MD03G1153200.v1.1

Phosphoribosylglycinamide formyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013535
Physical Location & Seq
Reverse (-)
17371372 .. 17375094
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1153200.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGAAGCTCAGCGTCTGCTTTCTGGGTTTTGCTCAACATCGCCGATTCCAAATGCCAAAACCCAATTCTTTGTCAAATATCCCTCCCCTTCTTCTTCCACCACATCTTTTGCTCAATCCCACAAATGGGTCTCCTTCAAAGCTCGCCCTTTGTCTACCAAGTTAATAAAGTGCACGAACACCACACACACAGAGACAGCTGAGGTGGTGGTTTCTTCGGATTCATACAGTCAAGATTCGATAGGTGGGATTCTGCGGAAAAAGCTTGCGGTTTTTGTCTCCGGCGGTGGCTCCAATTTCCGATCAATCCATGAAGCGTGTCTTAACGGTTCAATTCTCGGAGACGTTGTCATTTTGGTCGCAAGTAAACAAGGTTGCGGAGGTGCCGACTATGCAAGAGATAAGAGCCTCCCGGTTATAGTGTTCCCCAAAACTAAACTTGAGCCCGATGGAATATCTCCGGCTGACCTTGTTGCTACCCTTAGGGGATTGGAGGTTGACTTTGTCCTCCTGGCTGGTTACTTGAAACTTATACCGGTAGAGTTGATACAAGCTTATCCCATATCCATACTGAACATCCATCCCTCACTCCTACCAGCATTTGGAGGCAAAGGTAATTACGGTATGAAGGTCCACAAAGCAGTCATTGCTTCTGGGGCAAGATACTCAGGCCCTACAATACATTTTGTTGATGAGCACTATGATACAGGACGTATCCTTGCTCAAAGAGTTGTCCTTGTGCTTGCTAAGGACACTGCCGAGGAGCTGGCAGCAAGGGTTCTTAGGGAGGAGCACGCCTTATATGTGGAAGTAACAAAAGCGTTATGTGAAGAACGGATAATTTGGAGGGAGGACGGTGTTCCTATCATCCGAAGCAAAGAAAATCCTGCTGAATACAGCTAG

Protein Analysis

301

Amino Acids

32.57

Weight (kDa)

8.49

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 87 - 272 2.6e-53 Formyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31220
fragaria_vesca FvH4_5g23990
malus_domestica MD03G1153200.v1.1 MD10G1330400.v1.1
prunus_persica Prupe.4G012000_v2.0.a1
pyrus_communis pycom03g10680 pycom10g27980
rosa_chinensis RchiOBHm_Chr7g0213451
rosa_laevigata RLG00000002803
rosa_multiflora Rmu_sc0013012.1_g000010 Rmu_ssc0000262.1_g000002
rosa_roxburghii Rroxscaffold_3G00245870
rosa_rugosa Rorug07G0142400
rosa_samantha Rh7AG274400 Rh7BG269900 Rh7CG293600 Rh7DG281900
rosa_wichuraiana Rw7G023800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 602
AccI GTMKAC 1 cut(s) 155
AciI CCGC 4 cut(s) 256, 269, 285, 378
AfiI CCNNNNNNNGG 3 cut(s) 126, 127, 602
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 3 cut(s) 139, 333, 526
AjnI CCWGG 1 cut(s) 510
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 7 cut(s) 8, 143, 200, 265, 554, 766, 900
AluI AGCT 7 cut(s) 8, 143, 200, 265, 554, 766, 900
Alw21I GWGCWC 3 cut(s) 176, 699, 795
Alw26I GTCTC 4 cut(s) 136, 188, 283, 336
Alw44I GTGCAC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 16
AoxI GGCC 1 cut(s) 670
ApaLI GTGCAC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 770
AsiGI ACCGGT 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 631, 671
AsuC2I CCSGG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 3 cut(s) 176, 699, 795
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 782
BccI CCATC 2 cut(s) 443, 588
BciT130I CCWGG 1 cut(s) 512
BciVI GTATCC 1 cut(s) 725
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 4 cut(s) 136, 188, 283, 336
BfaI CTAG 1 cut(s) 901
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 1 cut(s) 771
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 772
Bme1390I CCNGG 2 cut(s) 413, 512
Bme18I GGWCC 1 cut(s) 631
BmgT120I GGNCC 2 cut(s) 631, 671
BmiI GGNNCC 2 cut(s) 292, 385
BmrFI CCNGG 2 cut(s) 413, 512
Bpu10I CCTNAGC 2 cut(s) 201, 747
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 413
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 759
BsaWI WCCGGW 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 127, 602
Bse118I RCCGGY 1 cut(s) 535
Bse21I CCTNAGG 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 1 cut(s) 759
BseGI GGATG 3 cut(s) 576, 580, 867
BseLI CCNNNNNNNGG 3 cut(s) 126, 127, 602
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 3 cut(s) 23, 192, 681
BseRI GAGGAG 2 cut(s) 776, 803
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 1 cut(s) 782
BshFI GGCC 1 cut(s) 672
BshNI GGYRCC 1 cut(s) 383
BshTI ACCGGT 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 176, 699, 795
BsiSI CCGG 4 cut(s) 282, 413, 461, 536
BslI CCNNNNNNNGG 3 cut(s) 126, 127, 602
BsmAI GTCTC 4 cut(s) 136, 188, 283, 336
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 1 cut(s) 672
Bso31I GGTCTC 1 cut(s) 136
Bsp1286I GDGCHC 4 cut(s) 176, 447, 699, 795
Bsp143I GATC 1 cut(s) 302
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 4 cut(s) 256, 269, 285, 378
BspANI GGCC 1 cut(s) 672
BspCNI CTCAG 3 cut(s) 22, 193, 680
BspLI GGNNCC 2 cut(s) 292, 385
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 759
BssMI GATC 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 4 cut(s) 230, 329, 623, 857
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 5 cut(s) 145, 267, 744, 768, 795
BstDEI CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
BstF5I GGATG 3 cut(s) 576, 580, 867
BstKTI GATC 1 cut(s) 305
BstMAI GTCTC 4 cut(s) 136, 188, 283, 336
BstMBI GATC 1 cut(s) 302
BstMWI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
BstNI CCWGG 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 411, 510
BstSLI GKGCMC 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 782
Bsu36I CCTNAGG 1 cut(s) 482
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 1 cut(s) 672
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 3 cut(s) 576, 580, 867
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 1 cut(s) 753
Cac8I GCNNGC 5 cut(s) 145, 267, 744, 768, 795
CaiI CAGNNNCTG 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 631, 671
CseI GACGC 1 cut(s) 2
CspAI ACCGGT 1 cut(s) 535
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviAII CATG 1 cut(s) 311
DdeI CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
DpnI GATC 1 cut(s) 304
DpnII GATC 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 631
Eco81I CCTNAGG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 314
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FatI CATG 1 cut(s) 310
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 771
FokI GGATG 3 cut(s) 563, 567, 854
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 771
FspBI CTAG 1 cut(s) 901
GluI GCNGC 1 cut(s) 771
HaeIII GGCC 1 cut(s) 672
HapII CCGG 4 cut(s) 282, 413, 461, 536
HgaI GACGC 1 cut(s) 2
Hin1II CATG 1 cut(s) 314
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 2 cut(s) 263, 552
HinfI GANTC 4 cut(s) 46, 221, 236, 250
HpaII CCGG 4 cut(s) 282, 413, 461, 536
Hpy166II GTNNAC 5 cut(s) 156, 174, 368, 499, 634
Hpy188I TCNGA 4 cut(s) 220, 302, 341, 872
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 5 cut(s) 156, 174, 368, 499, 634
HpyAV CCTTC 3 cut(s) 99, 145, 622
HpyCH4III ACNGT 4 cut(s) 230, 329, 623, 857
HpyCH4IV ACGT 2 cut(s) 345, 712
HpyCH4V TGCA 2 cut(s) 174, 395
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
HpyF3I CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
HpySE526I ACGT 2 cut(s) 345, 712
Hsp92II CATG 1 cut(s) 314
Kzo9I GATC 1 cut(s) 302
LmnI GCTCC 3 cut(s) 296, 763, 790
Lsp1109I GCAGC 1 cut(s) 782
MaeI CTAG 1 cut(s) 901
MaeII ACGT 2 cut(s) 345, 712
MaeIII GTNAC 2 cut(s) 518, 811
MalI GATC 1 cut(s) 304
MboI GATC 1 cut(s) 302
MboII GAAGA 4 cut(s) 84, 87, 207, 842
MhlI GDGCHC 4 cut(s) 176, 447, 699, 795
MluCI AATT 5 cut(s) 65, 295, 333, 616, 840
MseI TTAA 2 cut(s) 164, 324
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 4 cut(s) 282, 413, 461, 536
MspR9I CCNGG 2 cut(s) 413, 512
MvaI CCWGG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
NciI CCSGG 1 cut(s) 413
NdeII GATC 1 cut(s) 302
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 2 cut(s) 292, 385
NmeAIII GCCGAG 1 cut(s) 784
PfeI GAWTC 4 cut(s) 46, 221, 236, 250
PflFI GACNNNGTC 2 cut(s) 347, 503
PflMI CCANNNNNTGG 1 cut(s) 602
PinAI ACCGGT 1 cut(s) 535
PkrI GCNGC 1 cut(s) 772
Psp6I CCWGG 1 cut(s) 510
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 2 cut(s) 292, 385
PspPI GGNCC 2 cut(s) 631, 671
PstNI CAGNNNCTG 1 cut(s) 16
PsyI GACNNNGTC 2 cut(s) 347, 503
PvuII CAGCTG 1 cut(s) 200
SaqAI TTAA 2 cut(s) 164, 324
SatI GCNGC 1 cut(s) 771
Sau3AI GATC 1 cut(s) 302
Sau96I GGNCC 2 cut(s) 631, 671
ScrFI CCNGG 2 cut(s) 413, 512
SduI GDGCHC 4 cut(s) 176, 447, 699, 795
SinI GGWCC 1 cut(s) 631
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 5 cut(s) 65, 295, 333, 616, 840
SsiI CCGC 4 cut(s) 256, 269, 285, 378
SspMI CTAG 1 cut(s) 901
StyD4I CCNGG 2 cut(s) 411, 510
TaaI ACNGT 4 cut(s) 230, 329, 623, 857
TaiI ACGT 2 cut(s) 348, 715
TaqI TCGA 1 cut(s) 239
TasI AATT 5 cut(s) 65, 295, 333, 616, 840
TfiI GAWTC 4 cut(s) 46, 221, 236, 250
Tru1I TTAA 2 cut(s) 164, 324
Tru9I TTAA 2 cut(s) 164, 324
TscAI CASTG 1 cut(s) 760
TseI GCWGC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 213, 327, 641
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 1 cut(s) 760
Tth111I GACNNNGTC 2 cut(s) 347, 503
Van91I CCANNNNNTGG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 155
XspI CTAG 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.