Rroxscaffold_1G00032780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011391
Physical Location & Seq
Reverse (-)
47129609 .. 47133365
3757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00032780.1

Sequence Viewer

Length: 924 bp
ATGATGAGACGCCAGCAGGCGCAGTACGCCGGCGATTCAGGCGGCGCCAATTCGTATGGTGGCGGAGGCGGTGGTGCTGCGGCAGCTTCAATGCACCACCACCACCACCATCATCAGCAGCAGCAGCCGAGTAGTGTTGGTGCTAGTGATTTTGAAGGACGACTCGAAGCGTTCACACCCGAGAGGGATAGCAATCCTAATCCTAATCCATACGCTACTTCTTCCAAACCAGAGGCCCAGTGGAGATGGGAAAGAGATGCATCCAATTCCAAGGCCTCCAATCCATTGACACCTCACATGTTTAGTGAAGGCAAGTCTTGTCAAGTGGGTGATACATCTAGATCCTATTTCCAGGGTCAAAGGTCCGATCCAAAACATGCTTTAGAGACACAGAGCAACAGCGATCCCCGATCTCAACCTCATATTGAAGATATGGACCTTGGGTATGAGGATAAGCCTTCGTCACAGTCCTTTGAAGTTCTGGAGCAGAAATTCCTTGATGATTTTAGGAAACTAACCAAGGAACAGAATGATGCTGAGGATGCAGAAAATGCTAGACACAGAGAGAAAATTGGTACAATCAATGCTCAGTATGAAGAACAATTAGCATCACTACGGTGCCAGCATGCTGGCCGTAGAGATGATTTACTTCGGAGGGAATCAAATGCCCGGCAGCATCAATACCAGCAATCCATGATGGATCGTTACCCTAAAAGCAGCATGGGCTCCTCCGATCTTCATGGTTACAGTGGAATTGCGGCCTCAGCTACTGCTGGCGATACACGCAGAGGCTACGAAACTGACCAATATGATTCTTACAGAGAGCGGGCTCGATTTCTTGGGGGCTCCCGTGATCATGGGTTTGAGCCTAGGGGACCATATCCGGGAGGGCGTGTTTATGATACTGGCTCACGTTATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.52

Weight (kDa)

6.2

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22040 AT5G22040 AT5G22040
fragaria_vesca FvH4_3g26150 FvH4_3g26150
malus_domestica MD03G1159500.v1.1 MD11G1174800.v1.1
prunus_persica Prupe.6G147200_v2.0.a1
pyrus_communis pycom03g11210 pycom11g14950
rosa_chinensis RchiOBHm_Chr5g0049091
rosa_laevigata RLG00000034588
rosa_multiflora Rmu_co8392037.1_g000001
rosa_roxburghii Rroxscaffold_1G00032780
rosa_rugosa Rorug05G0243100
rosa_samantha Rh5AG322600 Rh5BG333200 Rh5CG358100 Rh5DG344600
rosa_wichuraiana Rw5G030440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 44, 618
AccBSI CCGCTC 1 cut(s) 826
AciI CCGC 6 cut(s) 42, 63, 69, 80, 758, 826
AclWI GGATC 4 cut(s) 336, 362, 398, 708
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 1 cut(s) 491
AcyI GRCGYC 2 cut(s) 10, 45
AfaI GTAC 2 cut(s) 26, 577
AfiI CCNNNNNNNGG 1 cut(s) 884
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 4 cut(s) 90, 155, 428, 476
AjnI CCWGG 1 cut(s) 351
AleI CACNNNNGTG 1 cut(s) 616
AluBI AGCT 2 cut(s) 86, 767
AluI AGCT 2 cut(s) 86, 767
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 4 cut(s) 336, 362, 398, 708
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 234, 273, 631, 759
ApeKI GCWGC 7 cut(s) 77, 83, 118, 121, 124, 673, 717
ApoI RAATTY 1 cut(s) 491
AspA2I CCTAGG 1 cut(s) 869
AspLEI GCGC 2 cut(s) 22, 47
AspS9I GGNCC 4 cut(s) 235, 363, 436, 875
AsuC2I CCSGG 2 cut(s) 670, 885
AsuHPI GGTGA 1 cut(s) 341
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 3 cut(s) 363, 436, 875
AvrII CCTAGG 1 cut(s) 869
BanI GGYRCC 2 cut(s) 44, 618
BanII GRGCYC 3 cut(s) 728, 832, 848
BbvCI CCTCAGC 2 cut(s) 537, 763
BbvI GCAGC 7 cut(s) 64, 95, 130, 133, 136, 685, 729
BccI CCATC 3 cut(s) 117, 240, 691
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 853
BcnI CCSGG 2 cut(s) 670, 885
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 4 cut(s) 144, 339, 555, 870
BfoI RGCGCY 1 cut(s) 48
BlnI CCTAGG 1 cut(s) 869
Bme1390I CCNGG 3 cut(s) 353, 670, 885
Bme18I GGWCC 3 cut(s) 363, 436, 875
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 4 cut(s) 235, 363, 436, 875
BmiI GGNNCC 5 cut(s) 46, 620, 727, 847, 876
BmrFI CCNGG 3 cut(s) 353, 670, 885
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 6 cut(s) 247, 269, 523, 532, 617, 685
BmuI ACTGGG 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 2 cut(s) 537, 763
BpuMI CCSGG 2 cut(s) 670, 885
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 10, 45
BsaJI CCNNGG 5 cut(s) 270, 352, 439, 519, 869
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 1 cut(s) 884
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 238, 910
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 5 cut(s) 270, 352, 439, 519, 869
BseGI GGATG 2 cut(s) 260, 547
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 884
BseMII CTCAG 3 cut(s) 528, 602, 777
BseNI ACTGG 2 cut(s) 238, 910
BseRI GAGGAG 1 cut(s) 718
BseXI GCAGC 7 cut(s) 64, 95, 130, 133, 136, 685, 729
BshFI GGCC 4 cut(s) 236, 275, 633, 761
BshNI GGYRCC 2 cut(s) 44, 618
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 3 cut(s) 30, 670, 884
BslFI GGGAC 1 cut(s) 888
BslI CCNNNNNNNGG 1 cut(s) 884
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 888
BsnI GGCC 4 cut(s) 236, 275, 633, 761
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 728, 832, 848
Bsp143I GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
BspACI CCGC 6 cut(s) 42, 63, 69, 80, 758, 826
BspANI GGCC 4 cut(s) 236, 275, 633, 761
BspCNI CTCAG 3 cut(s) 529, 601, 776
BspLI GGNNCC 5 cut(s) 46, 620, 727, 847, 876
BspPI GGATC 4 cut(s) 336, 362, 398, 708
BspT107I GGYRCC 2 cut(s) 44, 618
BsrBI CCGCTC 1 cut(s) 826
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 2 cut(s) 238, 910
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 5 cut(s) 270, 352, 439, 519, 869
BssMI GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
BssNI GRCGYC 2 cut(s) 10, 45
BssT1I CCWWGG 4 cut(s) 270, 439, 519, 869
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 3 cut(s) 468, 618, 749
BstACI GRCGYC 2 cut(s) 10, 45
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 8 cut(s) 14, 18, 31, 623, 627, 631, 775, 828
BstDEI CTNAG 3 cut(s) 537, 588, 763
BstF5I GGATG 2 cut(s) 260, 547
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 22, 47
BstKTI GATC 7 cut(s) 344, 370, 406, 413, 703, 736, 856
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
BstMWI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 542, 551, 723, 764, 783
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 3 cut(s) 301, 380, 629
BstSCI CCNGG 3 cut(s) 351, 668, 883
BstV1I GCAGC 7 cut(s) 64, 95, 130, 133, 136, 685, 729
BstX2I RGATCY 1 cut(s) 341
BstXI CCANNNNNNTGG 1 cut(s) 629
BstYI RGATCY 1 cut(s) 341
BsuRI GGCC 4 cut(s) 236, 275, 633, 761
BtsCI GGATG 2 cut(s) 260, 547
BtsIMutI CAGTG 2 cut(s) 245, 754
Cac8I GCNNGC 8 cut(s) 14, 18, 31, 623, 627, 631, 775, 828
CaiI CAGNNNCTG 1 cut(s) 770
CfoI GCGC 2 cut(s) 22, 47
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 235, 363, 436, 875
CseI GACGC 1 cut(s) 18
Csp6I GTAC 2 cut(s) 25, 576
CviAII CATG 7 cut(s) 298, 377, 626, 694, 721, 740, 857
CviQI GTAC 2 cut(s) 25, 576
DdeI CTNAG 3 cut(s) 537, 588, 763
DinI GGCGCC 1 cut(s) 46
DpnI GATC 7 cut(s) 343, 369, 405, 412, 702, 735, 855
DpnII GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
EaeI YGGCCR 1 cut(s) 631
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 4 cut(s) 270, 439, 519, 869
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 3 cut(s) 728, 832, 848
Eco47I GGWCC 3 cut(s) 363, 436, 875
Eco88I CYCGRG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 4 cut(s) 270, 439, 519, 869
EcoT22I ATGCAT 1 cut(s) 262
EcoT38I GRGCYC 3 cut(s) 728, 832, 848
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 4 cut(s) 270, 439, 519, 869
FaeI CATG 7 cut(s) 301, 380, 629, 697, 724, 743, 860
FaqI GGGAC 1 cut(s) 888
FatI CATG 7 cut(s) 297, 376, 625, 693, 720, 739, 856
FauI CCCGC 1 cut(s) 819
FbaI TGATCA 1 cut(s) 853
FokI GGATG 2 cut(s) 247, 554
FriOI GRGCYC 3 cut(s) 728, 832, 848
FspBI CTAG 4 cut(s) 144, 339, 555, 870
GlaI GCGC 2 cut(s) 21, 46
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 4 cut(s) 236, 275, 633, 761
HapII CCGG 3 cut(s) 30, 670, 884
HgaI GACGC 1 cut(s) 18
HhaI GCGC 2 cut(s) 22, 47
Hin1I GRCGYC 2 cut(s) 10, 45
Hin1II CATG 7 cut(s) 301, 380, 629, 697, 724, 743, 860
Hin6I GCGC 2 cut(s) 20, 45
HinP1I GCGC 2 cut(s) 20, 45
HinfI GANTC 4 cut(s) 35, 162, 659, 812
HpaII CCGG 3 cut(s) 30, 670, 884
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 3 cut(s) 367, 654, 733
Hpy188III TCNNGA 2 cut(s) 339, 482
Hpy8I GTNNAC 1 cut(s) 174
HpyAV CCTTC 3 cut(s) 149, 302, 468
HpyCH4III ACNGT 3 cut(s) 468, 618, 749
HpyCH4IV ACGT 1 cut(s) 913
HpyCH4V TGCA 3 cut(s) 94, 260, 545
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 542, 551, 723, 764, 783
HpyF3I CTNAG 3 cut(s) 537, 588, 763
HpySE526I ACGT 1 cut(s) 913
Hsp92I GRCGYC 2 cut(s) 10, 45
Hsp92II CATG 7 cut(s) 301, 380, 629, 697, 724, 743, 860
HspAI GCGC 2 cut(s) 20, 45
KasI GGCGCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
LmnI GCTCC 3 cut(s) 484, 731, 851
Lsp1109I GCAGC 7 cut(s) 64, 95, 130, 133, 136, 685, 729
LweI GCATC 6 cut(s) 247, 269, 523, 532, 617, 685
MaeI CTAG 4 cut(s) 144, 339, 555, 870
MaeII ACGT 1 cut(s) 913
MaeIII GTNAC 3 cut(s) 462, 704, 743
MalI GATC 7 cut(s) 343, 369, 405, 412, 702, 735, 855
MbiI CCGCTC 1 cut(s) 826
MboI GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
MboII GAAGA 4 cut(s) 213, 440, 608, 728
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 3 cut(s) 728, 832, 848
MluCI AATT 6 cut(s) 49, 265, 491, 570, 602, 753
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 1 cut(s) 156
Mph1103I ATGCAT 1 cut(s) 262
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 616
MspI CCGG 3 cut(s) 30, 670, 884
MspR9I CCNGG 3 cut(s) 353, 670, 885
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 542, 551, 723, 764, 783
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 2 cut(s) 670, 885
NdeII GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 7 cut(s) 301, 380, 629, 697, 724, 743, 860
NlaIV GGNNCC 5 cut(s) 46, 620, 727, 847, 876
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 462
NsiI ATGCAT 1 cut(s) 262
NspI RCATGY 3 cut(s) 301, 380, 629
OliI CACNNNNGTG 1 cut(s) 616
PaeI GCATGC 1 cut(s) 629
PceI AGGCCT 1 cut(s) 275
PciI ACATGT 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 3 cut(s) 35, 659, 812
PfoI TCCNGGA 1 cut(s) 883
PleI GAGTC 1 cut(s) 156
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 156
PscI ACATGT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 5 cut(s) 46, 620, 727, 847, 876
PspPI GGNCC 4 cut(s) 235, 363, 436, 875
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 341
RsaI GTAC 2 cut(s) 26, 577
RsaNI GTAC 2 cut(s) 25, 576
RseI CAYNNNNRTG 1 cut(s) 616
Sau3AI GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
Sau96I GGNCC 4 cut(s) 235, 363, 436, 875
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 353, 670, 885
SduI GDGCHC 3 cut(s) 728, 832, 848
SetI ASST 7 cut(s) 88, 295, 365, 421, 441, 769, 916
SfaNI GCATC 6 cut(s) 247, 269, 523, 532, 617, 685
SfoI GGCGCC 1 cut(s) 46
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 3 cut(s) 363, 436, 875
SmiMI CAYNNNNRTG 1 cut(s) 616
SphI GCATGC 1 cut(s) 629
Sse9I AATT 6 cut(s) 49, 265, 491, 570, 602, 753
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 6 cut(s) 42, 63, 69, 80, 758, 826
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 4 cut(s) 144, 339, 555, 870
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 351, 668, 883
StyI CCWWGG 4 cut(s) 270, 439, 519, 869
TaaI ACNGT 3 cut(s) 468, 618, 749
TaiI ACGT 1 cut(s) 916
TaqI TCGA 2 cut(s) 165, 832
TasI AATT 6 cut(s) 49, 265, 491, 570, 602, 753
TauI GCSGC 3 cut(s) 45, 83, 761
TfiI GAWTC 3 cut(s) 35, 659, 812
TscAI CASTG 2 cut(s) 245, 754
TseFI GTSAC 1 cut(s) 462
TseI GCWGC 7 cut(s) 77, 83, 118, 121, 124, 673, 717
Tsp45I GTSAC 1 cut(s) 462
TspDTI ATGAA 2 cut(s) 609, 728
TspRI CASTG 2 cut(s) 245, 754
VpaK11BI GGWCC 3 cut(s) 363, 436, 875
XapI RAATTY 1 cut(s) 491
XbaI TCTAGA 1 cut(s) 338
XceI RCATGY 3 cut(s) 301, 380, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 237
XmaJI CCTAGG 1 cut(s) 869
XspI CTAG 4 cut(s) 144, 339, 555, 870
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.