MD03G1097200.v1.1

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005413
Physical Location & Seq
Reverse (-)
8357842 .. 8361121
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1097200.v1.1.491

Sequence Viewer

Length: 822 bp
ATGCAGGACCCTCTAAGCATGTCCCTGGACGACCTCATCAAGACCAGCAAGAAATCCGGATCCGGTAACAGCCGAGGTCGCGGCGGCCGGGGGCCCTCGGGACCCGGACCCGCCCGCCGCTTGCCCAATCGCGGCGCCAATCGCGCGGCGCCGTACGTGGCTGCGCCCAAGGCGCCGGAGACTGCGTGGCAACACGACATGTATGGGGATCTTGGGGCGGCGGCGTACGCGGCTCCAGCTGGAAGAGCCTCGGCTATCGAAACTGGAACCAAGCTCTACATCTCCAATCTCGATTATGGCGTTTCCAACGAGGACATTAAGGAATTGTTTTCTGAGGTTGGTGACCTGAAACGTTATGGAGTACATTATGACAGAAGTGGGAGATCAAAGGGAACAGCAGACGTAGTTTTCTCACGACGAACGGATGCTGCGGCGGCTGTTAAAAGATACAACAATGTTCAACTTGATGGGAAGCCGATGAAGATTGAGATTGTAGGAACGAACATTGGGACACCTGGCGCCCCACCTGCTTACCCGCCTGCTCCTAATGTAGCTTTTGGAAATAGGAATGGACCACCCATGGGTGGACAAAGTAGGGGTGGTGCATTTGGACGTATACGTGGCGGTGGTGGCGGCGGTGGTGGCAGTGGTGGTGGCAGTGGTGGTGGCGGTGGTGGCCGCGGTGGCCGTGGTCCTAGAAGGGGCGGTGGTGGACGTGGAAGTGGAAGAGGCCGTGGTGAAAAGGACCAAAAGGTTTCTGCTGAAGATCTTGATGCCGAGCTAGAGAAGTACCATGCAGCAGCAGAATCAATGCAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

274

Amino Acids

27.83

Weight (kDa)

9.91

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 92 - 160 4.2e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 4 cut(s) 134, 148, 172, 518
AccI GTMKAC 1 cut(s) 616
AccII CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
AccIII TCCGGA 1 cut(s) 56
AclI AACGTT 1 cut(s) 352
AclWI GGATC 3 cut(s) 54, 67, 216
AcoI YGGCCR 3 cut(s) 85, 676, 685
AcuI CTGAAG 1 cut(s) 783
AcyI GRCGYC 4 cut(s) 135, 149, 173, 519
AfaI GTAC 4 cut(s) 155, 227, 363, 791
AfiI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 701
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 1 cut(s) 461
AjiI CACGTC 1 cut(s) 716
AjnI CCWGG 2 cut(s) 24, 514
AluBI AGCT 4 cut(s) 239, 274, 554, 781
AluI AGCT 4 cut(s) 239, 274, 554, 781
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 3 cut(s) 54, 67, 216
Ama87I CYCGRG 1 cut(s) 97
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 5 cut(s) 85, 92, 676, 685, 730
ApaI GGGCCC 1 cut(s) 96
ApeKI GCWGC 4 cut(s) 161, 428, 797, 800
AspLEI GCGC 6 cut(s) 137, 146, 151, 166, 175, 521
AspS9I GGNCC 8 cut(s) 7, 92, 93, 101, 107, 572, 692, 745
AsuC2I CCSGG 2 cut(s) 89, 105
AsuHPI GGTGA 2 cut(s) 353, 749
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 6 cut(s) 7, 101, 107, 572, 692, 745
BaeGI GKGCMC 1 cut(s) 96
BamHI GGATCC 1 cut(s) 59
BanI GGYRCC 4 cut(s) 134, 148, 172, 518
BanII GRGCYC 1 cut(s) 96
BbvI GCAGC 4 cut(s) 148, 415, 809, 812
BccI CCATC 1 cut(s) 461
BceAI ACGGC 3 cut(s) 136, 672, 717
BciT130I CCWGG 2 cut(s) 26, 516
BcnI CCSGG 2 cut(s) 89, 105
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 696, 782
BfoI RGCGCY 4 cut(s) 138, 152, 176, 522
BfuAI ACCTGC 1 cut(s) 535
BglI GCCNNNNNGGC 1 cut(s) 684
BglII AGATCT 1 cut(s) 766
Bme1390I CCNGG 4 cut(s) 26, 89, 105, 516
Bme18I GGWCC 6 cut(s) 7, 101, 107, 572, 692, 745
BmeT110I CYCGRG 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 716
BmgT120I GGNCC 8 cut(s) 7, 92, 93, 101, 107, 572, 692, 745
BmrFI CCNGG 4 cut(s) 26, 89, 105, 516
BmsI GCATC 2 cut(s) 415, 763
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 2 cut(s) 89, 105
BsaAI YACGTR 2 cut(s) 157, 620
BsaHI GRCGYC 4 cut(s) 135, 149, 173, 519
BsaWI WCCGGW 2 cut(s) 56, 62
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 581, 584, 701
Bse1I ACTGG 1 cut(s) 268
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 2 cut(s) 26, 516
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 701
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 96
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 4 cut(s) 148, 415, 809, 812
Bsh1236I CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 87, 94, 678, 687, 732
BshNI GGYRCC 4 cut(s) 134, 148, 172, 518
BsiEI CGRYCG 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 5 cut(s) 57, 63, 88, 105, 176
BsiWI CGTACG 2 cut(s) 153, 225
BslFI GGGAC 3 cut(s) 7, 114, 523
BslI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 701
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 3 cut(s) 7, 114, 523
BsnI GGCC 5 cut(s) 87, 94, 678, 687, 732
BsoBI CYCGRG 1 cut(s) 97
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 4 cut(s) 59, 208, 383, 766
Bsp19I CCATGG 1 cut(s) 579
BspANI GGCC 5 cut(s) 87, 94, 678, 687, 732
BspCNI CTCAG 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 3 cut(s) 54, 67, 216
BspQI GCTCTTC 1 cut(s) 238
BspT107I GGYRCC 4 cut(s) 134, 148, 172, 518
BsrI ACTGG 1 cut(s) 268
BssMI GATC 4 cut(s) 59, 208, 383, 766
BssNAI GTATAC 1 cut(s) 617
BssNI GRCGYC 4 cut(s) 135, 149, 173, 519
BssT1I CCWWGG 2 cut(s) 168, 579
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 26, 516
Bst6I CTCTTC 2 cut(s) 238, 721
BstACI GRCGYC 4 cut(s) 135, 149, 173, 519
BstBAI YACGTR 2 cut(s) 157, 620
BstC8I GCNNGC 3 cut(s) 115, 122, 540
BstDEI CTNAG 2 cut(s) 14, 333
BstDSI CCRYGG 4 cut(s) 579, 679, 688, 733
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
BstH2I RGCGCY 4 cut(s) 138, 152, 176, 522
BstHHI GCGC 6 cut(s) 137, 146, 151, 166, 175, 521
BstKTI GATC 4 cut(s) 62, 211, 386, 769
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 59, 208, 383, 766
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 2 cut(s) 26, 516
BstNSI RCATGY 2 cut(s) 22, 202
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 4 cut(s) 24, 87, 103, 514
BstSLI GKGCMC 1 cut(s) 96
BstUI CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
BstV1I GCAGC 4 cut(s) 148, 415, 809, 812
BstX2I RGATCY 3 cut(s) 59, 208, 766
BstYI RGATCY 3 cut(s) 59, 208, 766
BstZ17I GTATAC 1 cut(s) 617
BstZI CGGCCG 1 cut(s) 85
BsuRI GGCC 5 cut(s) 87, 94, 678, 687, 732
BtgI CCRYGG 4 cut(s) 579, 679, 688, 733
BtrI CACGTC 1 cut(s) 716
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 2 cut(s) 652, 664
BtsIMutI CAGTG 2 cut(s) 652, 664
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 3 cut(s) 115, 122, 540
CfoI GCGC 6 cut(s) 137, 146, 151, 166, 175, 521
Cfr13I GGNCC 8 cut(s) 7, 92, 93, 101, 107, 572, 692, 745
Cfr42I CCGCGG 1 cut(s) 682
Csp6I GTAC 4 cut(s) 154, 226, 362, 790
CviAII CATG 4 cut(s) 19, 199, 580, 794
CviQI GTAC 4 cut(s) 154, 226, 362, 790
DdeI CTNAG 2 cut(s) 14, 333
DinI GGCGCC 4 cut(s) 136, 150, 174, 520
DpnI GATC 4 cut(s) 61, 210, 385, 768
DpnII GATC 4 cut(s) 59, 208, 383, 766
EaeI YGGCCR 3 cut(s) 85, 676, 685
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 238, 721
EarI CTCTTC 2 cut(s) 238, 721
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 168, 579
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 6 cut(s) 7, 101, 107, 572, 692, 745
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 783
Eco88I CYCGRG 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 341
EcoO109I RGGNCCY 4 cut(s) 7, 92, 93, 101
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 24, 514
EcoT14I CCWWGG 2 cut(s) 168, 579
EcoT38I GRGCYC 1 cut(s) 96
EgeI GGCGCC 4 cut(s) 136, 150, 174, 520
EheI GGCGCC 4 cut(s) 136, 150, 174, 520
ErhI CCWWGG 2 cut(s) 168, 579
FaeI CATG 4 cut(s) 22, 202, 583, 797
FaiI YATR 9 cut(s) 20, 200, 204, 297, 357, 369, 581, 617, 795
FaqI GGGAC 3 cut(s) 7, 114, 523
FatI CATG 4 cut(s) 18, 198, 579, 793
FauI CCCGC 3 cut(s) 118, 122, 543
FblI GTMKAC 1 cut(s) 616
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 96
FspBI CTAG 2 cut(s) 696, 782
GlaI GCGC 6 cut(s) 136, 145, 150, 165, 174, 520
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 4 cut(s) 138, 152, 176, 522
HaeIII GGCC 5 cut(s) 87, 94, 678, 687, 732
HapII CCGG 5 cut(s) 57, 63, 88, 105, 176
HhaI GCGC 6 cut(s) 137, 146, 151, 166, 175, 521
Hin1I GRCGYC 4 cut(s) 135, 149, 173, 519
Hin1II CATG 4 cut(s) 22, 202, 583, 797
Hin6I GCGC 6 cut(s) 135, 144, 149, 164, 173, 519
HinP1I GCGC 6 cut(s) 135, 144, 149, 164, 173, 519
HinfI GANTC 1 cut(s) 806
HpaII CCGG 5 cut(s) 57, 63, 88, 105, 176
HphI GGTGA 2 cut(s) 353, 749
Hpy166II GTNNAC 3 cut(s) 587, 617, 713
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 6 cut(s) 40, 57, 99, 290, 414, 770
Hpy8I GTNNAC 3 cut(s) 587, 617, 713
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 1 cut(s) 693
HpyCH4IV ACGT 6 cut(s) 156, 352, 402, 613, 619, 715
HpyCH4V TGCA 4 cut(s) 4, 605, 797, 814
HpyF3I CTNAG 2 cut(s) 14, 333
HpySE526I ACGT 6 cut(s) 156, 352, 402, 613, 619, 715
Hsp92I GRCGYC 4 cut(s) 135, 149, 173, 519
Hsp92II CATG 4 cut(s) 22, 202, 583, 797
HspAI GCGC 6 cut(s) 135, 144, 149, 164, 173, 519
KasI GGCGCC 4 cut(s) 134, 148, 172, 518
KflI GGGWCCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 56
KspI CCGCGG 1 cut(s) 682
Kzo9I GATC 4 cut(s) 59, 208, 383, 766
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 238, 547
Lsp1109I GCAGC 4 cut(s) 148, 415, 809, 812
LweI GCATC 2 cut(s) 415, 763
MaeI CTAG 2 cut(s) 696, 782
MaeII ACGT 6 cut(s) 156, 352, 402, 613, 619, 715
MaeIII GTNAC 2 cut(s) 65, 341
MalI GATC 4 cut(s) 61, 210, 385, 768
MboI GATC 4 cut(s) 59, 208, 383, 766
MboII GAAGA 4 cut(s) 255, 493, 738, 776
MflI RGATCY 3 cut(s) 59, 208, 766
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 1 cut(s) 323
Mly113I GGCGCC 4 cut(s) 135, 149, 173, 519
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 8 cut(s) 21, 44, 68, 106, 259, 304, 328, 722
MroI TCCGGA 1 cut(s) 56
MseI TTAA 2 cut(s) 318, 441
MspA1I CMGCKG 2 cut(s) 239, 681
MspI CCGG 5 cut(s) 57, 63, 88, 105, 176
MspR9I CCNGG 4 cut(s) 26, 89, 105, 516
MteI GCGCNGCGC 1 cut(s) 147
MvaI CCWGG 2 cut(s) 26, 516
MvnI CGCG 6 cut(s) 81, 132, 144, 146, 230, 681
NarI GGCGCC 4 cut(s) 135, 149, 173, 519
NciI CCSGG 2 cut(s) 89, 105
NcoI CCATGG 1 cut(s) 579
NdeII GATC 4 cut(s) 59, 208, 383, 766
NlaIII CATG 4 cut(s) 22, 202, 583, 797
NmeAIII GCCGAG 3 cut(s) 98, 230, 802
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 2 cut(s) 22, 202
PaqCI CACCTGC 1 cut(s) 535
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 1 cut(s) 806
Pfl23II CGTACG 2 cut(s) 153, 225
PluTI GGCGCC 4 cut(s) 138, 152, 176, 522
Ppu21I YACGTR 2 cut(s) 157, 620
PpuMI RGGWCCY 2 cut(s) 7, 101
PscI ACATGT 1 cut(s) 198
Psp1406I AACGTT 1 cut(s) 352
Psp5II RGGWCCY 2 cut(s) 7, 101
Psp6I CCWGG 2 cut(s) 24, 514
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 24, 514
PspLI CGTACG 2 cut(s) 153, 225
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 8 cut(s) 7, 92, 93, 101, 107, 572, 692, 745
PspPPI RGGWCCY 2 cut(s) 7, 101
PsuI RGATCY 3 cut(s) 59, 208, 766
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 4 cut(s) 155, 227, 363, 791
RsaNI GTAC 4 cut(s) 154, 226, 362, 790
SacII CCGCGG 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 318, 441
Sau3AI GATC 4 cut(s) 59, 208, 383, 766
Sau96I GGNCC 8 cut(s) 7, 92, 93, 101, 107, 572, 692, 745
ScrFI CCNGG 4 cut(s) 26, 89, 105, 516
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 2 cut(s) 415, 763
SfiI GGCCNNNNNGGCC 1 cut(s) 684
SfoI GGCGCC 4 cut(s) 136, 150, 174, 520
Sfr303I CCGCGG 1 cut(s) 682
SgrBI CCGCGG 1 cut(s) 682
SinI GGWCC 6 cut(s) 7, 101, 107, 572, 692, 745
Sse9I AATT 1 cut(s) 323
SspDI GGCGCC 4 cut(s) 134, 148, 172, 518
SspMI CTAG 2 cut(s) 696, 782
StyD4I CCNGG 4 cut(s) 24, 87, 103, 514
StyI CCWWGG 2 cut(s) 168, 579
TaiI ACGT 6 cut(s) 159, 355, 405, 616, 622, 718
TaqI TCGA 2 cut(s) 258, 291
TasI AATT 1 cut(s) 323
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 2 cut(s) 318, 441
Tru9I TTAA 2 cut(s) 318, 441
TscAI CASTG 2 cut(s) 652, 664
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 4 cut(s) 161, 428, 797, 800
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 652, 664
VpaK11BI GGWCC 6 cut(s) 7, 101, 107, 572, 692, 745
XceI RCATGY 2 cut(s) 22, 202
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 2 cut(s) 696, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.