Rh5DG446100

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005413
Physical Location & Seq
Forward (+)
71234261 .. 71239438
5178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG446100.1

Sequence Viewer

Length: 615 bp
ATGAATGATTTGGGGATTCAGGCGCCGGAGTCCACGTGGCAGCACGACATGTTCTCGGATCAGGGAGCGGCCTACGCAGCCTCGGCTGGCAGAGCCTCCGCCATCGAAACTGGAACCAAGCTCTACATCTCTAATCTCGACTATGGCGTTTCCAACGAGGACATCAAGGAGCTGTTTGTTGAGGTTGGTGACCTGAAACGTTATGGAGTGCATTATGATAGAAGTGGCAGATCAAAGGGAACAGCAGATGTAGTCTTTTCACGAAGACAGGATGCTGTGGCGGCTGTTAAAAGATACAACAATGTAATGCTTGATGGGAAACCGATGAAGATAGAGATCGTAGGAACAAACATTGCAACACCTGGTGCACCTTCCGCTATGCCACCCGCTGCAAATGGTGCTTTCAGAAATTCAAATGGTGGAGCTCCCCGAGGTGGACAGGGTAGAGGTGGCGCATTTGGACGCCCTCGTGGTGGGGGTGGGGGTGGCCGTGGTCCTAGAAGGGGCCGTGGTGGACGTGGAAGAGGAGGGCGTAGTGGTGAAAAGGTTTCTGCTGAAGATCTTGATGCTGAGCTGGAGAAGTACCATGCAACTGCAGAAACAATGCAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

204

Amino Acids

21.43

Weight (kDa)

9.36

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 41 - 109 6e-14 RNA recognition motif
FoP_duplication PF13865 138 - 197 3.9e-09 C-terminal duplication domain of Friend of PRMT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 68
AciI CCGC 5 cut(s) 68, 99, 281, 375, 387
AclI AACGTT 1 cut(s) 199
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 1 cut(s) 409
AcuI CTGAAG 1 cut(s) 576
AcvI CACGTG 1 cut(s) 36
AcyI GRCGYC 2 cut(s) 23, 463
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 1 cut(s) 584
AfiI CCNNNNNNNGG 3 cut(s) 434, 473, 503
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 1 cut(s) 414
AjiI CACGTC 1 cut(s) 518
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 4 cut(s) 121, 172, 425, 574
AluI AGCT 4 cut(s) 121, 172, 425, 574
Alw21I GWGCWC 2 cut(s) 370, 427
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 3 cut(s) 69, 487, 505
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 3 cut(s) 40, 77, 389
ApoI RAATTY 1 cut(s) 409
AspLEI GCGC 2 cut(s) 25, 455
AspS9I GGNCC 2 cut(s) 494, 505
AsuHPI GGTGA 2 cut(s) 200, 551
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 427
BauI CACGAG 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 2 cut(s) 370, 427
BbvI GCAGC 3 cut(s) 52, 89, 376
BccI CCATC 2 cut(s) 110, 308
BceAI ACGGC 2 cut(s) 474, 492
BciT130I CCWGG 1 cut(s) 363
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 26
BglII AGATCT 1 cut(s) 559
BisI GCNGC 5 cut(s) 41, 69, 78, 282, 390
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 5 cut(s) 42, 70, 79, 283, 391
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 494
BmeT110I CYCGRG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 494, 505
BmiI GGNNCC 3 cut(s) 24, 115, 506
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 262, 556
BpiI GAAGAC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 596
Bpu1102I GCTNAGC 1 cut(s) 570
BsaAI YACGTR 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 335
BsaHI GRCGYC 2 cut(s) 23, 463
BsaJI CCNNGG 4 cut(s) 81, 430, 490, 508
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 473, 503
Bse1I ACTGG 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 335
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 4 cut(s) 81, 430, 490, 508
BseGI GGATG 1 cut(s) 277
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 3 cut(s) 434, 473, 503
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 561
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 3 cut(s) 52, 89, 376
BshFI GGCC 3 cut(s) 71, 489, 507
BshNI GGYRCC 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 370, 427
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 3 cut(s) 434, 473, 503
BsnI GGCC 3 cut(s) 71, 489, 507
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 370, 427
Bsp143I GATC 4 cut(s) 58, 230, 336, 559
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 5 cut(s) 68, 99, 281, 375, 387
BspANI GGCC 3 cut(s) 71, 489, 507
BspCNI CTCAG 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 24, 115, 506
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 4 cut(s) 81, 430, 490, 508
BssMI GATC 4 cut(s) 58, 230, 336, 559
BssNI GRCGYC 2 cut(s) 23, 463
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 517
BstACI GRCGYC 2 cut(s) 23, 463
BstAPI GCANNNNNTGC 1 cut(s) 398
BstBAI YACGTR 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 570
BstDSI CCRYGG 2 cut(s) 490, 508
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 1 cut(s) 277
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 2 cut(s) 25, 455
BstKTI GATC 4 cut(s) 61, 233, 339, 562
BstMBI GATC 4 cut(s) 58, 230, 336, 559
BstMWI GCNNNNNNNGC 7 cut(s) 74, 77, 83, 92, 281, 374, 398
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 52
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 52, 89, 376
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 3 cut(s) 71, 489, 507
BtgI CCRYGG 2 cut(s) 490, 508
BtrI CACGTC 1 cut(s) 518
BtsCI GGATG 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 88
CfoI GCGC 2 cut(s) 25, 455
Cfr13I GGNCC 2 cut(s) 494, 505
CseI GACGC 1 cut(s) 471
CsiI ACCWGGT 1 cut(s) 361
Csp6I GTAC 1 cut(s) 583
CviAII CATG 2 cut(s) 49, 587
CviQI GTAC 1 cut(s) 583
DdeI CTNAG 1 cut(s) 570
DinI GGCGCC 1 cut(s) 24
DpnI GATC 4 cut(s) 60, 232, 338, 561
DpnII GATC 4 cut(s) 58, 230, 336, 559
DraIII CACNNNGTG 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 425
Eco24I GRGCYC 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 576
Eco72I CACGTG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 429
Eco91I GGTNACC 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 427
EgeI GGCGCC 1 cut(s) 24
EheI GGCGCC 1 cut(s) 24
FaeI CATG 2 cut(s) 52, 590
FaiI YATR 6 cut(s) 50, 144, 204, 216, 380, 588
FatI CATG 2 cut(s) 48, 586
FauI CCCGC 1 cut(s) 394
Fnu4HI GCNGC 5 cut(s) 41, 69, 78, 282, 390
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 5 cut(s) 41, 69, 78, 282, 390
FspBI CTAG 1 cut(s) 498
GlaI GCGC 2 cut(s) 24, 454
GluI GCNGC 5 cut(s) 41, 69, 78, 282, 390
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 3 cut(s) 71, 489, 507
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 471
HhaI GCGC 2 cut(s) 25, 455
Hin1I GRCGYC 2 cut(s) 23, 463
Hin1II CATG 2 cut(s) 52, 590
Hin6I GCGC 2 cut(s) 23, 453
HinP1I GCGC 2 cut(s) 23, 453
HinfI GANTC 2 cut(s) 16, 29
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 200, 551
Hpy166II GTNNAC 4 cut(s) 33, 368, 437, 515
Hpy188I TCNGA 2 cut(s) 58, 407
Hpy188III TCNNGA 3 cut(s) 137, 261, 563
Hpy8I GTNNAC 4 cut(s) 33, 368, 437, 515
HpyAV CCTTC 2 cut(s) 381, 495
HpyCH4IV ACGT 3 cut(s) 35, 199, 517
HpyCH4V TGCA 7 cut(s) 211, 356, 368, 392, 590, 596, 607
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 77, 83, 92, 281, 374, 398
HpyF3I CTNAG 1 cut(s) 570
HpySE526I ACGT 3 cut(s) 35, 199, 517
Hsp92I GRCGYC 2 cut(s) 23, 463
Hsp92II CATG 2 cut(s) 52, 590
HspAI GCGC 2 cut(s) 23, 453
KasI GGCGCC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 58, 230, 336, 559
LmnI GCTCC 4 cut(s) 65, 169, 422, 430
Lsp1109I GCAGC 3 cut(s) 52, 89, 376
LweI GCATC 2 cut(s) 262, 556
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 1 cut(s) 498
MaeII ACGT 3 cut(s) 35, 199, 517
MaeIII GTNAC 1 cut(s) 188
MalI GATC 4 cut(s) 60, 232, 338, 561
MbiI CCGCTC 1 cut(s) 68
MboI GATC 4 cut(s) 58, 230, 336, 559
MboII GAAGA 4 cut(s) 276, 340, 534, 569
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 370, 427
MluCI AATT 1 cut(s) 409
Mly113I GGCGCC 1 cut(s) 23
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 9 cut(s) 91, 106, 151, 175, 425, 440, 477, 518, 521
MseI TTAA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 7 cut(s) 74, 77, 83, 92, 281, 374, 398
NarI GGCGCC 1 cut(s) 23
NdeII GATC 4 cut(s) 58, 230, 336, 559
NlaIII CATG 2 cut(s) 52, 590
NlaIV GGNNCC 3 cut(s) 24, 115, 506
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 52
PciI ACATGT 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 5 cut(s) 42, 70, 79, 283, 391
PleI GAGTC 1 cut(s) 37
PluTI GGCGCC 1 cut(s) 26
PmaCI CACGTG 1 cut(s) 36
PmlI CACGTG 1 cut(s) 36
PpsI GAGTC 1 cut(s) 37
Ppu21I YACGTR 1 cut(s) 36
PscI ACATGT 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 427
Psp1406I AACGTT 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 361
PspCI CACGTG 1 cut(s) 36
PspEI GGTNACC 1 cut(s) 188
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 3 cut(s) 24, 115, 506
PspPI GGNCC 2 cut(s) 494, 505
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
SacI GAGCTC 1 cut(s) 427
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 5 cut(s) 41, 69, 78, 282, 390
Sau3AI GATC 4 cut(s) 58, 230, 336, 559
Sau96I GGNCC 2 cut(s) 494, 505
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 370, 427
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 2 cut(s) 262, 556
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 1 cut(s) 24
SinI GGWCC 1 cut(s) 494
Sse9I AATT 1 cut(s) 409
SsiI CCGC 5 cut(s) 68, 99, 281, 375, 387
SspDI GGCGCC 1 cut(s) 22
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 361
TaiI ACGT 3 cut(s) 38, 202, 520
TaqI TCGA 2 cut(s) 105, 138
TasI AATT 1 cut(s) 409
TauI GCSGC 2 cut(s) 71, 284
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 3 cut(s) 40, 77, 389
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 2 cut(s) 17, 341
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 409
XceI RCATGY 1 cut(s) 52
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.