MD03G1189900.v1.1

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008535
Physical Location & Seq
Forward (+)
26083498 .. 26085984
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1189900.v1.1.491

Sequence Viewer

Length: 633 bp
ATGGCGACGACGTCGGTTGATTCTCCAAACACTGAGATGGACACCAACACTGAGCTCGATACCAATCCAAACCCTAATTCGAACCCTAATTCTTCGAACTCATTAGTCCCTGCGTCCCCCAATGCCCCTGCTGTCTGCCTCCTCAGGTTCGCCGGAGACTCCGCTGGCGGCGCGTTCATGGGCTCCGTTTTTGGATATGGTGCGGGGTTGGTCAAGAAAAAAGGCTTTAAAGGATCCTTTGGAGAGGCAGGATCTTATGCTAAGACATTTGCAGTTTTGTCCGGAGTGCACAGTTTGGTTGTCTGCATTTTAAAGAGGCTGCGAGGAAAGGATGATGTTATTAATGCTGGAGTAGCTGGCTGTTGCACTGGTCTTGCTCTTAGTTTTCCAGGTGCACCGCAGGCTCTTCTACAAAGCTGTCTCACTTTTGGGGCATTCTCATTTGTTATTGAAGGCCTAAACAAGCAGCAGCCAGCTTTAGCGCAACCATTTTCTGTGAGGAAGAAAAGTGAGCAAGTCTCTCCCCTGGTCCTCCCCCTTCAACTTTCACTTCCGGGCGAACTAAAAGCAGGTTTCACTTCCTTTTGCAATTCCTTGAAGAATCGCAAGAAAGGCAGTTCTCACACAGCCTAG

Protein Analysis

211

Amino Acids

21.7

Weight (kDa)

9.22

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 53 - 147 2.1e-14 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 560
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 281
AciI CCGC 4 cut(s) 162, 168, 203, 398
AclWI GGATC 3 cut(s) 228, 241, 259
AcyI GRCGYC 1 cut(s) 11
AgsI TTSAA 3 cut(s) 452, 542, 598
AjnI CCWGG 2 cut(s) 388, 525
AjuI GAANNNNNNNTTGG 2 cut(s) 61, 93
AluBI AGCT 4 cut(s) 55, 356, 417, 476
AluI AGCT 4 cut(s) 55, 356, 417, 476
Alw21I GWGCWC 3 cut(s) 57, 291, 397
Alw26I GTCTC 3 cut(s) 150, 425, 523
Alw44I GTGCAC 2 cut(s) 287, 393
AlwI GGATC 3 cut(s) 228, 241, 259
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 1 cut(s) 454
ApaLI GTGCAC 2 cut(s) 287, 393
ApeKI GCWGC 3 cut(s) 319, 466, 469
AseI ATTAAT 1 cut(s) 342
AspLEI GCGC 2 cut(s) 173, 484
AspS9I GGNCC 1 cut(s) 529
AsuC2I CCSGG 1 cut(s) 555
AsuII TTCGAA 2 cut(s) 80, 95
AvaII GGWCC 1 cut(s) 529
AxyI CCTNAGG 1 cut(s) 143
BaeGI GKGCMC 2 cut(s) 291, 397
BamHI GGATCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 57, 185
Bbv12I GWGCWC 3 cut(s) 57, 291, 397
BbvI GCAGC 3 cut(s) 306, 478, 481
BccI CCATC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 390, 527
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 3 cut(s) 150, 425, 523
BfaI CTAG 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 560
BisI GCNGC 4 cut(s) 169, 320, 467, 470
BlsI GCNGC 4 cut(s) 170, 321, 468, 471
Bme1390I CCNGG 3 cut(s) 390, 527, 555
Bme18I GGWCC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 2 cut(s) 184, 235
BmrFI CCNGG 3 cut(s) 390, 527, 555
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 1 cut(s) 369
Bpu14I TTCGAA 2 cut(s) 80, 95
BpuMI CCSGG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 525
BsaWI WCCGGW 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 373
Bse21I CCTNAGG 1 cut(s) 143
Bse8I GATNNNNATC 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 2 cut(s) 390, 527
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 63
BseMII CTCAG 3 cut(s) 24, 42, 157
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 2 cut(s) 291, 397
BseXI GCAGC 3 cut(s) 306, 478, 481
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 456
BsiHKAI GWGCWC 3 cut(s) 57, 291, 397
BsiSI CCGG 3 cut(s) 153, 282, 554
BslFI GGGAC 2 cut(s) 92, 100
BsmAI GTCTC 3 cut(s) 150, 425, 523
BsmFI GGGAC 2 cut(s) 92, 100
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 1 cut(s) 456
Bsp119I TTCGAA 2 cut(s) 80, 95
Bsp1286I GDGCHC 4 cut(s) 57, 185, 291, 397
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 2 cut(s) 233, 251
BspACI CCGC 4 cut(s) 162, 168, 203, 398
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 3 cut(s) 25, 43, 156
BspEI TCCGGA 1 cut(s) 281
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 184, 235
BspMI ACCTGC 1 cut(s) 560
BspPI GGATC 3 cut(s) 228, 241, 259
BspQI GCTCTTC 1 cut(s) 411
BspT104I TTCGAA 2 cut(s) 80, 95
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 2 cut(s) 233, 251
BssNI GRCGYC 1 cut(s) 11
Bst2UI CCWGG 2 cut(s) 390, 527
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 11
BstBI TTCGAA 2 cut(s) 80, 95
BstC8I GCNNGC 4 cut(s) 166, 358, 402, 474
BstDEI CTNAG 5 cut(s) 33, 51, 143, 261, 380
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 2 cut(s) 173, 484
BstKTI GATC 2 cut(s) 236, 254
BstMAI GTCTC 3 cut(s) 150, 425, 523
BstMBI GATC 2 cut(s) 233, 251
BstMWI GCNNNNNNNGC 4 cut(s) 170, 353, 401, 612
BstNI CCWGG 2 cut(s) 390, 527
BstSCI CCNGG 3 cut(s) 388, 525, 553
BstSLI GKGCMC 2 cut(s) 291, 397
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 306, 478, 481
BstX2I RGATCY 2 cut(s) 233, 251
BstYI RGATCY 2 cut(s) 233, 251
Bsu36I CCTNAGG 1 cut(s) 143
BsuRI GGCC 1 cut(s) 456
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 30, 48, 366
BveI ACCTGC 1 cut(s) 560
Cac8I GCNNGC 4 cut(s) 166, 358, 402, 474
CfoI GCGC 2 cut(s) 173, 484
Cfr13I GGNCC 1 cut(s) 529
CseI GACGC 1 cut(s) 102
CviAII CATG 1 cut(s) 178
DdeI CTNAG 5 cut(s) 33, 51, 143, 261, 380
DpnI GATC 2 cut(s) 235, 253
DpnII GATC 2 cut(s) 233, 251
DraI TTTAAA 2 cut(s) 229, 312
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 55
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 57, 185
Eco47I GGWCC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 55
Eco81I CCTNAGG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 388, 525
EcoT38I GRGCYC 2 cut(s) 57, 185
FaeI CATG 1 cut(s) 181
FaiI YATR 3 cut(s) 179, 198, 258
FaqI GGGAC 2 cut(s) 92, 100
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 196
Fnu4HI GCNGC 4 cut(s) 169, 320, 467, 470
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 2 cut(s) 57, 185
Fsp4HI GCNGC 4 cut(s) 169, 320, 467, 470
FspBI CTAG 1 cut(s) 631
GlaI GCGC 2 cut(s) 172, 483
GluI GCNGC 4 cut(s) 169, 320, 467, 470
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 1 cut(s) 456
HapII CCGG 3 cut(s) 153, 282, 554
HgaI GACGC 1 cut(s) 102
HhaI GCGC 2 cut(s) 173, 484
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 2 cut(s) 171, 482
HinP1I GCGC 2 cut(s) 171, 482
HinfI GANTC 3 cut(s) 20, 158, 601
HpaII CCGG 3 cut(s) 153, 282, 554
Hpy166II GTNNAC 2 cut(s) 289, 395
Hpy188III TCNNGA 2 cut(s) 214, 282
Hpy8I GTNNAC 2 cut(s) 289, 395
Hpy99I CGWCG 3 cut(s) 10, 13, 16
HpyAV CCTTC 2 cut(s) 446, 548
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 6 cut(s) 272, 289, 306, 366, 395, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 353, 401, 612
HpyF3I CTNAG 5 cut(s) 33, 51, 143, 261, 380
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 2 cut(s) 171, 482
Kpn2I TCCGGA 1 cut(s) 281
Kzo9I GATC 2 cut(s) 233, 251
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 3 cut(s) 306, 478, 481
MaeI CTAG 1 cut(s) 631
MaeII ACGT 1 cut(s) 11
MalI GATC 2 cut(s) 235, 253
MboI GATC 2 cut(s) 233, 251
MboII GAAGA 4 cut(s) 84, 398, 514, 610
MflI RGATCY 2 cut(s) 233, 251
MhlI GDGCHC 4 cut(s) 57, 185, 291, 397
MluCI AATT 3 cut(s) 76, 88, 589
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 7 cut(s) 149, 152, 238, 309, 317, 492, 542
MroI TCCGGA 1 cut(s) 281
MseI TTAA 3 cut(s) 228, 311, 342
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 3 cut(s) 153, 282, 554
MspR9I CCNGG 3 cut(s) 390, 527, 555
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 2 cut(s) 390, 527
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 4 cut(s) 170, 353, 401, 612
NciI CCSGG 1 cut(s) 555
NdeII GATC 2 cut(s) 233, 251
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 2 cut(s) 184, 235
NspV TTCGAA 2 cut(s) 80, 95
PceI AGGCCT 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 411
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 2 cut(s) 20, 601
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 4 cut(s) 170, 321, 468, 471
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 2 cut(s) 388, 525
PspGI CCWGG 2 cut(s) 388, 525
PspN4I GGNNCC 2 cut(s) 184, 235
PspPI GGNCC 1 cut(s) 529
PsuI RGATCY 2 cut(s) 233, 251
PsyI GACNNNGTC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 35
SacI GAGCTC 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 228, 311, 342
SatI GCNGC 4 cut(s) 169, 320, 467, 470
Sau3AI GATC 2 cut(s) 233, 251
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 390, 527, 555
SduI GDGCHC 4 cut(s) 57, 185, 291, 397
SetI ASST 8 cut(s) 14, 57, 149, 358, 394, 419, 478, 574
SfuI TTCGAA 2 cut(s) 80, 95
SinI GGWCC 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 3 cut(s) 76, 88, 589
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 4 cut(s) 162, 168, 203, 398
SspMI CTAG 1 cut(s) 631
SstI GAGCTC 1 cut(s) 57
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 388, 525, 553
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 57, 80, 95
TasI AATT 3 cut(s) 76, 88, 589
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 2 cut(s) 20, 601
Tru1I TTAA 3 cut(s) 228, 311, 342
Tru9I TTAA 3 cut(s) 228, 311, 342
TscAI CASTG 3 cut(s) 37, 55, 373
TseI GCWGC 3 cut(s) 319, 466, 469
TspDTI ATGAA 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 3 cut(s) 37, 55, 373
Tth111I GACNNNGTC 1 cut(s) 10
VneI GTGCAC 2 cut(s) 287, 393
VpaK11BI GGWCC 1 cut(s) 529
VspI ATTAAT 1 cut(s) 342
XspI CTAG 1 cut(s) 631
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.