Rroxscaffold_1G00017180

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005413
Physical Location & Seq
Reverse (-)
21349360 .. 21354034
4675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00017180.1

Sequence Viewer

Length: 759 bp
ATGGATCATCTAAACATGTCCCTCGACGACATCATCAAGACCTCCAAGAAACCCGGATCCGGCAACAGCCGCGGCCGCGCCCGGGCTGCCTCCGGTCCCGGACCCGCCCGCCGCTTGCCCAATCGCGCCGGAAATCGTGCGACGCCCTATGGCGCCGGCGCCGCCAAGGCGCCGGAGGCCACGTGGCAGCACGACATGTTCTCGGATCAGGGAGCGGCCTACGGAGCCCCGGCTGGCAGAGCCTCCGCCATCGAAACTGGAACCAAGCTCTACATCTCTAATCTCGACTATGGCGTTTCCAACGAGGACATCAAGGAGCTGTTTGTTGAGGTTGGTGACCTGAAACGTTATGGAGTGCATTATGATAGAAGTGGCAGATCAAAGGGAACGGCAGATGTAGTCTTTTCACGAAGACAGGATGCTGTGGCGGCTGTTAAAAGATACAACAATGTAATGCTTGATGGGAAACCGATGAAGATAGAGATCGTAGGAACAAACATTGCAACACCTGGTGCGCCTTCCGCTGTGCCTCCTGCTGCAAATGGCGCTTTCAGAAATTCAAATGGAGCTCCCCGAGGTGGACAGGGTAGAGGTGGCGCATTTGGACGCCCTCGTGGTGGGGGTGGGGGTGGCCGTGGTCCTAGAAGGGGCCGTGGTGGACGTGGAAGAGGAGGGCGTAGTGGTGAAAAGGTTTCTGCTGAAGATCTTGATGCTGAGCTGGAGAAGTACCATGCAACTGCAGAAACAATGCAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

252

Amino Acids

26.31

Weight (kDa)

10.1

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 90 - 158 9.1e-14 RNA recognition motif
FoP_duplication PF13865 187 - 245 6.1e-10 C-terminal duplication domain of Friend of PRMT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 152, 158, 169
AccBSI CCGCTC 1 cut(s) 215
AccII CGCG 3 cut(s) 72, 78, 126
AclI AACGTT 1 cut(s) 346
AclWI GGATC 4 cut(s) 12, 51, 64, 213
AcoI YGGCCR 2 cut(s) 73, 631
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 720
AcvI CACGTG 1 cut(s) 183
AcyI GRCGYC 5 cut(s) 143, 153, 159, 170, 607
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 5 cut(s) 59, 82, 578, 617, 647
AflIII ACRYGT 2 cut(s) 15, 195
AgsI TTSAA 1 cut(s) 561
AjiI CACGTC 1 cut(s) 662
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 4 cut(s) 268, 319, 569, 718
AluI AGCT 4 cut(s) 268, 319, 569, 718
Alw21I GWGCWC 1 cut(s) 571
AlwI GGATC 4 cut(s) 12, 51, 64, 213
Ama87I CYCGRG 2 cut(s) 81, 573
AoxI GGCC 5 cut(s) 73, 177, 216, 631, 649
ApeKI GCWGC 3 cut(s) 86, 187, 536
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 8 cut(s) 80, 128, 155, 161, 172, 517, 548, 599
AspS9I GGNCC 4 cut(s) 95, 101, 638, 649
AsuC2I CCSGG 5 cut(s) 54, 82, 83, 99, 230
AsuHPI GGTGA 2 cut(s) 347, 695
AvaI CYCGRG 2 cut(s) 81, 573
AvaII GGWCC 3 cut(s) 95, 101, 638
BamHI GGATCC 1 cut(s) 56
BanI GGYRCC 3 cut(s) 152, 158, 169
BanII GRGCYC 2 cut(s) 229, 571
BauI CACGAG 1 cut(s) 612
BbrPI CACGTG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 3 cut(s) 73, 199, 523
BccI CCATC 2 cut(s) 257, 455
BceAI ACGGC 3 cut(s) 405, 618, 636
BciT130I CCWGG 1 cut(s) 510
BcnI CCSGG 5 cut(s) 54, 82, 83, 99, 230
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 738
BfoI RGCGCY 4 cut(s) 156, 162, 173, 549
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 703
BlpI GCTNAGC 1 cut(s) 714
Bme1390I CCNGG 6 cut(s) 54, 82, 83, 99, 230, 510
Bme18I GGWCC 3 cut(s) 95, 101, 638
BmeT110I CYCGRG 2 cut(s) 81, 573
BmgBI CACGTC 1 cut(s) 662
BmgT120I GGNCC 4 cut(s) 95, 101, 638, 649
BmiI GGNNCC 9 cut(s) 58, 97, 103, 154, 160, 171, 226, 262, 650
BmrFI CCNGG 6 cut(s) 54, 82, 83, 99, 230, 510
BmsI GCATC 2 cut(s) 409, 700
BpiI GAAGAC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 740
Bpu1102I GCTNAGC 1 cut(s) 714
BpuMI CCSGG 5 cut(s) 54, 82, 83, 99, 230
BsaAI YACGTR 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 482
BsaHI GRCGYC 5 cut(s) 143, 153, 159, 170, 607
BsaJI CCNNGG 7 cut(s) 70, 81, 165, 228, 574, 634, 652
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 82, 578, 617, 647
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 482
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 7 cut(s) 70, 81, 165, 228, 574, 634, 652
BseGI GGATG 1 cut(s) 424
BseJI GATNNNNATC 1 cut(s) 482
BseLI CCNNNNNNNGG 5 cut(s) 59, 82, 578, 617, 647
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 684
BseX3I CGGCCG 1 cut(s) 73
BseXI GCAGC 3 cut(s) 73, 199, 523
Bsh1236I CGCG 3 cut(s) 72, 78, 126
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 75, 179, 218, 633, 651
BshNI GGYRCC 3 cut(s) 152, 158, 169
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 571
BsiHKCI CYCGRG 2 cut(s) 81, 573
BsiSI CCGG 9 cut(s) 54, 60, 82, 93, 99, 129, 156, 173, 230
BslFI GGGAC 2 cut(s) 4, 81
BslI CCNNNNNNNGG 5 cut(s) 59, 82, 578, 617, 647
BsmFI GGGAC 2 cut(s) 4, 81
BsnI GGCC 5 cut(s) 75, 179, 218, 633, 651
BsoBI CYCGRG 2 cut(s) 81, 573
Bsp1286I GDGCHC 2 cut(s) 229, 571
Bsp143I GATC 6 cut(s) 4, 56, 205, 377, 483, 703
Bsp1720I GCTNAGC 1 cut(s) 714
BspANI GGCC 5 cut(s) 75, 179, 218, 633, 651
BspCNI CTCAG 1 cut(s) 706
BspFNI CGCG 3 cut(s) 72, 78, 126
BspLI GGNNCC 9 cut(s) 58, 97, 103, 154, 160, 171, 226, 262, 650
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 4 cut(s) 12, 51, 64, 213
BspT107I GGYRCC 3 cut(s) 152, 158, 169
BsrBI CCGCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 7 cut(s) 70, 81, 165, 228, 574, 634, 652
BssMI GATC 6 cut(s) 4, 56, 205, 377, 483, 703
BssNI GRCGYC 5 cut(s) 143, 153, 159, 170, 607
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 510
Bst6I CTCTTC 1 cut(s) 661
BstACI GRCGYC 5 cut(s) 143, 153, 159, 170, 607
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 4 cut(s) 109, 116, 157, 235
BstDEI CTNAG 1 cut(s) 714
BstDSI CCRYGG 3 cut(s) 70, 634, 652
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 3 cut(s) 72, 78, 126
BstH2I RGCGCY 4 cut(s) 156, 162, 173, 549
BstHHI GCGC 8 cut(s) 80, 128, 155, 161, 172, 517, 548, 599
BstKTI GATC 6 cut(s) 7, 59, 208, 380, 486, 706
BstMBI GATC 6 cut(s) 4, 56, 205, 377, 483, 703
BstMCI CGRYCG 1 cut(s) 76
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 2 cut(s) 19, 199
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 6 cut(s) 52, 80, 81, 97, 228, 508
BstSFI CTRYAG 1 cut(s) 738
BstUI CGCG 3 cut(s) 72, 78, 126
BstV1I GCAGC 3 cut(s) 73, 199, 523
BstV2I GAAGAC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 56, 703
BstYI RGATCY 2 cut(s) 56, 703
BstZI CGGCCG 1 cut(s) 73
BsuRI GGCC 5 cut(s) 75, 179, 218, 633, 651
BtgI CCRYGG 3 cut(s) 70, 634, 652
BtrI CACGTC 1 cut(s) 662
BtsCI GGATG 1 cut(s) 424
Cac8I GCNNGC 4 cut(s) 109, 116, 157, 235
CciNI GCGGCCGC 1 cut(s) 73
CfoI GCGC 8 cut(s) 80, 128, 155, 161, 172, 517, 548, 599
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 95, 101, 638, 649
Cfr42I CCGCGG 1 cut(s) 73
Cfr9I CCCGGG 1 cut(s) 81
CseI GACGC 2 cut(s) 151, 615
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 1 cut(s) 727
CviAII CATG 3 cut(s) 16, 196, 731
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 1 cut(s) 714
DinI GGCGCC 3 cut(s) 154, 160, 171
DpnI GATC 6 cut(s) 6, 58, 207, 379, 485, 705
DpnII GATC 6 cut(s) 4, 56, 205, 377, 483, 703
DraIII CACNNNGTG 1 cut(s) 512
EaeI YGGCCR 2 cut(s) 73, 631
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 661
EciI GGCGGA 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 569
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 229, 571
Eco47I GGWCC 3 cut(s) 95, 101, 638
Eco52I CGGCCG 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 720
Eco72I CACGTG 1 cut(s) 183
Eco88I CYCGRG 2 cut(s) 81, 573
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 569
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 2 cut(s) 229, 571
EgeI GGCGCC 3 cut(s) 154, 160, 171
EheI GGCGCC 3 cut(s) 154, 160, 171
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 3 cut(s) 19, 199, 734
FaiI YATR 7 cut(s) 17, 150, 197, 291, 351, 363, 732
FaqI GGGAC 2 cut(s) 4, 81
FatI CATG 3 cut(s) 15, 195, 730
FauI CCCGC 2 cut(s) 112, 116
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 2 cut(s) 229, 571
FspBI CTAG 1 cut(s) 642
GlaI GCGC 8 cut(s) 79, 127, 154, 160, 171, 516, 547, 598
GsuI CTGGAG 1 cut(s) 740
HaeII RGCGCY 4 cut(s) 156, 162, 173, 549
HaeIII GGCC 5 cut(s) 75, 179, 218, 633, 651
HapII CCGG 9 cut(s) 54, 60, 82, 93, 99, 129, 156, 173, 230
HgaI GACGC 2 cut(s) 151, 615
HhaI GCGC 8 cut(s) 80, 128, 155, 161, 172, 517, 548, 599
Hin1I GRCGYC 5 cut(s) 143, 153, 159, 170, 607
Hin1II CATG 3 cut(s) 19, 199, 734
Hin6I GCGC 8 cut(s) 78, 126, 153, 159, 170, 515, 546, 597
HinP1I GCGC 8 cut(s) 78, 126, 153, 159, 170, 515, 546, 597
HpaII CCGG 9 cut(s) 54, 60, 82, 93, 99, 129, 156, 173, 230
HphI GGTGA 2 cut(s) 347, 695
Hpy166II GTNNAC 2 cut(s) 581, 659
Hpy188I TCNGA 2 cut(s) 205, 554
Hpy188III TCNNGA 4 cut(s) 37, 284, 408, 707
Hpy8I GTNNAC 2 cut(s) 581, 659
Hpy99I CGWCG 2 cut(s) 29, 145
HpyAV CCTTC 2 cut(s) 528, 639
HpyCH4IV ACGT 3 cut(s) 182, 346, 661
HpyCH4V TGCA 6 cut(s) 358, 503, 539, 734, 740, 751
HpyF3I CTNAG 1 cut(s) 714
HpySE526I ACGT 3 cut(s) 182, 346, 661
Hsp92I GRCGYC 5 cut(s) 143, 153, 159, 170, 607
Hsp92II CATG 3 cut(s) 19, 199, 734
HspAI GCGC 8 cut(s) 78, 126, 153, 159, 170, 515, 546, 597
KasI GGCGCC 3 cut(s) 152, 158, 169
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 6 cut(s) 4, 56, 205, 377, 483, 703
LmnI GCTCC 5 cut(s) 212, 224, 316, 566, 574
Lsp1109I GCAGC 3 cut(s) 73, 199, 523
LweI GCATC 2 cut(s) 409, 700
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 1 cut(s) 642
MaeII ACGT 3 cut(s) 182, 346, 661
MaeIII GTNAC 1 cut(s) 335
MalI GATC 6 cut(s) 6, 58, 207, 379, 485, 705
MbiI CCGCTC 1 cut(s) 215
MboI GATC 6 cut(s) 4, 56, 205, 377, 483, 703
MboII GAAGA 4 cut(s) 423, 487, 678, 713
MflI RGATCY 2 cut(s) 56, 703
MhlI GDGCHC 2 cut(s) 229, 571
MluCI AATT 1 cut(s) 556
Mly113I GGCGCC 3 cut(s) 153, 159, 170
MmeI TCCRAC 1 cut(s) 324
MreI CGCCGGCG 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 155
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 72, 524
MspI CCGG 9 cut(s) 54, 60, 82, 93, 99, 129, 156, 173, 230
MspR9I CCNGG 6 cut(s) 54, 82, 83, 99, 230, 510
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 3 cut(s) 72, 78, 126
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 3 cut(s) 153, 159, 170
NciI CCSGG 5 cut(s) 54, 82, 83, 99, 230
NdeII GATC 6 cut(s) 4, 56, 205, 377, 483, 703
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 3 cut(s) 19, 199, 734
NlaIV GGNNCC 9 cut(s) 58, 97, 103, 154, 160, 171, 226, 262, 650
NmuCI GTSAC 1 cut(s) 335
NotI GCGGCCGC 1 cut(s) 73
NspI RCATGY 2 cut(s) 19, 199
PciI ACATGT 2 cut(s) 15, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 97
PluTI GGCGCC 3 cut(s) 156, 162, 173
PmaCI CACGTG 1 cut(s) 183
PmlI CACGTG 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 2 cut(s) 15, 195
Psp124BI GAGCTC 1 cut(s) 571
Psp1406I AACGTT 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 508
PspCI CACGTG 1 cut(s) 183
PspEI GGTNACC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 9 cut(s) 58, 97, 103, 154, 160, 171, 226, 262, 650
PspPI GGNCC 4 cut(s) 95, 101, 638, 649
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 2 cut(s) 56, 703
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
SacI GAGCTC 1 cut(s) 571
SacII CCGCGG 1 cut(s) 73
SaqAI TTAA 1 cut(s) 435
Sau3AI GATC 6 cut(s) 4, 56, 205, 377, 483, 703
Sau96I GGNCC 4 cut(s) 95, 101, 638, 649
ScrFI CCNGG 6 cut(s) 54, 82, 83, 99, 230, 510
SduI GDGCHC 2 cut(s) 229, 571
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 2 cut(s) 409, 700
SfcI CTRYAG 1 cut(s) 738
SfoI GGCGCC 3 cut(s) 154, 160, 171
Sfr303I CCGCGG 1 cut(s) 73
SgrAI CRCCGGYG 1 cut(s) 155
SgrBI CCGCGG 1 cut(s) 73
SinI GGWCC 3 cut(s) 95, 101, 638
SmaI CCCGGG 1 cut(s) 83
SrfI GCCCGGGC 1 cut(s) 83
Sse9I AATT 1 cut(s) 556
SspDI GGCGCC 3 cut(s) 152, 158, 169
SspMI CTAG 1 cut(s) 642
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 6 cut(s) 52, 80, 81, 97, 228, 508
StyI CCWWGG 1 cut(s) 165
TaiI ACGT 3 cut(s) 185, 349, 664
TaqI TCGA 3 cut(s) 24, 252, 285
TasI AATT 1 cut(s) 556
TauI GCSGC 7 cut(s) 72, 75, 78, 114, 164, 218, 431
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 3 cut(s) 86, 187, 536
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 237
TspMI CCCGGG 1 cut(s) 81
VpaK11BI GGWCC 3 cut(s) 95, 101, 638
XapI RAATTY 1 cut(s) 556
XceI RCATGY 2 cut(s) 19, 199
XmaI CCCGGG 1 cut(s) 81
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.