Rh5AG417000

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005413
Physical Location & Seq
Forward (+)
71922052 .. 71924539
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG417000.1

Sequence Viewer

Length: 489 bp
ATGGATCATCTAAACATGTCCCTCGACGACATCATCAAGACCTCCAAGAAACCCGGATCCGGCAACAGCCGCGGCCGCGCCCGGGCTGCCTCCGGTCCCGGACCCGCCCGCCGCTTGCCCAATCGCGCCGGAAATCGTGCGACGCCCTATGGCGCCGGCGCCGCCAAGGCGCCGGAGTCCACGTGGCAGCACGACATGTTCTCGGATCAGGGAGCGGCCTACGCAGCCTCGGCTGGCAGAGCCTCCGCCATCGAAACTGGAACCAAGCTCTACATCTCTAATCTCGACTATGGCGTTTCCAACGAGGACATCAAGGGTAGAGGTGGCGCATTTGGACGCCCTCGTGGTGGGGGTGGGGGTGGCCGTGGTCCTAGAAGGGGCCGTGGTGGACGTGGAAGAGGAGGGCGTAGTGGTGAAAAGGTTTCTGCTGAAGATCTTGATGCTGAGCTGGAGAAGTACCATGCAACTGCAGAAACAATGCAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

162

Amino Acids

16.8

Weight (kDa)

10.04

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FoP_duplication PF13865 105 - 155 9.3e-10 C-terminal duplication domain of Friend of PRMT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 152, 158, 169
AccBSI CCGCTC 1 cut(s) 215
AccII CGCG 3 cut(s) 72, 78, 126
AciI CCGC 9 cut(s) 70, 72, 76, 105, 109, 112, 162, 215, 246
AclWI GGATC 4 cut(s) 12, 51, 64, 213
AcoI YGGCCR 2 cut(s) 73, 361
AcuI CTGAAG 1 cut(s) 450
AcvI CACGTG 1 cut(s) 183
AcyI GRCGYC 5 cut(s) 143, 153, 159, 170, 337
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 4 cut(s) 59, 82, 347, 377
AflIII ACRYGT 2 cut(s) 15, 195
AjiI CACGTC 1 cut(s) 392
AluBI AGCT 2 cut(s) 268, 448
AluI AGCT 2 cut(s) 268, 448
AlwI GGATC 4 cut(s) 12, 51, 64, 213
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 4 cut(s) 73, 216, 361, 379
ApeKI GCWGC 3 cut(s) 86, 187, 224
AspLEI GCGC 6 cut(s) 80, 128, 155, 161, 172, 329
AspS9I GGNCC 4 cut(s) 95, 101, 368, 379
AsuC2I CCSGG 4 cut(s) 54, 82, 83, 99
AsuHPI GGTGA 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 3 cut(s) 95, 101, 368
BamHI GGATCC 1 cut(s) 56
BanI GGYRCC 3 cut(s) 152, 158, 169
BauI CACGAG 1 cut(s) 342
BbrPI CACGTG 1 cut(s) 183
BbvI GCAGC 3 cut(s) 73, 199, 236
BccI CCATC 1 cut(s) 257
BceAI ACGGC 2 cut(s) 348, 366
BcnI CCSGG 4 cut(s) 54, 82, 83, 99
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 3 cut(s) 156, 162, 173
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 433
BisI GCNGC 9 cut(s) 70, 73, 76, 87, 112, 162, 188, 216, 225
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 9 cut(s) 71, 74, 77, 88, 113, 163, 189, 217, 226
Bme1390I CCNGG 4 cut(s) 54, 82, 83, 99
Bme18I GGWCC 3 cut(s) 95, 101, 368
BmeT110I CYCGRG 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 4 cut(s) 95, 101, 368, 379
BmiI GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 380
BmrFI CCNGG 4 cut(s) 54, 82, 83, 99
BmsI GCATC 1 cut(s) 430
BpmI CTGGAG 1 cut(s) 470
Bpu1102I GCTNAGC 1 cut(s) 444
BpuMI CCSGG 4 cut(s) 54, 82, 83, 99
BsaAI YACGTR 1 cut(s) 183
BsaHI GRCGYC 5 cut(s) 143, 153, 159, 170, 337
BsaJI CCNNGG 6 cut(s) 70, 81, 165, 228, 364, 382
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 82, 347, 377
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 262
BseDI CCNNGG 6 cut(s) 70, 81, 165, 228, 364, 382
BseLI CCNNNNNNNGG 4 cut(s) 59, 82, 347, 377
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 414
BseX3I CGGCCG 1 cut(s) 73
BseXI GCAGC 3 cut(s) 73, 199, 236
Bsh1236I CGCG 3 cut(s) 72, 78, 126
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 4 cut(s) 75, 218, 363, 381
BshNI GGYRCC 3 cut(s) 152, 158, 169
BsiEI CGRYCG 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
BslFI GGGAC 2 cut(s) 4, 81
BslI CCNNNNNNNGG 4 cut(s) 59, 82, 347, 377
BsmFI GGGAC 2 cut(s) 4, 81
BsnI GGCC 4 cut(s) 75, 218, 363, 381
BsoBI CYCGRG 1 cut(s) 81
Bsp143I GATC 4 cut(s) 4, 56, 205, 433
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 9 cut(s) 70, 72, 76, 105, 109, 112, 162, 215, 246
BspANI GGCC 4 cut(s) 75, 218, 363, 381
BspCNI CTCAG 1 cut(s) 436
BspFNI CGCG 3 cut(s) 72, 78, 126
BspLI GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 380
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 4 cut(s) 12, 51, 64, 213
BspT107I GGYRCC 3 cut(s) 152, 158, 169
BsrBI CCGCTC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 6 cut(s) 70, 81, 165, 228, 364, 382
BssMI GATC 4 cut(s) 4, 56, 205, 433
BssNI GRCGYC 5 cut(s) 143, 153, 159, 170, 337
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 342
Bst6I CTCTTC 1 cut(s) 391
BstACI GRCGYC 5 cut(s) 143, 153, 159, 170, 337
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 4 cut(s) 109, 116, 157, 235
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 3 cut(s) 70, 364, 382
BstFNI CGCG 3 cut(s) 72, 78, 126
BstH2I RGCGCY 3 cut(s) 156, 162, 173
BstHHI GCGC 6 cut(s) 80, 128, 155, 161, 172, 329
BstKTI GATC 4 cut(s) 7, 59, 208, 436
BstMBI GATC 4 cut(s) 4, 56, 205, 433
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 9 cut(s) 69, 75, 86, 161, 167, 221, 224, 230, 239
BstNSI RCATGY 2 cut(s) 19, 199
BstSCI CCNGG 4 cut(s) 52, 80, 81, 97
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 3 cut(s) 72, 78, 126
BstV1I GCAGC 3 cut(s) 73, 199, 236
BstX2I RGATCY 2 cut(s) 56, 433
BstYI RGATCY 2 cut(s) 56, 433
BstZI CGGCCG 1 cut(s) 73
BsuRI GGCC 4 cut(s) 75, 218, 363, 381
BtgI CCRYGG 3 cut(s) 70, 364, 382
BtrI CACGTC 1 cut(s) 392
Cac8I GCNNGC 4 cut(s) 109, 116, 157, 235
CciNI GCGGCCGC 1 cut(s) 73
CfoI GCGC 6 cut(s) 80, 128, 155, 161, 172, 329
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 95, 101, 368, 379
Cfr42I CCGCGG 1 cut(s) 73
Cfr9I CCCGGG 1 cut(s) 81
CseI GACGC 2 cut(s) 151, 345
Csp6I GTAC 1 cut(s) 457
CviAII CATG 3 cut(s) 16, 196, 461
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 444
DinI GGCGCC 3 cut(s) 154, 160, 171
DpnI GATC 4 cut(s) 6, 58, 207, 435
DpnII GATC 4 cut(s) 4, 56, 205, 433
EaeI YGGCCR 2 cut(s) 73, 361
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
EciI GGCGGA 1 cut(s) 235
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 165
Eco47I GGWCC 3 cut(s) 95, 101, 368
Eco52I CGGCCG 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 450
Eco72I CACGTG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 165
EgeI GGCGCC 3 cut(s) 154, 160, 171
EheI GGCGCC 3 cut(s) 154, 160, 171
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 3 cut(s) 19, 199, 464
FaiI YATR 5 cut(s) 17, 150, 197, 291, 462
FaqI GGGAC 2 cut(s) 4, 81
FatI CATG 3 cut(s) 15, 195, 460
FauI CCCGC 2 cut(s) 112, 116
Fnu4HI GCNGC 9 cut(s) 70, 73, 76, 87, 112, 162, 188, 216, 225
Fsp4HI GCNGC 9 cut(s) 70, 73, 76, 87, 112, 162, 188, 216, 225
FspBI CTAG 1 cut(s) 372
GlaI GCGC 6 cut(s) 79, 127, 154, 160, 171, 328
GluI GCNGC 9 cut(s) 70, 73, 76, 87, 112, 162, 188, 216, 225
GsuI CTGGAG 1 cut(s) 470
HaeII RGCGCY 3 cut(s) 156, 162, 173
HaeIII GGCC 4 cut(s) 75, 218, 363, 381
HapII CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
HgaI GACGC 2 cut(s) 151, 345
HhaI GCGC 6 cut(s) 80, 128, 155, 161, 172, 329
Hin1I GRCGYC 5 cut(s) 143, 153, 159, 170, 337
Hin1II CATG 3 cut(s) 19, 199, 464
Hin6I GCGC 6 cut(s) 78, 126, 153, 159, 170, 327
HinP1I GCGC 6 cut(s) 78, 126, 153, 159, 170, 327
HinfI GANTC 1 cut(s) 176
HpaII CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 180, 389
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 3 cut(s) 37, 284, 437
Hpy8I GTNNAC 2 cut(s) 180, 389
Hpy99I CGWCG 2 cut(s) 29, 145
HpyAV CCTTC 1 cut(s) 369
HpyCH4IV ACGT 2 cut(s) 182, 391
HpyCH4V TGCA 3 cut(s) 464, 470, 481
HpyF10VI GCNNNNNNNGC 9 cut(s) 69, 75, 86, 161, 167, 221, 224, 230, 239
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 2 cut(s) 182, 391
Hsp92I GRCGYC 5 cut(s) 143, 153, 159, 170, 337
Hsp92II CATG 3 cut(s) 19, 199, 464
HspAI GCGC 6 cut(s) 78, 126, 153, 159, 170, 327
KasI GGCGCC 3 cut(s) 152, 158, 169
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 4, 56, 205, 433
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 3 cut(s) 73, 199, 236
LweI GCATC 1 cut(s) 430
MaeI CTAG 1 cut(s) 372
MaeII ACGT 2 cut(s) 182, 391
MalI GATC 4 cut(s) 6, 58, 207, 435
MbiI CCGCTC 1 cut(s) 215
MboI GATC 4 cut(s) 4, 56, 205, 433
MboII GAAGA 2 cut(s) 408, 443
MflI RGATCY 2 cut(s) 56, 433
Mly113I GGCGCC 3 cut(s) 153, 159, 170
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 324
MreI CGCCGGCG 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
MspR9I CCNGG 4 cut(s) 54, 82, 83, 99
MvnI CGCG 3 cut(s) 72, 78, 126
MwoI GCNNNNNNNGC 9 cut(s) 69, 75, 86, 161, 167, 221, 224, 230, 239
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 3 cut(s) 153, 159, 170
NciI CCSGG 4 cut(s) 54, 82, 83, 99
NdeII GATC 4 cut(s) 4, 56, 205, 433
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 3 cut(s) 19, 199, 464
NlaIV GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 380
NmeAIII GCCGAG 1 cut(s) 209
NotI GCGGCCGC 1 cut(s) 73
NspI RCATGY 2 cut(s) 19, 199
PciI ACATGT 2 cut(s) 15, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 9 cut(s) 71, 74, 77, 88, 113, 163, 189, 217, 226
PleI GAGTC 1 cut(s) 184
PluTI GGCGCC 3 cut(s) 156, 162, 173
PmaCI CACGTG 1 cut(s) 183
PmlI CACGTG 1 cut(s) 183
PpsI GAGTC 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 2 cut(s) 15, 195
PspCI CACGTG 1 cut(s) 183
PspN4I GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 380
PspPI GGNCC 4 cut(s) 95, 101, 368, 379
PstI CTGCAG 1 cut(s) 472
PsuI RGATCY 2 cut(s) 56, 433
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SacII CCGCGG 1 cut(s) 73
SatI GCNGC 9 cut(s) 70, 73, 76, 87, 112, 162, 188, 216, 225
Sau3AI GATC 4 cut(s) 4, 56, 205, 433
Sau96I GGNCC 4 cut(s) 95, 101, 368, 379
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 4 cut(s) 54, 82, 83, 99
SetI ASST 7 cut(s) 44, 185, 270, 325, 394, 423, 450
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 468
SfoI GGCGCC 3 cut(s) 154, 160, 171
Sfr303I CCGCGG 1 cut(s) 73
SgrAI CRCCGGYG 1 cut(s) 155
SgrBI CCGCGG 1 cut(s) 73
SinI GGWCC 3 cut(s) 95, 101, 368
SmaI CCCGGG 1 cut(s) 83
SrfI GCCCGGGC 1 cut(s) 83
SsiI CCGC 9 cut(s) 70, 72, 76, 105, 109, 112, 162, 215, 246
SspDI GGCGCC 3 cut(s) 152, 158, 169
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 4 cut(s) 52, 80, 81, 97
StyI CCWWGG 1 cut(s) 165
TaiI ACGT 2 cut(s) 185, 394
TaqI TCGA 3 cut(s) 24, 252, 285
TauI GCSGC 6 cut(s) 72, 75, 78, 114, 164, 218
TseI GCWGC 3 cut(s) 86, 187, 224
TspMI CCCGGG 1 cut(s) 81
VpaK11BI GGWCC 3 cut(s) 95, 101, 368
XceI RCATGY 2 cut(s) 19, 199
XmaI CCCGGG 1 cut(s) 81
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.