MD03G1117900.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003494
Physical Location & Seq
Reverse (-)
10614006 .. 10615987
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1117900.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCAAAAGATATTGAGGGAGCTGAGCACGGTGAGTTTGCCACCAAAGACTACCAGGACCCACCTCCAACCCCCTTGTTTGATGCTGAAGAGCTCACCAAGTGGTCGTTTTACAGAGCTGTCATTGCTGAGTTCGTTGCCACCCTTCTCTTCCTTTACATCACGGTTTTGACTGTGATTGGTTACAAAAGCCAGAGTGAAGTTGACCAATGCGGCGGAGTTGGCATTCTTGGTATCGCCTGGGCCTTCGGTGGCATGATCTTCGTCCTTGTTTACTGCACCGCCGGTATCTCAGGAGGACACATTAACCCAGCTGTGACCTTTGGGCTGTTCTTGGCTCGCAAGGTTTCACTGATCAGGGCAGTGTTGTACATCGTAGCACAGTGTTTGGGTGCCATCTGCGGTGTTGGGTTGGTGAAGGCCTTCCAGAAGTCATACTATGTGAAGTATGGTGGTGGAGCCAACGAGTTGGCTGATGGCTACAACAAGGGCACTGGTTTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTCTACACCGTCTTCTCAGCCACCGACCCCAAGAGAAATGCCAGAGACTCTCACGTCCCTGTTTTGGCACCACTTCCCATTGGATTTGCTGTGTTCATTGTTCACTTGGCCACCATTCCAATCACTGGGACTGGTATCAACCCCGCTAGGAGTTTTGGAGCTGCTGTCATCTACAACAAGGACAAAGCTTGGGATGACCAATGGATCTTCTGGCTTGGACCTTTCATTGGAGCTGCCATTGCTGCCTTCTACCACCAATACATTCTGAGAGCAGGAGCCATCAAGGCTCTAGGATCTTTCAGGAGCAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

282

Amino Acids

30.17

Weight (kDa)

7.65

Isoelectric Point (pI)

29.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 262 2.7e-86 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 584
Acc65I GGTACC 1 cut(s) 518
AccB1I GGYRCC 3 cut(s) 392, 518, 598
AccB7I CCANNNNNTGG 1 cut(s) 656
AccI GTMKAC 1 cut(s) 534
AciI CCGC 5 cut(s) 213, 216, 282, 402, 675
AclWI GGATC 2 cut(s) 743, 832
AcoI YGGCCR 1 cut(s) 639
AcuI CTGAAG 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 102
AfaI GTAC 2 cut(s) 371, 520
AfiI CCNNNNNNNGG 3 cut(s) 595, 656, 758
AjiI CACGTC 1 cut(s) 586
AjnI CCWGG 2 cut(s) 54, 239
AluBI AGCT 7 cut(s) 23, 94, 119, 314, 692, 719, 764
AluI AGCT 7 cut(s) 23, 94, 119, 314, 692, 719, 764
Alw21I GWGCWC 2 cut(s) 30, 96
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 2 cut(s) 743, 832
AoxI GGCC 3 cut(s) 243, 420, 639
ApeKI GCWGC 3 cut(s) 692, 764, 773
Asp700I GAANNNNTTC 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 518
AspS9I GGNCC 3 cut(s) 58, 243, 749
AsuHPI GGTGA 3 cut(s) 44, 88, 427
AvaII GGWCC 2 cut(s) 58, 749
BaeGI GKGCMC 1 cut(s) 494
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 3 cut(s) 392, 518, 598
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 30, 96
BbvI GCAGC 3 cut(s) 679, 751, 760
BccI CCATC 3 cut(s) 404, 470, 818
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 2 cut(s) 56, 241
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 2 cut(s) 678, 821
BglI GCCNNNNNGGC 1 cut(s) 815
BisI GCNGC 4 cut(s) 214, 693, 765, 774
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 4 cut(s) 215, 694, 766, 775
Bme1390I CCNGG 2 cut(s) 56, 241
Bme18I GGWCC 2 cut(s) 58, 749
BmgBI CACGTC 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 58, 243, 749
BmiI GGNNCC 6 cut(s) 60, 394, 460, 520, 600, 808
BmrFI CCNGG 2 cut(s) 56, 241
BmrI ACTGGG 1 cut(s) 666
BmsI GCATC 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 666
BpiI GAAGAC 1 cut(s) 535
Bpu1102I GCTNAGC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 3 cut(s) 595, 656, 758
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 3 cut(s) 499, 661, 667
Bse3DI GCAATG 3 cut(s) 123, 768, 844
BseBI CCWGG 2 cut(s) 56, 241
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 730
BseLI CCNNNNNNNGG 3 cut(s) 595, 656, 758
BseMI GCAATG 3 cut(s) 123, 768, 844
BseMII CTCAG 6 cut(s) 15, 120, 306, 498, 561, 788
BseNI ACTGG 3 cut(s) 499, 661, 667
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 3 cut(s) 679, 751, 760
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 3 cut(s) 245, 422, 641
BshNI GGYRCC 3 cut(s) 392, 518, 598
BsiHKAI GWGCWC 2 cut(s) 30, 96
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 2 cut(s) 572, 673
BslI CCNNNNNNNGG 3 cut(s) 595, 656, 758
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 2 cut(s) 572, 673
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 3 cut(s) 245, 422, 641
Bsp1286I GDGCHC 3 cut(s) 30, 96, 494
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 5 cut(s) 258, 354, 510, 735, 824
Bsp1720I GCTNAGC 1 cut(s) 24
BspACI CCGC 5 cut(s) 213, 216, 282, 402, 675
BspANI GGCC 3 cut(s) 245, 422, 641
BspCNI CTCAG 6 cut(s) 16, 121, 305, 499, 560, 789
BspLI GGNNCC 6 cut(s) 60, 394, 460, 520, 600, 808
BspPI GGATC 2 cut(s) 743, 832
BspQI GCTCTTC 1 cut(s) 84
BspT107I GGYRCC 3 cut(s) 392, 518, 598
BsrDI GCAATG 3 cut(s) 123, 768, 844
BsrFI RCCGGY 1 cut(s) 284
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 3 cut(s) 499, 661, 667
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 5 cut(s) 258, 354, 510, 735, 824
Bst2UI CCWGG 2 cut(s) 56, 241
Bst4CI ACNGT 5 cut(s) 32, 166, 175, 384, 541
Bst6I CTCTTC 2 cut(s) 84, 155
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 6 cut(s) 24, 129, 292, 507, 547, 797
BstF5I GGATG 1 cut(s) 730
BstKTI GATC 5 cut(s) 261, 357, 513, 738, 827
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 5 cut(s) 258, 354, 510, 735, 824
BstMWI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
BstNI CCWGG 2 cut(s) 56, 241
BstSCI CCNGG 2 cut(s) 54, 239
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 3 cut(s) 679, 751, 760
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 2 cut(s) 735, 824
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 2 cut(s) 735, 824
BsuRI GGCC 3 cut(s) 245, 422, 641
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 730
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 5 cut(s) 350, 369, 389, 492, 654
Cac8I GCNNGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 58, 243, 749
Csp6I GTAC 2 cut(s) 370, 519
CviAII CATG 1 cut(s) 256
CviQI GTAC 2 cut(s) 370, 519
DdeI CTNAG 6 cut(s) 24, 129, 292, 507, 547, 797
DpnI GATC 5 cut(s) 260, 356, 512, 737, 826
DpnII GATC 5 cut(s) 258, 354, 510, 735, 824
DraIII CACNNNGTG 1 cut(s) 102
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 2 cut(s) 84, 155
EarI CTCTTC 2 cut(s) 84, 155
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 58, 749
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 58
EcoRII CCWGG 2 cut(s) 54, 239
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 1 cut(s) 259
FaiI YATR 4 cut(s) 257, 436, 441, 450
FaqI GGGAC 2 cut(s) 572, 673
FatI CATG 1 cut(s) 255
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 4 cut(s) 214, 693, 765, 774
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 4 cut(s) 214, 693, 765, 774
FspBI CTAG 2 cut(s) 678, 821
GluI GCNGC 4 cut(s) 214, 693, 765, 774
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 3 cut(s) 245, 422, 641
HapII CCGG 1 cut(s) 285
Hin1II CATG 1 cut(s) 259
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 1 cut(s) 578
HpaII CCGG 1 cut(s) 285
HphI GGTGA 3 cut(s) 44, 88, 427
Hpy166II GTNNAC 4 cut(s) 205, 274, 535, 634
Hpy188I TCNGA 1 cut(s) 798
Hpy188III TCNNGA 3 cut(s) 294, 427, 832
Hpy8I GTNNAC 4 cut(s) 205, 274, 535, 634
HpyAV CCTTC 5 cut(s) 155, 256, 412, 433, 787
HpyCH4III ACNGT 5 cut(s) 32, 166, 175, 384, 541
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
HpyF3I CTNAG 6 cut(s) 24, 129, 292, 507, 547, 797
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 1 cut(s) 259
KpnI GGTACC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 5 cut(s) 258, 354, 510, 735, 824
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 6 cut(s) 20, 458, 689, 761, 806, 834
Lsp1109I GCAGC 3 cut(s) 679, 751, 760
LweI GCATC 1 cut(s) 73
MaeI CTAG 2 cut(s) 678, 821
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 182, 316
MalI GATC 5 cut(s) 260, 356, 512, 737, 826
MboI GATC 5 cut(s) 258, 354, 510, 735, 824
MboII GAAGA 5 cut(s) 101, 142, 253, 535, 730
MflI RGATCY 2 cut(s) 735, 824
MhlI GDGCHC 3 cut(s) 30, 96, 494
MlsI TGGCCA 1 cut(s) 641
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 3 cut(s) 10, 75, 290
Mox20I TGGCCA 1 cut(s) 641
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 1 cut(s) 641
MseI TTAA 2 cut(s) 306, 844
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 2 cut(s) 56, 241
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 2 cut(s) 56, 241
MwoI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
NdeII GATC 5 cut(s) 258, 354, 510, 735, 824
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 6 cut(s) 60, 394, 460, 520, 600, 808
NmuCI GTSAC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 422
PciSI GCTCTTC 1 cut(s) 84
PctI GAATGC 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 422
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 4 cut(s) 215, 694, 766, 775
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PpuMI RGGWCCY 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 54, 239
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 2 cut(s) 54, 239
PspN4I GGNNCC 6 cut(s) 60, 394, 460, 520, 600, 808
PspPI GGNCC 3 cut(s) 58, 243, 749
PspPPI RGGWCCY 1 cut(s) 58
PsuI RGATCY 2 cut(s) 735, 824
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 2 cut(s) 371, 520
RsaNI GTAC 2 cut(s) 370, 519
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 306, 844
SatI GCNGC 4 cut(s) 214, 693, 765, 774
Sau3AI GATC 5 cut(s) 258, 354, 510, 735, 824
Sau96I GGNCC 3 cut(s) 58, 243, 749
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 56, 241
SduI GDGCHC 3 cut(s) 30, 96, 494
SfaNI GCATC 1 cut(s) 73
SinI GGWCC 2 cut(s) 58, 749
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 5 cut(s) 213, 216, 282, 402, 675
SspMI CTAG 2 cut(s) 678, 821
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 2 cut(s) 54, 239
TaaI ACNGT 5 cut(s) 32, 166, 175, 384, 541
TaiI ACGT 1 cut(s) 588
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 216
Tru1I TTAA 2 cut(s) 306, 844
Tru9I TTAA 2 cut(s) 306, 844
TscAI CASTG 5 cut(s) 357, 369, 389, 499, 661
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 692, 764, 773
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 616, 745
TspRI CASTG 5 cut(s) 357, 369, 389, 499, 661
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 58, 749
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 2 cut(s) 678, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.