Rw5G036290

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003494
Physical Location & Seq
Forward (+)
63740221 .. 63742651
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036290.1

Sequence Viewer

Length: 846 bp
ATGGCGAAAGATGTTGAGGGAGCTGAGCACAGTGAGTTTGCGGCAAAGGACTACCATGACCCTCCTCCTACTCCTCTTTTTGATGCCGAGGAGCTGACCAAATGGTCATTTTACAGAGCTCTCATCGCAGAGTTCATTGCTACGCTTCTGTTCCTTTACATCACGGTTTTGACTGTGATTGGGTACAAAGAACAGAGCAAACTCGACCAGTGCGGCGGTGTTGGGATTCTCGGTATCGCTTGGGCCTTCGGTGGCATGATCTTCGTCCTTGTTTACTGCACCGCCGGTATCTCCGGAGGACACATCAACCCAGCTGTGACTTTCGGGCTCTTCTTGGCACGCAAGGTGTCGCTCCTCAGGGCTGTGCTCTACATAGTTGCACAGTGTCTGGGTGCTATCAGTGGTGTAGGGTTGGTCAAGGCCTGGCAGAAGACCTTGTACGTCGAGCATGGTGGTGGAGCCAATGAGTTGTCCCCTGGGTACAGCAAGGGCATCGGATTGGCGGCTGAGATCATCGGCACCTTTGTTCTTGTATACACTGTTTTCTCTGCCACTGACCCTAAGAGAAACGCCAGAGACTCCCATGTTCCTGTCTTGGCACCACTTCCCATTGGATTTGCTGTTTTCATGGTTCACTTGGCCACCATTCCCATTACTGGCACTGGTATCAACCCTGCTAGAAGTTTTGGAGCTGCTGTCATCTACAACCAAGACAAAGCCTGGGATGACCAATGGATGTTCTGGGTTGGACCGTTCATTGGAGCTGCAATTGCTGCCTTTTACCACCAATTTGTTCTGAGAGCAGGAGCAATTAAGGCCCTAGGGTCTTTCAGGAGCAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

281

Amino Acids

30.19

Weight (kDa)

7.05

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 262 8.9e-85 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
AccB1I GGYRCC 2 cut(s) 518, 598
AccI GTMKAC 1 cut(s) 534
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 5 cut(s) 41, 213, 216, 282, 503
AcoI YGGCCR 1 cut(s) 639
AfaI GTAC 3 cut(s) 185, 440, 482
AfiI CCNNNNNNNGG 2 cut(s) 656, 758
AjnI CCWGG 3 cut(s) 422, 475, 719
AluBI AGCT 6 cut(s) 23, 94, 119, 314, 692, 764
AluI AGCT 6 cut(s) 23, 94, 119, 314, 692, 764
Alw21I GWGCWC 3 cut(s) 30, 121, 369
Alw26I GTCTC 1 cut(s) 570
AlwNI CAGNNNCTG 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 4 cut(s) 243, 420, 639, 816
ApeKI GCWGC 3 cut(s) 692, 764, 773
AspA2I CCTAGG 1 cut(s) 820
AspS9I GGNCC 3 cut(s) 243, 749, 817
AvaII GGWCC 1 cut(s) 749
AvrII CCTAGG 1 cut(s) 820
AxyI CCTNAGG 1 cut(s) 356
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 2 cut(s) 518, 598
BanII GRGCYC 2 cut(s) 121, 330
BarI GAAGNNNNNNTAC 2 cut(s) 422, 454
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 3 cut(s) 30, 121, 369
BbvI GCAGC 3 cut(s) 679, 751, 760
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 475, 509
BciT130I CCWGG 3 cut(s) 424, 477, 721
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 2 cut(s) 678, 821
BisI GCNGC 6 cut(s) 42, 214, 504, 693, 765, 774
BlnI CCTAGG 1 cut(s) 820
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 6 cut(s) 43, 215, 505, 694, 766, 775
Bme1390I CCNGG 3 cut(s) 424, 477, 721
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 3 cut(s) 243, 749, 817
BmiI GGNNCC 3 cut(s) 460, 520, 600
BmrFI CCNGG 3 cut(s) 424, 477, 721
BmsI GCATC 2 cut(s) 73, 501
BpiI GAAGAC 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 24
BsaJI CCNNGG 5 cut(s) 87, 475, 476, 720, 820
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 2 cut(s) 656, 758
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 3 cut(s) 208, 661, 667
Bse21I CCTNAGG 1 cut(s) 356
Bse3DI GCAATG 2 cut(s) 135, 844
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 3 cut(s) 424, 477, 721
BseDI CCNNGG 5 cut(s) 87, 475, 476, 720, 820
BseGI GGATG 2 cut(s) 730, 741
BseLI CCNNNNNNNGG 2 cut(s) 656, 758
BseMI GCAATG 2 cut(s) 135, 844
BseMII CTCAG 4 cut(s) 15, 370, 498, 788
BseNI ACTGG 3 cut(s) 208, 661, 667
BseRI GAGGAG 4 cut(s) 54, 63, 104, 344
BseXI GCAGC 3 cut(s) 679, 751, 760
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 4 cut(s) 245, 422, 641, 818
BshNI GGYRCC 2 cut(s) 518, 598
BsiHKAI GWGCWC 3 cut(s) 30, 121, 369
BsiSI CCGG 2 cut(s) 285, 294
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 656, 758
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 4 cut(s) 245, 422, 641, 818
Bsp1286I GDGCHC 4 cut(s) 30, 121, 330, 369
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 2 cut(s) 258, 510
Bsp1720I GCTNAGC 1 cut(s) 24
BspACI CCGC 5 cut(s) 41, 213, 216, 282, 503
BspANI GGCC 4 cut(s) 245, 422, 641, 818
BspCNI CTCAG 4 cut(s) 16, 369, 499, 789
BspEI TCCGGA 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 460, 520, 600
BspQI GCTCTTC 1 cut(s) 335
BspT107I GGYRCC 2 cut(s) 518, 598
BsrDI GCAATG 2 cut(s) 135, 844
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 3 cut(s) 208, 661, 667
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 5 cut(s) 87, 475, 476, 720, 820
BssMI GATC 2 cut(s) 258, 510
BssNAI GTATAC 1 cut(s) 535
BssT1I CCWWGG 1 cut(s) 820
Bst1107I GTATAC 1 cut(s) 535
Bst2UI CCWGG 3 cut(s) 424, 477, 721
Bst4CI ACNGT 6 cut(s) 32, 166, 175, 384, 541, 753
Bst6I CTCTTC 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 5 cut(s) 24, 356, 507, 561, 797
BstF5I GGATG 2 cut(s) 730, 741
BstKTI GATC 2 cut(s) 261, 513
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 2 cut(s) 258, 510
BstMWI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
BstNI CCWGG 3 cut(s) 424, 477, 721
BstSCI CCNGG 3 cut(s) 422, 475, 719
BstV1I GCAGC 3 cut(s) 679, 751, 760
BstV2I GAAGAC 1 cut(s) 437
BstZ17I GTATAC 1 cut(s) 535
Bsu36I CCTNAGG 1 cut(s) 356
BsuRI GGCC 4 cut(s) 245, 422, 641, 818
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 2 cut(s) 730, 741
BtsIMutI CAGTG 7 cut(s) 37, 215, 389, 406, 537, 552, 660
Cac8I GCNNGC 1 cut(s) 340
CaiI CAGNNNCTG 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 243, 749, 817
Csp6I GTAC 3 cut(s) 184, 439, 481
CviAII CATG 5 cut(s) 56, 256, 449, 584, 628
CviQI GTAC 3 cut(s) 184, 439, 481
DdeI CTNAG 5 cut(s) 24, 356, 507, 561, 797
DpnI GATC 2 cut(s) 260, 512
DpnII GATC 2 cut(s) 258, 510
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 820
Eco147I AGGCCT 1 cut(s) 422
Eco24I GRGCYC 2 cut(s) 121, 330
Eco47I GGWCC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 119
Eco81I CCTNAGG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 422, 475, 719
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 2 cut(s) 121, 330
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 5 cut(s) 59, 259, 452, 587, 631
FaiI YATR 7 cut(s) 57, 257, 374, 450, 535, 585, 629
FaqI GGGAC 1 cut(s) 457
FatI CATG 5 cut(s) 55, 255, 448, 583, 627
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 6 cut(s) 42, 214, 504, 693, 765, 774
FokI GGATG 2 cut(s) 737, 748
FriOI GRGCYC 2 cut(s) 121, 330
Fsp4HI GCNGC 6 cut(s) 42, 214, 504, 693, 765, 774
FspBI CTAG 2 cut(s) 678, 821
GluI GCNGC 6 cut(s) 42, 214, 504, 693, 765, 774
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 4 cut(s) 245, 422, 641, 818
HapII CCGG 2 cut(s) 285, 294
Hin1II CATG 5 cut(s) 59, 259, 452, 587, 631
HinfI GANTC 2 cut(s) 226, 578
HpaII CCGG 2 cut(s) 285, 294
Hpy166II GTNNAC 3 cut(s) 274, 535, 634
Hpy188I TCNGA 2 cut(s) 497, 798
Hpy188III TCNNGA 2 cut(s) 294, 832
Hpy8I GTNNAC 3 cut(s) 274, 535, 634
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 6 cut(s) 32, 166, 175, 384, 541, 753
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 3 cut(s) 279, 380, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
HpyF3I CTNAG 5 cut(s) 24, 356, 507, 561, 797
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 5 cut(s) 59, 259, 452, 587, 631
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 2 cut(s) 258, 510
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 8 cut(s) 20, 91, 357, 458, 689, 761, 806, 834
Lsp1109I GCAGC 3 cut(s) 679, 751, 760
LweI GCATC 2 cut(s) 73, 501
MaeI CTAG 2 cut(s) 678, 821
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 316
MalI GATC 2 cut(s) 260, 512
MboI GATC 2 cut(s) 258, 510
MboII GAAGA 3 cut(s) 253, 322, 442
MfeI CAATTG 1 cut(s) 768
MhlI GDGCHC 4 cut(s) 30, 121, 330, 369
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 3 cut(s) 768, 788, 810
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 727
MnlI CCTC 7 cut(s) 10, 72, 75, 82, 84, 290, 365
Mox20I TGGCCA 1 cut(s) 641
MroI TCCGGA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 641
MseI TTAA 2 cut(s) 813, 844
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 2 cut(s) 285, 294
MspR9I CCNGG 3 cut(s) 424, 477, 721
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 3 cut(s) 424, 477, 721
MwoI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
NdeII GATC 2 cut(s) 258, 510
NlaIII CATG 5 cut(s) 59, 259, 452, 587, 631
NlaIV GGNNCC 3 cut(s) 460, 520, 600
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 316
PasI CCCWGGG 1 cut(s) 476
PceI AGGCCT 1 cut(s) 422
PciSI GCTCTTC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 6 cut(s) 43, 215, 505, 694, 766, 775
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 121
Psp6I CCWGG 3 cut(s) 422, 475, 719
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 3 cut(s) 422, 475, 719
PspN4I GGNNCC 3 cut(s) 460, 520, 600
PspPI GGNCC 3 cut(s) 243, 749, 817
PstNI CAGNNNCTG 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 3 cut(s) 185, 440, 482
RsaNI GTAC 3 cut(s) 184, 439, 481
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 2 cut(s) 813, 844
SatI GCNGC 6 cut(s) 42, 214, 504, 693, 765, 774
Sau3AI GATC 2 cut(s) 258, 510
Sau96I GGNCC 3 cut(s) 243, 749, 817
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 424, 477, 721
SduI GDGCHC 4 cut(s) 30, 121, 330, 369
SfaNI GCATC 2 cut(s) 73, 501
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 453
Sse9I AATT 3 cut(s) 768, 788, 810
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 5 cut(s) 41, 213, 216, 282, 503
SspMI CTAG 2 cut(s) 678, 821
SstI GAGCTC 1 cut(s) 121
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 3 cut(s) 422, 475, 719
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 6 cut(s) 32, 166, 175, 384, 541, 753
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 204, 444
TasI AATT 3 cut(s) 768, 788, 810
TauI GCSGC 3 cut(s) 44, 216, 506
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 813, 844
Tru9I TTAA 2 cut(s) 813, 844
TscAI CASTG 7 cut(s) 37, 215, 389, 406, 544, 559, 667
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 692, 764, 773
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 124, 616, 745
TspRI CASTG 7 cut(s) 37, 215, 389, 406, 544, 559, 667
VpaK11BI GGWCC 1 cut(s) 749
XmaJI CCTAGG 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 534
XspI CTAG 2 cut(s) 678, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.