MD11G1136200.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003494
Physical Location & Seq
Reverse (-)
12495991 .. 12497497
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1136200.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCAAAAGATGTAGAGGGAGCTGAACACGGTGAGTTTGCCACCAAAGACTACCATGACCCTCCTCCAACCCCCTTGTTTGATGCTGAGGAGCTCACCAAGTGGTCTTTCTACAGAGCTCTCATTGCTGAGTTCATTGCCACCCTCCTCTTCCTTTACATCACAGTTTTGACCGTCATTGGCTACAAATCCCAGAGCGAGGCTGACCAATGCGGCGGTGTTGGCATTCTTGGTATCGCTTGGGCCTTCGGTGGCATGATTTTCGTCCTTGTTTACTGCACCGCCGGTATCTCAGGAGGACACATTAACCCAGCTGTGACCTTTGGGCTGTTCTTGGCTCGCAAGGTTTCACTGATCAGGGCAGTGTTGTACATCATAGCACAGTGTTTGGGTGCTATCTGCGGTGTAGGGTTGGTGAAGGCCTTCCAGAAGTCATACTACATGAAGTATGGTGGTGGAGCCAACGAGTTGTCTGCTGGCTACAACAAGGGCACTGGTTTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTCTACACCGTCTTCTCCGCCACCGACCCCAAGAGAAATGCCAGAGACTCTCACGTCCCTGTCTTGGCCCCACTTCCCATTGGATTTGCTGTGTTCATTGTTCACTTGGCCACTATTCCCATCACTGGAACTGGCATCAACCCTGCTAGGAGTTTTGGAGCTGCTGTCATCTACAACAAGGACAAAGCTTGGGATGACCAATGGATCTTCTGGCTTGGACCTTTCATTGGAGCTGCCATTGCTGCCTTCTACCACCAATACATTCTGAGAGCAGGAGCCATCAAGGCTCTGGGATCTTTCAGAAGCAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

282

Amino Acids

30.18

Weight (kDa)

8.25

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 262 2.2e-86 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 584
Acc65I GGTACC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 518
AccI GTMKAC 1 cut(s) 534
AciI CCGC 5 cut(s) 213, 216, 282, 402, 549
AclWI GGATC 2 cut(s) 743, 832
AcoI YGGCCR 1 cut(s) 639
AdeI CACNNNGTG 1 cut(s) 102
AfaI GTAC 2 cut(s) 371, 520
AfiI CCNNNNNNNGG 4 cut(s) 199, 595, 656, 758
AjiI CACGTC 1 cut(s) 586
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 7 cut(s) 23, 94, 119, 314, 692, 719, 764
AluI AGCT 7 cut(s) 23, 94, 119, 314, 692, 719, 764
Alw21I GWGCWC 2 cut(s) 96, 121
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 2 cut(s) 743, 832
AoxI GGCC 4 cut(s) 243, 420, 597, 639
ApeKI GCWGC 3 cut(s) 692, 764, 773
Asp700I GAANNNNTTC 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 518
AspS9I GGNCC 3 cut(s) 243, 598, 749
AsuHPI GGTGA 3 cut(s) 44, 88, 427
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 494
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 96, 121
BarI GAAGNNNNNNTAC 2 cut(s) 422, 454
BbsI GAAGAC 1 cut(s) 535
Bbv12I GWGCWC 2 cut(s) 96, 121
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 679, 751, 760
BccI CCATC 2 cut(s) 659, 818
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 455, 489
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 1 cut(s) 678
BfmI CTRYAG 1 cut(s) 112
BglI GCCNNNNNGGC 1 cut(s) 815
BisI GCNGC 4 cut(s) 214, 693, 765, 774
BlsI GCNGC 4 cut(s) 215, 694, 766, 775
Bme18I GGWCC 1 cut(s) 749
BmgBI CACGTC 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 243, 598, 749
BmiI GGNNCC 4 cut(s) 460, 520, 600, 808
BmsI GCATC 2 cut(s) 73, 675
BpiI GAAGAC 1 cut(s) 535
Bpu10I CCTNAGC 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 595, 656, 758
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 3 cut(s) 499, 661, 667
Bse3DI GCAATG 4 cut(s) 123, 135, 768, 844
BseGI GGATG 1 cut(s) 730
BseLI CCNNNNNNNGG 4 cut(s) 199, 595, 656, 758
BseMI GCAATG 4 cut(s) 123, 135, 768, 844
BseMII CTCAG 5 cut(s) 78, 120, 306, 498, 788
BseNI ACTGG 3 cut(s) 499, 661, 667
BseRI GAGGAG 3 cut(s) 54, 104, 137
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 3 cut(s) 679, 751, 760
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 4 cut(s) 245, 422, 599, 641
BshNI GGYRCC 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 96, 121
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 4 cut(s) 199, 595, 656, 758
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 572
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 4 cut(s) 245, 422, 599, 641
Bsp1286I GDGCHC 3 cut(s) 96, 121, 494
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 354, 510, 735, 824
BspACI CCGC 5 cut(s) 213, 216, 282, 402, 549
BspANI GGCC 4 cut(s) 245, 422, 599, 641
BspCNI CTCAG 5 cut(s) 79, 121, 305, 499, 789
BspLI GGNNCC 4 cut(s) 460, 520, 600, 808
BspPI GGATC 2 cut(s) 743, 832
BspT107I GGYRCC 1 cut(s) 518
BsrDI GCAATG 4 cut(s) 123, 135, 768, 844
BsrFI RCCGGY 1 cut(s) 284
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 3 cut(s) 499, 661, 667
BssAI RCCGGY 1 cut(s) 284
BssMI GATC 4 cut(s) 354, 510, 735, 824
Bst4CI ACNGT 5 cut(s) 32, 166, 175, 384, 541
Bst6I CTCTTC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 340, 478
BstDEI CTNAG 5 cut(s) 87, 129, 292, 507, 797
BstF5I GGATG 1 cut(s) 730
BstKTI GATC 4 cut(s) 357, 513, 738, 827
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 4 cut(s) 354, 510, 735, 824
BstMWI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 3 cut(s) 679, 751, 760
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 2 cut(s) 735, 824
BstYI RGATCY 2 cut(s) 735, 824
BsuRI GGCC 4 cut(s) 245, 422, 599, 641
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 730
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 5 cut(s) 350, 369, 389, 492, 654
Cac8I GCNNGC 2 cut(s) 340, 478
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 243, 598, 749
Csp6I GTAC 2 cut(s) 370, 519
CviAII CATG 3 cut(s) 56, 256, 442
CviQI GTAC 2 cut(s) 370, 519
DdeI CTNAG 5 cut(s) 87, 129, 292, 507, 797
DpnI GATC 4 cut(s) 356, 512, 737, 826
DpnII GATC 4 cut(s) 354, 510, 735, 824
DraIII CACNNNGTG 1 cut(s) 102
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 1 cut(s) 538
Ecl136II GAGCTC 2 cut(s) 94, 119
Eco147I AGGCCT 1 cut(s) 422
Eco24I GRGCYC 2 cut(s) 96, 121
Eco47I GGWCC 1 cut(s) 749
Eco53kI GAGCTC 2 cut(s) 94, 119
EcoICRI GAGCTC 2 cut(s) 94, 119
EcoT38I GRGCYC 2 cut(s) 96, 121
FaeI CATG 3 cut(s) 59, 259, 445
FaiI YATR 6 cut(s) 57, 257, 377, 436, 443, 450
FalI AAGNNNNNCTT 1 cut(s) 826
FaqI GGGAC 1 cut(s) 572
FatI CATG 3 cut(s) 55, 255, 441
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 4 cut(s) 214, 693, 765, 774
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 2 cut(s) 96, 121
Fsp4HI GCNGC 4 cut(s) 214, 693, 765, 774
FspBI CTAG 1 cut(s) 678
GluI GCNGC 4 cut(s) 214, 693, 765, 774
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 4 cut(s) 245, 422, 599, 641
HapII CCGG 1 cut(s) 285
Hin1II CATG 3 cut(s) 59, 259, 445
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 1 cut(s) 578
HpaII CCGG 1 cut(s) 285
HphI GGTGA 3 cut(s) 44, 88, 427
Hpy166II GTNNAC 3 cut(s) 274, 535, 634
Hpy188I TCNGA 2 cut(s) 798, 833
Hpy188III TCNNGA 2 cut(s) 294, 427
Hpy8I GTNNAC 3 cut(s) 274, 535, 634
HpyAV CCTTC 4 cut(s) 256, 412, 433, 787
HpyCH4III ACNGT 5 cut(s) 32, 166, 175, 384, 541
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 1 cut(s) 279
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
HpyF3I CTNAG 5 cut(s) 87, 129, 292, 507, 797
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 3 cut(s) 59, 259, 445
KpnI GGTACC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 4 cut(s) 354, 510, 735, 824
LmnI GCTCC 6 cut(s) 20, 91, 458, 689, 761, 806
Lsp1109I GCAGC 3 cut(s) 679, 751, 760
LweI GCATC 2 cut(s) 73, 675
MaeI CTAG 1 cut(s) 678
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 356, 512, 737, 826
MboI GATC 4 cut(s) 354, 510, 735, 824
MboII GAAGA 3 cut(s) 142, 535, 730
MflI RGATCY 2 cut(s) 735, 824
MhlI GDGCHC 3 cut(s) 96, 121, 494
MlsI TGGCCA 1 cut(s) 641
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 8 cut(s) 10, 72, 75, 82, 155, 158, 193, 290
Mox20I TGGCCA 1 cut(s) 641
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 1 cut(s) 641
MseI TTAA 2 cut(s) 306, 844
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 125, 222, 770, 773, 815
NdeII GATC 4 cut(s) 354, 510, 735, 824
NlaIII CATG 3 cut(s) 59, 259, 445
NlaIV GGNNCC 4 cut(s) 460, 520, 600, 808
NmuCI GTSAC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 422
PctI GAATGC 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 422
PkrI GCNGC 4 cut(s) 215, 694, 766, 775
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
Psp124BI GAGCTC 2 cut(s) 96, 121
PspFI CCCAGC 1 cut(s) 310
PspN4I GGNNCC 4 cut(s) 460, 520, 600, 808
PspPI GGNCC 3 cut(s) 243, 598, 749
PsuI RGATCY 2 cut(s) 735, 824
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 2 cut(s) 371, 520
RsaNI GTAC 2 cut(s) 370, 519
SacI GAGCTC 2 cut(s) 96, 121
SaqAI TTAA 2 cut(s) 306, 844
SatI GCNGC 4 cut(s) 214, 693, 765, 774
Sau3AI GATC 4 cut(s) 354, 510, 735, 824
Sau96I GGNCC 3 cut(s) 243, 598, 749
SchI GAGTC 1 cut(s) 572
SduI GDGCHC 3 cut(s) 96, 121, 494
SfaNI GCATC 2 cut(s) 73, 675
SfcI CTRYAG 1 cut(s) 112
SinI GGWCC 1 cut(s) 749
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 5 cut(s) 213, 216, 282, 402, 549
SspMI CTAG 1 cut(s) 678
SstI GAGCTC 2 cut(s) 96, 121
StuI AGGCCT 1 cut(s) 422
TaaI ACNGT 5 cut(s) 32, 166, 175, 384, 541
TaiI ACGT 1 cut(s) 588
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 216
Tru1I TTAA 2 cut(s) 306, 844
Tru9I TTAA 2 cut(s) 306, 844
TscAI CASTG 5 cut(s) 357, 369, 389, 499, 661
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 692, 764, 773
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 124, 458, 616, 745
TspRI CASTG 5 cut(s) 357, 369, 389, 499, 661
VpaK11BI GGWCC 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.