Rh5CG405400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009471
Physical Location & Seq
Forward (+)
54255778 .. 54259522
3745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG405400.1

Sequence Viewer

Length: 480 bp
ATGTCCAAAGGAAGCCTCATTTTAGCCAAAGACTCGGTAAATTGCTGCATGCTTTTCATGTACATGTCTACAAAATTCTGTTGCACCACATTTTGCCCCGGAACATTGACAGTGACGACAGCTGTTAAGTTCCTTCCTTGCGGGGAGCTAGAGTTGCAAATTTGCGGCCTTTGCACTCCCCCCAGCCTAAACCCAAATCCAAGTCTCCGGCGACCAATCGCCGTCATTTGGAAACTGACTCAGATATATAGTTCTACTTCGGTAGTCTTAATGGCACTATCACGGAATGCTATTGTTGCTACTGGCCTGGTAGTTTTCGCATCTGCGGGGTTGGCATTTCCCTTTTACATGGCGTCATCAAAAACGCCTGTTATCGATCCAACAAAGCCACTGTCACCTCAGGCAACTTTTCGAGGGCCTTACATAAACACTGGTTCGCGTGACGTTGGACCTGATCATCAGACCTACCCAAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.17

Weight (kDa)

9.22

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 68
AccII CGCG 1 cut(s) 439
AciI CCGC 3 cut(s) 141, 165, 326
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 74, 159
AcyI GRCGYC 1 cut(s) 353
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 228
AflIII ACRYGT 1 cut(s) 63
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 2 cut(s) 122, 148
AluI AGCT 2 cut(s) 122, 148
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 371
AoxI GGCC 3 cut(s) 166, 304, 416
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 2 cut(s) 74, 159
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AspS9I GGNCC 2 cut(s) 416, 449
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 387
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 399
BbvI GCAGC 1 cut(s) 32
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 308
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 1 cut(s) 149
BisI GCNGC 2 cut(s) 46, 166
BlsI GCNGC 2 cut(s) 47, 167
Bme1390I CCNGG 2 cut(s) 99, 308
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 416, 449
BmrFI CCNGG 2 cut(s) 99, 308
BmsI GCATC 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 2 cut(s) 307, 436
Bse21I CCTNAGG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 308
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 97
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 254, 413
BseNI ACTGG 2 cut(s) 307, 436
BseXI GCAGC 1 cut(s) 32
BseYI CCCAGC 1 cut(s) 182
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 168, 306, 418
BshVI ATCGAT 1 cut(s) 375
BsiSI CCGG 2 cut(s) 99, 208
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 3 cut(s) 168, 306, 418
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 2 cut(s) 376, 454
BspACI CCGC 3 cut(s) 141, 165, 326
BspANI GGCC 3 cut(s) 168, 306, 418
BspCNI CTCAG 2 cut(s) 253, 412
BspDI ATCGAT 1 cut(s) 375
BspFNI CGCG 1 cut(s) 439
BspPI GGATC 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 2 cut(s) 307, 436
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 376, 454
BssNI GRCGYC 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 112, 393
BstACI GRCGYC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 240, 399
BstFNI CGCG 1 cut(s) 439
BstKTI GATC 2 cut(s) 379, 457
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 2 cut(s) 376, 454
BstMWI GCNNNNNNNGC 4 cut(s) 154, 171, 296, 332
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 2 cut(s) 52, 67
BstSCI CCNGG 2 cut(s) 97, 306
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 375
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 168, 306, 418
BsuTUI ATCGAT 1 cut(s) 375
BtsIMutI CAGTG 3 cut(s) 117, 389, 429
Cac8I GCNNGC 1 cut(s) 50
Cfr13I GGNCC 2 cut(s) 416, 449
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 61
CviAII CATG 4 cut(s) 49, 58, 64, 349
CviJI RGCY 9 cut(s) 15, 26, 122, 148, 168, 186, 306, 388, 418
CviKI_1 RGCY 9 cut(s) 15, 26, 122, 148, 168, 186, 306, 388, 418
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 2 cut(s) 240, 399
DpnI GATC 2 cut(s) 378, 456
DpnII GATC 2 cut(s) 376, 454
Eco47I GGWCC 1 cut(s) 449
Eco81I CCTNAGG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 306
FaeI CATG 4 cut(s) 52, 61, 67, 352
FaiI YATR 7 cut(s) 50, 59, 65, 247, 249, 350, 425
FatI CATG 4 cut(s) 48, 57, 63, 348
FauI CCCGC 2 cut(s) 134, 319
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 46, 166
Fsp4HI GCNGC 2 cut(s) 46, 166
FspBI CTAG 1 cut(s) 149
GluI GCNGC 2 cut(s) 46, 166
GsaI CCCAGC 1 cut(s) 186
HaeIII GGCC 3 cut(s) 168, 306, 418
HapII CCGG 2 cut(s) 99, 208
HgaI GACGC 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 4 cut(s) 52, 61, 67, 352
HinfI GANTC 2 cut(s) 32, 238
HpaII CCGG 2 cut(s) 99, 208
HphI GGTGA 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 243, 462
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 112, 393
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 4 cut(s) 48, 84, 157, 174
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 171, 296, 332
HpyF3I CTNAG 2 cut(s) 240, 399
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 4 cut(s) 52, 61, 67, 352
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 2 cut(s) 376, 454
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 329
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 3 cut(s) 112, 393, 440
MalI GATC 2 cut(s) 378, 456
MboI GATC 2 cut(s) 376, 454
MluCI AATT 3 cut(s) 40, 74, 159
MlyI GAGTC 2 cut(s) 26, 232
MmeI TCCRAC 2 cut(s) 404, 427
MnlI CCTC 3 cut(s) 26, 407, 408
MseI TTAA 2 cut(s) 126, 269
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 2 cut(s) 99, 208
MspR9I CCNGG 2 cut(s) 99, 308
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 4 cut(s) 154, 171, 296, 332
NciI CCSGG 1 cut(s) 99
NdeII GATC 2 cut(s) 376, 454
NlaIII CATG 4 cut(s) 52, 61, 67, 352
NmuCI GTSAC 3 cut(s) 112, 393, 440
NspI RCATGY 2 cut(s) 52, 67
PaeI GCATGC 1 cut(s) 52
PciI ACATGT 1 cut(s) 63
PctI GAATGC 1 cut(s) 292
PkrI GCNGC 2 cut(s) 47, 167
PleI GAGTC 2 cut(s) 26, 232
PpsI GAGTC 2 cut(s) 26, 232
PscI ACATGT 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 306
PspPI GGNCC 2 cut(s) 416, 449
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 2 cut(s) 126, 269
SatI GCNGC 2 cut(s) 46, 166
Sau3AI GATC 2 cut(s) 376, 454
Sau96I GGNCC 2 cut(s) 416, 449
SchI GAGTC 2 cut(s) 26, 232
ScrFI CCNGG 2 cut(s) 99, 308
SetI ASST 6 cut(s) 124, 150, 400, 447, 454, 467
SfaNI GCATC 1 cut(s) 329
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 62
SphI GCATGC 1 cut(s) 52
Sse9I AATT 3 cut(s) 40, 74, 159
SsiI CCGC 3 cut(s) 141, 165, 326
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 97, 306
TaaI ACNGT 2 cut(s) 112, 393
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 375, 412
TasI AATT 3 cut(s) 40, 74, 159
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 126, 269
Tru9I TTAA 2 cut(s) 126, 269
TscAI CASTG 3 cut(s) 117, 396, 436
TseFI GTSAC 3 cut(s) 112, 393, 440
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 3 cut(s) 112, 393, 440
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 3 cut(s) 117, 396, 436
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 2 cut(s) 74, 159
XceI RCATGY 2 cut(s) 52, 67
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.