MD03G1128500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009471
Physical Location & Seq
Reverse (-)
12489707 .. 12491694
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1128500.v1.1.491

Sequence Viewer

Length: 306 bp
ATGTTTGTTCTCACTTTGATACTGGGCTGTAATTTTCGGGACAATTTCATGGAAGTGTTCACTTGGAGTATACTGAATGTTTCCTGTGTACTCTCCATGGCCCGATCACGGAATGCAATTGTTGCTACTGGCTTGGTAATTTTCGCAGCTGCAGGCTTGGCATTTCCCTTTTACATGGCGTCATCGCCAAAGCCTGTCATTGACCCATCAAAGCCACTATCACCCCAGGCAACATTTCGAGGGCCTTATATAAACACTGGTTCTCGTGACGTTGGACCTGACTATCAGACCTACCCGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

102

Amino Acids

11.1

Weight (kDa)

9.39

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 108
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
AoxI GGCC 2 cut(s) 99, 242
ApeKI GCWGC 2 cut(s) 146, 149
AspS9I GGNCC 3 cut(s) 100, 242, 275
AsuHPI GGTGA 1 cut(s) 213
AvaII GGWCC 1 cut(s) 275
BauI CACGAG 1 cut(s) 264
BbvI GCAGC 2 cut(s) 136, 158
BccI CCATC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 2 cut(s) 147, 150
BlsI GCNGC 2 cut(s) 148, 151
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 100, 242, 275
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 96, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 3 cut(s) 27, 133, 262
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 96, 225
BseLI CCNNNNNNNGG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 27, 133, 262
BseXI GCAGC 2 cut(s) 136, 158
BshFI GGCC 2 cut(s) 101, 244
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 2 cut(s) 101, 244
Bsp143I GATC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 2 cut(s) 101, 244
BspMAI CTGCAG 1 cut(s) 154
BsrI ACTGG 3 cut(s) 27, 133, 262
BssECI CCNNGG 2 cut(s) 96, 225
BssMI GATC 1 cut(s) 104
BssNAI GTATAC 1 cut(s) 71
BssNI GRCGYC 1 cut(s) 179
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 96
Bst1107I GTATAC 1 cut(s) 71
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 154
BstDSI CCRYGG 1 cut(s) 96
BstKTI GATC 1 cut(s) 107
BstMBI GATC 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 122, 158
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 136, 158
BstZ17I GTATAC 1 cut(s) 71
BsuRI GGCC 2 cut(s) 101, 244
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 100, 242, 275
CseI GACGC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 89
CviAII CATG 3 cut(s) 49, 97, 175
CviJI RGCY 8 cut(s) 27, 101, 132, 149, 156, 193, 214, 244
CviKI_1 RGCY 8 cut(s) 27, 101, 132, 149, 156, 193, 214, 244
CviQI GTAC 1 cut(s) 89
DpnI GATC 1 cut(s) 106
DpnII GATC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 3 cut(s) 52, 100, 178
FaiI YATR 6 cut(s) 50, 71, 98, 176, 249, 251
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 48, 96, 174
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 147, 150
Fsp4HI GCNGC 2 cut(s) 147, 150
GluI GCNGC 2 cut(s) 147, 150
HaeIII GGCC 2 cut(s) 101, 244
HgaI GACGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 3 cut(s) 52, 100, 178
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 3 cut(s) 60, 71, 89
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 2 cut(s) 38, 266
Hpy8I GTNNAC 3 cut(s) 60, 71, 89
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 2 cut(s) 116, 152
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 158
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 3 cut(s) 52, 100, 178
Kzo9I GATC 1 cut(s) 104
LpnPI CCDG 9 cut(s) 8, 97, 114, 138, 207, 212, 239, 243, 291
Lsp1109I GCAGC 2 cut(s) 136, 158
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 266
MalI GATC 1 cut(s) 106
MboI GATC 1 cut(s) 104
MfeI CAATTG 1 cut(s) 117
MluCI AATT 4 cut(s) 31, 43, 117, 138
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 122, 158
NcoI CCATGG 1 cut(s) 96
NdeII GATC 1 cut(s) 104
NlaIII CATG 3 cut(s) 52, 100, 178
NmuCI GTSAC 1 cut(s) 266
PctI GAATGC 1 cut(s) 118
PkrI GCNGC 2 cut(s) 148, 151
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 3 cut(s) 100, 242, 275
PstI CTGCAG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 53
SatI GCNGC 2 cut(s) 147, 150
Sau3AI GATC 1 cut(s) 104
Sau96I GGNCC 3 cut(s) 100, 242, 275
ScrFI CCNGG 1 cut(s) 227
SetI ASST 4 cut(s) 151, 273, 280, 293
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 4 cut(s) 31, 43, 117, 138
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 96
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 238
TasI AATT 4 cut(s) 31, 43, 117, 138
TatI WGTACW 1 cut(s) 88
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 2 cut(s) 146, 149
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.