Rw5G030440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011391
Physical Location & Seq
Forward (+)
48289800 .. 48293541
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030440.1

Sequence Viewer

Length: 921 bp
ATGATGAGACGCCAGCAGGCGCAGTACGCCGGCGATTCAGGCGGCGCCAATTCGTATGGTGGCGGAGGCGGTGGCGCTTCGGCAGCTTCAATGCACCACCACCACCACCATCATCAGCAGCAGCAGCCGAGTAGTGTTGGTGCTAGTGATTTTGAAGGACGACTCGAAGCGTTCACACCCGAGAGGGATAGCAATCCTAATCCTAATCCTAATCCATACGCCACTTCTTCCAAACCAGAGGCCCAGTGGAGATGGGAAAGAGATGCATCCAATTCCAAAGCCTCCAATCCATTGACACCTCACATGTTTAGTGAAGGTCAAGTCGGTGATACATCTAGATCCTATTTCCAGGGTCAAAGGTCCGATCCAAAACATGCTTTAGAGACACAGAGCAACAACGATCCCCGATCTCAACCTCATATTGAAGATATGGACCTTGGGTACGAGGATAAGCCTTCGTCACAGTCCTTTGAAGTTCTGGAGCAGAAATTCCTTGATGACATTAGGAAACTAACCAAGGAACAGAATGATGCTGAGGATGCAGAAAATGCTAGACACAGAGAGAAAATTGGTACAATCAATGCTCAGTATGAAGAACAATTAGCATCACTACGGTGCCAGCATGCTGGCCGTAGAGATGATTTACTTCGGAGGGAATCAAATGCCCGGCAGCATCAATACCAGCAATCCATGATGGATCGTTACCCTAAAAGCAGCATGGGCTCCTCCGATCTTCATGGTTACAGTGGAATTGCAGCCTCAGCTACTGCTGGCGATACACGCAGAGGCTACGAAACTGACCAATATGATTCTTACAGAGAGCGGGCTCGATTTCTTGGGGGCTCCCGTGATCATGGGTTTGAGCCTAGGGGACCATATCCGGGAGGGCGTGTTTATGATACTGGCTCACGTTATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

34.42

Weight (kDa)

6.1

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011391)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22040 AT5G22040 AT5G22040
fragaria_vesca FvH4_3g26150 FvH4_3g26150
malus_domestica MD03G1159500.v1.1 MD11G1174800.v1.1
prunus_persica Prupe.6G147200_v2.0.a1
pyrus_communis pycom03g11210 pycom11g14950
rosa_chinensis RchiOBHm_Chr5g0049091
rosa_laevigata RLG00000034588
rosa_multiflora Rmu_co8392037.1_g000001
rosa_roxburghii Rroxscaffold_1G00032780
rosa_rugosa Rorug05G0243100
rosa_samantha Rh5AG322600 Rh5BG333200 Rh5CG358100 Rh5DG344600
rosa_wichuraiana Rw5G030440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 44, 615
AccBSI CCGCTC 1 cut(s) 823
AciI CCGC 4 cut(s) 42, 63, 69, 823
AclWI GGATC 4 cut(s) 333, 359, 395, 705
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 488
AcyI GRCGYC 2 cut(s) 10, 45
AfaI GTAC 3 cut(s) 26, 443, 574
AfiI CCNNNNNNNGG 1 cut(s) 881
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 90, 155, 425, 473
AjnI CCWGG 1 cut(s) 348
AleI CACNNNNGTG 1 cut(s) 613
AluBI AGCT 2 cut(s) 86, 764
AluI AGCT 2 cut(s) 86, 764
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 4 cut(s) 333, 359, 395, 705
AlwNI CAGNNNCTG 1 cut(s) 767
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 2 cut(s) 240, 628
ApeKI GCWGC 7 cut(s) 83, 118, 121, 124, 670, 714, 755
ApoI RAATTY 1 cut(s) 488
AspA2I CCTAGG 1 cut(s) 866
AspLEI GCGC 3 cut(s) 22, 47, 77
AspS9I GGNCC 4 cut(s) 241, 360, 433, 872
AsuC2I CCSGG 2 cut(s) 667, 882
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 3 cut(s) 360, 433, 872
AvrII CCTAGG 1 cut(s) 866
BanI GGYRCC 2 cut(s) 44, 615
BanII GRGCYC 3 cut(s) 725, 829, 845
BbvCI CCTCAGC 2 cut(s) 534, 760
BbvI GCAGC 7 cut(s) 95, 130, 133, 136, 682, 726, 767
BccI CCATC 3 cut(s) 117, 246, 688
BceAI ACGGC 1 cut(s) 615
BciT130I CCWGG 1 cut(s) 350
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 2 cut(s) 667, 882
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 4 cut(s) 144, 336, 552, 867
BfoI RGCGCY 2 cut(s) 48, 78
BisI GCNGC 8 cut(s) 43, 84, 119, 122, 125, 671, 715, 756
BlnI CCTAGG 1 cut(s) 866
BlsI GCNGC 8 cut(s) 44, 85, 120, 123, 126, 672, 716, 757
Bme1390I CCNGG 3 cut(s) 350, 667, 882
Bme18I GGWCC 3 cut(s) 360, 433, 872
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 4 cut(s) 241, 360, 433, 872
BmiI GGNNCC 5 cut(s) 46, 617, 724, 844, 873
BmrFI CCNGG 3 cut(s) 350, 667, 882
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 6 cut(s) 253, 275, 520, 529, 614, 682
BmuI ACTGGG 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 2 cut(s) 534, 760
BpuMI CCSGG 2 cut(s) 667, 882
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 10, 45
BsaJI CCNNGG 4 cut(s) 349, 436, 516, 866
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 1 cut(s) 881
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 244, 907
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 4 cut(s) 349, 436, 516, 866
BseGI GGATG 2 cut(s) 266, 544
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 881
BseMII CTCAG 3 cut(s) 525, 599, 774
BseNI ACTGG 2 cut(s) 244, 907
BseRI GAGGAG 1 cut(s) 715
BseXI GCAGC 7 cut(s) 95, 130, 133, 136, 682, 726, 767
BshFI GGCC 2 cut(s) 242, 630
BshNI GGYRCC 2 cut(s) 44, 615
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 3 cut(s) 30, 667, 881
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 1 cut(s) 881
BsmAI GTCTC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 885
BsnI GGCC 2 cut(s) 242, 630
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 725, 829, 845
Bsp143I GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
BspACI CCGC 4 cut(s) 42, 63, 69, 823
BspANI GGCC 2 cut(s) 242, 630
BspCNI CTCAG 3 cut(s) 526, 598, 773
BspLI GGNNCC 5 cut(s) 46, 617, 724, 844, 873
BspPI GGATC 4 cut(s) 333, 359, 395, 705
BspT107I GGYRCC 2 cut(s) 44, 615
BsrBI CCGCTC 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 2 cut(s) 244, 907
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 4 cut(s) 349, 436, 516, 866
BssMI GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
BssNI GRCGYC 2 cut(s) 10, 45
BssT1I CCWWGG 3 cut(s) 436, 516, 866
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 3 cut(s) 465, 615, 746
BstACI GRCGYC 2 cut(s) 10, 45
BstAPI GCANNNNNTGC 1 cut(s) 548
BstC8I GCNNGC 8 cut(s) 14, 18, 31, 620, 624, 628, 772, 825
BstDEI CTNAG 3 cut(s) 534, 585, 760
BstF5I GGATG 2 cut(s) 266, 544
BstH2I RGCGCY 2 cut(s) 48, 78
BstHHI GCGC 3 cut(s) 22, 47, 77
BstKTI GATC 7 cut(s) 341, 367, 403, 410, 700, 733, 853
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
BstMWI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 539, 548, 720, 761, 780
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 3 cut(s) 307, 377, 626
BstSCI CCNGG 3 cut(s) 348, 665, 880
BstV1I GCAGC 7 cut(s) 95, 130, 133, 136, 682, 726, 767
BstX2I RGATCY 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 1 cut(s) 338
BsuRI GGCC 2 cut(s) 242, 630
BtsCI GGATG 2 cut(s) 266, 544
BtsIMutI CAGTG 2 cut(s) 251, 751
Cac8I GCNNGC 8 cut(s) 14, 18, 31, 620, 624, 628, 772, 825
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 3 cut(s) 22, 47, 77
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 241, 360, 433, 872
CseI GACGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 25, 442, 573
CviAII CATG 7 cut(s) 304, 374, 623, 691, 718, 737, 854
CviQI GTAC 3 cut(s) 25, 442, 573
DdeI CTNAG 3 cut(s) 534, 585, 760
DinI GGCGCC 1 cut(s) 46
DpnI GATC 7 cut(s) 340, 366, 402, 409, 699, 732, 852
DpnII GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
EaeI YGGCCR 1 cut(s) 628
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 3 cut(s) 436, 516, 866
Eco24I GRGCYC 3 cut(s) 725, 829, 845
Eco47I GGWCC 3 cut(s) 360, 433, 872
Eco88I CYCGRG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 3 cut(s) 436, 516, 866
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 3 cut(s) 725, 829, 845
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 3 cut(s) 436, 516, 866
FaeI CATG 7 cut(s) 307, 377, 626, 694, 721, 740, 857
FaqI GGGAC 1 cut(s) 885
FatI CATG 7 cut(s) 303, 373, 622, 690, 717, 736, 853
FauI CCCGC 1 cut(s) 816
FbaI TGATCA 1 cut(s) 850
Fnu4HI GCNGC 8 cut(s) 43, 84, 119, 122, 125, 671, 715, 756
FokI GGATG 2 cut(s) 253, 551
FriOI GRGCYC 3 cut(s) 725, 829, 845
Fsp4HI GCNGC 8 cut(s) 43, 84, 119, 122, 125, 671, 715, 756
FspBI CTAG 4 cut(s) 144, 336, 552, 867
GlaI GCGC 3 cut(s) 21, 46, 76
GluI GCNGC 8 cut(s) 43, 84, 119, 122, 125, 671, 715, 756
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 2 cut(s) 48, 78
HaeIII GGCC 2 cut(s) 242, 630
HapII CCGG 3 cut(s) 30, 667, 881
HgaI GACGC 1 cut(s) 18
HhaI GCGC 3 cut(s) 22, 47, 77
Hin1I GRCGYC 2 cut(s) 10, 45
Hin1II CATG 7 cut(s) 307, 377, 626, 694, 721, 740, 857
Hin6I GCGC 3 cut(s) 20, 45, 75
HinP1I GCGC 3 cut(s) 20, 45, 75
HinfI GANTC 4 cut(s) 35, 162, 656, 809
HpaII CCGG 3 cut(s) 30, 667, 881
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 3 cut(s) 364, 651, 730
Hpy188III TCNNGA 2 cut(s) 336, 479
Hpy8I GTNNAC 1 cut(s) 174
HpyAV CCTTC 3 cut(s) 149, 308, 465
HpyCH4III ACNGT 3 cut(s) 465, 615, 746
HpyCH4IV ACGT 1 cut(s) 910
HpyCH4V TGCA 4 cut(s) 94, 266, 542, 755
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 539, 548, 720, 761, 780
HpyF3I CTNAG 3 cut(s) 534, 585, 760
HpySE526I ACGT 1 cut(s) 910
Hsp92I GRCGYC 2 cut(s) 10, 45
Hsp92II CATG 7 cut(s) 307, 377, 626, 694, 721, 740, 857
HspAI GCGC 3 cut(s) 20, 45, 75
KasI GGCGCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
LmnI GCTCC 3 cut(s) 481, 728, 848
Lsp1109I GCAGC 7 cut(s) 95, 130, 133, 136, 682, 726, 767
LweI GCATC 6 cut(s) 253, 275, 520, 529, 614, 682
MaeI CTAG 4 cut(s) 144, 336, 552, 867
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 3 cut(s) 459, 701, 740
MalI GATC 7 cut(s) 340, 366, 402, 409, 699, 732, 852
MbiI CCGCTC 1 cut(s) 823
MboI GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
MboII GAAGA 4 cut(s) 219, 437, 605, 725
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 3 cut(s) 725, 829, 845
MluCI AATT 6 cut(s) 49, 271, 488, 567, 599, 750
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 1 cut(s) 156
Mph1103I ATGCAT 1 cut(s) 268
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 613
MspI CCGG 3 cut(s) 30, 667, 881
MspR9I CCNGG 3 cut(s) 350, 667, 882
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 9 cut(s) 26, 39, 83, 124, 539, 548, 720, 761, 780
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 2 cut(s) 667, 882
NdeII GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 7 cut(s) 307, 377, 626, 694, 721, 740, 857
NlaIV GGNNCC 5 cut(s) 46, 617, 724, 844, 873
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 3 cut(s) 307, 377, 626
OliI CACNNNNGTG 1 cut(s) 613
PaeI GCATGC 1 cut(s) 626
PciI ACATGT 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 3 cut(s) 35, 656, 809
PfoI TCCNGGA 1 cut(s) 880
PkrI GCNGC 8 cut(s) 44, 85, 120, 123, 126, 672, 716, 757
PleI GAGTC 1 cut(s) 156
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 156
PscI ACATGT 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 5 cut(s) 46, 617, 724, 844, 873
PspPI GGNCC 4 cut(s) 241, 360, 433, 872
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 3 cut(s) 26, 443, 574
RsaNI GTAC 3 cut(s) 25, 442, 573
RseI CAYNNNNRTG 1 cut(s) 613
SatI GCNGC 8 cut(s) 43, 84, 119, 122, 125, 671, 715, 756
Sau3AI GATC 7 cut(s) 338, 364, 400, 407, 697, 730, 850
Sau96I GGNCC 4 cut(s) 241, 360, 433, 872
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 350, 667, 882
SduI GDGCHC 3 cut(s) 725, 829, 845
SetI ASST 8 cut(s) 88, 301, 319, 362, 418, 438, 766, 913
SfaNI GCATC 6 cut(s) 253, 275, 520, 529, 614, 682
SfoI GGCGCC 1 cut(s) 46
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 3 cut(s) 360, 433, 872
SmiMI CAYNNNNRTG 1 cut(s) 613
SphI GCATGC 1 cut(s) 626
Sse9I AATT 6 cut(s) 49, 271, 488, 567, 599, 750
SsiI CCGC 4 cut(s) 42, 63, 69, 823
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 4 cut(s) 144, 336, 552, 867
StyD4I CCNGG 3 cut(s) 348, 665, 880
StyI CCWWGG 3 cut(s) 436, 516, 866
TaaI ACNGT 3 cut(s) 465, 615, 746
TaiI ACGT 1 cut(s) 913
TaqI TCGA 2 cut(s) 165, 829
TasI AATT 6 cut(s) 49, 271, 488, 567, 599, 750
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 35, 656, 809
TscAI CASTG 2 cut(s) 251, 751
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 7 cut(s) 83, 118, 121, 124, 670, 714, 755
Tsp45I GTSAC 1 cut(s) 459
TspDTI ATGAA 2 cut(s) 606, 725
TspRI CASTG 2 cut(s) 251, 751
VpaK11BI GGWCC 3 cut(s) 360, 433, 872
XapI RAATTY 1 cut(s) 488
XbaI TCTAGA 1 cut(s) 335
XceI RCATGY 3 cut(s) 307, 377, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 243
XmaJI CCTAGG 1 cut(s) 866
XspI CTAG 4 cut(s) 144, 336, 552, 867
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.