MD10G1330400.v1.1

Phosphoribosylglycinamide formyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013535
Physical Location & Seq
Forward (+)
40881358 .. 40884179
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1330400.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGAAGCTCAGCGTCTGCTTTCTGGGTTCTGCTCAGCCTCGCCGATTCCAAACGCCAGAACCCAATACTTCGTCAGATTTCCCTCCCCTTCTCCTTCTTCTTCTTCCTCCACCGCTTCTTTTGCTCAATCCCACATGTGGGTCTCCTTCAAAGCTCGCCCTTTGTCTACCAAGTTAATACAGTGCAGGAACACCGCGGACACAGAGAGAGCTGAGGTGATGGTTTCTTCGGATAAACAAGATTTGACAGGTGGGATTCGACGGAAAAAGCTTGCCGTTTTTGTTTCCGGCGGGGGCTCCAACTTCCGTTCGATCCATGAAGCGTGTCTTAACGGTTCAATTCTCGGAGACGTTGTCGTTTTGGTCGCTAGTAAACAAGGTTGCGGAGGTGCCGACTATGCAAGAGAAAAAGGCCTCCCAATTCTGGTGTTCCCGAAAACTAAACTTGAGCCTGACGGAATATCTCCAGCTGACCTTGTTGCTACCCTTAGGGGATTAGAGGTTGACTTTGTTCTCCTGGCTGGTTACTTGAAACTTATACCGGTTGAGTTGATACAAGCTTATCCCAGATCCATACTGAACATCCATCCCTCACTTCTTCCAGCATTTGGAGGCAAAGGTCATTACGGTATGAAGGTCCACAAGGCAGTCATAGCTTCTGGGGCAAGATACACAGGCCCTACAATACATTTTGTCGATGAGCACTATGATACAGGACGTATCCTTGCCCAAAGAGTTGTCCCTGTGCTTGCTAAGGACACTGCCGAGGAGCTGGCAGCAAGGGTTCTTCGGGAGGAGCACTCCTTGTACGTGGAAGTGATAACAGCAGTATGTGAAGAACGGATTGTTTGGAGGGAGGATGGTGTTCCTATTATCCGGAGCAAAGAAAATCCCAACGAATACAGCTAG

Protein Analysis

303

Amino Acids

32.93

Weight (kDa)

8.74

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 89 - 274 9e-55 Formyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31220
fragaria_vesca FvH4_5g23990
malus_domestica MD03G1153200.v1.1 MD10G1330400.v1.1
prunus_persica Prupe.4G012000_v2.0.a1
pyrus_communis pycom03g10680 pycom10g27980
rosa_chinensis RchiOBHm_Chr7g0213451
rosa_laevigata RLG00000002803
rosa_multiflora Rmu_sc0013012.1_g000010 Rmu_ssc0000262.1_g000002
rosa_roxburghii Rroxscaffold_3G00245870
rosa_rugosa Rorug07G0142400
rosa_samantha Rh7AG274400 Rh7BG269900 Rh7CG293600 Rh7DG281900
rosa_wichuraiana Rw7G023800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 389
AccB7I CCANNNNNTGG 1 cut(s) 608
AccI GTMKAC 1 cut(s) 167
AccII CGCG 1 cut(s) 197
AccIII TCCGGA 1 cut(s) 876
AciI CCGC 5 cut(s) 114, 195, 197, 291, 384
AclWI GGATC 2 cut(s) 307, 564
AfaI GTAC 1 cut(s) 809
AfiI CCNNNNNNNGG 4 cut(s) 138, 139, 424, 608
AflIII ACRYGT 1 cut(s) 135
AgeI ACCGGT 1 cut(s) 541
AgsI TTSAA 3 cut(s) 151, 339, 532
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 9 cut(s) 8, 155, 212, 271, 470, 560, 656, 772, 906
AluI AGCT 9 cut(s) 8, 155, 212, 271, 470, 560, 656, 772, 906
Alw21I GWGCWC 2 cut(s) 705, 801
Alw26I GTCTC 2 cut(s) 148, 342
AlwI GGATC 2 cut(s) 307, 564
AlwNI CAGNNNCTG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 876
AoxI GGCC 2 cut(s) 412, 676
ApeKI GCWGC 1 cut(s) 776
AsiGI ACCGGT 1 cut(s) 541
AspS9I GGNCC 2 cut(s) 637, 677
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 637
AxyI CCTNAGG 1 cut(s) 488
BanI GGYRCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 705, 801
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 788
BccI CCATC 3 cut(s) 214, 594, 854
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 518
BciVI GTATCC 1 cut(s) 731
BcoDI GTCTC 2 cut(s) 148, 342
BfaI CTAG 2 cut(s) 369, 907
BfuI GTATCC 1 cut(s) 731
BisI GCNGC 1 cut(s) 777
BlpI GCTNAGC 2 cut(s) 9, 34
BlsI GCNGC 1 cut(s) 778
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 637, 677
BmiI GGNNCC 2 cut(s) 298, 391
BmrFI CCNGG 1 cut(s) 518
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 2 cut(s) 213, 753
Bpu1102I GCTNAGC 2 cut(s) 9, 34
BpuEI CTTGAG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 811
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 195, 765
BsaWI WCCGGW 2 cut(s) 541, 876
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 139, 424, 608
Bse118I RCCGGY 1 cut(s) 541
Bse21I CCTNAGG 1 cut(s) 488
BseAI TCCGGA 1 cut(s) 876
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 195, 765
BseGI GGATG 3 cut(s) 582, 586, 865
BseLI CCNNNNNNNGG 4 cut(s) 138, 139, 424, 608
BseMII CTCAG 3 cut(s) 23, 48, 204
BseRI GAGGAG 2 cut(s) 782, 809
BseXI GCAGC 1 cut(s) 788
BsgI GTGCAG 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 2 cut(s) 414, 678
BshNI GGYRCC 1 cut(s) 389
BshTI ACCGGT 1 cut(s) 541
BsiHKAI GWGCWC 2 cut(s) 705, 801
BsiSI CCGG 3 cut(s) 288, 542, 877
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 4 cut(s) 138, 139, 424, 608
BsmAI GTCTC 2 cut(s) 148, 342
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 2 cut(s) 414, 678
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 3 cut(s) 299, 705, 801
Bsp13I TCCGGA 1 cut(s) 876
Bsp143I GATC 2 cut(s) 312, 569
Bsp1720I GCTNAGC 2 cut(s) 9, 34
BspACI CCGC 5 cut(s) 114, 195, 197, 291, 384
BspANI GGCC 2 cut(s) 414, 678
BspCNI CTCAG 3 cut(s) 22, 47, 205
BspEI TCCGGA 1 cut(s) 876
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 298, 391
BspPI GGATC 2 cut(s) 307, 564
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 541
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 195, 765
BssMI GATC 2 cut(s) 312, 569
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 3 cut(s) 183, 335, 629
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 4 cut(s) 157, 273, 750, 774
BstDEI CTNAG 5 cut(s) 9, 34, 213, 488, 753
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 3 cut(s) 582, 586, 865
BstFNI CGCG 1 cut(s) 197
BstKTI GATC 2 cut(s) 315, 572
BstMAI GTCTC 2 cut(s) 148, 342
BstMBI GATC 2 cut(s) 312, 569
BstMWI GCNNNNNNNGC 4 cut(s) 122, 398, 653, 662
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 516
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 788
BstX2I RGATCY 1 cut(s) 569
BstYI RGATCY 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 488
BsuI GTATCC 1 cut(s) 731
BsuRI GGCC 2 cut(s) 414, 678
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 3 cut(s) 582, 586, 865
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 2 cut(s) 188, 759
Cac8I GCNNGC 4 cut(s) 157, 273, 750, 774
CaiI CAGNNNCTG 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 2 cut(s) 637, 677
Cfr42I CCGCGG 1 cut(s) 198
CseI GACGC 1 cut(s) 2
Csp6I GTAC 1 cut(s) 808
CspAI ACCGGT 1 cut(s) 541
CviAII CATG 2 cut(s) 136, 317
CviQI GTAC 1 cut(s) 808
DdeI CTNAG 5 cut(s) 9, 34, 213, 488, 753
DpnI GATC 2 cut(s) 314, 571
DpnII GATC 2 cut(s) 312, 569
Eco147I AGGCCT 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 637
Eco81I CCTNAGG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 677
EcoRII CCWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 299
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 2 cut(s) 139, 320
FaiI YATR 9 cut(s) 137, 318, 399, 539, 575, 632, 653, 708, 832
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 725
FatI CATG 2 cut(s) 135, 316
FauI CCCGC 1 cut(s) 284
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 1 cut(s) 777
FokI GGATG 3 cut(s) 569, 573, 872
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 777
FspBI CTAG 2 cut(s) 369, 907
GluI GCNGC 1 cut(s) 777
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 414, 678
HapII CCGG 3 cut(s) 288, 542, 877
HgaI GACGC 1 cut(s) 2
Hin1II CATG 2 cut(s) 139, 320
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 2 cut(s) 269, 558
HinfI GANTC 2 cut(s) 46, 256
HpaII CCGG 3 cut(s) 288, 542, 877
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 4 cut(s) 168, 374, 505, 640
Hpy188I TCNGA 3 cut(s) 77, 232, 347
Hpy188III TCNNGA 3 cut(s) 433, 791, 877
Hpy8I GTNNAC 4 cut(s) 168, 374, 505, 640
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 4 cut(s) 99, 105, 157, 628
HpyCH4III ACNGT 3 cut(s) 183, 335, 629
HpyCH4IV ACGT 3 cut(s) 351, 718, 810
HpyCH4V TGCA 2 cut(s) 186, 401
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 398, 653, 662
HpyF3I CTNAG 5 cut(s) 9, 34, 213, 488, 753
HpySE526I ACGT 3 cut(s) 351, 718, 810
Hsp92II CATG 2 cut(s) 139, 320
Kpn2I TCCGGA 1 cut(s) 876
KspI CCGCGG 1 cut(s) 198
Kzo9I GATC 2 cut(s) 312, 569
LmnI GCTCC 4 cut(s) 302, 769, 796, 879
Lsp1109I GCAGC 1 cut(s) 788
MaeI CTAG 2 cut(s) 369, 907
MaeII ACGT 3 cut(s) 351, 718, 810
MaeIII GTNAC 1 cut(s) 524
MalI GATC 2 cut(s) 314, 571
MboI GATC 2 cut(s) 312, 569
MboII GAAGA 7 cut(s) 90, 93, 96, 219, 590, 779, 848
MflI RGATCY 1 cut(s) 569
MhlI GDGCHC 3 cut(s) 299, 705, 801
MluCI AATT 2 cut(s) 339, 420
MmeI TCCRAC 1 cut(s) 324
MroI TCCGGA 1 cut(s) 876
MseI TTAA 2 cut(s) 176, 330
MspA1I CMGCKG 2 cut(s) 197, 470
MspI CCGG 3 cut(s) 288, 542, 877
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 4 cut(s) 122, 398, 653, 662
NdeII GATC 2 cut(s) 312, 569
NlaIII CATG 2 cut(s) 139, 320
NlaIV GGNNCC 2 cut(s) 298, 391
NmeAIII GCCGAG 1 cut(s) 790
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 1 cut(s) 414
PciI ACATGT 1 cut(s) 135
PfeI GAWTC 2 cut(s) 46, 256
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 608
PinAI ACCGGT 1 cut(s) 541
PkrI GCNGC 1 cut(s) 778
Ppu21I YACGTR 1 cut(s) 811
PscI ACATGT 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 298, 391
PspPI GGNCC 2 cut(s) 637, 677
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 569
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 1 cut(s) 809
RsaNI GTAC 1 cut(s) 808
SacII CCGCGG 1 cut(s) 198
SaqAI TTAA 2 cut(s) 176, 330
SatI GCNGC 1 cut(s) 777
Sau3AI GATC 2 cut(s) 312, 569
Sau96I GGNCC 2 cut(s) 637, 677
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 3 cut(s) 299, 705, 801
Sfr303I CCGCGG 1 cut(s) 198
SgrBI CCGCGG 1 cut(s) 198
SinI GGWCC 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 2 cut(s) 339, 420
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 5 cut(s) 114, 195, 197, 291, 384
SspMI CTAG 2 cut(s) 369, 907
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 516
TaaI ACNGT 3 cut(s) 183, 335, 629
TaiI ACGT 3 cut(s) 354, 721, 813
TaqI TCGA 3 cut(s) 259, 311, 696
TasI AATT 2 cut(s) 339, 420
TfiI GAWTC 2 cut(s) 46, 256
Tru1I TTAA 2 cut(s) 176, 330
Tru9I TTAA 2 cut(s) 176, 330
TscAI CASTG 2 cut(s) 188, 766
TseI GCWGC 1 cut(s) 776
TspDTI ATGAA 2 cut(s) 333, 647
TspGWI ACGGA 4 cut(s) 277, 296, 471, 856
TspRI CASTG 2 cut(s) 188, 766
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 637
XceI RCATGY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 2 cut(s) 369, 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.