MD11G1143800.v1.1

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005413
Physical Location & Seq
Reverse (-)
13378709 .. 13381888
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1143800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGCAGGACCCTCTAAGCATGTCCCTGGACGACCTCATCAAGACCAGCAAGAAATCGGGATCCGGGAACGCCCGAGGCCGCGGCGGCCGGGGGACCTCCGGACCCGGACCCGCCCGCCGCTTGCCGAATCGCGGCGCCAATCGCGCGACGCCGTACGTGGCTGCGGCCAAGGCGCCGGAGACTGCGTGGCAACACGACATGTATGGGGATCTTGGTGCGGTGGCGTACGCGGCTCCAGCTGGGAGAGCATCGGCTATCGAAACCGGAACCAAGCTCTACATCTCCAATCTCGATTATGGCGTTTCCAACGAGGACATTAAGGAATTGTTTTCTGAGGTTGGTGACCTGAAACGTTATGGAGTACATTATGACAGAAGCGGGAGATCAAAGGGAACGGCAGACGTAGTTTTCTCACGACGAACGGATGCTGCGGCGGCTGTTAAAAGATACAACAATGTTCAGCTTGATGGGAAGCCGATGAAGATTGAGATTGTAGGAACAAACATTGGGACACCTGGAGCCCCACCTGCTTTCCCCCCTGCTGCTAATGGAAATTTTGGAAATCGGAATGGACTACCCATGGGGGGACAGAGTAGGGGTGGTGCATTTGGACGTATACGTGGCGGCGGTGGTGGTGGCGGAGGTGGTGGTGGCGGCAGCCGTGGTGGCCGTGGTCCTAGAAGGGGCGGTGGTGGACGCGGAAGTGGAAGAGGCCGTGGTGAAAAGGACCAAAAGGTTTCCGCTGAAGATCTTGATGCCGAGCTAGAGAAGTACCATGCAGCAGCAGAATCAATGCAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

268

Amino Acids

27.45

Weight (kDa)

9.95

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 92 - 160 4.1e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 2 cut(s) 134, 172
AccI GTMKAC 1 cut(s) 616
AccII CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
AccIII TCCGGA 1 cut(s) 98
AclI AACGTT 1 cut(s) 352
AclWI GGATC 3 cut(s) 54, 67, 216
AcoI YGGCCR 3 cut(s) 85, 165, 667
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 3 cut(s) 135, 149, 173
AfaI GTAC 4 cut(s) 155, 227, 363, 773
AfiI CCNNNNNNNGG 3 cut(s) 131, 584, 683
AflIII ACRYGT 1 cut(s) 198
AjnI CCWGG 2 cut(s) 24, 514
AluBI AGCT 4 cut(s) 239, 274, 463, 763
AluI AGCT 4 cut(s) 239, 274, 463, 763
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 3 cut(s) 54, 67, 216
Ama87I CYCGRG 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 98
AoxI GGCC 5 cut(s) 76, 85, 165, 667, 712
ApeKI GCWGC 6 cut(s) 161, 428, 542, 657, 779, 782
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 3 cut(s) 137, 146, 175
AspS9I GGNCC 6 cut(s) 7, 93, 101, 107, 674, 727
AsuC2I CCSGG 3 cut(s) 64, 89, 105
AsuHPI GGTGA 2 cut(s) 353, 731
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 6 cut(s) 7, 93, 101, 107, 674, 727
BamHI GGATCC 1 cut(s) 59
BanI GGYRCC 2 cut(s) 134, 172
BanII GRGCYC 1 cut(s) 523
BbvI GCAGC 6 cut(s) 148, 415, 529, 669, 791, 794
BccI CCATC 1 cut(s) 461
BceAI ACGGC 5 cut(s) 136, 411, 645, 654, 699
BciT130I CCWGG 2 cut(s) 26, 516
BcnI CCSGG 3 cut(s) 64, 89, 105
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 678, 764
BfoI RGCGCY 2 cut(s) 138, 176
BfuAI ACCTGC 1 cut(s) 535
BglI GCCNNNNNGGC 2 cut(s) 84, 666
BglII AGATCT 1 cut(s) 748
Bme1390I CCNGG 5 cut(s) 26, 64, 89, 105, 516
Bme18I GGWCC 6 cut(s) 7, 93, 101, 107, 674, 727
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 6 cut(s) 7, 93, 101, 107, 674, 727
BmrFI CCNGG 5 cut(s) 26, 64, 89, 105, 516
BmsI GCATC 3 cut(s) 257, 415, 745
BpmI CTGGAG 2 cut(s) 219, 537
BpuMI CCSGG 3 cut(s) 64, 89, 105
BsaAI YACGTR 2 cut(s) 157, 620
BsaHI GRCGYC 3 cut(s) 135, 149, 173
BsaJI CCNNGG 9 cut(s) 24, 73, 79, 88, 168, 579, 661, 670, 715
BsaWI WCCGGW 2 cut(s) 98, 263
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 584, 683
BseAI TCCGGA 1 cut(s) 98
BseBI CCWGG 2 cut(s) 26, 516
BseDI CCNNGG 9 cut(s) 24, 73, 79, 88, 168, 579, 661, 670, 715
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 3 cut(s) 131, 584, 683
BseMII CTCAG 1 cut(s) 324
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 6 cut(s) 148, 415, 529, 669, 791, 794
BseYI CCCAGC 1 cut(s) 239
Bsh1236I CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 78, 87, 167, 669, 714
BshNI GGYRCC 2 cut(s) 134, 172
BsiEI CGRYCG 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 6 cut(s) 63, 88, 99, 105, 176, 264
BsiWI CGTACG 2 cut(s) 153, 225
BslFI GGGAC 4 cut(s) 7, 106, 523, 600
BslI CCNNNNNNNGG 3 cut(s) 131, 584, 683
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 4 cut(s) 7, 106, 523, 600
BsnI GGCC 5 cut(s) 78, 87, 167, 669, 714
BsoBI CYCGRG 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 523
Bsp13I TCCGGA 1 cut(s) 98
Bsp143I GATC 4 cut(s) 59, 208, 383, 748
Bsp19I CCATGG 1 cut(s) 579
BspANI GGCC 5 cut(s) 78, 87, 167, 669, 714
BspCNI CTCAG 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 98
BspFNI CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 3 cut(s) 54, 67, 216
BspT107I GGYRCC 2 cut(s) 134, 172
BssECI CCNNGG 9 cut(s) 24, 73, 79, 88, 168, 579, 661, 670, 715
BssMI GATC 4 cut(s) 59, 208, 383, 748
BssNAI GTATAC 1 cut(s) 617
BssNI GRCGYC 3 cut(s) 135, 149, 173
BssT1I CCWWGG 2 cut(s) 168, 579
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 26, 516
Bst6I CTCTTC 1 cut(s) 703
BstACI GRCGYC 3 cut(s) 135, 149, 173
BstBAI YACGTR 2 cut(s) 157, 620
BstC8I GCNNGC 2 cut(s) 115, 122
BstDEI CTNAG 2 cut(s) 14, 333
BstDSI CCRYGG 5 cut(s) 79, 579, 661, 670, 715
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
BstH2I RGCGCY 2 cut(s) 138, 176
BstHHI GCGC 3 cut(s) 137, 146, 175
BstKTI GATC 4 cut(s) 62, 211, 386, 751
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 59, 208, 383, 748
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 2 cut(s) 26, 516
BstNSI RCATGY 2 cut(s) 22, 202
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 5 cut(s) 24, 62, 87, 103, 514
BstUI CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
BstV1I GCAGC 6 cut(s) 148, 415, 529, 669, 791, 794
BstX2I RGATCY 3 cut(s) 59, 208, 748
BstYI RGATCY 3 cut(s) 59, 208, 748
BstZ17I GTATAC 1 cut(s) 617
BstZI CGGCCG 1 cut(s) 85
BsuRI GGCC 5 cut(s) 78, 87, 167, 669, 714
BtgI CCRYGG 5 cut(s) 79, 579, 661, 670, 715
BtsCI GGATG 1 cut(s) 430
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 2 cut(s) 115, 122
CfoI GCGC 3 cut(s) 137, 146, 175
Cfr13I GGNCC 6 cut(s) 7, 93, 101, 107, 674, 727
Cfr42I CCGCGG 1 cut(s) 82
CseI GACGC 2 cut(s) 157, 705
Csp6I GTAC 4 cut(s) 154, 226, 362, 772
CviAII CATG 4 cut(s) 19, 199, 580, 776
CviQI GTAC 4 cut(s) 154, 226, 362, 772
DdeI CTNAG 2 cut(s) 14, 333
DinI GGCGCC 2 cut(s) 136, 174
DpnI GATC 4 cut(s) 61, 210, 385, 750
DpnII GATC 4 cut(s) 59, 208, 383, 748
EaeI YGGCCR 3 cut(s) 85, 165, 667
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 703
EciI GGCGGA 1 cut(s) 654
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 168, 579
Eco24I GRGCYC 1 cut(s) 523
Eco47I GGWCC 6 cut(s) 7, 93, 101, 107, 674, 727
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 765
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 7, 93
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 24, 514
EcoT14I CCWWGG 2 cut(s) 168, 579
EcoT38I GRGCYC 1 cut(s) 523
EgeI GGCGCC 2 cut(s) 136, 174
EheI GGCGCC 2 cut(s) 136, 174
ErhI CCWWGG 2 cut(s) 168, 579
FaeI CATG 4 cut(s) 22, 202, 583, 779
FaiI YATR 9 cut(s) 20, 200, 204, 297, 357, 369, 581, 617, 777
FaqI GGGAC 4 cut(s) 7, 106, 523, 600
FatI CATG 4 cut(s) 18, 198, 579, 775
FauI CCCGC 3 cut(s) 118, 122, 371
FblI GTMKAC 1 cut(s) 616
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 523
FspBI CTAG 2 cut(s) 678, 764
GlaI GCGC 3 cut(s) 136, 145, 174
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 2 cut(s) 219, 537
HaeII RGCGCY 2 cut(s) 138, 176
HaeIII GGCC 5 cut(s) 78, 87, 167, 669, 714
HapII CCGG 6 cut(s) 63, 88, 99, 105, 176, 264
HgaI GACGC 2 cut(s) 157, 705
HhaI GCGC 3 cut(s) 137, 146, 175
Hin1I GRCGYC 3 cut(s) 135, 149, 173
Hin1II CATG 4 cut(s) 22, 202, 583, 779
Hin6I GCGC 3 cut(s) 135, 144, 173
HinP1I GCGC 3 cut(s) 135, 144, 173
HinfI GANTC 2 cut(s) 127, 788
HpaII CCGG 6 cut(s) 63, 88, 99, 105, 176, 264
HphI GGTGA 2 cut(s) 353, 731
Hpy166II GTNNAC 2 cut(s) 617, 695
Hpy188I TCNGA 2 cut(s) 334, 567
Hpy188III TCNNGA 6 cut(s) 40, 57, 99, 290, 414, 752
Hpy8I GTNNAC 2 cut(s) 617, 695
Hpy99I CGWCG 2 cut(s) 151, 420
HpyAV CCTTC 1 cut(s) 675
HpyCH4IV ACGT 5 cut(s) 156, 352, 402, 613, 619
HpyCH4V TGCA 4 cut(s) 4, 605, 779, 796
HpyF3I CTNAG 2 cut(s) 14, 333
HpySE526I ACGT 5 cut(s) 156, 352, 402, 613, 619
Hsp92I GRCGYC 3 cut(s) 135, 149, 173
Hsp92II CATG 4 cut(s) 22, 202, 583, 779
HspAI GCGC 3 cut(s) 135, 144, 173
KasI GGCGCC 2 cut(s) 134, 172
Kpn2I TCCGGA 1 cut(s) 98
KspI CCGCGG 1 cut(s) 82
Kzo9I GATC 4 cut(s) 59, 208, 383, 748
LmnI GCTCC 2 cut(s) 238, 518
Lsp1109I GCAGC 6 cut(s) 148, 415, 529, 669, 791, 794
LweI GCATC 3 cut(s) 257, 415, 745
MaeI CTAG 2 cut(s) 678, 764
MaeII ACGT 5 cut(s) 156, 352, 402, 613, 619
MaeIII GTNAC 1 cut(s) 341
MalI GATC 4 cut(s) 61, 210, 385, 750
MboI GATC 4 cut(s) 59, 208, 383, 748
MboII GAAGA 3 cut(s) 493, 720, 758
MflI RGATCY 3 cut(s) 59, 208, 748
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 2 cut(s) 323, 553
Mly113I GGCGCC 2 cut(s) 135, 173
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 8 cut(s) 21, 44, 68, 106, 304, 328, 635, 704
MroI TCCGGA 1 cut(s) 98
MseI TTAA 2 cut(s) 318, 441
MspA1I CMGCKG 3 cut(s) 81, 239, 743
MspI CCGG 6 cut(s) 63, 88, 99, 105, 176, 264
MspR9I CCNGG 5 cut(s) 26, 64, 89, 105, 516
MvaI CCWGG 2 cut(s) 26, 516
MvnI CGCG 6 cut(s) 81, 132, 144, 146, 230, 699
NarI GGCGCC 2 cut(s) 135, 173
NciI CCSGG 3 cut(s) 64, 89, 105
NcoI CCATGG 1 cut(s) 579
NdeII GATC 4 cut(s) 59, 208, 383, 748
NlaIII CATG 4 cut(s) 22, 202, 583, 779
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 2 cut(s) 22, 202
PaqCI CACCTGC 1 cut(s) 535
PciI ACATGT 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 2 cut(s) 127, 788
Pfl23II CGTACG 2 cut(s) 153, 225
PfoI TCCNGGA 1 cut(s) 62
PluTI GGCGCC 2 cut(s) 138, 176
Ppu21I YACGTR 2 cut(s) 157, 620
PpuMI RGGWCCY 2 cut(s) 7, 93
PscI ACATGT 1 cut(s) 198
Psp1406I AACGTT 1 cut(s) 352
Psp5II RGGWCCY 2 cut(s) 7, 93
Psp6I CCWGG 2 cut(s) 24, 514
PspEI GGTNACC 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 2 cut(s) 24, 514
PspLI CGTACG 2 cut(s) 153, 225
PspPI GGNCC 6 cut(s) 7, 93, 101, 107, 674, 727
PspPPI RGGWCCY 2 cut(s) 7, 93
PsuI RGATCY 3 cut(s) 59, 208, 748
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 4 cut(s) 155, 227, 363, 773
RsaNI GTAC 4 cut(s) 154, 226, 362, 772
SacII CCGCGG 1 cut(s) 82
SaqAI TTAA 2 cut(s) 318, 441
Sau3AI GATC 4 cut(s) 59, 208, 383, 748
Sau96I GGNCC 6 cut(s) 7, 93, 101, 107, 674, 727
ScrFI CCNGG 5 cut(s) 26, 64, 89, 105, 516
SduI GDGCHC 1 cut(s) 523
SfaNI GCATC 3 cut(s) 257, 415, 745
SfiI GGCCNNNNNGGCC 1 cut(s) 84
SfoI GGCGCC 2 cut(s) 136, 174
Sfr303I CCGCGG 1 cut(s) 82
SgrBI CCGCGG 1 cut(s) 82
SinI GGWCC 6 cut(s) 7, 93, 101, 107, 674, 727
Sse9I AATT 2 cut(s) 323, 553
SspDI GGCGCC 2 cut(s) 134, 172
SspMI CTAG 2 cut(s) 678, 764
StyD4I CCNGG 5 cut(s) 24, 62, 87, 103, 514
StyI CCWWGG 2 cut(s) 168, 579
TaiI ACGT 5 cut(s) 159, 355, 405, 616, 622
TaqI TCGA 2 cut(s) 258, 291
TasI AATT 2 cut(s) 323, 553
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 2 cut(s) 127, 788
Tru1I TTAA 2 cut(s) 318, 441
Tru9I TTAA 2 cut(s) 318, 441
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 6 cut(s) 161, 428, 542, 657, 779, 782
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 437
VpaK11BI GGWCC 6 cut(s) 7, 93, 101, 107, 674, 727
XapI RAATTY 1 cut(s) 553
XceI RCATGY 2 cut(s) 22, 202
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 2 cut(s) 678, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.