	6 cut(s)	22, 204, 827, 1125, 1244, 1426
BslI	CCNNNNNNNGG	6 cut(s)	45, 173, 214, 215, 980, 1388
BsmAI	GTCTC	1 cut(s)	1260
BsmFI	GGGAC	6 cut(s)	22, 204, 827, 1125, 1244, 1426
BsmI	GAATGC	4 cut(s)	177, 209, 418, 1162
BsnI	GGCC	9 cut(s)	49, 276, 471, 628, 738, 940, 973, 1011, 1364
Bsp1286I	GDGCHC	1 cut(s)	643
Bsp143I	GATC	2 cut(s)	243, 1024
Bsp19I	CCATGG	1 cut(s)	111
BspACI	CCGC	2 cut(s)	222, 796
BspANI	GGCC	9 cut(s)	49, 276, 471, 628, 738, 940, 973, 1011, 1364
BspCNI	CTCAG	1 cut(s)	283
BspLI	GGNNCC	7 cut(s)	38, 109, 219, 220, 817, 1141, 1246
BspPI	GGATC	1 cut(s)	238
BspQI	GCTCTTC	1 cut(s)	648
BspT107I	GGYRCC	1 cut(s)	107
BsrDI	GCAATG	5 cut(s)	573, 675, 1202, 1362, 1437
BsrFI	RCCGGY	1 cut(s)	104
BsrI	ACTGG	4 cut(s)	50, 641, 667, 1238
BssAI	RCCGGY	1 cut(s)	104
BssECI	CCNNGG	4 cut(s)	111, 171, 1116, 1248
BssMI	GATC	2 cut(s)	243, 1024
BssSI	CACGAG	1 cut(s)	683
BssT1I	CCWWGG	2 cut(s)	111, 1248
Bst2BI	CACGAG	1 cut(s)	683
Bst2UI	CCWGG	4 cut(s)	172, 278, 897, 1117
Bst4CI	ACNGT	5 cut(s)	230, 500, 733, 1091, 1258
Bst6I	CTCTTC	1 cut(s)	648
BstBAI	YACGTR	1 cut(s)	241
BstC8I	GCNNGC	4 cut(s)	75, 938, 991, 1113
BstDEI	CTNAG	1 cut(s)	270
BstDSI	CCRYGG	1 cut(s)	111
BstEII	GGTNACC	2 cut(s)	122, 692
BstF5I	GGATG	5 cut(s)	486, 872, 1000, 1060, 1220
BstH2I	RGCGCY	3 cut(s)	284, 340, 511
BstHHI	GCGC	3 cut(s)	283, 339, 510
BstKTI	GATC	2 cut(s)	246, 1027
BstMAI	GTCTC	1 cut(s)	1260
BstMBI	GATC	2 cut(s)	243, 1024
BstMCI	CGRYCG	2 cut(s)	105, 1027
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 795, 990, 1361, 1370
BstNI	CCWGG	4 cut(s)	172, 278, 897, 1117
BstPI	GGTNACC	2 cut(s)	122, 692
BstSCI	CCNGG	5 cut(s)	170, 214, 276, 895, 1115
BstV1I	GCAGC	1 cut(s)	1198
BstV2I	GAAGAC	3 cut(s)	749, 1282, 1486
BstXI	CCANNNNNNTGG	2 cut(s)	636, 1007
BsuRI	GGCC	9 cut(s)	49, 276, 471, 628, 738, 940, 973, 1011, 1364
BtgI	CCRYGG	1 cut(s)	111
BtgZI	GCGATG	1 cut(s)	776
BtsCI	GGATG	5 cut(s)	486, 872, 1000, 1060, 1220
BtsIMutI	CAGTG	1 cut(s)	496
Cac8I	GCNNGC	4 cut(s)	75, 938, 991, 1113
CaiI	CAGNNNCTG	2 cut(s)	277, 422
CfoI	GCGC	3 cut(s)	283, 339, 510
Cfr10I	RCCGGY	1 cut(s)	104
Cfr13I	GGNCC	6 cut(s)	36, 218, 274, 972, 1010, 1139
Csp6I	GTAC	3 cut(s)	231, 871, 1501
CviAII	CATG	6 cut(s)	112, 210, 624, 698, 1313, 1339
CviQI	GTAC	3 cut(s)	231, 871, 1501
DdeI	CTNAG	1 cut(s)	270
DpnI	GATC	2 cut(s)	245, 1026
DpnII	GATC	2 cut(s)	243, 1024
DrdI	GACNNNNNNGTC	1 cut(s)	1313
DseDI	GACNNNNNNGTC	1 cut(s)	1313
EaeI	YGGCCR	2 cut(s)	469, 626
Eam1104I	CTCTTC	1 cut(s)	648
EarI	CTCTTC	1 cut(s)	648
Eco130I	CCWWGG	2 cut(s)	111, 1248
Eco24I	GRGCYC	1 cut(s)	643
Eco47I	GGWCC	3 cut(s)	36, 218, 1139
Eco47III	AGCGCT	1 cut(s)	509
Eco57I	CTGAAG	1 cut(s)	629
Eco72I	CACGTG	1 cut(s)	241
Eco91I	GGTNACC	2 cut(s)	122, 692
EcoO109I	RGGNCCY	4 cut(s)	218, 274, 972, 1139
EcoO65I	GGTNACC	2 cut(s)	122, 692
EcoRII	CCWGG	4 cut(s)	170, 276, 895, 1115
EcoT14I	CCWWGG	2 cut(s)	111, 1248
EcoT38I	GRGCYC	1 cut(s)	643
ErhI	CCWWGG	2 cut(s)	111, 1248
FaeI	CATG	6 cut(s)	115, 213, 627, 701, 1316, 1342
FaqI	GGGAC	6 cut(s)	22, 204, 827, 1125, 1244, 1426
FatI	CATG	6 cut(s)	111, 209, 623, 697, 1312, 1338
FauI	CCCGC	1 cut(s)	229
FauNDI	CATATG	1 cut(s)	1006
Fnu4HI	GCNGC	2 cut(s)	796, 1187
FokI	GGATG	5 cut(s)	473, 879, 987, 1067, 1207
FriOI	GRGCYC	1 cut(s)	643
Fsp4HI	GCNGC	2 cut(s)	796, 1187
FspBI	CTAG	2 cut(s)	26, 266
GlaI	GCGC	3 cut(s)	282, 338, 509
GluI	GCNGC	2 cut(s)	796, 1187
HaeII	RGCGCY	3 cut(s)	284, 340, 511
HaeIII	GGCC	9 cut(s)	49, 276, 471, 628, 738, 940, 973, 1011, 1364
HapII	CCGG	3 cut(s)	105, 215, 340
HhaI	GCGC	3 cut(s)	283, 339, 510
Hin1II	CATG	6 cut(s)	115, 213, 627, 701, 1316, 1342
Hin6I	GCGC	3 cut(s)	281, 337, 508
HinP1I	GCGC	3 cut(s)	281, 337, 508
HincII	GTYRAC	2 cut(s)	1261, 1480
HindII	GTYRAC	2 cut(s)	1261, 1480
HinfI	GANTC	4 cut(s)	903, 1217, 1275, 1414
HpaII	CCGG	3 cut(s)	105, 215, 340
HphI	GGTGA	3 cut(s)	686, 1165, 1313
Hpy166II	GTNNAC	4 cut(s)	462, 520, 1261, 1480
Hpy188I	TCNGA	4 cut(s)	202, 1024, 1054, 1225
Hpy188III	TCNNGA	5 cut(s)	478, 601, 966, 1028, 1279
Hpy8I	GTNNAC	4 cut(s)	462, 520, 1261, 1480
Hpy99I	CGWCG	1 cut(s)	518
HpyAV	CCTTC	3 cut(s)	143, 963, 1209
HpyCH4III	ACNGT	5 cut(s)	230, 500, 733, 1091, 1258
HpyCH4IV	ACGT	1 cut(s)	240
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 795, 990, 1361, 1370
HpyF3I	CTNAG	1 cut(s)	270
HpySE526I	ACGT	1 cut(s)	240
Hsp92II	CATG	6 cut(s)	115, 213, 627, 701, 1316, 1342
HspAI	GCGC	3 cut(s)	281, 337, 508
KflI	GGGWCCC	1 cut(s)	218
Kzo9I	GATC	2 cut(s)	243, 1024
LguI	GCTCTTC	1 cut(s)	648
LmnI	GCTCC	3 cut(s)	145, 515, 1244
Lsp1109I	GCAGC	1 cut(s)	1198
LweI	GCATC	2 cut(s)	1045, 1464
MaeI	CTAG	2 cut(s)	26, 266
MaeII	ACGT	1 cut(s)	240
MaeIII	GTNAC	3 cut(s)	122, 261, 692
MalI	GATC	2 cut(s)	245, 1026
MboI	GATC	2 cut(s)	243, 1024
MboII	GAAGA	5 cut(s)	396, 635, 749, 1282, 1486
MfeI	CAATTG	1 cut(s)	552
MhlI	GDGCHC	1 cut(s)	643
MlsI	TGGCCA	1 cut(s)	628
MluCI	AATT	4 cut(s)	91, 552, 583, 630
MluNI	TGGCCA	1 cut(s)	628
MlyI	GAGTC	2 cut(s)	912, 1408
MmeI	TCCRAC	1 cut(s)	191
Mox20I	TGGCCA	1 cut(s)	628
MscI	TGGCCA	1 cut(s)	628
MseI	TTAA	4 cut(s)	12, 885, 957, 1172
MslI	CAYNNNNRTG	5 cut(s)	239, 685, 687, 1005, 1225
Msp20I	TGGCCA	1 cut(s)	628
MspI	CCGG	3 cut(s)	105, 215, 340
MspR9I	CCNGG	5 cut(s)	172, 216, 278, 897, 1117
MunI	CAATTG	1 cut(s)	552
Mva1269I	GAATGC	4 cut(s)	177, 209, 418, 1162
MvaI	CCWGG	4 cut(s)	172, 278, 897, 1117
MwoI	GCNNNNNNNGC	5 cut(s)	79, 795, 990, 1361, 1370
NciI	CCSGG	1 cut(s)	216
NcoI	CCATGG	1 cut(s)	111
NdeI	CATATG	1 cut(s)	1006
NdeII	GATC	2 cut(s)	243, 1024
NlaIII	CATG	6 cut(s)	115, 213, 627, 701, 1316, 1342
NlaIV	GGNNCC	7 cut(s)	38, 109, 219, 220, 817, 1141, 1246
NmeAIII	GCCGAG	1 cut(s)	447
NmuCI	GTSAC	2 cut(s)	261, 692
OliI	CACNNNNGTG	3 cut(s)	239, 685, 687
PciSI	GCTCTTC	1 cut(s)	648
PctI	GAATGC	4 cut(s)	177, 209, 418, 1162
PfeI	GAWTC	2 cut(s)	1217, 1275
PkrI	GCNGC	2 cut(s)	797, 1188
Ple19I	CGATCG	1 cut(s)	1027
PleI	GAGTC	2 cut(s)	911, 1408
PmaCI	CACGTG	1 cut(s)	241
PmlI	CACGTG	1 cut(s)	241
PpsI	GAGTC	2 cut(s)	911, 1408
Ppu21I	YACGTR	1 cut(s)	241
PpuMI	RGGWCCY	2 cut(s)	218, 1139
Psp5II	RGGWCCY	2 cut(s)	218, 1139
Psp6I	CCWGG	4 cut(s)	170, 276, 895, 1115
PspCI	CACGTG	1 cut(s)	241
PspEI	GGTNACC	2 cut(s)	122, 692
PspGI	CCWGG	4 cut(s)	170, 276, 895, 1115
PspN4I	GGNNCC	7 cut(s)	38, 109, 219, 220, 817, 1141, 1246
PspPI	GGNCC	6 cut(s)	36, 218, 274, 972, 1010, 1139
PspPPI	RGGWCCY	2 cut(s)	218, 1139
PsrI	GAACNNNNNNTAC	2 cut(s)	1388, 1420
PstNI	CAGNNNCTG	2 cut(s)	277, 422
PvuI	CGATCG	1 cut(s)	1027
RsaI	GTAC	3 cut(s)	232, 872, 1502
RsaNI	GTAC	3 cut(s)	231, 871, 1501
RseI	CAYNNNNRTG	5 cut(s)	239, 685, 687, 1005, 1225
SapI	GCTCTTC	1 cut(s)	648
SaqAI	TTAA	4 cut(s)	12, 885, 957, 1172
SatI	GCNGC	2 cut(s)	796, 1187
Sau3AI	GATC	2 cut(s)	243, 1024
Sau96I	GGNCC	6 cut(s)	36, 218, 274, 972, 1010, 1139
SchI	GAGTC	2 cut(s)	912, 1408
ScrFI	CCNGG	5 cut(s)	172, 216, 278, 897, 1117
SduI	GDGCHC	1 cut(s)	643
SfaNI	GCATC	2 cut(s)	1045, 1464
SinI	GGWCC	3 cut(s)	36, 218, 1139
SmiMI	CAYNNNNRTG	5 cut(s)	239, 685, 687, 1005, 1225
SmlI	CTYRAG	1 cut(s)	821
SmoI	CTYRAG	1 cut(s)	821
Sse9I	AATT	4 cut(s)	91, 552, 583, 630
SsiI	CCGC	2 cut(s)	222, 796
SspMI	CTAG	2 cut(s)	26, 266
StyD4I	CCNGG	5 cut(s)	170, 214, 276, 895, 1115
StyI	CCWWGG	2 cut(s)	111, 1248
TaaI	ACNGT	5 cut(s)	230, 500, 733, 1091, 1258
TaiI	ACGT	1 cut(s)	243
TaqI	TCGA	4 cut(s)	101, 602, 723, 1407
TasI	AATT	4 cut(s)	91, 552, 583, 630
TauI	GCSGC	1 cut(s)	798
TfiI	GAWTC	2 cut(s)	1217, 1275
Tru1I	TTAA	4 cut(s)	12, 885, 957, 1172
Tru9I	TTAA	4 cut(s)	12, 885, 957, 1172
TscAI	CASTG	1 cut(s)	503
TseFI	GTSAC	2 cut(s)	261, 692
TseI	GCWGC	1 cut(s)	1186
Tsp45I	GTSAC	2 cut(s)	261, 692
TspDTI	ATGAA	5 cut(s)	275, 846, 1191, 1209, 1488
TspGWI	ACGGA	2 cut(s)	447, 502
TspRI	CASTG	1 cut(s)	503
VpaK11BI	GGWCC	3 cut(s)	36, 218, 1139
XspI	CTAG	2 cut(s)	26, 266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.