Rorug05G0292000

phytosulfokines 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004634
Physical Location & Seq
Reverse (-)
33515736 .. 33516227
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0292000.1

Sequence Viewer

Length: 492 bp
ATGCCTGTGTATCAGCATAAGCCCCTTTCTCCGAGCTATATCATCAGTACCACCATCAAGCTGGTTGCTGAGTTTCATGATGCAACAAACCGCTTCCTGGCTACTCCGATCCATGACACCTATAGAACTGATCCTCATCTAATTTGGATTGCTCCCCCACCAGATCATGTCAAAGTCAATTGCGATGTGTCTTGGCTGGCCCCAAATTCAGCCGGACTTGGAGTAGTCATCAGGGATCATACTGGTTCTCTTCTTGGTGGTTCTGCTATCCAAGCAAATTGTTCTTCAGTGGAAATTGCAAAATCTGAACCAATACTATCTGGTGTCAATCTTGCAGTATTAATGAATTTGAAATTGGTTAAGTTTGAATCAGATGCAAGGGAGGTTATCTCTAACCTGAAGAATCCAATTAGCCAGAGCTGGAAATCTTATCCAATCATCGATCAAATAAGGAGACGATGCTCCTACTTTGATTACTACACTTGGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSK

Protein Analysis

163

Amino Acids

18.28

Weight (kDa)

7.0

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 60 - 162 1.2e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 91
AclWI GGATC 3 cut(s) 103, 125, 243
AcsI RAATTY 2 cut(s) 205, 346
AcuI CTGAAG 2 cut(s) 270, 419
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 2 cut(s) 352, 368
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 3 cut(s) 36, 61, 420
AluI AGCT 3 cut(s) 36, 61, 420
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 3 cut(s) 103, 125, 243
AoxI GGCC 1 cut(s) 198
ApoI RAATTY 2 cut(s) 205, 346
AseI ATTAAT 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 199
BccI CCATC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 1 cut(s) 490
BfmI CTRYAG 1 cut(s) 121
Bme1390I CCNGG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 3 cut(s) 70, 364, 449
BplI GAGNNNNNCTC 2 cut(s) 374, 406
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 1 cut(s) 98
BseCI ATCGAT 1 cut(s) 441
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 247
BshFI GGCC 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 441
BsiSI CCGG 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 448
BsmBI CGTCTC 1 cut(s) 448
BsnI GGCC 1 cut(s) 200
Bsp143I GATC 5 cut(s) 108, 130, 163, 235, 442
BspACI CCGC 1 cut(s) 91
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 61
BspDI ATCGAT 1 cut(s) 441
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 3 cut(s) 103, 125, 243
BsrI ACTGG 1 cut(s) 247
BssMI GATC 5 cut(s) 108, 130, 163, 235, 442
Bst2UI CCWGG 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 69
BstKTI GATC 5 cut(s) 111, 133, 166, 238, 445
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 5 cut(s) 108, 130, 163, 235, 442
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 441
BtgZI GCGATG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 198
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 441
Csp6I GTAC 1 cut(s) 48
CviAII CATG 3 cut(s) 77, 113, 167
CviJI RGCY 9 cut(s) 22, 36, 61, 101, 196, 200, 212, 414, 420
CviKI_1 RGCY 9 cut(s) 22, 36, 61, 101, 196, 200, 212, 414, 420
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 69
DpnI GATC 5 cut(s) 110, 132, 165, 237, 444
DpnII GATC 5 cut(s) 108, 130, 163, 235, 442
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco57I CTGAAG 2 cut(s) 270, 419
EcoRII CCWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 448
FaeI CATG 3 cut(s) 80, 116, 170
FaiI YATR 7 cut(s) 18, 39, 78, 114, 123, 168, 240
FatI CATG 3 cut(s) 76, 112, 166
FspBI CTAG 1 cut(s) 490
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 213
Hin1II CATG 3 cut(s) 80, 116, 170
HinfI GANTC 2 cut(s) 368, 403
HpaII CCGG 1 cut(s) 213
Hpy188I TCNGA 4 cut(s) 33, 108, 307, 373
Hpy188III TCNNGA 1 cut(s) 77
HpyCH4V TGCA 4 cut(s) 83, 299, 335, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 3 cut(s) 80, 116, 170
Kzo9I GATC 5 cut(s) 108, 130, 163, 235, 442
LmnI GCTCC 2 cut(s) 157, 467
LweI GCATC 3 cut(s) 70, 364, 449
MaeI CTAG 1 cut(s) 490
MalI GATC 5 cut(s) 110, 132, 165, 237, 444
MboI GATC 5 cut(s) 108, 130, 163, 235, 442
MboII GAAGA 3 cut(s) 242, 276, 412
MfeI CAATTG 1 cut(s) 178
MluCI AATT 8 cut(s) 141, 178, 205, 277, 294, 346, 353, 408
MnlI CCTC 2 cut(s) 144, 376
MseI TTAA 2 cut(s) 341, 360
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeII GATC 5 cut(s) 108, 130, 163, 235, 442
NlaIII CATG 3 cut(s) 80, 116, 170
NlaIV GGNNCC 1 cut(s) 201
PagI TCATGA 1 cut(s) 76
PfeI GAWTC 2 cut(s) 368, 403
PshBI ATTAAT 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 2 cut(s) 341, 360
Sau3AI GATC 5 cut(s) 108, 130, 163, 235, 442
Sau96I GGNCC 1 cut(s) 199
ScrFI CCNGG 1 cut(s) 98
SetI ASST 6 cut(s) 38, 63, 122, 387, 399, 422
SfaNI GCATC 3 cut(s) 70, 364, 449
SfcI CTRYAG 1 cut(s) 121
Sse9I AATT 8 cut(s) 141, 178, 205, 277, 294, 346, 353, 408
SsiI CCGC 1 cut(s) 91
SspMI CTAG 1 cut(s) 490
StyD4I CCNGG 1 cut(s) 96
TaqI TCGA 1 cut(s) 441
TasI AATT 8 cut(s) 141, 178, 205, 277, 294, 346, 353, 408
TfiI GAWTC 2 cut(s) 368, 403
Tru1I TTAA 2 cut(s) 341, 360
Tru9I TTAA 2 cut(s) 341, 360
TscAI CASTG 1 cut(s) 294
TspDTI ATGAA 2 cut(s) 65, 359
TspRI CASTG 1 cut(s) 294
VspI ATTAAT 1 cut(s) 341
XapI RAATTY 2 cut(s) 205, 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XspI CTAG 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.