pycom03g08020

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005413
Physical Location & Seq
Reverse (-)
6414505 .. 6416617
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08020.5

Sequence Viewer

Length: 825 bp
ATGCAGGACCCTCTAAGCATGTCCCTGGACGACCTCATCAAGACCAGCAAGAAATCCGGATCCGGTAACACCCGAGGTCGCGGCGGCCGGGGGCCTTCCGGACCCGGACCAGCCCGCCGCTTGCCCAATCGAGGCGCCAATCGCGCGGCACCGTACGTGGCTGCGGCCAAGGCGCCGGAGACTGCGTGGCAACACGACATGTATGGGGATCTTGGGGCTGCGGCGTACGCGGCTCCAGCTGGAAGAGCCTCGGCTATCGAAACCGGAACCAAGCTCTACATCTCCAATCTCGATTATGGCGTTTCCAACGAGGACATTAAGGAATTGTTTTCTGAGGTTGGTGACCTGAAACGTTATGGAGTACATTATGACAGAAGTGGGAGATCAAAGGGAACGGCAGACGTAGTTTTCTCACGACGAACGGATGCTGCGGCGGCTGTTAAAAGATACAACAATGTTCAACTTGATGGGAAGCCGATGAAGATTGAGATTGTAGGAACGAACATTGGGACACCTGGCGCCCCACCTGCGTTCCTGCCTGCTCCTAATTTAGCTTTTGGAAATCGGAATGGACCACCCATGGGTGGACAAAGTAGGGGTGGTGCATTTGGACGTATACGTGGCGGCGGTGGCGGCAGTGGTGGTGGCGGTGGCGGTGGCGGCGATGGTGGCAGTGGTGGCCGCGGTGGCCGTGGTCCTAGAAGGGGCCGTGGTGGACGTGGAAGTGGAAGAGGCCGTGGTGAAAAGGACCAAAAGGTTTCTGCTGAAGATCTTGATGCCGAGCTAGAGAAGTACCACGCAGCAGCAGAATCAATGCAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

275

Amino Acids

28.04

Weight (kDa)

9.95

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 4 cut(s) 134, 148, 172, 518
AccI GTMKAC 1 cut(s) 616
AccII CGCG 5 cut(s) 81, 144, 146, 230, 684
AccIII TCCGGA 2 cut(s) 56, 98
AclI AACGTT 1 cut(s) 352
AclWI GGATC 3 cut(s) 54, 67, 216
AcoI YGGCCR 4 cut(s) 85, 165, 679, 688
AcuI CTGAAG 1 cut(s) 786
AcyI GRCGYC 3 cut(s) 135, 173, 519
AfaI GTAC 4 cut(s) 155, 227, 363, 794
AfiI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 704
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 1 cut(s) 461
AjiI CACGTC 1 cut(s) 719
AjnI CCWGG 2 cut(s) 24, 514
AluBI AGCT 4 cut(s) 239, 274, 554, 784
AluI AGCT 4 cut(s) 239, 274, 554, 784
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 3 cut(s) 54, 67, 216
Ama87I CYCGRG 1 cut(s) 72
Aor13HI TCCGGA 2 cut(s) 56, 98
AoxI GGCC 7 cut(s) 85, 92, 165, 679, 688, 706, 733
ApeKI GCWGC 5 cut(s) 161, 218, 428, 800, 803
AspLEI GCGC 4 cut(s) 137, 146, 175, 521
AspS9I GGNCC 8 cut(s) 7, 92, 101, 107, 572, 695, 706, 748
AsuC2I CCSGG 2 cut(s) 89, 105
AsuHPI GGTGA 2 cut(s) 353, 752
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 6 cut(s) 7, 101, 107, 572, 695, 748
BamHI GGATCC 1 cut(s) 59
BanI GGYRCC 4 cut(s) 134, 148, 172, 518
BbvI GCAGC 5 cut(s) 148, 205, 415, 812, 815
BccI CCATC 2 cut(s) 461, 659
BceAI ACGGC 4 cut(s) 411, 675, 693, 720
BciT130I CCWGG 2 cut(s) 26, 516
BcnI CCSGG 2 cut(s) 89, 105
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 699, 785
BfoI RGCGCY 3 cut(s) 138, 176, 522
BfuAI ACCTGC 1 cut(s) 535
BglI GCCNNNNNGGC 1 cut(s) 687
BglII AGATCT 1 cut(s) 769
Bme1390I CCNGG 4 cut(s) 26, 89, 105, 516
Bme18I GGWCC 6 cut(s) 7, 101, 107, 572, 695, 748
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 719
BmgT120I GGNCC 8 cut(s) 7, 92, 101, 107, 572, 695, 706, 748
BmrFI CCNGG 4 cut(s) 26, 89, 105, 516
BmsI GCATC 2 cut(s) 415, 766
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 2 cut(s) 89, 105
BsaAI YACGTR 2 cut(s) 157, 620
BsaHI GRCGYC 3 cut(s) 135, 173, 519
BsaWI WCCGGW 4 cut(s) 56, 62, 98, 263
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 581, 584, 704
BseAI TCCGGA 2 cut(s) 56, 98
BseBI CCWGG 2 cut(s) 26, 516
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 704
BseMII CTCAG 1 cut(s) 324
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 5 cut(s) 148, 205, 415, 812, 815
Bsh1236I CGCG 5 cut(s) 81, 144, 146, 230, 684
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 87, 94, 167, 681, 690, 708, 735
BshNI GGYRCC 4 cut(s) 134, 148, 172, 518
BsiEI CGRYCG 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 7 cut(s) 57, 63, 88, 99, 105, 176, 264
BsiWI CGTACG 2 cut(s) 153, 225
BslFI GGGAC 2 cut(s) 7, 523
BslI CCNNNNNNNGG 4 cut(s) 131, 581, 584, 704
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 7, 523
BsnI GGCC 7 cut(s) 87, 94, 167, 681, 690, 708, 735
BsoBI CYCGRG 1 cut(s) 72
Bsp13I TCCGGA 2 cut(s) 56, 98
Bsp143I GATC 4 cut(s) 59, 208, 383, 769
Bsp19I CCATGG 1 cut(s) 579
BspANI GGCC 7 cut(s) 87, 94, 167, 681, 690, 708, 735
BspCNI CTCAG 1 cut(s) 325
BspEI TCCGGA 2 cut(s) 56, 98
BspFNI CGCG 5 cut(s) 81, 144, 146, 230, 684
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 3 cut(s) 54, 67, 216
BspQI GCTCTTC 1 cut(s) 238
BspT107I GGYRCC 4 cut(s) 134, 148, 172, 518
BssMI GATC 4 cut(s) 59, 208, 383, 769
BssNAI GTATAC 1 cut(s) 617
BssNI GRCGYC 3 cut(s) 135, 173, 519
BssT1I CCWWGG 2 cut(s) 168, 579
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 26, 516
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 2 cut(s) 238, 724
BstACI GRCGYC 3 cut(s) 135, 173, 519
BstBAI YACGTR 2 cut(s) 157, 620
BstC8I GCNNGC 3 cut(s) 115, 122, 540
BstDEI CTNAG 2 cut(s) 14, 333
BstDSI CCRYGG 5 cut(s) 579, 682, 691, 709, 736
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 5 cut(s) 81, 144, 146, 230, 684
BstH2I RGCGCY 3 cut(s) 138, 176, 522
BstHHI GCGC 4 cut(s) 137, 146, 175, 521
BstKTI GATC 4 cut(s) 62, 211, 386, 772
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 59, 208, 383, 769
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 2 cut(s) 26, 516
BstNSI RCATGY 2 cut(s) 22, 202
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 4 cut(s) 24, 87, 103, 514
BstUI CGCG 5 cut(s) 81, 144, 146, 230, 684
BstV1I GCAGC 5 cut(s) 148, 205, 415, 812, 815
BstX2I RGATCY 3 cut(s) 59, 208, 769
BstYI RGATCY 3 cut(s) 59, 208, 769
BstZ17I GTATAC 1 cut(s) 617
BstZI CGGCCG 1 cut(s) 85
BsuRI GGCC 7 cut(s) 87, 94, 167, 681, 690, 708, 735
BtgI CCRYGG 5 cut(s) 579, 682, 691, 709, 736
BtgZI GCGATG 1 cut(s) 678
BtrI CACGTC 1 cut(s) 719
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 2 cut(s) 643, 679
BtsIMutI CAGTG 2 cut(s) 643, 679
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 3 cut(s) 115, 122, 540
CfoI GCGC 4 cut(s) 137, 146, 175, 521
Cfr13I GGNCC 8 cut(s) 7, 92, 101, 107, 572, 695, 706, 748
Cfr42I CCGCGG 1 cut(s) 685
Csp6I GTAC 4 cut(s) 154, 226, 362, 793
CviAII CATG 3 cut(s) 19, 199, 580
CviQI GTAC 4 cut(s) 154, 226, 362, 793
DdeI CTNAG 2 cut(s) 14, 333
DinI GGCGCC 3 cut(s) 136, 174, 520
DpnI GATC 4 cut(s) 61, 210, 385, 771
DpnII GATC 4 cut(s) 59, 208, 383, 769
EaeI YGGCCR 4 cut(s) 85, 165, 679, 688
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 238, 724
EarI CTCTTC 2 cut(s) 238, 724
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 168, 579
Eco47I GGWCC 6 cut(s) 7, 101, 107, 572, 695, 748
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 7, 92
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 24, 514
EcoT14I CCWWGG 2 cut(s) 168, 579
EgeI GGCGCC 3 cut(s) 136, 174, 520
EheI GGCGCC 3 cut(s) 136, 174, 520
ErhI CCWWGG 2 cut(s) 168, 579
FaeI CATG 3 cut(s) 22, 202, 583
FaiI YATR 8 cut(s) 20, 200, 204, 297, 357, 369, 581, 617
FaqI GGGAC 2 cut(s) 7, 523
FatI CATG 3 cut(s) 18, 198, 579
FauI CCCGC 1 cut(s) 122
FblI GTMKAC 1 cut(s) 616
FokI GGATG 1 cut(s) 437
FspBI CTAG 2 cut(s) 699, 785
GlaI GCGC 4 cut(s) 136, 145, 174, 520
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 3 cut(s) 138, 176, 522
HaeIII GGCC 7 cut(s) 87, 94, 167, 681, 690, 708, 735
HapII CCGG 7 cut(s) 57, 63, 88, 99, 105, 176, 264
HhaI GCGC 4 cut(s) 137, 146, 175, 521
Hin1I GRCGYC 3 cut(s) 135, 173, 519
Hin1II CATG 3 cut(s) 22, 202, 583
Hin6I GCGC 4 cut(s) 135, 144, 173, 519
HinP1I GCGC 4 cut(s) 135, 144, 173, 519
HinfI GANTC 1 cut(s) 809
HpaII CCGG 7 cut(s) 57, 63, 88, 99, 105, 176, 264
HphI GGTGA 2 cut(s) 353, 752
Hpy166II GTNNAC 3 cut(s) 587, 617, 716
Hpy188I TCNGA 2 cut(s) 334, 567
Hpy188III TCNNGA 6 cut(s) 40, 57, 99, 290, 414, 773
Hpy8I GTNNAC 3 cut(s) 587, 617, 716
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 2 cut(s) 105, 696
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4IV ACGT 6 cut(s) 156, 352, 402, 613, 619, 718
HpyCH4V TGCA 3 cut(s) 4, 605, 817
HpyF3I CTNAG 2 cut(s) 14, 333
HpySE526I ACGT 6 cut(s) 156, 352, 402, 613, 619, 718
Hsp92I GRCGYC 3 cut(s) 135, 173, 519
Hsp92II CATG 3 cut(s) 22, 202, 583
HspAI GCGC 4 cut(s) 135, 144, 173, 519
KasI GGCGCC 3 cut(s) 134, 172, 518
Kpn2I TCCGGA 2 cut(s) 56, 98
KspI CCGCGG 1 cut(s) 685
Kzo9I GATC 4 cut(s) 59, 208, 383, 769
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 238, 547
Lsp1109I GCAGC 5 cut(s) 148, 205, 415, 812, 815
LweI GCATC 2 cut(s) 415, 766
MaeI CTAG 2 cut(s) 699, 785
MaeII ACGT 6 cut(s) 156, 352, 402, 613, 619, 718
MaeIII GTNAC 2 cut(s) 65, 341
MalI GATC 4 cut(s) 61, 210, 385, 771
MboI GATC 4 cut(s) 59, 208, 383, 769
MboII GAAGA 4 cut(s) 255, 493, 741, 779
MflI RGATCY 3 cut(s) 59, 208, 769
MluCI AATT 2 cut(s) 323, 547
Mly113I GGCGCC 3 cut(s) 135, 173, 519
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 8 cut(s) 21, 44, 68, 125, 259, 304, 328, 725
MroI TCCGGA 2 cut(s) 56, 98
MseI TTAA 2 cut(s) 318, 441
MspA1I CMGCKG 2 cut(s) 239, 684
MspI CCGG 7 cut(s) 57, 63, 88, 99, 105, 176, 264
MspR9I CCNGG 4 cut(s) 26, 89, 105, 516
MvaI CCWGG 2 cut(s) 26, 516
MvnI CGCG 5 cut(s) 81, 144, 146, 230, 684
NarI GGCGCC 3 cut(s) 135, 173, 519
NciI CCSGG 2 cut(s) 89, 105
NcoI CCATGG 1 cut(s) 579
NdeII GATC 4 cut(s) 59, 208, 383, 769
NlaIII CATG 3 cut(s) 22, 202, 583
NmeAIII GCCGAG 2 cut(s) 230, 805
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 2 cut(s) 22, 202
PaqCI CACCTGC 1 cut(s) 535
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 1 cut(s) 809
Pfl23II CGTACG 2 cut(s) 153, 225
PluTI GGCGCC 3 cut(s) 138, 176, 522
Ppu21I YACGTR 2 cut(s) 157, 620
PpuMI RGGWCCY 1 cut(s) 7
PscI ACATGT 1 cut(s) 198
Psp1406I AACGTT 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 24, 514
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 24, 514
PspLI CGTACG 2 cut(s) 153, 225
PspPI GGNCC 8 cut(s) 7, 92, 101, 107, 572, 695, 706, 748
PspPPI RGGWCCY 1 cut(s) 7
PsuI RGATCY 3 cut(s) 59, 208, 769
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 4 cut(s) 155, 227, 363, 794
RsaNI GTAC 4 cut(s) 154, 226, 362, 793
SacII CCGCGG 1 cut(s) 685
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 318, 441
Sau3AI GATC 4 cut(s) 59, 208, 383, 769
Sau96I GGNCC 8 cut(s) 7, 92, 101, 107, 572, 695, 706, 748
ScrFI CCNGG 4 cut(s) 26, 89, 105, 516
SfaNI GCATC 2 cut(s) 415, 766
SfiI GGCCNNNNNGGCC 1 cut(s) 687
SfoI GGCGCC 3 cut(s) 136, 174, 520
Sfr303I CCGCGG 1 cut(s) 685
SgrBI CCGCGG 1 cut(s) 685
SinI GGWCC 6 cut(s) 7, 101, 107, 572, 695, 748
Sse9I AATT 2 cut(s) 323, 547
SspDI GGCGCC 3 cut(s) 134, 172, 518
SspMI CTAG 2 cut(s) 699, 785
StyD4I CCNGG 4 cut(s) 24, 87, 103, 514
StyI CCWWGG 2 cut(s) 168, 579
TaaI ACNGT 1 cut(s) 153
TaiI ACGT 6 cut(s) 159, 355, 405, 616, 622, 721
TaqI TCGA 3 cut(s) 130, 258, 291
TasI AATT 2 cut(s) 323, 547
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 809
Tru1I TTAA 2 cut(s) 318, 441
Tru9I TTAA 2 cut(s) 318, 441
TscAI CASTG 2 cut(s) 643, 679
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 5 cut(s) 161, 218, 428, 800, 803
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 643, 679
VpaK11BI GGWCC 6 cut(s) 7, 101, 107, 572, 695, 748
XceI RCATGY 2 cut(s) 22, 202
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 2 cut(s) 699, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.