MD03G1159500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011391
Physical Location & Seq
Forward (+)
18982713 .. 19005788
23076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1159500.v1.1.491

Sequence Viewer

Length: 855 bp
ATGATGAGAAGGCAGGGGCAGTATGGTGATTCGGGTGCGAATGCATATGCCACGGCTGCTCCGATGCACCATATGTCTGGTCAAAGGATGGAGCACAATTCTAGCCATTTCGAAGAAAGGCTGGAAGCTTTCACTCCACCTCCAGAGAGGGAGAATCCGTATGCAACTTCAAAATCAGAGGACCAGTGGAGATGGGAAAGAGATGGGTCCAAAGGGTCAAATCCAGTGACATCTCATATGTACAATGAAGGTCAAGGAGGTGATACATCAAGATCCTATTTCCAGGGTCAAAGGCCTGAATCAAAACATTCTTTGGAGAAACAGAGCAGCAACGATTCCCAATCTCAACCACTTAATGAAGCAATGGACCTTGGATATGAGGACAAGCCTTCATTGCAGACTTTTGAAGATTTTGAGAAGAAGTTCTTTGATGACATTAAAAAACTAGTGCATGAACAAAGTGATGCTGAGGATGCAGAAAATGCTAGGCATAGAGAGAAAATTGGAGCGGTCAATGCTCAATACCAAGAACAGCTGGCAGCACTTCGGGCACGGCATGCTAAACGTAGAGATGAATTACTTGAGAAGGAATCAAATGCCCGACAGCATCAGTACCAGCAATTGGTGACAGATCGTTACCCGAACAGCAGCATGGGCGCCTCTGATCATCATGGGTACAGTGGAGTTGCAGCCTCGGCTCCTGTTGGTGAAGCACACCGAGGGTATAATACTGATCAACATGATTCCTACAGTGAGAGGGCTCGATTTCTTGGGGGTTCTCGGGATCATCGGTTTGAGCCTAGAGGTCCATATCCTGGAGGTCGCGTCTATGATACTGGTTCACGGTATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

32.44

Weight (kDa)

6.01

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22040 AT5G22040 AT5G22040
fragaria_vesca FvH4_3g26150 FvH4_3g26150
malus_domestica MD03G1159500.v1.1 MD11G1174800.v1.1
prunus_persica Prupe.6G147200_v2.0.a1
pyrus_communis pycom03g11210 pycom11g14950
rosa_chinensis RchiOBHm_Chr5g0049091
rosa_laevigata RLG00000034588
rosa_multiflora Rmu_co8392037.1_g000001
rosa_roxburghii Rroxscaffold_1G00032780
rosa_rugosa Rorug05G0243100
rosa_samantha Rh5AG322600 Rh5BG333200 Rh5CG358100 Rh5DG344600
rosa_wichuraiana Rw5G030440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 656
AccB7I CCANNNNNTGG 2 cut(s) 622, 815
AccBSI CCGCTC 1 cut(s) 509
AccII CGCG 1 cut(s) 825
AciI CCGC 1 cut(s) 509
AclWI GGATC 2 cut(s) 267, 792
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 3 cut(s) 242, 614, 677
AfiI CCNNNNNNNGG 2 cut(s) 622, 815
AgsI TTSAA 2 cut(s) 171, 407
AhlI ACTAGT 1 cut(s) 445
AjnI CCWGG 2 cut(s) 282, 814
AluBI AGCT 2 cut(s) 128, 535
AluI AGCT 2 cut(s) 128, 535
Alw21I GWGCWC 1 cut(s) 96
AlwI GGATC 2 cut(s) 267, 792
Ama87I CYCGRG 1 cut(s) 780
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 5 cut(s) 56, 327, 539, 648, 689
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 4 cut(s) 181, 207, 367, 806
AsuHPI GGTGA 4 cut(s) 38, 272, 637, 719
AsuII TTCGAA 1 cut(s) 111
AvaI CYCGRG 1 cut(s) 780
AvaII GGWCC 4 cut(s) 181, 207, 367, 806
BaeGI GKGCMC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 763
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 468
BbvI GCAGC 5 cut(s) 43, 339, 551, 660, 701
BccI CCATC 3 cut(s) 82, 186, 197
BceAI ACGGC 2 cut(s) 69, 569
BciT130I CCWGG 2 cut(s) 284, 816
BclI TGATCA 2 cut(s) 664, 733
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 4 cut(s) 102, 446, 486, 801
BfmI CTRYAG 1 cut(s) 748
BfoI RGCGCY 1 cut(s) 660
BisI GCNGC 5 cut(s) 57, 328, 540, 649, 690
BlsI GCNGC 5 cut(s) 58, 329, 541, 650, 691
Bme1390I CCNGG 2 cut(s) 284, 816
Bme18I GGWCC 4 cut(s) 181, 207, 367, 806
BmeT110I CYCGRG 1 cut(s) 780
BmgT120I GGNCC 4 cut(s) 181, 207, 367, 806
BmiI GGNNCC 3 cut(s) 208, 658, 699
BmrFI CCNGG 2 cut(s) 284, 816
BmsI GCATC 4 cut(s) 54, 454, 463, 616
BpmI CTGGAG 2 cut(s) 126, 837
Bpu10I CCTNAGC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 657
BsaJI CCNNGG 5 cut(s) 51, 283, 370, 693, 718
BsaXI ACNNNNNCTCC 8 cut(s) 43, 73, 124, 143, 154, 173, 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 815
Bse1I ACTGG 3 cut(s) 184, 224, 841
Bse3DI GCAATG 2 cut(s) 369, 392
BseBI CCWGG 2 cut(s) 284, 816
BseDI CCNNGG 5 cut(s) 51, 283, 370, 693, 718
BseGI GGATG 2 cut(s) 93, 478
BseLI CCNNNNNNNGG 2 cut(s) 622, 815
BseMI GCAATG 2 cut(s) 369, 392
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 3 cut(s) 184, 224, 841
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 5 cut(s) 43, 339, 551, 660, 701
Bsh1236I CGCG 1 cut(s) 825
BshFI GGCC 1 cut(s) 295
BshNI GGYRCC 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 780
BslI CCNNNNNNNGG 2 cut(s) 622, 815
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 1 cut(s) 295
BsoBI CYCGRG 1 cut(s) 780
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 96, 553, 763
Bsp1407I TGTACA 1 cut(s) 240
Bsp143I GATC 5 cut(s) 272, 631, 664, 733, 784
BspACI CCGC 1 cut(s) 509
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 208, 658, 699
BspPI GGATC 2 cut(s) 267, 792
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 369, 392
BsrGI TGTACA 1 cut(s) 240
BsrI ACTGG 3 cut(s) 184, 224, 841
BssECI CCNNGG 5 cut(s) 51, 283, 370, 693, 718
BssMI GATC 5 cut(s) 272, 631, 664, 733, 784
BssNI GRCGYC 1 cut(s) 657
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 284, 816
Bst4CI ACNGT 3 cut(s) 680, 752, 846
BstACI GRCGYC 1 cut(s) 657
BstAPI GCANNNNNTGC 2 cut(s) 482, 557
BstAUI TGTACA 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 537, 558
BstDEI CTNAG 1 cut(s) 468
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 93, 478
BstFNI CGCG 1 cut(s) 825
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 5 cut(s) 275, 634, 667, 736, 787
BstMBI GATC 5 cut(s) 272, 631, 664, 733, 784
BstMWI GCNNNNNNNGC 9 cut(s) 56, 394, 473, 482, 515, 548, 557, 654, 695
BstNI CCWGG 2 cut(s) 284, 816
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 282, 814
BstSFI CTRYAG 1 cut(s) 748
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 5 cut(s) 43, 339, 551, 660, 701
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 77
BstYI RGATCY 1 cut(s) 272
BsuRI GGCC 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 93, 478
BtsIMutI CAGTG 4 cut(s) 191, 231, 685, 757
Cac8I GCNNGC 2 cut(s) 537, 558
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 4 cut(s) 181, 207, 367, 806
CseI GACGC 1 cut(s) 814
Csp6I GTAC 3 cut(s) 241, 613, 676
CviAII CATG 5 cut(s) 452, 557, 652, 671, 740
CviQI GTAC 3 cut(s) 241, 613, 676
DdeI CTNAG 1 cut(s) 468
DinI GGCGCC 1 cut(s) 658
DpnI GATC 5 cut(s) 274, 633, 666, 735, 786
DpnII GATC 5 cut(s) 272, 631, 664, 733, 784
Eco130I CCWWGG 1 cut(s) 370
Eco147I AGGCCT 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 763
Eco47I GGWCC 4 cut(s) 181, 207, 367, 806
Eco88I CYCGRG 1 cut(s) 780
EcoRII CCWGG 2 cut(s) 282, 814
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 763
EgeI GGCGCC 1 cut(s) 658
EheI GGCGCC 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 455, 560, 655, 674, 743
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 5 cut(s) 451, 556, 651, 670, 739
FauNDI CATATG 3 cut(s) 46, 72, 237
FbaI TGATCA 2 cut(s) 664, 733
Fnu4HI GCNGC 5 cut(s) 57, 328, 540, 649, 690
FokI GGATG 2 cut(s) 100, 485
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 5 cut(s) 57, 328, 540, 649, 690
FspBI CTAG 4 cut(s) 102, 446, 486, 801
GlaI GCGC 1 cut(s) 658
GluI GCNGC 5 cut(s) 57, 328, 540, 649, 690
GsuI CTGGAG 2 cut(s) 126, 837
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 1 cut(s) 295
HgaI GACGC 1 cut(s) 814
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 5 cut(s) 455, 560, 655, 674, 743
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 6 cut(s) 29, 154, 299, 335, 590, 743
HphI GGTGA 4 cut(s) 38, 272, 637, 719
Hpy166II GTNNAC 1 cut(s) 842
Hpy188I TCNGA 3 cut(s) 63, 178, 664
Hpy188III TCNNGA 3 cut(s) 143, 270, 782
Hpy8I GTNNAC 1 cut(s) 842
HpyAV CCTTC 4 cut(s) 3, 242, 399, 580
HpyCH4III ACNGT 3 cut(s) 680, 752, 846
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 7 cut(s) 44, 67, 164, 397, 451, 476, 689
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 394, 473, 482, 515, 548, 557, 654, 695
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 1 cut(s) 565
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 5 cut(s) 455, 560, 655, 674, 743
HspAI GCGC 1 cut(s) 657
KasI GGCGCC 1 cut(s) 656
Ksp22I TGATCA 2 cut(s) 664, 733
Kzo9I GATC 5 cut(s) 272, 631, 664, 733, 784
LmnI GCTCC 4 cut(s) 64, 91, 506, 703
Lsp1109I GCAGC 5 cut(s) 43, 339, 551, 660, 701
LweI GCATC 4 cut(s) 54, 454, 463, 616
MaeI CTAG 4 cut(s) 102, 446, 486, 801
MaeII ACGT 1 cut(s) 565
MaeIII GTNAC 3 cut(s) 226, 625, 635
MalI GATC 5 cut(s) 274, 633, 666, 735, 786
MbiI CCGCTC 1 cut(s) 509
MboI GATC 5 cut(s) 272, 631, 664, 733, 784
MboII GAAGA 3 cut(s) 125, 419, 430
MfeI CAATTG 1 cut(s) 620
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 3 cut(s) 96, 553, 763
MluCI AATT 4 cut(s) 97, 501, 575, 620
Mly113I GGCGCC 1 cut(s) 657
Mph1103I ATGCAT 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 2 cut(s) 354, 438
MspA1I CMGCKG 1 cut(s) 535
MspR9I CCNGG 2 cut(s) 284, 816
MunI CAATTG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 2 cut(s) 284, 816
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 9 cut(s) 56, 394, 473, 482, 515, 548, 557, 654, 695
NarI GGCGCC 1 cut(s) 657
NdeI CATATG 3 cut(s) 46, 72, 237
NdeII GATC 5 cut(s) 272, 631, 664, 733, 784
NlaIII CATG 5 cut(s) 455, 560, 655, 674, 743
NlaIV GGNNCC 3 cut(s) 208, 658, 699
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 2 cut(s) 226, 625
NsiI ATGCAT 1 cut(s) 46
NspI RCATGY 1 cut(s) 560
NspV TTCGAA 1 cut(s) 111
PaeI GCATGC 1 cut(s) 560
PceI AGGCCT 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 6 cut(s) 29, 154, 299, 335, 590, 743
PflMI CCANNNNNTGG 2 cut(s) 622, 815
PfoI TCCNGGA 1 cut(s) 814
PkrI GCNGC 5 cut(s) 58, 329, 541, 650, 691
PluTI GGCGCC 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 282, 814
PspGI CCWGG 2 cut(s) 282, 814
PspN4I GGNNCC 3 cut(s) 208, 658, 699
PspPI GGNCC 4 cut(s) 181, 207, 367, 806
PsuI RGATCY 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 3 cut(s) 242, 614, 677
RsaNI GTAC 3 cut(s) 241, 613, 676
SaqAI TTAA 2 cut(s) 354, 438
SatI GCNGC 5 cut(s) 57, 328, 540, 649, 690
Sau3AI GATC 5 cut(s) 272, 631, 664, 733, 784
Sau96I GGNCC 4 cut(s) 181, 207, 367, 806
ScrFI CCNGG 2 cut(s) 284, 816
SduI GDGCHC 3 cut(s) 96, 553, 763
SetI ASST 9 cut(s) 130, 142, 253, 262, 372, 537, 568, 808, 823
SfaNI GCATC 4 cut(s) 54, 454, 463, 616
SfcI CTRYAG 1 cut(s) 748
SfoI GGCGCC 1 cut(s) 658
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 4 cut(s) 181, 207, 367, 806
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SpeI ACTAGT 1 cut(s) 445
SphI GCATGC 1 cut(s) 560
Sse9I AATT 4 cut(s) 97, 501, 575, 620
SseBI AGGCCT 1 cut(s) 295
SsiI CCGC 1 cut(s) 509
SspDI GGCGCC 1 cut(s) 656
SspMI CTAG 4 cut(s) 102, 446, 486, 801
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 2 cut(s) 282, 814
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 3 cut(s) 680, 752, 846
TaiI ACGT 1 cut(s) 568
TaqI TCGA 2 cut(s) 111, 763
TasI AATT 4 cut(s) 97, 501, 575, 620
TatI WGTACW 1 cut(s) 240
TfiI GAWTC 6 cut(s) 29, 154, 299, 335, 590, 743
Tru1I TTAA 2 cut(s) 354, 438
Tru9I TTAA 2 cut(s) 354, 438
TscAI CASTG 4 cut(s) 191, 231, 685, 757
TseFI GTSAC 2 cut(s) 226, 625
TseI GCWGC 5 cut(s) 56, 327, 539, 648, 689
Tsp45I GTSAC 2 cut(s) 226, 625
TspDTI ATGAA 5 cut(s) 261, 372, 381, 468, 588
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 4 cut(s) 191, 231, 685, 757
Van91I CCANNNNNTGG 2 cut(s) 622, 815
VpaK11BI GGWCC 4 cut(s) 181, 207, 367, 806
XceI RCATGY 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 4 cut(s) 102, 446, 486, 801
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.