Prupe.6G081700_v2.0.a1

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0005413
Physical Location & Seq
Reverse (-)
5527416 .. 5531836
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G081700.3

Sequence Viewer

Length: 759 bp
ATGCAGGACCCTCTAAACATGTCCCTCGACGACCTCATCAAGACCAGCAAGAAATCCGGATCGGGCAACGCTCGAGGCCGTGGCCGGGCCTCAGGACCCGGACCCGCCCGCCGGTTGCCCAATCGTGCCGCCAATCGTACGACGCCGTATGCGGCGGCCAAGGCGCCGGAGACCGCGTGGCAACACGACCTGTATACGGATCAGGGGGCGGCGGCGTTCCCGGCTCAAGCTGGAAGAGCCTCGGCCATTGAAACTGGAACCAAGCTCTACATCTCCAATCTCGATTATGGCGTTTCCAACGAGGACATTAAGGAACTGTTTTCAGAGGTTGGTGACCTGAAGCGATATGGAGTACATTATGACCGAAGTGGGAGATCGAAGGGAACGGCAGAAGTAGTCTTCTCACGACGACCAGATGCTGTTGCCGCGGTTAAAAGATACAACAATGTTCAGCTTGATGGGAAACCAATGAAGATAGAGATTGTAGGAACGAACATTTCAACACCTGGCGGACCTCCTACTTTGCCCCCTGCTGCTAATGGCAACTTTGGAAATTCGAATGGAGTTCCTAGGGGTGGACAGAGTAGGGGTGGTGCATTTGGACGTATACGTGGCGGGGGTGGCCGTGGTCCTAGAAGGGGGGGCCGTGGACGTGGAAGTGGTAATGGAGGTGGAAGAGGCCGTGGTGGTGAAAAGGTATCTGCTGAAGATCTTGATGCCGAGCTGGAGAAGTACCATGCAGAAGCAATGCAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

253

Amino Acids

26.42

Weight (kDa)

10.02

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AccI GTMKAC 2 cut(s) 194, 607
AccII CGCG 2 cut(s) 176, 428
AccIII TCCGGA 1 cut(s) 56
AclWI GGATC 2 cut(s) 67, 207
AcoI YGGCCR 4 cut(s) 82, 156, 243, 622
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 2 cut(s) 359, 726
AcyI GRCGYC 2 cut(s) 143, 164
AfaI GTAC 3 cut(s) 139, 354, 734
AfiI CCNNNNNNNGG 5 cut(s) 85, 111, 196, 575, 638
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 2 cut(s) 251, 501
AjiI CACGTC 1 cut(s) 653
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 4 cut(s) 230, 265, 454, 724
AluI AGCT 4 cut(s) 230, 265, 454, 724
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 2 cut(s) 67, 207
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 8 cut(s) 76, 82, 87, 156, 243, 622, 643, 679
ApeKI GCWGC 1 cut(s) 533
ApoI RAATTY 1 cut(s) 553
AspA2I CCTAGG 1 cut(s) 569
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 7 cut(s) 7, 87, 95, 101, 512, 629, 643
AsuC2I CCSGG 3 cut(s) 86, 99, 221
AsuHPI GGTGA 2 cut(s) 344, 701
AsuII TTCGAA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 5 cut(s) 7, 95, 101, 512, 629
AvrII CCTAGG 1 cut(s) 569
AxyI CCTNAGG 1 cut(s) 91
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 391
BbvI GCAGC 1 cut(s) 520
BccI CCATC 1 cut(s) 452
BceAI ACGGC 6 cut(s) 63, 130, 402, 609, 630, 666
BciT130I CCWGG 1 cut(s) 507
BcnI CCSGG 3 cut(s) 86, 99, 221
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 2 cut(s) 570, 633
BfoI RGCGCY 1 cut(s) 167
BglII AGATCT 1 cut(s) 709
BisI GCNGC 7 cut(s) 129, 153, 156, 210, 213, 426, 534
BlnI CCTAGG 1 cut(s) 569
BlsI GCNGC 7 cut(s) 130, 154, 157, 211, 214, 427, 535
Bme1390I CCNGG 4 cut(s) 86, 99, 221, 507
Bme18I GGWCC 5 cut(s) 7, 95, 101, 512, 629
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 653
BmgT120I GGNCC 7 cut(s) 7, 87, 95, 101, 512, 629, 643
BmiI GGNNCC 6 cut(s) 9, 97, 103, 165, 259, 644
BmrFI CCNGG 4 cut(s) 86, 99, 221, 507
BmsI GCATC 2 cut(s) 406, 706
BpiI GAAGAC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 746
Bpu14I TTCGAA 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 210
BpuMI CCSGG 3 cut(s) 86, 99, 221
BsaAI YACGTR 1 cut(s) 611
BsaHI GRCGYC 2 cut(s) 143, 164
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 8 cut(s) 79, 159, 240, 426, 569, 625, 646, 682
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 111, 196, 575, 638
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 259
Bse21I CCTNAGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 8 cut(s) 79, 159, 240, 426, 569, 625, 646, 682
BseLI CCNNNNNNNGG 5 cut(s) 85, 111, 196, 575, 638
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 520
Bsh1236I CGCG 2 cut(s) 176, 428
BshFI GGCC 8 cut(s) 78, 84, 89, 158, 245, 624, 645, 681
BshNI GGYRCC 1 cut(s) 163
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 6 cut(s) 57, 85, 99, 112, 167, 221
BsiWI CGTACG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 7
BslI CCNNNNNNNGG 5 cut(s) 85, 111, 196, 575, 638
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 7
BsnI GGCC 8 cut(s) 78, 84, 89, 158, 245, 624, 645, 681
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 557
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 4 cut(s) 59, 199, 374, 709
BspANI GGCC 8 cut(s) 78, 84, 89, 158, 245, 624, 645, 681
BspCNI CTCAG 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 2 cut(s) 176, 428
BspLI GGNNCC 6 cut(s) 9, 97, 103, 165, 259, 644
BspPI GGATC 2 cut(s) 67, 207
BspQI GCTCTTC 1 cut(s) 229
BspT104I TTCGAA 1 cut(s) 557
BspT107I GGYRCC 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 753
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 8 cut(s) 79, 159, 240, 426, 569, 625, 646, 682
BssMI GATC 4 cut(s) 59, 199, 374, 709
BssNAI GTATAC 2 cut(s) 195, 608
BssNI GRCGYC 2 cut(s) 143, 164
BssT1I CCWWGG 2 cut(s) 159, 569
Bst1107I GTATAC 2 cut(s) 195, 608
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 229, 670
BstACI GRCGYC 2 cut(s) 143, 164
BstBAI YACGTR 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 91
BstDSI CCRYGG 5 cut(s) 79, 426, 625, 646, 682
BstEII GGTNACC 1 cut(s) 332
BstFNI CGCG 2 cut(s) 176, 428
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 4 cut(s) 62, 202, 377, 712
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 4 cut(s) 59, 199, 374, 709
BstMWI GCNNNNNNNGC 5 cut(s) 161, 221, 236, 425, 621
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 22
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 4 cut(s) 84, 97, 219, 505
BstUI CGCG 2 cut(s) 176, 428
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BstZ17I GTATAC 2 cut(s) 195, 608
Bsu36I CCTNAGG 1 cut(s) 91
BsuRI GGCC 8 cut(s) 78, 84, 89, 158, 245, 624, 645, 681
BtgI CCRYGG 5 cut(s) 79, 426, 625, 646, 682
BtrI CACGTC 1 cut(s) 653
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 419
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 7 cut(s) 7, 87, 95, 101, 512, 629, 643
Cfr42I CCGCGG 1 cut(s) 429
CseI GACGC 1 cut(s) 151
Csp6I GTAC 3 cut(s) 138, 353, 733
CviAII CATG 2 cut(s) 19, 737
CviQI GTAC 3 cut(s) 138, 353, 733
DdeI CTNAG 1 cut(s) 91
DinI GGCGCC 1 cut(s) 165
DpnI GATC 4 cut(s) 61, 201, 376, 711
DpnII GATC 4 cut(s) 59, 199, 374, 709
EaeI YGGCCR 4 cut(s) 82, 156, 243, 622
Eam1104I CTCTTC 2 cut(s) 229, 670
EarI CTCTTC 2 cut(s) 229, 670
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 159, 569
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 5 cut(s) 7, 95, 101, 512, 629
Eco57I CTGAAG 2 cut(s) 359, 726
Eco81I CCTNAGG 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 7, 95
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 2 cut(s) 159, 569
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 2 cut(s) 159, 569
FaeI CATG 2 cut(s) 22, 740
FaiI YATR 8 cut(s) 20, 150, 195, 288, 348, 360, 608, 738
FaqI GGGAC 1 cut(s) 7
FatI CATG 2 cut(s) 18, 736
FauI CCCGC 3 cut(s) 112, 116, 608
FblI GTMKAC 2 cut(s) 194, 607
Fnu4HI GCNGC 7 cut(s) 129, 153, 156, 210, 213, 426, 534
Fsp4HI GCNGC 7 cut(s) 129, 153, 156, 210, 213, 426, 534
FspBI CTAG 2 cut(s) 570, 633
GlaI GCGC 1 cut(s) 165
GluI GCNGC 7 cut(s) 129, 153, 156, 210, 213, 426, 534
GsuI CTGGAG 1 cut(s) 746
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 8 cut(s) 78, 84, 89, 158, 245, 624, 645, 681
HapII CCGG 6 cut(s) 57, 85, 99, 112, 167, 221
HgaI GACGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 2 cut(s) 143, 164
Hin1II CATG 2 cut(s) 22, 740
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HpaII CCGG 6 cut(s) 57, 85, 99, 112, 167, 221
HphI GGTGA 2 cut(s) 344, 701
Hpy166II GTNNAC 4 cut(s) 195, 578, 608, 650
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 6 cut(s) 40, 57, 93, 281, 405, 713
Hpy8I GTNNAC 4 cut(s) 195, 578, 608, 650
Hpy99I CGWCG 3 cut(s) 32, 145, 411
HpyAV CCTTC 2 cut(s) 373, 630
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 3 cut(s) 604, 610, 652
HpyCH4V TGCA 4 cut(s) 4, 596, 740, 751
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 221, 236, 425, 621
HpyF3I CTNAG 1 cut(s) 91
HpySE526I ACGT 3 cut(s) 604, 610, 652
Hsp92I GRCGYC 2 cut(s) 143, 164
Hsp92II CATG 2 cut(s) 22, 740
HspAI GCGC 1 cut(s) 164
KasI GGCGCC 1 cut(s) 163
Kpn2I TCCGGA 1 cut(s) 56
KspI CCGCGG 1 cut(s) 429
Kzo9I GATC 4 cut(s) 59, 199, 374, 709
LguI GCTCTTC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 2 cut(s) 406, 706
MaeI CTAG 2 cut(s) 570, 633
MaeII ACGT 3 cut(s) 604, 610, 652
MaeIII GTNAC 1 cut(s) 332
MalI GATC 4 cut(s) 61, 201, 376, 711
MboI GATC 4 cut(s) 59, 199, 374, 709
MboII GAAGA 5 cut(s) 246, 391, 484, 687, 719
MflI RGATCY 1 cut(s) 709
MluCI AATT 1 cut(s) 553
Mly113I GGCGCC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 321
MroI TCCGGA 1 cut(s) 56
MseI TTAA 2 cut(s) 309, 432
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 6 cut(s) 57, 85, 99, 112, 167, 221
MspR9I CCNGG 4 cut(s) 86, 99, 221, 507
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 2 cut(s) 176, 428
MwoI GCNNNNNNNGC 5 cut(s) 161, 221, 236, 425, 621
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 3 cut(s) 86, 99, 221
NdeII GATC 4 cut(s) 59, 199, 374, 709
NlaIII CATG 2 cut(s) 22, 740
NlaIV GGNNCC 6 cut(s) 9, 97, 103, 165, 259, 644
NmeAIII GCCGAG 2 cut(s) 221, 745
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 22
NspV TTCGAA 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 72
PciI ACATGT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 288
Pfl23II CGTACG 1 cut(s) 137
PkrI GCNGC 7 cut(s) 130, 154, 157, 211, 214, 427, 535
PluTI GGCGCC 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 611
PpuMI RGGWCCY 2 cut(s) 7, 95
PscI ACATGT 1 cut(s) 18
Psp5II RGGWCCY 2 cut(s) 7, 95
Psp6I CCWGG 1 cut(s) 505
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 505
PspLI CGTACG 1 cut(s) 137
PspN4I GGNNCC 6 cut(s) 9, 97, 103, 165, 259, 644
PspPI GGNCC 7 cut(s) 7, 87, 95, 101, 512, 629, 643
PspPPI RGGWCCY 2 cut(s) 7, 95
PspXI VCTCGAGB 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 3 cut(s) 139, 354, 734
RsaNI GTAC 3 cut(s) 138, 353, 733
SacII CCGCGG 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 2 cut(s) 309, 432
SatI GCNGC 7 cut(s) 129, 153, 156, 210, 213, 426, 534
Sau3AI GATC 4 cut(s) 59, 199, 374, 709
Sau96I GGNCC 7 cut(s) 7, 87, 95, 101, 512, 629, 643
ScrFI CCNGG 4 cut(s) 86, 99, 221, 507
SfaNI GCATC 2 cut(s) 406, 706
SfoI GGCGCC 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 72
Sfr303I CCGCGG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 557
SgrBI CCGCGG 1 cut(s) 429
SinI GGWCC 5 cut(s) 7, 95, 101, 512, 629
SlaI CTCGAG 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 72, 225
SmoI CTYRAG 2 cut(s) 72, 225
Sse9I AATT 1 cut(s) 553
SspDI GGCGCC 1 cut(s) 163
SspMI CTAG 2 cut(s) 570, 633
StyD4I CCNGG 4 cut(s) 84, 97, 219, 505
StyI CCWWGG 2 cut(s) 159, 569
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 3 cut(s) 607, 613, 655
TaqI TCGA 5 cut(s) 27, 73, 282, 377, 557
TaqII GACCGA 1 cut(s) 378
TasI AATT 1 cut(s) 553
TatI WGTACW 1 cut(s) 352
TauI GCSGC 6 cut(s) 131, 155, 158, 212, 215, 428
Tru1I TTAA 2 cut(s) 309, 432
Tru9I TTAA 2 cut(s) 309, 432
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 1 cut(s) 533
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 1 cut(s) 212
VpaK11BI GGWCC 5 cut(s) 7, 95, 101, 512, 629
XapI RAATTY 1 cut(s) 553
XceI RCATGY 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 72
XmaJI CCTAGG 1 cut(s) 569
XmiI GTMKAC 2 cut(s) 194, 607
XspI CTAG 2 cut(s) 570, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.