Rh5BG333200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011391
Physical Location & Seq
Forward (+)
46347243 .. 46351466
4224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG333200.1

Sequence Viewer

Length: 657 bp
ATGATGAGACGCCAGCAGGCGCAGTACGCCGGCGATTCAGGCGGCGCCAATTCGTATGGTGGCGGAGGCGGTGGCGCTGCGGCAGCTTCAATGCACCACCACCACCACCATCATCAGCAGCAGCAGCCGAGTAGTGTTGGTGCTAGTGATTTTGAAGGACGACTCGAAGCGTTCACACCCGAGAGGGATAGCAATCCTAATCCTAATCCATACGCTACTTCTTCCAAACCAGAGGCCCAGTGGAGATGGGAAAGAGATGCATCCAATTCCAAAGCCTCCAATCCATTGACACCTCACATGTTTAGTGAAGGTCAAGTGGGTGATACATCTAGATCCTATTTCCAGGGTCAAAGGTCCGATCCAAAACATGCTTTAGAGACACAGAGCAACAACGATCCCCGATCTCAACCTCATATTGAAGATATGGACCTTGGGTACGAGGATAAGCCTTCGTCACAGTCCTTTGAAGTTCTGGAGCAGAAATTCCTTGATGACATTAGGAAACTAACCAAGGAACAGAATGATGCTGAGGATGCAGAAAATGCTAGACACAGAGAGGTTTGTTCGTATTTGAAAGTGGATTATGTTCTCTTATATTTGGATTCCCTGTTTGTGTCTCCATTATCTTTCTACATTCTCCTGCCAATCTCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.4

Weight (kDa)

5.39

Isoelectric Point (pI)

57.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22040 AT5G22040 AT5G22040
fragaria_vesca FvH4_3g26150 FvH4_3g26150
malus_domestica MD03G1159500.v1.1 MD11G1174800.v1.1
prunus_persica Prupe.6G147200_v2.0.a1
pyrus_communis pycom03g11210 pycom11g14950
rosa_chinensis RchiOBHm_Chr5g0049091
rosa_laevigata RLG00000034588
rosa_multiflora Rmu_co8392037.1_g000001
rosa_roxburghii Rroxscaffold_1G00032780
rosa_rugosa Rorug05G0243100
rosa_samantha Rh5AG322600 Rh5BG333200 Rh5CG358100 Rh5DG344600
rosa_wichuraiana Rw5G030440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AciI CCGC 4 cut(s) 42, 63, 69, 80
AclWI GGATC 3 cut(s) 327, 353, 389
AcsI RAATTY 1 cut(s) 482
AcyI GRCGYC 2 cut(s) 10, 45
AfaI GTAC 2 cut(s) 26, 437
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 5 cut(s) 90, 155, 419, 467, 574
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 2 cut(s) 86, 654
AluI AGCT 2 cut(s) 86, 654
Alw26I GTCTC 2 cut(s) 371, 621
AlwI GGATC 3 cut(s) 327, 353, 389
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 5 cut(s) 77, 83, 118, 121, 124
ApoI RAATTY 1 cut(s) 482
AspLEI GCGC 3 cut(s) 22, 47, 77
AspS9I GGNCC 3 cut(s) 235, 354, 427
AsuHPI GGTGA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 2 cut(s) 354, 427
BanI GGYRCC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 5 cut(s) 64, 95, 130, 133, 136
BccI CCATC 2 cut(s) 117, 240
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 371, 621
BfaI CTAG 3 cut(s) 144, 330, 546
BfoI RGCGCY 2 cut(s) 48, 78
BisI GCNGC 7 cut(s) 43, 78, 81, 84, 119, 122, 125
BlsI GCNGC 7 cut(s) 44, 79, 82, 85, 120, 123, 126
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 2 cut(s) 354, 427
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 3 cut(s) 235, 354, 427
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 247, 269, 514, 523
BmuI ACTGGG 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 634
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 10, 45
BsaJI CCNNGG 3 cut(s) 343, 430, 510
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 238
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 3 cut(s) 343, 430, 510
BseGI GGATG 2 cut(s) 260, 538
BseJI GATNNNNATC 1 cut(s) 192
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 238
BseXI GCAGC 5 cut(s) 64, 95, 130, 133, 136
BshFI GGCC 1 cut(s) 236
BshNI GGYRCC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 371, 621
BsnI GGCC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 179
Bsp143I GATC 4 cut(s) 332, 358, 394, 401
BspACI CCGC 4 cut(s) 42, 63, 69, 80
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 3 cut(s) 327, 353, 389
BspT107I GGYRCC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 3 cut(s) 343, 430, 510
BssMI GATC 4 cut(s) 332, 358, 394, 401
BssNI GRCGYC 2 cut(s) 10, 45
BssT1I CCWWGG 2 cut(s) 430, 510
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 459
BstACI GRCGYC 2 cut(s) 10, 45
BstAPI GCANNNNNTGC 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 14, 18, 31
BstDEI CTNAG 1 cut(s) 528
BstF5I GGATG 2 cut(s) 260, 538
BstH2I RGCGCY 2 cut(s) 48, 78
BstHHI GCGC 3 cut(s) 22, 47, 77
BstKTI GATC 4 cut(s) 335, 361, 397, 404
BstMAI GTCTC 2 cut(s) 371, 621
BstMBI GATC 4 cut(s) 332, 358, 394, 401
BstMWI GCNNNNNNNGC 6 cut(s) 26, 39, 83, 124, 533, 542
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 301, 371
BstSCI CCNGG 1 cut(s) 342
BstV1I GCAGC 5 cut(s) 64, 95, 130, 133, 136
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BsuRI GGCC 1 cut(s) 236
BtsCI GGATG 2 cut(s) 260, 538
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 3 cut(s) 14, 18, 31
CfoI GCGC 3 cut(s) 22, 47, 77
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 3 cut(s) 235, 354, 427
CseI GACGC 1 cut(s) 18
Csp6I GTAC 2 cut(s) 25, 436
CviAII CATG 2 cut(s) 298, 368
CviJI RGCY 6 cut(s) 86, 127, 236, 275, 448, 654
CviKI_1 RGCY 6 cut(s) 86, 127, 236, 275, 448, 654
CviQI GTAC 2 cut(s) 25, 436
DdeI CTNAG 1 cut(s) 528
DinI GGCGCC 1 cut(s) 46
DpnI GATC 4 cut(s) 334, 360, 396, 403
DpnII GATC 4 cut(s) 332, 358, 394, 401
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 2 cut(s) 430, 510
Eco47I GGWCC 2 cut(s) 354, 427
Eco88I CYCGRG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 2 cut(s) 430, 510
EcoT22I ATGCAT 1 cut(s) 262
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 2 cut(s) 430, 510
FaeI CATG 2 cut(s) 301, 371
FaiI YATR 8 cut(s) 57, 211, 299, 369, 414, 425, 585, 595
FatI CATG 2 cut(s) 297, 367
Fnu4HI GCNGC 7 cut(s) 43, 78, 81, 84, 119, 122, 125
FokI GGATG 2 cut(s) 247, 545
Fsp4HI GCNGC 7 cut(s) 43, 78, 81, 84, 119, 122, 125
FspBI CTAG 3 cut(s) 144, 330, 546
GlaI GCGC 3 cut(s) 21, 46, 76
GluI GCNGC 7 cut(s) 43, 78, 81, 84, 119, 122, 125
GsuI CTGGAG 1 cut(s) 494
HaeII RGCGCY 2 cut(s) 48, 78
HaeIII GGCC 1 cut(s) 236
HapII CCGG 1 cut(s) 30
HgaI GACGC 1 cut(s) 18
HhaI GCGC 3 cut(s) 22, 47, 77
Hin1I GRCGYC 2 cut(s) 10, 45
Hin1II CATG 2 cut(s) 301, 371
Hin6I GCGC 3 cut(s) 20, 45, 75
HinP1I GCGC 3 cut(s) 20, 45, 75
HinfI GANTC 3 cut(s) 35, 162, 602
HpaII CCGG 1 cut(s) 30
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 1 cut(s) 358
Hpy188III TCNNGA 2 cut(s) 330, 473
Hpy8I GTNNAC 1 cut(s) 174
HpyAV CCTTC 3 cut(s) 149, 302, 459
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 94, 260, 536
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 39, 83, 124, 533, 542
HpyF3I CTNAG 1 cut(s) 528
Hsp92I GRCGYC 2 cut(s) 10, 45
Hsp92II CATG 2 cut(s) 301, 371
HspAI GCGC 3 cut(s) 20, 45, 75
KasI GGCGCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 332, 358, 394, 401
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 5 cut(s) 64, 95, 130, 133, 136
LweI GCATC 4 cut(s) 247, 269, 514, 523
MaeI CTAG 3 cut(s) 144, 330, 546
MaeIII GTNAC 1 cut(s) 453
MalI GATC 4 cut(s) 334, 360, 396, 403
MboI GATC 4 cut(s) 332, 358, 394, 401
MboII GAAGA 2 cut(s) 213, 431
MflI RGATCY 1 cut(s) 332
MluCI AATT 3 cut(s) 49, 265, 482
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 1 cut(s) 156
MnlI CCTC 9 cut(s) 59, 177, 226, 286, 303, 420, 433, 523, 550
Mph1103I ATGCAT 1 cut(s) 262
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 6 cut(s) 26, 39, 83, 124, 533, 542
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 45
NdeII GATC 4 cut(s) 332, 358, 394, 401
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 2 cut(s) 301, 371
NlaIV GGNNCC 1 cut(s) 46
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 453
NsiI ATGCAT 1 cut(s) 262
NspI RCATGY 2 cut(s) 301, 371
PciI ACATGT 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 35, 602
PkrI GCNGC 7 cut(s) 44, 79, 82, 85, 120, 123, 126
PleI GAGTC 1 cut(s) 156
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 156
PscI ACATGT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 3 cut(s) 235, 354, 427
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 2 cut(s) 26, 437
RsaNI GTAC 2 cut(s) 25, 436
SatI GCNGC 7 cut(s) 43, 78, 81, 84, 119, 122, 125
Sau3AI GATC 4 cut(s) 332, 358, 394, 401
Sau96I GGNCC 3 cut(s) 235, 354, 427
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 344
SetI ASST 8 cut(s) 88, 295, 313, 356, 412, 432, 561, 656
SfaNI GCATC 4 cut(s) 247, 269, 514, 523
SfoI GGCGCC 1 cut(s) 46
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 2 cut(s) 354, 427
SmlI CTYRAG 1 cut(s) 649
SmoI CTYRAG 1 cut(s) 649
Sse9I AATT 3 cut(s) 49, 265, 482
SsiI CCGC 4 cut(s) 42, 63, 69, 80
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 3 cut(s) 144, 330, 546
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 2 cut(s) 430, 510
TaaI ACNGT 1 cut(s) 459
TaqI TCGA 1 cut(s) 165
TasI AATT 3 cut(s) 49, 265, 482
TauI GCSGC 2 cut(s) 45, 83
TfiI GAWTC 2 cut(s) 35, 602
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 5 cut(s) 77, 83, 118, 121, 124
Tsp45I GTSAC 1 cut(s) 453
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 354, 427
XapI RAATTY 1 cut(s) 482
XbaI TCTAGA 1 cut(s) 329
XceI RCATGY 2 cut(s) 301, 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 237
XspI CTAG 3 cut(s) 144, 330, 546
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.