Rmu_ssc0000309.1_g000049

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000309.1
OG0005413
Physical Location & Seq
Reverse (-)
201533 .. 204052
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000309.1_g000049.1.cds

Sequence Viewer

Length: 762 bp
atggatcatctaaacatgtccctcgacgacatcatcaagacctccaagaaacccggatccggcaacagccgcggccgcgcccgggctgcctccggtcccggacccgcccgccgcttgcccaatcgcgccggaaatcgtgcgacgccctatggcgccggcgccgccaaggcgccggagtccacgtggcagcacgacatgttctcggatcagggagcggcctacgcagcctcggctggcagagcctccgccatcgaaactggaaccaagctctacatctctaatctcgactatggcgtttccaacgaggacatcaaggagctgtttgttgaggttggtgacctgaaacgttatggagtgcattatgatagaagtggcagatcaaagggaacagcagatgtagtcttttcacgaagacaggatgctgtggcggctgttaaaagatacaacaatgtaatgcttgatgggaaaccgatgaagatagagatcgtaggaacaaacattgcaacacctggtgcaccttccgctatgccacccgctgcaaatggtgctttcagaaattcaaatggtggagctccccgaggtggacagggtagaggtggcgcatttggacgccctcgtggtgggggtgggggtggccgtggtcctagaaggggccgtggtggacgtggaagaggagggcgtagtggtgaaaaggtttctgctgaagatcttgatgctgagctggagaagtaccatgcaactgcagaaacaatgcaagagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

253

Amino Acids

26.42

Weight (kDa)

10.1

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 152, 158, 169
AccBSI CCGCTC 1 cut(s) 215
AccII CGCG 3 cut(s) 72, 78, 126
AclI AACGTT 1 cut(s) 346
AclWI GGATC 4 cut(s) 12, 51, 64, 213
AcoI YGGCCR 2 cut(s) 73, 634
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 723
AcvI CACGTG 1 cut(s) 183
AcyI GRCGYC 5 cut(s) 143, 153, 159, 170, 610
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 5 cut(s) 59, 82, 581, 620, 650
AflIII ACRYGT 2 cut(s) 15, 195
AgsI TTSAA 1 cut(s) 561
AjiI CACGTC 1 cut(s) 665
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 4 cut(s) 268, 319, 572, 721
AluI AGCT 4 cut(s) 268, 319, 572, 721
Alw21I GWGCWC 2 cut(s) 517, 574
Alw44I GTGCAC 1 cut(s) 513
AlwI GGATC 4 cut(s) 12, 51, 64, 213
Ama87I CYCGRG 2 cut(s) 81, 576
AoxI GGCC 4 cut(s) 73, 216, 634, 652
ApaLI GTGCAC 1 cut(s) 513
ApeKI GCWGC 4 cut(s) 86, 187, 224, 536
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 6 cut(s) 80, 128, 155, 161, 172, 602
AspS9I GGNCC 4 cut(s) 95, 101, 641, 652
AsuC2I CCSGG 4 cut(s) 54, 82, 83, 99
AsuHPI GGTGA 2 cut(s) 347, 698
AvaI CYCGRG 2 cut(s) 81, 576
AvaII GGWCC 3 cut(s) 95, 101, 641
BaeGI GKGCMC 1 cut(s) 517
BamHI GGATCC 1 cut(s) 56
BanI GGYRCC 3 cut(s) 152, 158, 169
BanII GRGCYC 1 cut(s) 574
BauI CACGAG 1 cut(s) 615
BbrPI CACGTG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 418
Bbv12I GWGCWC 2 cut(s) 517, 574
BbvI GCAGC 4 cut(s) 73, 199, 236, 523
BccI CCATC 2 cut(s) 257, 455
BceAI ACGGC 2 cut(s) 621, 639
BciT130I CCWGG 1 cut(s) 510
BcnI CCSGG 4 cut(s) 54, 82, 83, 99
BfaI CTAG 1 cut(s) 645
BfmI CTRYAG 1 cut(s) 741
BfoI RGCGCY 3 cut(s) 156, 162, 173
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 706
BlpI GCTNAGC 1 cut(s) 717
Bme1390I CCNGG 5 cut(s) 54, 82, 83, 99, 510
Bme18I GGWCC 3 cut(s) 95, 101, 641
BmeT110I CYCGRG 2 cut(s) 81, 576
BmgBI CACGTC 1 cut(s) 665
BmgT120I GGNCC 4 cut(s) 95, 101, 641, 652
BmiI GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 653
BmrFI CCNGG 5 cut(s) 54, 82, 83, 99, 510
BmsI GCATC 2 cut(s) 409, 703
BpiI GAAGAC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 743
Bpu1102I GCTNAGC 1 cut(s) 717
BpuMI CCSGG 4 cut(s) 54, 82, 83, 99
BsaAI YACGTR 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 482
BsaHI GRCGYC 5 cut(s) 143, 153, 159, 170, 610
BsaJI CCNNGG 7 cut(s) 70, 81, 165, 228, 577, 637, 655
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 82, 581, 620, 650
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 482
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 7 cut(s) 70, 81, 165, 228, 577, 637, 655
BseGI GGATG 1 cut(s) 424
BseJI GATNNNNATC 1 cut(s) 482
BseLI CCNNNNNNNGG 5 cut(s) 59, 82, 581, 620, 650
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 708
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 687
BseSI GKGCMC 1 cut(s) 517
BseX3I CGGCCG 1 cut(s) 73
BseXI GCAGC 4 cut(s) 73, 199, 236, 523
Bsh1236I CGCG 3 cut(s) 72, 78, 126
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 4 cut(s) 75, 218, 636, 654
BshNI GGYRCC 3 cut(s) 152, 158, 169
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 517, 574
BsiHKCI CYCGRG 2 cut(s) 81, 576
BsiSI CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
BslFI GGGAC 2 cut(s) 4, 81
BslI CCNNNNNNNGG 5 cut(s) 59, 82, 581, 620, 650
BsmFI GGGAC 2 cut(s) 4, 81
BsnI GGCC 4 cut(s) 75, 218, 636, 654
BsoBI CYCGRG 2 cut(s) 81, 576
Bsp1286I GDGCHC 2 cut(s) 517, 574
Bsp143I GATC 6 cut(s) 4, 56, 205, 377, 483, 706
Bsp1720I GCTNAGC 1 cut(s) 717
BspANI GGCC 4 cut(s) 75, 218, 636, 654
BspCNI CTCAG 1 cut(s) 709
BspFNI CGCG 3 cut(s) 72, 78, 126
BspLI GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 653
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 4 cut(s) 12, 51, 64, 213
BspT107I GGYRCC 3 cut(s) 152, 158, 169
BsrBI CCGCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 7 cut(s) 70, 81, 165, 228, 577, 637, 655
BssMI GATC 6 cut(s) 4, 56, 205, 377, 483, 706
BssNI GRCGYC 5 cut(s) 143, 153, 159, 170, 610
BssSI CACGAG 1 cut(s) 615
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 615
Bst2UI CCWGG 1 cut(s) 510
Bst6I CTCTTC 1 cut(s) 664
BstACI GRCGYC 5 cut(s) 143, 153, 159, 170, 610
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 4 cut(s) 109, 116, 157, 235
BstDEI CTNAG 1 cut(s) 717
BstDSI CCRYGG 3 cut(s) 70, 637, 655
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 3 cut(s) 72, 78, 126
BstH2I RGCGCY 3 cut(s) 156, 162, 173
BstHHI GCGC 6 cut(s) 80, 128, 155, 161, 172, 602
BstKTI GATC 6 cut(s) 7, 59, 208, 380, 486, 709
BstMBI GATC 6 cut(s) 4, 56, 205, 377, 483, 706
BstMCI CGRYCG 1 cut(s) 76
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 2 cut(s) 19, 199
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 5 cut(s) 52, 80, 81, 97, 508
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 1 cut(s) 517
BstUI CGCG 3 cut(s) 72, 78, 126
BstV1I GCAGC 4 cut(s) 73, 199, 236, 523
BstV2I GAAGAC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 56, 706
BstYI RGATCY 2 cut(s) 56, 706
BstZI CGGCCG 1 cut(s) 73
BsuRI GGCC 4 cut(s) 75, 218, 636, 654
BtgI CCRYGG 3 cut(s) 70, 637, 655
BtrI CACGTC 1 cut(s) 665
BtsCI GGATG 1 cut(s) 424
Cac8I GCNNGC 4 cut(s) 109, 116, 157, 235
CciNI GCGGCCGC 1 cut(s) 73
CfoI GCGC 6 cut(s) 80, 128, 155, 161, 172, 602
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 95, 101, 641, 652
Cfr42I CCGCGG 1 cut(s) 73
Cfr9I CCCGGG 1 cut(s) 81
CseI GACGC 2 cut(s) 151, 618
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 1 cut(s) 730
CviAII CATG 3 cut(s) 16, 196, 734
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 1 cut(s) 717
DinI GGCGCC 3 cut(s) 154, 160, 171
DpnI GATC 6 cut(s) 6, 58, 207, 379, 485, 708
DpnII GATC 6 cut(s) 4, 56, 205, 377, 483, 706
DraIII CACNNNGTG 1 cut(s) 512
EaeI YGGCCR 2 cut(s) 73, 634
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
EciI GGCGGA 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 572
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 574
Eco47I GGWCC 3 cut(s) 95, 101, 641
Eco52I CGGCCG 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 572
Eco57I CTGAAG 1 cut(s) 723
Eco72I CACGTG 1 cut(s) 183
Eco88I CYCGRG 2 cut(s) 81, 576
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 572
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 574
EgeI GGCGCC 3 cut(s) 154, 160, 171
EheI GGCGCC 3 cut(s) 154, 160, 171
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 3 cut(s) 19, 199, 737
FaiI YATR 8 cut(s) 17, 150, 197, 291, 351, 363, 527, 735
FaqI GGGAC 2 cut(s) 4, 81
FatI CATG 3 cut(s) 15, 195, 733
FauI CCCGC 3 cut(s) 112, 116, 541
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 574
FspBI CTAG 1 cut(s) 645
GlaI GCGC 6 cut(s) 79, 127, 154, 160, 171, 601
GsuI CTGGAG 1 cut(s) 743
HaeII RGCGCY 3 cut(s) 156, 162, 173
HaeIII GGCC 4 cut(s) 75, 218, 636, 654
HapII CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
HgaI GACGC 2 cut(s) 151, 618
HhaI GCGC 6 cut(s) 80, 128, 155, 161, 172, 602
Hin1I GRCGYC 5 cut(s) 143, 153, 159, 170, 610
Hin1II CATG 3 cut(s) 19, 199, 737
Hin6I GCGC 6 cut(s) 78, 126, 153, 159, 170, 600
HinP1I GCGC 6 cut(s) 78, 126, 153, 159, 170, 600
HinfI GANTC 1 cut(s) 176
HpaII CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
HphI GGTGA 2 cut(s) 347, 698
Hpy166II GTNNAC 4 cut(s) 180, 515, 584, 662
Hpy188I TCNGA 2 cut(s) 205, 554
Hpy188III TCNNGA 4 cut(s) 37, 284, 408, 710
Hpy8I GTNNAC 4 cut(s) 180, 515, 584, 662
Hpy99I CGWCG 2 cut(s) 29, 145
HpyAV CCTTC 2 cut(s) 528, 642
HpyCH4IV ACGT 3 cut(s) 182, 346, 664
HpyCH4V TGCA 7 cut(s) 358, 503, 515, 539, 737, 743, 754
HpyF3I CTNAG 1 cut(s) 717
HpySE526I ACGT 3 cut(s) 182, 346, 664
Hsp92I GRCGYC 5 cut(s) 143, 153, 159, 170, 610
Hsp92II CATG 3 cut(s) 19, 199, 737
HspAI GCGC 6 cut(s) 78, 126, 153, 159, 170, 600
KasI GGCGCC 3 cut(s) 152, 158, 169
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 6 cut(s) 4, 56, 205, 377, 483, 706
LmnI GCTCC 4 cut(s) 212, 316, 569, 577
Lsp1109I GCAGC 4 cut(s) 73, 199, 236, 523
LweI GCATC 2 cut(s) 409, 703
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 1 cut(s) 645
MaeII ACGT 3 cut(s) 182, 346, 664
MaeIII GTNAC 1 cut(s) 335
MalI GATC 6 cut(s) 6, 58, 207, 379, 485, 708
MbiI CCGCTC 1 cut(s) 215
MboI GATC 6 cut(s) 4, 56, 205, 377, 483, 706
MboII GAAGA 4 cut(s) 423, 487, 681, 716
MflI RGATCY 2 cut(s) 56, 706
MhlI GDGCHC 2 cut(s) 517, 574
MluCI AATT 1 cut(s) 556
Mly113I GGCGCC 3 cut(s) 153, 159, 170
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 324
MreI CGCCGGCG 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 155
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 72, 536
MspI CCGG 8 cut(s) 54, 60, 82, 93, 99, 129, 156, 173
MspR9I CCNGG 5 cut(s) 54, 82, 83, 99, 510
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 3 cut(s) 72, 78, 126
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 3 cut(s) 153, 159, 170
NciI CCSGG 4 cut(s) 54, 82, 83, 99
NdeII GATC 6 cut(s) 4, 56, 205, 377, 483, 706
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 3 cut(s) 19, 199, 737
NlaIV GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 653
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 335
NotI GCGGCCGC 1 cut(s) 73
NspI RCATGY 2 cut(s) 19, 199
PciI ACATGT 2 cut(s) 15, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 97
PleI GAGTC 1 cut(s) 184
PluTI GGCGCC 3 cut(s) 156, 162, 173
PmaCI CACGTG 1 cut(s) 183
PmlI CACGTG 1 cut(s) 183
PpsI GAGTC 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 2 cut(s) 15, 195
Psp124BI GAGCTC 1 cut(s) 574
Psp1406I AACGTT 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 508
PspCI CACGTG 1 cut(s) 183
PspEI GGTNACC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 8 cut(s) 58, 97, 103, 154, 160, 171, 262, 653
PspPI GGNCC 4 cut(s) 95, 101, 641, 652
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 2 cut(s) 56, 706
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
SacI GAGCTC 1 cut(s) 574
SacII CCGCGG 1 cut(s) 73
SaqAI TTAA 1 cut(s) 435
Sau3AI GATC 6 cut(s) 4, 56, 205, 377, 483, 706
Sau96I GGNCC 4 cut(s) 95, 101, 641, 652
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 5 cut(s) 54, 82, 83, 99, 510
SduI GDGCHC 2 cut(s) 517, 574
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 2 cut(s) 409, 703
SfcI CTRYAG 1 cut(s) 741
SfoI GGCGCC 3 cut(s) 154, 160, 171
Sfr303I CCGCGG 1 cut(s) 73
SgrAI CRCCGGYG 1 cut(s) 155
SgrBI CCGCGG 1 cut(s) 73
SinI GGWCC 3 cut(s) 95, 101, 641
SmaI CCCGGG 1 cut(s) 83
SrfI GCCCGGGC 1 cut(s) 83
Sse9I AATT 1 cut(s) 556
SspDI GGCGCC 3 cut(s) 152, 158, 169
SspMI CTAG 1 cut(s) 645
SstI GAGCTC 1 cut(s) 574
StyD4I CCNGG 5 cut(s) 52, 80, 81, 97, 508
StyI CCWWGG 1 cut(s) 165
TaiI ACGT 3 cut(s) 185, 349, 667
TaqI TCGA 3 cut(s) 24, 252, 285
TasI AATT 1 cut(s) 556
TauI GCSGC 7 cut(s) 72, 75, 78, 114, 164, 218, 431
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 86, 187, 224, 536
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 488
TspMI CCCGGG 1 cut(s) 81
VneI GTGCAC 1 cut(s) 513
VpaK11BI GGWCC 3 cut(s) 95, 101, 641
XapI RAATTY 1 cut(s) 556
XceI RCATGY 2 cut(s) 19, 199
XmaI CCCGGG 1 cut(s) 81
XspI CTAG 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.