MD03G1134700.v1.1

phytosulfokines 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004634
Physical Location & Seq
Reverse (-)
13446978 .. 13448023
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1134700.v1.1.491

Sequence Viewer

Length: 285 bp
ATGAAGCAAATCTTCAAATCAGGAGCTGTTCTTGTTCTCTGCATATTTTTCATTCTTTTCTCCTCAACAGTATCGATCCGTCTCTTAGCGAATAAAGAAGGACAAGAGAAGGGGCTACTCAATGAAATGGCTAATGGGGATTCACTTGTAGGACTGGAAGGCACTGAACTACTGGATCTTATGGGGGTGGAGGACTGTGATGATGAAGATGTGGACTGTTCAAAGACAAGGATAATGGCTGAAGCTCACTTGGACTACATCTACACACAGAAACATAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSK

Protein Analysis

95

Amino Acids

10.45

Weight (kDa)

4.61

Isoelectric Point (pI)

24.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 15 - 94 1.2e-15 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 70, 183
AcuI CTGAAG 1 cut(s) 261
AgsI TTSAA 2 cut(s) 16, 222
AluBI AGCT 2 cut(s) 26, 245
AluI AGCT 2 cut(s) 26, 245
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 2 cut(s) 70, 183
AlwNI CAGNNNCTG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 159, 177
BseCI ATCGAT 1 cut(s) 74
BseNI ACTGG 2 cut(s) 159, 177
BseRI GAGGAG 1 cut(s) 52
BshVI ATCGAT 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 86
BsmBI CGTCTC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 75, 175
BspDI ATCGAT 1 cut(s) 74
BspPI GGATC 2 cut(s) 70, 183
BsrI ACTGG 2 cut(s) 159, 177
BssMI GATC 2 cut(s) 75, 175
Bst4CI ACNGT 3 cut(s) 70, 197, 218
BstDEI CTNAG 1 cut(s) 85
BstKTI GATC 2 cut(s) 78, 178
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 75, 175
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
Bsu15I ATCGAT 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 74
BtsIMutI CAGTG 1 cut(s) 162
CaiI CAGNNNCTG 1 cut(s) 26
ClaI ATCGAT 1 cut(s) 74
CviJI RGCY 6 cut(s) 26, 115, 131, 239, 245, 280
CviKI_1 RGCY 6 cut(s) 26, 115, 131, 239, 245, 280
DdeI CTNAG 1 cut(s) 85
DpnI GATC 2 cut(s) 77, 177
DpnII GATC 2 cut(s) 75, 175
Eco57I CTGAAG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 86
FaiI YATR 3 cut(s) 44, 182, 276
FalI AAGNNNNNCTT 1 cut(s) 28
HinfI GANTC 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 21
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 92, 103, 152
HpyCH4III ACNGT 3 cut(s) 70, 197, 218
HpyCH4V TGCA 1 cut(s) 42
HpyF3I CTNAG 1 cut(s) 85
Kzo9I GATC 2 cut(s) 75, 175
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 3 cut(s) 6, 140, 158
MalI GATC 2 cut(s) 77, 177
MboI GATC 2 cut(s) 75, 175
MboII GAAGA 2 cut(s) 4, 218
MflI RGATCY 1 cut(s) 175
MnlI CCTC 2 cut(s) 73, 184
NdeII GATC 2 cut(s) 75, 175
PfeI GAWTC 1 cut(s) 140
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 1 cut(s) 175
Sau3AI GATC 2 cut(s) 75, 175
SetI ASST 2 cut(s) 28, 247
SgeI CNNG 8 cut(s) 33, 44, 116, 158, 167, 185, 240, 262
TaaI ACNGT 3 cut(s) 70, 197, 218
TaqI TCGA 1 cut(s) 74
TfiI GAWTC 1 cut(s) 140
TscAI CASTG 1 cut(s) 169
TspDTI ATGAA 4 cut(s) 17, 40, 138, 219
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.