pycom03g10680

Phosphoribosylglycinamide formyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013535
Physical Location & Seq
Reverse (-)
9937306 .. 9939636
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10680.2

Sequence Viewer

Length: 903 bp
ATGGAAGCTCAGCGTCTGCTTTCTGGGTTTTGCTCAACATCGCCGATTCCAAATGCCAAAACCCAATGCTTCGCCAAATTTCCCTCCCCTTCTTCTTCCACCACTTCTTTTGCTCAATCCCACAAATGGGTCTCCTTCAAAGCTCGCCCTTTGTCTACCAAGTTAATAAAGTGCACGAACACCACACACACAGAGACAGCTGAGGTGGTGGTTTCTTCGGATTCATACAGTCAGGATTCGATAAGTGGGATTCCGCGGAAAAAGCTTGCGGTTTTTGTCTCCGGCGGTGGCTCCAATTTCCGGTCAATCCATGAAGCGTGTCTTAACGGTTCGATTCTCGGAGACGTCGTCGTTTTGGTCGCAAGTAAACAAGGTTGCGGAGGTGCCGACTATGCAAGAGATAAGAGCCTCCCGGTTATAGTGTTCCCCAAAACTAAACTTGAGCCCGATGGAATATCTCCGGCTGACCTTGTTGCTACCCTTAGGGGATTGGAGGTTGACTTTGTCCTCCTGGCTGGTTACTTGAAACTTATACCGGTAGAGTTGATACAAGCGTATCCCAGATCCATACTGAACATCCATCCCTCACTCCTACCAGCATTTGGAGGCAAAGGTAATTATGGTATGAAGGTCCACAAAGCAGTCATTGCTTCTGGGGCAAGATACTCAGGCCCTACAATACATTTTGTTGATGAGCACTATGATACAGGACGTATCCTTGCTCAAAGAGTTGTCCCTGTGCTTGCTAAGGACACTGCCGAGGAGCTGGCAGCAAGGGTTCTTAGGGAGGAGCACGTCTTATATGTGGAAGTAACAAAAGCGTTATGTGAAGAACGGATAATTTGGAGGGAGGACGGTGTTCCTATCATCCGAAGCAAAGAAAATCCCGCTGAATACAGCTAG

Protein Analysis

301

Amino Acids

32.54

Weight (kDa)

8.65

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31220
fragaria_vesca FvH4_5g23990
malus_domestica MD03G1153200.v1.1 MD10G1330400.v1.1
prunus_persica Prupe.4G012000_v2.0.a1
pyrus_communis pycom03g10680 pycom10g27980
rosa_chinensis RchiOBHm_Chr7g0213451
rosa_laevigata RLG00000002803
rosa_multiflora Rmu_sc0013012.1_g000010 Rmu_ssc0000262.1_g000002
rosa_roxburghii Rroxscaffold_3G00245870
rosa_rugosa Rorug07G0142400
rosa_samantha Rh7AG274400 Rh7BG269900 Rh7CG293600 Rh7DG281900
rosa_wichuraiana Rw7G023800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 602
AccI GTMKAC 1 cut(s) 155
AccII CGCG 1 cut(s) 256
AciI CCGC 6 cut(s) 254, 256, 269, 285, 378, 888
AclWI GGATC 1 cut(s) 558
AcsI RAATTY 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 345
AfiI CCNNNNNNNGG 4 cut(s) 126, 127, 300, 602
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 2 cut(s) 139, 526
AjiI CACGTC 1 cut(s) 796
AjnI CCWGG 1 cut(s) 510
AluBI AGCT 6 cut(s) 8, 143, 200, 265, 766, 900
AluI AGCT 6 cut(s) 8, 143, 200, 265, 766, 900
Alw21I GWGCWC 3 cut(s) 176, 699, 795
Alw26I GTCTC 4 cut(s) 136, 188, 283, 336
Alw44I GTGCAC 1 cut(s) 172
AlwI GGATC 1 cut(s) 558
AlwNI CAGNNNCTG 1 cut(s) 16
AoxI GGCC 1 cut(s) 670
ApaLI GTGCAC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 77
AsiGI ACCGGT 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 631, 671
AsuC2I CCSGG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 176
BaeI ACNNNNGTAYC 4 cut(s) 539, 539, 572, 572
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 3 cut(s) 176, 699, 795
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 782
BccI CCATC 2 cut(s) 443, 588
BciT130I CCWGG 1 cut(s) 512
BciVI GTATCC 2 cut(s) 567, 725
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 4 cut(s) 136, 188, 283, 336
BfaI CTAG 1 cut(s) 901
BfuI GTATCC 2 cut(s) 567, 725
BisI GCNGC 1 cut(s) 771
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 772
Bme1390I CCNGG 2 cut(s) 413, 512
Bme18I GGWCC 1 cut(s) 631
BmgBI CACGTC 1 cut(s) 796
BmgT120I GGNCC 2 cut(s) 631, 671
BmiI GGNNCC 2 cut(s) 292, 385
BmrFI CCNGG 2 cut(s) 413, 512
Bpu10I CCTNAGC 2 cut(s) 201, 747
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 254, 759
BsaWI WCCGGW 2 cut(s) 300, 535
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 127, 300, 602
Bse118I RCCGGY 1 cut(s) 535
Bse21I CCTNAGG 1 cut(s) 482
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 254, 759
BseGI GGATG 3 cut(s) 576, 580, 867
BseLI CCNNNNNNNGG 4 cut(s) 126, 127, 300, 602
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 3 cut(s) 23, 192, 681
BseRI GAGGAG 2 cut(s) 776, 803
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 1 cut(s) 782
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 1 cut(s) 672
BshNI GGYRCC 1 cut(s) 383
BshTI ACCGGT 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 176, 699, 795
BsiSI CCGG 5 cut(s) 282, 301, 413, 461, 536
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 4 cut(s) 126, 127, 300, 602
BsmAI GTCTC 4 cut(s) 136, 188, 283, 336
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 1 cut(s) 672
Bso31I GGTCTC 1 cut(s) 136
Bsp1286I GDGCHC 4 cut(s) 176, 447, 699, 795
Bsp143I GATC 1 cut(s) 563
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 6 cut(s) 254, 256, 269, 285, 378, 888
BspANI GGCC 1 cut(s) 672
BspCNI CTCAG 3 cut(s) 22, 193, 680
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 292, 385
BspPI GGATC 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 2 cut(s) 254, 759
BssMI GATC 1 cut(s) 563
BssNI GRCGYC 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 3 cut(s) 230, 329, 857
BstACI GRCGYC 1 cut(s) 345
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 4 cut(s) 145, 267, 744, 768
BstDEI CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
BstDSI CCRYGG 1 cut(s) 254
BstF5I GGATG 3 cut(s) 576, 580, 867
BstFNI CGCG 1 cut(s) 256
BstKTI GATC 1 cut(s) 566
BstMAI GTCTC 4 cut(s) 136, 188, 283, 336
BstMBI GATC 1 cut(s) 563
BstMWI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
BstNI CCWGG 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 411, 510
BstSLI GKGCMC 1 cut(s) 176
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 782
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
Bsu36I CCTNAGG 1 cut(s) 482
BsuI GTATCC 2 cut(s) 567, 725
BsuRI GGCC 1 cut(s) 672
BtgI CCRYGG 1 cut(s) 254
BtgZI GCGATG 1 cut(s) 24
BtrI CACGTC 1 cut(s) 796
BtsCI GGATG 3 cut(s) 576, 580, 867
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 1 cut(s) 753
Cac8I GCNNGC 4 cut(s) 145, 267, 744, 768
CaiI CAGNNNCTG 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 631, 671
Cfr42I CCGCGG 1 cut(s) 257
CseI GACGC 1 cut(s) 2
CspAI ACCGGT 1 cut(s) 535
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviAII CATG 1 cut(s) 311
DdeI CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
DpnI GATC 1 cut(s) 565
DpnII GATC 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 631
Eco81I CCTNAGG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 314
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 1 cut(s) 719
FatI CATG 1 cut(s) 310
FauI CCCGC 1 cut(s) 895
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 771
FokI GGATG 3 cut(s) 563, 567, 854
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 771
FspBI CTAG 1 cut(s) 901
GluI GCNGC 1 cut(s) 771
HaeIII GGCC 1 cut(s) 672
HapII CCGG 5 cut(s) 282, 301, 413, 461, 536
HgaI GACGC 1 cut(s) 2
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 1 cut(s) 314
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 5 cut(s) 46, 221, 236, 250, 334
HpaII CCGG 5 cut(s) 282, 301, 413, 461, 536
Hpy166II GTNNAC 5 cut(s) 156, 174, 368, 499, 634
Hpy188I TCNGA 3 cut(s) 220, 341, 872
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 5 cut(s) 156, 174, 368, 499, 634
Hpy99I CGWCG 2 cut(s) 350, 353
HpyAV CCTTC 3 cut(s) 99, 145, 622
HpyCH4III ACNGT 3 cut(s) 230, 329, 857
HpyCH4IV ACGT 3 cut(s) 345, 712, 795
HpyCH4V TGCA 2 cut(s) 174, 395
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
HpyF3I CTNAG 6 cut(s) 9, 201, 482, 667, 747, 782
HpySE526I ACGT 3 cut(s) 345, 712, 795
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 1 cut(s) 314
KspI CCGCGG 1 cut(s) 257
Kzo9I GATC 1 cut(s) 563
LmnI GCTCC 3 cut(s) 296, 763, 790
Lsp1109I GCAGC 1 cut(s) 782
MaeI CTAG 1 cut(s) 901
MaeII ACGT 3 cut(s) 345, 712, 795
MaeIII GTNAC 2 cut(s) 518, 811
MalI GATC 1 cut(s) 565
MboI GATC 1 cut(s) 563
MboII GAAGA 4 cut(s) 84, 87, 207, 842
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 4 cut(s) 176, 447, 699, 795
MluCI AATT 4 cut(s) 77, 295, 616, 840
MseI TTAA 2 cut(s) 164, 324
MspA1I CMGCKG 3 cut(s) 200, 256, 890
MspI CCGG 5 cut(s) 282, 301, 413, 461, 536
MspR9I CCNGG 2 cut(s) 413, 512
MvaI CCWGG 1 cut(s) 512
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 4 cut(s) 262, 392, 647, 656
NciI CCSGG 1 cut(s) 413
NdeII GATC 1 cut(s) 563
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 2 cut(s) 292, 385
NmeAIII GCCGAG 1 cut(s) 784
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PfeI GAWTC 5 cut(s) 46, 221, 236, 250, 334
PflFI GACNNNGTC 2 cut(s) 347, 503
PflMI CCANNNNNTGG 1 cut(s) 602
PinAI ACCGGT 1 cut(s) 535
PkrI GCNGC 1 cut(s) 772
Psp6I CCWGG 1 cut(s) 510
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 2 cut(s) 292, 385
PspPI GGNCC 2 cut(s) 631, 671
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 563
PsyI GACNNNGTC 2 cut(s) 347, 503
PvuII CAGCTG 1 cut(s) 200
SacII CCGCGG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 164, 324
SatI GCNGC 1 cut(s) 771
Sau3AI GATC 1 cut(s) 563
Sau96I GGNCC 2 cut(s) 631, 671
ScrFI CCNGG 2 cut(s) 413, 512
SduI GDGCHC 4 cut(s) 176, 447, 699, 795
Sfr303I CCGCGG 1 cut(s) 257
SgrBI CCGCGG 1 cut(s) 257
SinI GGWCC 1 cut(s) 631
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 4 cut(s) 77, 295, 616, 840
SsiI CCGC 6 cut(s) 254, 256, 269, 285, 378, 888
SspMI CTAG 1 cut(s) 901
StyD4I CCNGG 2 cut(s) 411, 510
TaaI ACNGT 3 cut(s) 230, 329, 857
TaiI ACGT 3 cut(s) 348, 715, 798
TaqI TCGA 2 cut(s) 239, 332
TasI AATT 4 cut(s) 77, 295, 616, 840
TfiI GAWTC 5 cut(s) 46, 221, 236, 250, 334
Tru1I TTAA 2 cut(s) 164, 324
Tru9I TTAA 2 cut(s) 164, 324
TscAI CASTG 1 cut(s) 760
TseI GCWGC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 213, 327, 641
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 1 cut(s) 760
Tth111I GACNNNGTC 2 cut(s) 347, 503
Van91I CCANNNNNTGG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 631
XapI RAATTY 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 155
XspI CTAG 1 cut(s) 901
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.