pycom10g27980

Phosphoribosylglycinamide formyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013535
Physical Location & Seq
Forward (+)
28773733 .. 28776019
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27980.2

Sequence Viewer

Length: 924 bp
ATGGAAGCTCAGCGTCTGCTTTCTGGATTCTGCTCACCCTCGCCGATTCCAAACGCCAGAACCCAATACTTCGTCAGATTTCCCTCCCCTTCTCCTTCTCCTTCTTCTTCTTCTTCTTCTTCCTCCGCCGCTTCTTTTGCTCAATCCCACAGGTGGGTCTCCTTCAAAGCTCGCCCTTTGTCTACCAAGCTAATACAGTGCAGGAACACCGCGGACACAGAGAGAGCTGAGGTGGTGGTTTCTTCGGATAAACAAGATTTGACAAGTGGGATTCGACGGAAAAAGCTTGCCGTTTTTGTTTCCGGCGGGGGCTCCAACTTCCGTTCGATCCATGAAGCGTGTCTTAACGGTTTAATTCTCGGAGACGTTGTGGTTTTGGTCGCAAGTAAACAAGGTTGTGGAGGCGCCGACTATGCAAGAGAAAAAGGCCTCCCAATTATGGTGTTCCCAAAAACTAAACTTGAGCCTGACGGAATATCTCCAGCTGACCTTGTTGCTACCTTTAGGGGATTAGAGGTTGACTTTGTTCTCCTGGCTGGTTACTTGAAACTTATACCGGTTGAGTTGATACAAGCTTATCCCAGATCCATACTGAACATCCATCCCTCACTTCTTCCAGCATTTGGAGGCAAAGGTCATTACGGTATGAAGGTCCACAAGGCAGTCATTGCTTCTGGGGCAAGATACACAGGCCCTACAATACATTTTGTCGATGAGCACTATGATACAGGACGTATCCTTGCCCAAAGAGTTGTCCCTGTGCTTGCTAAGGACACTGCCGAGGAGCTGGCAGCAAGGGTTCTTCGGGAGGAGCACTCCTTGTACGTGGAAGTGATAACAGCAGTATGTGAAGAACGGATGGCTTGGAGGGACGATGGTGTTCCTATTATCCGGAGCAAAGAAAATCCCAACGAATACAGCTAG

Protein Analysis

308

Amino Acids

33.45

Weight (kDa)

8.9

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31220
fragaria_vesca FvH4_5g23990
malus_domestica MD03G1153200.v1.1 MD10G1330400.v1.1
prunus_persica Prupe.4G012000_v2.0.a1
pyrus_communis pycom03g10680 pycom10g27980
rosa_chinensis RchiOBHm_Chr7g0213451
rosa_laevigata RLG00000002803
rosa_multiflora Rmu_sc0013012.1_g000010 Rmu_ssc0000262.1_g000002
rosa_roxburghii Rroxscaffold_3G00245870
rosa_rugosa Rorug07G0142400
rosa_samantha Rh7AG274400 Rh7BG269900 Rh7CG293600 Rh7DG281900
rosa_wichuraiana Rw7G023800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 623
AccI GTMKAC 1 cut(s) 182
AccII CGCG 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 891
AciI CCGC 5 cut(s) 126, 129, 210, 212, 306
AclWI GGATC 2 cut(s) 322, 579
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 1 cut(s) 824
AfiI CCNNNNNNNGG 4 cut(s) 153, 154, 439, 623
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 2 cut(s) 166, 547
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 9 cut(s) 8, 170, 190, 227, 286, 485, 575, 787, 921
AluI AGCT 9 cut(s) 8, 170, 190, 227, 286, 485, 575, 787, 921
Alw21I GWGCWC 2 cut(s) 720, 816
Alw26I GTCTC 2 cut(s) 163, 357
AlwI GGATC 2 cut(s) 322, 579
AlwNI CAGNNNCTG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 891
AoxI GGCC 2 cut(s) 427, 691
ApeKI GCWGC 1 cut(s) 791
AsiGI ACCGGT 1 cut(s) 556
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 2 cut(s) 652, 692
AsuHPI GGTGA 1 cut(s) 27
AvaII GGWCC 1 cut(s) 652
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 2 cut(s) 720, 816
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 803
BccI CCATC 3 cut(s) 609, 853, 869
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 533
BciVI GTATCC 1 cut(s) 746
BcoDI GTCTC 2 cut(s) 163, 357
BfaI CTAG 1 cut(s) 922
BfoI RGCGCY 1 cut(s) 408
BfuI GTATCC 1 cut(s) 746
BisI GCNGC 2 cut(s) 129, 792
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 2 cut(s) 130, 793
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 652
BmgT120I GGNCC 2 cut(s) 652, 692
BmiI GGNNCC 2 cut(s) 313, 406
BmrFI CCNGG 1 cut(s) 533
BplI GAGNNNNNCTC 2 cut(s) 800, 832
BpmI CTGGAG 1 cut(s) 465
Bpu10I CCTNAGC 2 cut(s) 228, 768
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 482
BsaAI YACGTR 1 cut(s) 826
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 210, 780
BsaWI WCCGGW 2 cut(s) 556, 891
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 154, 439, 623
Bse118I RCCGGY 1 cut(s) 556
Bse3DI GCAATG 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 891
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 210, 780
BseGI GGATG 3 cut(s) 597, 601, 864
BseLI CCNNNNNNNGG 4 cut(s) 153, 154, 439, 623
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 23, 219
BseRI GAGGAG 2 cut(s) 797, 824
BseXI GCAGC 1 cut(s) 803
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 2 cut(s) 429, 693
BshNI GGYRCC 1 cut(s) 404
BshTI ACCGGT 1 cut(s) 556
BsiHKAI GWGCWC 2 cut(s) 720, 816
BsiSI CCGG 3 cut(s) 303, 557, 892
BslFI GGGAC 2 cut(s) 740, 884
BslI CCNNNNNNNGG 4 cut(s) 153, 154, 439, 623
BsmAI GTCTC 2 cut(s) 163, 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 740, 884
BsnI GGCC 2 cut(s) 429, 693
Bso31I GGTCTC 1 cut(s) 163
Bsp1286I GDGCHC 3 cut(s) 314, 720, 816
Bsp13I TCCGGA 1 cut(s) 891
Bsp143I GATC 2 cut(s) 327, 584
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 5 cut(s) 126, 129, 210, 212, 306
BspANI GGCC 2 cut(s) 429, 693
BspCNI CTCAG 2 cut(s) 22, 220
BspEI TCCGGA 1 cut(s) 891
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 313, 406
BspPI GGATC 2 cut(s) 322, 579
BspT107I GGYRCC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 210, 780
BssMI GATC 2 cut(s) 327, 584
BssNI GRCGYC 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 198, 350, 644
BstACI GRCGYC 1 cut(s) 405
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 4 cut(s) 172, 288, 765, 789
BstDEI CTNAG 3 cut(s) 9, 228, 768
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 597, 601, 864
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 2 cut(s) 330, 587
BstMAI GTCTC 2 cut(s) 163, 357
BstMBI GATC 2 cut(s) 327, 584
BstMWI GCNNNNNNNGC 4 cut(s) 137, 413, 668, 677
BstNI CCWGG 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 531
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 803
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BsuI GTATCC 1 cut(s) 746
BsuRI GGCC 2 cut(s) 429, 693
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 3 cut(s) 597, 601, 864
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 2 cut(s) 203, 774
Cac8I GCNNGC 4 cut(s) 172, 288, 765, 789
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 407
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 652, 692
Cfr42I CCGCGG 1 cut(s) 213
CseI GACGC 1 cut(s) 2
Csp6I GTAC 1 cut(s) 823
CspAI ACCGGT 1 cut(s) 556
CviAII CATG 1 cut(s) 332
CviQI GTAC 1 cut(s) 823
DdeI CTNAG 3 cut(s) 9, 228, 768
DinI GGCGCC 1 cut(s) 406
DpnI GATC 2 cut(s) 329, 586
DpnII GATC 2 cut(s) 327, 584
EciI GGCGGA 1 cut(s) 115
Eco147I AGGCCT 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 314
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 1 cut(s) 335
FaiI YATR 8 cut(s) 333, 414, 440, 554, 590, 647, 723, 847
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 2 cut(s) 740, 884
FatI CATG 1 cut(s) 331
FauI CCCGC 1 cut(s) 299
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 2 cut(s) 129, 792
FokI GGATG 3 cut(s) 584, 588, 871
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 129, 792
FspBI CTAG 1 cut(s) 922
GlaI GCGC 1 cut(s) 406
GluI GCNGC 2 cut(s) 129, 792
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 2 cut(s) 429, 693
HapII CCGG 3 cut(s) 303, 557, 892
HgaI GACGC 1 cut(s) 2
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 1 cut(s) 335
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 2 cut(s) 284, 573
HinfI GANTC 3 cut(s) 27, 46, 271
HpaII CCGG 3 cut(s) 303, 557, 892
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 4 cut(s) 183, 389, 520, 655
Hpy188I TCNGA 3 cut(s) 77, 247, 362
Hpy188III TCNNGA 3 cut(s) 24, 806, 892
Hpy8I GTNNAC 4 cut(s) 183, 389, 520, 655
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 5 cut(s) 99, 105, 111, 172, 643
HpyCH4III ACNGT 3 cut(s) 198, 350, 644
HpyCH4IV ACGT 3 cut(s) 366, 733, 825
HpyCH4V TGCA 2 cut(s) 201, 416
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 413, 668, 677
HpyF3I CTNAG 3 cut(s) 9, 228, 768
HpySE526I ACGT 3 cut(s) 366, 733, 825
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 1 cut(s) 335
HspAI GCGC 1 cut(s) 405
KasI GGCGCC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 891
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 2 cut(s) 327, 584
LmnI GCTCC 4 cut(s) 317, 784, 811, 894
Lsp1109I GCAGC 1 cut(s) 803
MaeI CTAG 1 cut(s) 922
MaeII ACGT 3 cut(s) 366, 733, 825
MaeIII GTNAC 1 cut(s) 539
MalI GATC 2 cut(s) 329, 586
MboI GATC 2 cut(s) 327, 584
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 3 cut(s) 314, 720, 816
MluCI AATT 2 cut(s) 354, 435
Mly113I GGCGCC 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 339
MroI TCCGGA 1 cut(s) 891
MseI TTAA 2 cut(s) 345, 353
MspA1I CMGCKG 2 cut(s) 212, 485
MspI CCGG 3 cut(s) 303, 557, 892
MspR9I CCNGG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 4 cut(s) 137, 413, 668, 677
NarI GGCGCC 1 cut(s) 405
NdeII GATC 2 cut(s) 327, 584
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 2 cut(s) 313, 406
NmeAIII GCCGAG 1 cut(s) 805
PceI AGGCCT 1 cut(s) 429
PfeI GAWTC 3 cut(s) 27, 46, 271
PflMI CCANNNNNTGG 1 cut(s) 623
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 2 cut(s) 130, 793
PluTI GGCGCC 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 826
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 2 cut(s) 313, 406
PspPI GGNCC 2 cut(s) 652, 692
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 824
RsaNI GTAC 1 cut(s) 823
SacII CCGCGG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 345, 353
SatI GCNGC 2 cut(s) 129, 792
Sau3AI GATC 2 cut(s) 327, 584
Sau96I GGNCC 2 cut(s) 652, 692
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 3 cut(s) 314, 720, 816
SfoI GGCGCC 1 cut(s) 406
Sfr303I CCGCGG 1 cut(s) 213
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 1 cut(s) 652
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 2 cut(s) 354, 435
SseBI AGGCCT 1 cut(s) 429
SsiI CCGC 5 cut(s) 126, 129, 210, 212, 306
SspDI GGCGCC 1 cut(s) 404
SspMI CTAG 1 cut(s) 922
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 198, 350, 644
TaiI ACGT 3 cut(s) 369, 736, 828
TaqI TCGA 3 cut(s) 274, 326, 711
TasI AATT 2 cut(s) 354, 435
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 3 cut(s) 27, 46, 271
Tru1I TTAA 2 cut(s) 345, 353
Tru9I TTAA 2 cut(s) 345, 353
TscAI CASTG 2 cut(s) 203, 781
TseI GCWGC 1 cut(s) 791
TspDTI ATGAA 2 cut(s) 348, 662
TspGWI ACGGA 4 cut(s) 292, 311, 486, 871
TspRI CASTG 2 cut(s) 203, 781
Van91I CCANNNNNTGG 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 652
XmiI GTMKAC 1 cut(s) 182
XspI CTAG 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.