Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002963

Physical Location & Seq

Genomic Coordinates Reverse (-)

6577929 .. 6581855

Gene Structure 3927 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1057600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGGTTGCGCCCCCTGTGCGCAAAAATGCGGATGCTGACACCCACAAGAGAGTTCCGTACTCTAAGCCTCCGTTCAGCCTCGGTGAGGTCAAGAAAGCCATCCCACCTCATTGTTTTCAGCGCTCTGTTATCCGCTCCTTCTCCTATGTCTTTTATGACCTCACCATTGCCTCTATCCTTTACTACATTGCTTCCACCTACATTCAGAATCTGTCTCAACCTCTATCCTTCTTGGCATGGCCGATTTTCTGGTACGTTCAGGGCTGTGTTCTCACCGGTGTTTGGGTCATAGCACATGAGTGCGGTCACCATGCTTTCAGTGATTATCAATGGCTGGATGACACTGTTGGTTTGATCCTCCACTCTTGCCTCCTTGTCCCGTACTTCTCATGGAAGTATAGCCATCGCCGCCACCATTCCAACACAGGTTCCCTTGAGCGAGATGAAGTCTTTGTCCCCAAGCAGAAGTTCGAAATTGGATGGCATGCCAAATATCTCAACAATCCGCCAGGCAGATTCCTCACACTCCTCATCCAACTCACTCTAGGCTGGCCTTTGTATCTTGCGTTCAATGTTTCTGGAAGGCCCTACAAAGGATTTGCTTGCCACTTTCATCCGTATGGGCCAATCTACTCTGATCGCGAACGATTGCAGATATTTGTGTCCGATGCTGGTGTTCTTGCTGTCTTCTATGGGCTTTACCGTCTTGCCGTTGCAAAGGGACTTGCTTGGGTTATATGCTTCTACGGAGGTCCTCTAATGGTGGTGAATGGATTTTTGGTACTGATCACGTACTTGCAGCACACCCACCCCGCATTGCCGCACTATGATGCCTCTGAATGGGACTGGTTTAGGGGAGCTTTGGCCACCGTTGACAGAGACTACGGAATCCTGAACAAGGTTTTCCACAACATCACAGACACTCACGTTGCGCACCATTTGTTCTCAACCATGCCTCACTATCACGCAATGGAGGCGACCAAGGCAATCAAGCCGATATTGGGCGAGTACTATCAGTTTGACGGGACTCCGGTTTACAAGGCAATGTTTAGAGAGGCGAAGGAGTGTATCTACGTCGAGCCCGATGAGGGTGCCAAGAAAGGTGTCTTCTGGTACAATAAAAAGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016491 GO:0016705 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045485 GO:0055114 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko01040 ko01212 map01040 map01212

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

377

Amino Acids

43.37

Weight (kDa)

8.75

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	18 - 56	5.9e-07	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	79 - 339	3.3e-30	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12120 AT3G12120
fragaria_vesca	FvH4_3g44270 FvH4_6g22400
malus_domestica	MD03G1015100.v1.1 MD12G1057600.v1.1 MD14G1058300.v1.1
prunus_persica	Prupe.7G076500_v2.0.a1 Prupe.7G076500_v2.0.a1
pyrus_communis	pycom03g01260 pycom12g05120 pycom14g04710
rosa_chinensis	RchiOBHm_Chr3g0479761 RchiOBHm_Chr5g0080581
rosa_laevigata	RLG00000023544 RLG00000036873
rosa_multiflora	Rmu_sc0002380.1_g000002 Rmu_sc0003755.1_g000012
rosa_roxburghii	Rroxscaffold_1G00002780 Rroxscaffold_6G00402030
rosa_rugosa	Rorug03G0178000 Rorug03G0178000 Rorug03G0178000 Rorug03G0178100 Rorug03G0178200 Rorug05G0470600
rosa_samantha	Rh3AG228900 Rh3BG262000 Rh3CG258900 Rh3DG255000 Rh5AG524000 Rh5BG546200 Rh5CG569400 Rh5DG556400
rosa_wichuraiana	Rw0G015310 Rw3G020450 Rw3G020630 Rw5G048610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	20, 933
AccB1I	GGYRCC	1 cut(s)	1091
AccBSI	CCGCTC	1 cut(s)	135
AccII	CGCG	1 cut(s)	642
AciI	CCGC	7 cut(s)	29, 133, 303, 409, 506, 813, 821
AclWI	GGATC	1 cut(s)	349
AcoI	YGGCCR	2 cut(s)	239, 864
AfaI	GTAC	7 cut(s)	59, 254, 383, 783, 794, 1010, 1115
AfeI	AGCGCT	1 cut(s)	122
AfiI	CCNNNNNNNGG	7 cut(s)	85, 282, 593, 840, 898, 1001, 1088
AgeI	ACCGGT	1 cut(s)	275
AgsI	TTSAA	1 cut(s)	571
AjnI	CCWGG	1 cut(s)	508
AjuI	GAANNNNNNNTTGG	4 cut(s)	452, 484, 761, 793
AleI	CACNNNNGTG	1 cut(s)	298
AluBI	AGCT	2 cut(s)	860, 1126
AluI	AGCT	2 cut(s)	860, 1126
Alw26I	GTCTC	2 cut(s)	219, 873
AlwI	GGATC	1 cut(s)	349
AlwNI	CAGNNNCTG	1 cut(s)	211
Aor51HI	AGCGCT	1 cut(s)	122
AoxI	GGCC	5 cut(s)	239, 551, 584, 623, 864
ApeKI	GCWGC	1 cut(s)	799
AsiGI	ACCGGT	1 cut(s)	275
AspLEI	GCGC	4 cut(s)	10, 21, 123, 934
AspS9I	GGNCC	3 cut(s)	585, 623, 752
AsuHPI	GGTGA	5 cut(s)	95, 154, 265, 299, 778
AsuII	TTCGAA	1 cut(s)	471
AvaII	GGWCC	1 cut(s)	752
BalI	TGGCCA	1 cut(s)	866
BanI	GGYRCC	1 cut(s)	1091
BanII	GRGCYC	1 cut(s)	1083
BbsI	GAAGAC	2 cut(s)	679, 1099
BbvI	GCAGC	1 cut(s)	811
BccI	CCATC	3 cut(s)	107, 411, 474
BceAI	ACGGC	1 cut(s)	695
BcgI	CGANNNNNNTGC	2 cut(s)	1073, 1107
BciT130I	CCWGG	1 cut(s)	510
BclI	TGATCA	1 cut(s)	786
BcoDI	GTCTC	2 cut(s)	219, 873
BfaI	CTAG	1 cut(s)	545
BfoI	RGCGCY	1 cut(s)	124
BisI	GCNGC	3 cut(s)	409, 800, 821
BlsI	GCNGC	3 cut(s)	410, 801, 822
BmcAI	AGTACT	1 cut(s)	1010
Bme1390I	CCNGG	1 cut(s)	510
Bme18I	GGWCC	1 cut(s)	752
BmgT120I	GGNCC	3 cut(s)	585, 623, 752
BmiI	GGNNCC	2 cut(s)	430, 1093
BmrFI	CCNGG	1 cut(s)	510
BmsI	GCATC	3 cut(s)	22, 658, 820
BpiI	GAAGAC	2 cut(s)	679, 1099
Bpu14I	TTCGAA	1 cut(s)	471
BpuEI	CTTGAG	1 cut(s)	455
BsaAI	YACGTR	1 cut(s)	792
BsaJI	CCNNGG	2 cut(s)	79, 981
BsaWI	WCCGGW	2 cut(s)	275, 1030
Bsc4I	CCNNNNNNNGG	7 cut(s)	85, 282, 593, 840, 898, 1001, 1088
Bse118I	RCCGGY	1 cut(s)	275
Bse1I	ACTGG	1 cut(s)	851
Bse3DI	GCAATG	5 cut(s)	165, 186, 815, 975, 1050
BseBI	CCWGG	1 cut(s)	510
BseDI	CCNNGG	2 cut(s)	79, 981
BseGI	GGATG	6 cut(s)	37, 99, 343, 485, 531, 613
BseLI	CCNNNNNNNGG	7 cut(s)	85, 282, 593, 840, 898, 1001, 1088
BseMI	GCAATG	5 cut(s)	165, 186, 815, 975, 1050
BseNI	ACTGG	1 cut(s)	851
BseRI	GAGGAG	1 cut(s)	518
BseXI	GCAGC	1 cut(s)	811
Bsh1236I	CGCG	1 cut(s)	642
BshFI	GGCC	5 cut(s)	241, 553, 586, 625, 866
BshNI	GGYRCC	1 cut(s)	1091
BshTI	ACCGGT	1 cut(s)	275
BsiSI	CCGG	2 cut(s)	276, 1031
BslFI	GGGAC	5 cut(s)	362, 440, 735, 857, 1039
BslI	CCNNNNNNNGG	7 cut(s)	85, 282, 593, 840, 898, 1001, 1088
BsmAI	GTCTC	2 cut(s)	219, 873
BsmFI	GGGAC	5 cut(s)	362, 440, 735, 857, 1039
BsnI	GGCC	5 cut(s)	241, 553, 586, 625, 866
Bsp119I	TTCGAA	1 cut(s)	471
Bsp1286I	GDGCHC	1 cut(s)	1083
Bsp143I	GATC	3 cut(s)	354, 637, 786
Bsp68I	TCGCGA	1 cut(s)	642
BspACI	CCGC	7 cut(s)	29, 133, 303, 409, 506, 813, 821
BspANI	GGCC	5 cut(s)	241, 553, 586, 625, 866
BspFNI	CGCG	1 cut(s)	642
BspLI	GGNNCC	2 cut(s)	430, 1093
BspPI	GGATC	1 cut(s)	349
BspT104I	TTCGAA	1 cut(s)	471
BspT107I	GGYRCC	1 cut(s)	1091
BsrBI	CCGCTC	1 cut(s)	135
BsrDI	GCAATG	5 cut(s)	165, 186, 815, 975, 1050
BsrFI	RCCGGY	1 cut(s)	275
BsrI	ACTGG	1 cut(s)	851
BssAI	RCCGGY	1 cut(s)	275
BssECI	CCNNGG	2 cut(s)	79, 981
BssMI	GATC	3 cut(s)	354, 637, 786
BssT1I	CCWWGG	1 cut(s)	981
Bst2UI	CCWGG	1 cut(s)	510
Bst4CI	ACNGT	3 cut(s)	346, 704, 871
BstBAI	YACGTR	1 cut(s)	792
BstBI	TTCGAA	1 cut(s)	471
BstC8I	GCNNGC	3 cut(s)	486, 551, 604
BstDEI	CTNAG	1 cut(s)	63
BstEII	GGTNACC	1 cut(s)	305
BstENI	CCTNNNNNAGG	2 cut(s)	83, 896
BstF5I	GGATG	6 cut(s)	37, 99, 343, 485, 531, 613
BstFNI	CGCG	1 cut(s)	642
BstH2I	RGCGCY	1 cut(s)	124
BstHHI	GCGC	4 cut(s)	10, 21, 123, 934
BstKTI	GATC	3 cut(s)	357, 640, 789
BstMAI	GTCTC	2 cut(s)	219, 873
BstMBI	GATC	3 cut(s)	354, 637, 786
BstMWI	GCNNNNNNNGC	4 cut(s)	16, 408, 974, 983
BstNI	CCWGG	1 cut(s)	510
BstNSI	RCATGY	1 cut(s)	488
BstPI	GGTNACC	1 cut(s)	305
BstSCI	CCNGG	1 cut(s)	508
BstUI	CGCG	1 cut(s)	642
BstV1I	GCAGC	1 cut(s)	811
BstV2I	GAAGAC	2 cut(s)	679, 1099
BsuRI	GGCC	5 cut(s)	241, 553, 586, 625, 866
BtgZI	GCGATG	1 cut(s)	389
BtsCI	GGATG	6 cut(s)	37, 99, 343, 485, 531, 613
BtsIMutI	CAGTG	2 cut(s)	325, 342
BtuMI	TCGCGA	1 cut(s)	642
Cac8I	GCNNGC	3 cut(s)	486, 551, 604
CaiI	CAGNNNCTG	1 cut(s)	211
CfoI	GCGC	4 cut(s)	10, 21, 123, 934
Cfr10I	RCCGGY	1 cut(s)	275
Cfr13I	GGNCC	3 cut(s)	585, 623, 752
Csp6I	GTAC	7 cut(s)	58, 253, 382, 782, 793, 1009, 1114
CspAI	ACCGGT	1 cut(s)	275
CviAII	CATG	6 cut(s)	237, 296, 311, 390, 485, 952
CviQI	GTAC	7 cut(s)	58, 253, 382, 782, 793, 1009, 1114
DdeI	CTNAG	1 cut(s)	63
DpnI	GATC	3 cut(s)	356, 639, 788
DpnII	GATC	3 cut(s)	354, 637, 786
EaeI	YGGCCR	2 cut(s)	239, 864
EciI	GGCGGA	1 cut(s)	495
Eco130I	CCWWGG	1 cut(s)	981
Eco24I	GRGCYC	1 cut(s)	1083
Eco47I	GGWCC	1 cut(s)	752
Eco47III	AGCGCT	1 cut(s)	122
Eco91I	GGTNACC	1 cut(s)	305
EcoNI	CCTNNNNNAGG	2 cut(s)	83, 896
EcoO109I	RGGNCCY	2 cut(s)	585, 752
EcoO65I	GGTNACC	1 cut(s)	305
EcoRII	CCWGG	1 cut(s)	508
EcoT14I	CCWWGG	1 cut(s)	981
EcoT38I	GRGCYC	1 cut(s)	1083
ErhI	CCWWGG	1 cut(s)	981
FaeI	CATG	6 cut(s)	240, 299, 314, 393, 488, 955
FaqI	GGGAC	5 cut(s)	362, 440, 735, 857, 1039
FatI	CATG	6 cut(s)	236, 295, 310, 389, 484, 951
FauI	CCCGC	1 cut(s)	820
FbaI	TGATCA	1 cut(s)	786
Fnu4HI	GCNGC	3 cut(s)	409, 800, 821
FokI	GGATG	6 cut(s)	44, 86, 350, 492, 518, 600
FriOI	GRGCYC	1 cut(s)	1083
Fsp4HI	GCNGC	3 cut(s)	409, 800, 821
FspBI	CTAG	1 cut(s)	545
FspI	TGCGCA	2 cut(s)	20, 933
GlaI	GCGC	4 cut(s)	9, 20, 122, 933
GluI	GCNGC	3 cut(s)	409, 800, 821
HaeII	RGCGCY	1 cut(s)	124
HaeIII	GGCC	5 cut(s)	241, 553, 586, 625, 866
HapII	CCGG	2 cut(s)	276, 1031
HhaI	GCGC	4 cut(s)	10, 21, 123, 934
Hin1II	CATG	6 cut(s)	240, 299, 314, 393, 488, 955
Hin6I	GCGC	4 cut(s)	8, 19, 121, 932
HinP1I	GCGC	4 cut(s)	8, 19, 121, 932
HincII	GTYRAC	1 cut(s)	874
HindII	GTYRAC	1 cut(s)	874
HinfI	GANTC	4 cut(s)	208, 516, 888, 1027
HpaII	CCGG	2 cut(s)	276, 1031
HphI	GGTGA	5 cut(s)	95, 154, 265, 299, 778
Hpy166II	GTNNAC	2 cut(s)	874, 1036
Hpy188I	TCNGA	4 cut(s)	207, 637, 667, 838
Hpy188III	TCNNGA	4 cut(s)	91, 579, 641, 892
Hpy8I	GTNNAC	2 cut(s)	874, 1036
Hpy99I	CGWCG	1 cut(s)	1079
HpyAV	CCTTC	4 cut(s)	148, 238, 576, 1054
HpyCH4III	ACNGT	3 cut(s)	346, 704, 871
HpyCH4IV	ACGT	4 cut(s)	255, 791, 927, 1074
HpyCH4V	TGCA	3 cut(s)	652, 716, 799
HpyF10VI	GCNNNNNNNGC	4 cut(s)	16, 408, 974, 983
HpyF3I	CTNAG	1 cut(s)	63
HpySE526I	ACGT	4 cut(s)	255, 791, 927, 1074
Hsp92II	CATG	6 cut(s)	240, 299, 314, 393, 488, 955
HspAI	GCGC	4 cut(s)	8, 19, 121, 932
Ksp22I	TGATCA	1 cut(s)	786
Kzo9I	GATC	3 cut(s)	354, 637, 786
LmnI	GCTCC	2 cut(s)	140, 857
Lsp1109I	GCAGC	1 cut(s)	811
LweI	GCATC	3 cut(s)	22, 658, 820
MaeI	CTAG	1 cut(s)	545
MaeII	ACGT	4 cut(s)	255, 791, 927, 1074
MaeIII	GTNAC	1 cut(s)	305
MalI	GATC	3 cut(s)	356, 639, 788
MbiI	CCGCTC	1 cut(s)	135
MboI	GATC	3 cut(s)	354, 637, 786
MboII	GAAGA	2 cut(s)	679, 1099
MhlI	GDGCHC	1 cut(s)	1083
MlsI	TGGCCA	1 cut(s)	866
MluCI	AATT	1 cut(s)	474
MluNI	TGGCCA	1 cut(s)	866
MlyI	GAGTC	1 cut(s)	1021
MmeI	TCCRAC	2 cut(s)	444, 559
Mox20I	TGGCCA	1 cut(s)	866
MscI	TGGCCA	1 cut(s)	866
MslI	CAYNNNNRTG	3 cut(s)	298, 618, 828
Msp20I	TGGCCA	1 cut(s)	866
MspI	CCGG	2 cut(s)	276, 1031
MspR9I	CCNGG	1 cut(s)	510
MvaI	CCWGG	1 cut(s)	510
MvnI	CGCG	1 cut(s)	642
MwoI	GCNNNNNNNGC	4 cut(s)	16, 408, 974, 983
NdeII	GATC	3 cut(s)	354, 637, 786
NlaIII	CATG	6 cut(s)	240, 299, 314, 393, 488, 955
NlaIV	GGNNCC	2 cut(s)	430, 1093
NmuCI	GTSAC	1 cut(s)	305
NruI	TCGCGA	1 cut(s)	642
NsbI	TGCGCA	2 cut(s)	20, 933
NspI	RCATGY	1 cut(s)	488
NspV	TTCGAA	1 cut(s)	471
OliI	CACNNNNGTG	1 cut(s)	298
PaeI	GCATGC	1 cut(s)	488
PcsI	WCGNNNNNNNCGW	1 cut(s)	1080
PfeI	GAWTC	3 cut(s)	208, 516, 888
PinAI	ACCGGT	1 cut(s)	275
PkrI	GCNGC	3 cut(s)	410, 801, 822
PleI	GAGTC	1 cut(s)	1021
PpsI	GAGTC	1 cut(s)	1021
Ppu21I	YACGTR	1 cut(s)	792
PpuMI	RGGWCCY	1 cut(s)	752
Psp5II	RGGWCCY	1 cut(s)	752
Psp6I	CCWGG	1 cut(s)	508
PspEI	GGTNACC	1 cut(s)	305
PspGI	CCWGG	1 cut(s)	508
PspN4I	GGNNCC	2 cut(s)	430, 1093
PspPI	GGNCC	3 cut(s)	585, 623, 752
PspPPI	RGGWCCY	1 cut(s)	752
PsrI	GAACNNNNNNTAC	2 cut(s)	551, 583
PstNI	CAGNNNCTG	1 cut(s)	211
RruI	TCGCGA	1 cut(s)	642
RsaI	GTAC	7 cut(s)	59, 254, 383, 783, 794, 1010, 1115
RsaNI	GTAC	7 cut(s)	58, 253, 382, 782, 793, 1009, 1114
RseI	CAYNNNNRTG	3 cut(s)	298, 618, 828
SatI	GCNGC	3 cut(s)	409, 800, 821
Sau3AI	GATC	3 cut(s)	354, 637, 786
Sau96I	GGNCC	3 cut(s)	585, 623, 752
ScaI	AGTACT	1 cut(s)	1010
SchI	GAGTC	1 cut(s)	1021
ScrFI	CCNGG	1 cut(s)	510
SduI	GDGCHC	1 cut(s)	1083
SfaNI	GCATC	3 cut(s)	22, 658, 820
SfuI	TTCGAA	1 cut(s)	471
SgrAI	CRCCGGYG	1 cut(s)	275
SinI	GGWCC	1 cut(s)	752
SmiMI	CAYNNNNRTG	3 cut(s)	298, 618, 828
SmlI	CTYRAG	1 cut(s)	434
SmoI	CTYRAG	1 cut(s)	434
SphI	GCATGC	1 cut(s)	488
Sse9I	AATT	1 cut(s)	474
SsiI	CCGC	7 cut(s)	29, 133, 303, 409, 506, 813, 821
SspMI	CTAG	1 cut(s)	545
StyD4I	CCNGG	1 cut(s)	508
StyI	CCWWGG	1 cut(s)	981
TaaI	ACNGT	3 cut(s)	346, 704, 871
TaiI	ACGT	4 cut(s)	258, 794, 930, 1077
TaqI	TCGA	2 cut(s)	471, 1077
TasI	AATT	1 cut(s)	474
TatI	WGTACW	1 cut(s)	1008
TauI	GCSGC	2 cut(s)	411, 823
TfiI	GAWTC	3 cut(s)	208, 516, 888
TscAI	CASTG	2 cut(s)	325, 349
TseFI	GTSAC	1 cut(s)	305
TseI	GCWGC	1 cut(s)	799
Tsp45I	GTSAC	1 cut(s)	305
TspDTI	ATGAA	2 cut(s)	459, 602
TspGWI	ACGGA	5 cut(s)	45, 60, 606, 762, 900
TspRI	CASTG	2 cut(s)	325, 349
VpaK11BI	GGWCC	1 cut(s)	752
XagI	CCTNNNNNAGG	2 cut(s)	83, 896
XceI	RCATGY	1 cut(s)	488
XspI	CTAG	1 cut(s)	545
ZrmI	AGTACT	1 cut(s)	1010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.