pycom11g17900

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008535
Physical Location & Seq
Forward (+)
20212757 .. 20214662
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g17900.1

Sequence Viewer

Length: 621 bp
ATGGCGACGACGTCGGTTGATTCTCCAAACACTGAGATGGATCCCAACACTGAGCTTGATACTAATCCTAACCCTAATTCTTCAAACTCCTTAGTCCCTGCCTCCCCCAGTGGCCCTGCTGTCTGCCTTCTCAGGTTCGCCGGAGACTCCGCTGGTGGCGCGTTCATGGGCTCCATTTTTGGATACGGTGCGGGGTTGTTCAAGAAAAAAGGTTTTAAAGGATCATTTGCAGATGCAGGATCTTCTGCAAAGACATTCGCAGTTTTGTCCGGAGTGCACAGTTTGGTTGTCTGCATGTTGAAGAGGCTGCGAGGAAAGGATGATGTCATTAATGCTGGAGTAGCTGGATGTTGCACTGGTCTTGCTCTTAGTTTTCCAGGTGCACCCCAGGCTCTTCTACAAAGCTGTCTCACTTTTGGGGCATTCTCATTTGTTATTGAAGGCCTAAACAAGCAGCAGCCGGCTTTAGCACAACCATTTTCTGTGAGGAAGAAAAGTGAGCAAGTTCGTCCCTTGGTGCTCCCCCTTCAATTTTCACTCCCGGGAGAACTAAAAGCGGGTTTCACCTTCTTTTGCAATTCCTTGAAGAATCGCGAGAAGGGCACTTCTCACCCAGCCTAA

Protein Analysis

207

Amino Acids

21.42

Weight (kDa)

9.11

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 49 - 143 5e-14 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
AccII CGCG 2 cut(s) 161, 594
AccIII TCCGGA 1 cut(s) 269
AciI CCGC 3 cut(s) 150, 191, 557
AclWI GGATC 4 cut(s) 35, 48, 229, 247
AcyI GRCGYC 1 cut(s) 11
AgsI TTSAA 6 cut(s) 84, 202, 301, 440, 530, 586
AjnI CCWGG 2 cut(s) 376, 387
AluBI AGCT 3 cut(s) 55, 344, 405
AluI AGCT 3 cut(s) 55, 344, 405
Alw21I GWGCWC 3 cut(s) 279, 385, 522
Alw26I GTCTC 2 cut(s) 138, 413
Alw44I GTGCAC 2 cut(s) 275, 381
AlwI GGATC 4 cut(s) 35, 48, 229, 247
Ama87I CYCGRG 1 cut(s) 541
Aor13HI TCCGGA 1 cut(s) 269
AoxI GGCC 2 cut(s) 112, 442
ApaLI GTGCAC 2 cut(s) 275, 381
ApeKI GCWGC 3 cut(s) 307, 454, 457
AseI ATTAAT 1 cut(s) 330
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 113
AsuC2I CCSGG 2 cut(s) 542, 543
AsuHPI GGTGA 2 cut(s) 556, 602
AvaI CYCGRG 1 cut(s) 541
BaeGI GKGCMC 3 cut(s) 279, 385, 605
BamHI GGATCC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 173
Bbv12I GWGCWC 3 cut(s) 279, 385, 522
BbvI GCAGC 3 cut(s) 294, 466, 469
BccI CCATC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 378, 389
BciVI GTATCC 1 cut(s) 176
BcnI CCSGG 2 cut(s) 542, 543
BcoDI GTCTC 2 cut(s) 138, 413
BfuI GTATCC 1 cut(s) 176
BisI GCNGC 3 cut(s) 308, 455, 458
BlsI GCNGC 3 cut(s) 309, 456, 459
Bme1390I CCNGG 4 cut(s) 378, 389, 542, 543
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 2 cut(s) 42, 172
BmrFI CCNGG 4 cut(s) 378, 389, 542, 543
BmrI ACTGGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 357
BpuMI CCSGG 2 cut(s) 542, 543
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 3 cut(s) 387, 513, 541
BsaWI WCCGGW 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 108, 361
Bse8I GATNNNNATC 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 269
BseBI CCWGG 2 cut(s) 378, 389
BseDI CCNNGG 3 cut(s) 387, 513, 541
BseGI GGATG 2 cut(s) 325, 353
BseJI GATNNNNATC 1 cut(s) 63
BseMII CTCAG 3 cut(s) 24, 42, 145
BseNI ACTGG 2 cut(s) 108, 361
BseSI GKGCMC 3 cut(s) 279, 385, 605
BseXI GCAGC 3 cut(s) 294, 466, 469
BseYI CCCAGC 1 cut(s) 613
Bsh1236I CGCG 2 cut(s) 161, 594
BshFI GGCC 2 cut(s) 114, 444
BsiHKAI GWGCWC 3 cut(s) 279, 385, 522
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 4 cut(s) 141, 270, 461, 542
BslFI GGGAC 2 cut(s) 80, 495
BsmAI GTCTC 2 cut(s) 138, 413
BsmFI GGGAC 2 cut(s) 80, 495
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 2 cut(s) 114, 444
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 5 cut(s) 173, 279, 385, 522, 605
Bsp13I TCCGGA 1 cut(s) 269
Bsp143I GATC 3 cut(s) 40, 221, 239
Bsp68I TCGCGA 1 cut(s) 594
BspACI CCGC 3 cut(s) 150, 191, 557
BspANI GGCC 2 cut(s) 114, 444
BspCNI CTCAG 3 cut(s) 25, 43, 144
BspEI TCCGGA 1 cut(s) 269
BspFNI CGCG 2 cut(s) 161, 594
BspLI GGNNCC 2 cut(s) 42, 172
BspPI GGATC 4 cut(s) 35, 48, 229, 247
BspQI GCTCTTC 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 108, 361
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 387, 513, 541
BssMI GATC 3 cut(s) 40, 221, 239
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 378, 389
Bst4CI ACNGT 2 cut(s) 188, 281
Bst6I CTCTTC 2 cut(s) 296, 399
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 5 cut(s) 33, 51, 91, 131, 368
BstF5I GGATG 2 cut(s) 325, 353
BstFNI CGCG 2 cut(s) 161, 594
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 43, 224, 242
BstMAI GTCTC 2 cut(s) 138, 413
BstMBI GATC 3 cut(s) 40, 221, 239
BstMWI GCNNNNNNNGC 4 cut(s) 158, 341, 389, 600
BstNI CCWGG 2 cut(s) 378, 389
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 4 cut(s) 376, 387, 540, 541
BstSLI GKGCMC 3 cut(s) 279, 385, 605
BstUI CGCG 2 cut(s) 161, 594
BstV1I GCAGC 3 cut(s) 294, 466, 469
BstX2I RGATCY 2 cut(s) 40, 239
BstYI RGATCY 2 cut(s) 40, 239
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 2 cut(s) 114, 444
BtsCI GGATG 2 cut(s) 325, 353
BtsIMutI CAGTG 4 cut(s) 30, 48, 115, 354
BtuMI TCGCGA 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 462
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 113
Cfr9I CCCGGG 1 cut(s) 541
CviAII CATG 2 cut(s) 166, 295
DdeI CTNAG 5 cut(s) 33, 51, 91, 131, 368
DpnI GATC 3 cut(s) 42, 223, 241
DpnII GATC 3 cut(s) 40, 221, 239
DraI TTTAAA 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 296, 399
EarI CTCTTC 2 cut(s) 296, 399
Eco130I CCWWGG 1 cut(s) 513
Eco147I AGGCCT 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 376, 387
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 169, 298
FaiI YATR 2 cut(s) 167, 296
FaqI GGGAC 2 cut(s) 80, 495
FatI CATG 2 cut(s) 165, 294
FauI CCCGC 2 cut(s) 184, 550
Fnu4HI GCNGC 3 cut(s) 308, 455, 458
FokI GGATG 2 cut(s) 332, 360
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 3 cut(s) 308, 455, 458
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 308, 455, 458
GsaI CCCAGC 1 cut(s) 617
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 2 cut(s) 114, 444
HapII CCGG 4 cut(s) 141, 270, 461, 542
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 2 cut(s) 169, 298
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 3 cut(s) 20, 146, 589
HpaII CCGG 4 cut(s) 141, 270, 461, 542
HphI GGTGA 2 cut(s) 556, 602
Hpy166II GTNNAC 2 cut(s) 277, 383
Hpy188III TCNNGA 3 cut(s) 202, 270, 593
Hpy8I GTNNAC 2 cut(s) 277, 383
Hpy99I CGWCG 3 cut(s) 10, 13, 16
HpyAV CCTTC 5 cut(s) 137, 434, 536, 577, 592
HpyCH4III ACNGT 2 cut(s) 188, 281
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 8 cut(s) 230, 236, 248, 277, 294, 354, 383, 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 341, 389, 600
HpyF3I CTNAG 5 cut(s) 33, 51, 91, 131, 368
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 2 cut(s) 169, 298
HspAI GCGC 1 cut(s) 159
Kpn2I TCCGGA 1 cut(s) 269
KroI GCCGGC 1 cut(s) 460
KroNI GCCGGC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 40, 221, 239
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 176, 525
Lsp1109I GCAGC 3 cut(s) 294, 466, 469
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 11
MalI GATC 3 cut(s) 42, 223, 241
MboI GATC 3 cut(s) 40, 221, 239
MboII GAAGA 6 cut(s) 72, 234, 313, 386, 502, 598
MflI RGATCY 2 cut(s) 40, 239
MhlI GDGCHC 5 cut(s) 173, 279, 385, 522, 605
MluCI AATT 3 cut(s) 76, 530, 577
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 4 cut(s) 112, 297, 305, 480
MroI TCCGGA 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 460
MseI TTAA 2 cut(s) 216, 330
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 4 cut(s) 141, 270, 461, 542
MspR9I CCNGG 4 cut(s) 378, 389, 542, 543
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 2 cut(s) 378, 389
MvnI CGCG 2 cut(s) 161, 594
MwoI GCNNNNNNNGC 4 cut(s) 158, 341, 389, 600
NaeI GCCGGC 1 cut(s) 462
NciI CCSGG 2 cut(s) 542, 543
NdeII GATC 3 cut(s) 40, 221, 239
NgoMIV GCCGGC 1 cut(s) 460
NlaIII CATG 2 cut(s) 169, 298
NlaIV GGNNCC 2 cut(s) 42, 172
NruI TCGCGA 1 cut(s) 594
NspI RCATGY 1 cut(s) 298
PceI AGGCCT 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 399
PctI GAATGC 1 cut(s) 422
PdiI GCCGGC 1 cut(s) 462
PfeI GAWTC 2 cut(s) 20, 589
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 3 cut(s) 309, 456, 459
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PshBI ATTAAT 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 376, 387
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 376, 387
PspN4I GGNNCC 2 cut(s) 42, 172
PspPI GGNCC 1 cut(s) 113
PsuI RGATCY 2 cut(s) 40, 239
PsyI GACNNNGTC 1 cut(s) 10
RruI TCGCGA 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 2 cut(s) 216, 330
SatI GCNGC 3 cut(s) 308, 455, 458
Sau3AI GATC 3 cut(s) 40, 221, 239
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 4 cut(s) 378, 389, 542, 543
SduI GDGCHC 5 cut(s) 173, 279, 385, 522, 605
SetI ASST 8 cut(s) 14, 57, 137, 214, 346, 382, 407, 569
SfaNI GCATC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 35
Sse9I AATT 3 cut(s) 76, 530, 577
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 3 cut(s) 150, 191, 557
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 4 cut(s) 376, 387, 540, 541
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 188, 281
TaiI ACGT 1 cut(s) 14
TasI AATT 3 cut(s) 76, 530, 577
TfiI GAWTC 2 cut(s) 20, 589
Tru1I TTAA 2 cut(s) 216, 330
Tru9I TTAA 2 cut(s) 216, 330
TscAI CASTG 4 cut(s) 37, 55, 115, 361
TseI GCWGC 3 cut(s) 307, 454, 457
TspDTI ATGAA 1 cut(s) 154
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 4 cut(s) 37, 55, 115, 361
Tth111I GACNNNGTC 1 cut(s) 10
VneI GTGCAC 2 cut(s) 275, 381
VspI ATTAAT 1 cut(s) 330
XceI RCATGY 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 541
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.