Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002572

Physical Location & Seq

Genomic Coordinates Reverse (-)

31148026 .. 31151074

Gene Structure 3049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1226000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 789 bp

ATGATGGAACGCCTCGCTATGGAACAGAACTTCTATGAGCCTGAGACACGCTTTGTTTCAGTTGGAGATGTTTCGGTCAAGAAGTGGAATTCAATCTCATCCCCAACTACAGTCTCGGACAATGACACTGGTTCCTTTGACTGCAATATATGCTTAGATTCAGCACATGAACCTGTGGTGACTCTCTGTGGTCACCTGTACTGCTGGCCGTGCATTTACAAGTGGCTTCAGGTCCCATCCACCTCTGATGAACAATCCAACATGCCGCAAACTTGTCCTGTCTGTAAGGCTAACATCTCCCCTTCTTCAGTTGTTCCCCTCTACGGCCGAGGCATTTCCTCTTCAGATTCTGACGGAAAGAAACCTCAGTTGGATATGGTTGTACCACGTAGACCCCCACCTATGTCCAACACTTTGATAACTGCTACTACCGCCACATCAACGCCAAGACAGCAACTTCATCCAGATTTTTTGCAAAGGCATTCACAGTCCGTTCACGATCAGTACCACCCTGCTTATGGAGGTTATGCCACAAACTCAGAATCAGCTTATCTCGGGGGTACAACGATGACAAATTTATCCAATCCAACGATTGGAATGGTTGGAGAGTTTGTGTTTGCAAGGATGTTTGGGAGCTCAAACACCAGTTTGTTTACTTACCCGTATCTGAATTCGTACACCCCAAGCAGTCCTAGGATGAGAAGGCAGGAAGTAGAGCTTGACAAATCTCTCAATAGAGTAACATTCTTTCTTTTCTGCTGTTTTATGTTGTGCCTTCTCTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009611 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0051788 GO:0070647 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

263

Amino Acids

29.3

Weight (kDa)

6.05

Isoelectric Point (pI)

54.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	47 - 96	2e-07	Ring finger domain
zf-RING_5	PF14634	47 - 96	9.9e-07	zinc-RING finger domain
zf-C3HC4_3	PF13920	47 - 100	4.5e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	48 - 95	1.4e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	48 - 93	1.8e-08	RING-type zinc-finger
RING_XB3-XBAT31	PF24921	48 - 100	4.7e-08	E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-C3HC4_2	PF13923	48 - 77	1.5e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27470
fragaria_vesca	FvH4_3g09330 FvH4_3g09330 FvH4_3g20120 FvH4_3g20120
malus_domestica	MD00G1096000.v1.1 MD03G1226000.v1.1 MD05G1313600.v1.1 MD11G1245000.v1.1
prunus_persica	Prupe.4G046100_v2.0.a1 Prupe.4G046100_v2.0.a1 Prupe.4G183300_v2.0.a1 Prupe.4G183300_v2.0.a1
pyrus_communis	pycom03g17380 pycom05g29140 pycom11g21710
rosa_chinensis	RchiOBHm_Chr5g0008261 RchiOBHm_Chr5g0033921
rosa_laevigata	RLG00000031556 RLG00000033511
rosa_multiflora	Rmu_co8308883.1_g000001 Rmu_sc0001150.1_g000012 Rmu_sc0001387.1_g000006 Rmu_sc0007228.1_g000004
rosa_roxburghii	Rroxscaffold_1G00046290 Rroxscaffold_1G00068210
rosa_rugosa	Rorug04G0434700 Rorug04G0434700 Rorug05G0143400
rosa_samantha	Rh2AG319600 Rh5AG063300 Rh5AG234200 Rh5BG059500 Rh5BG234800 Rh5CG070100 Rh5CG265100 Rh5DG059200 Rh5DG241700
rosa_wichuraiana	Rw5G005760 Rw5G021510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccI	GTMKAC	1 cut(s)	391
AciI	CCGC	2 cut(s)	266, 432
AcoI	YGGCCR	2 cut(s)	206, 325
AcsI	RAATTY	3 cut(s)	88, 574, 670
AcuI	CTGAAG	3 cut(s)	212, 291, 327
AfaI	GTAC	5 cut(s)	200, 384, 506, 562, 677
AfiI	CCNNNNNNNGG	4 cut(s)	19, 323, 518, 593
AgsI	TTSAA	1 cut(s)	93
AjuI	GAANNNNNNNTTGG	2 cut(s)	353, 385
AluBI	AGCT	3 cut(s)	548, 636, 718
AluI	AGCT	3 cut(s)	548, 636, 718
Alw21I	GWGCWC	1 cut(s)	638
Alw26I	GTCTC	2 cut(s)	38, 118
AlwNI	CAGNNNCTG	1 cut(s)	350
Ama87I	CYCGRG	1 cut(s)	554
AoxI	GGCC	2 cut(s)	206, 325
ApoI	RAATTY	3 cut(s)	88, 574, 670
AspA2I	CCTAGG	1 cut(s)	692
AspS9I	GGNCC	1 cut(s)	232
AsuHPI	GGTGA	2 cut(s)	185, 190
AvaI	CYCGRG	1 cut(s)	554
AvaII	GGWCC	1 cut(s)	232
AvrII	CCTAGG	1 cut(s)	692
BanII	GRGCYC	1 cut(s)	638
Bbv12I	GWGCWC	1 cut(s)	638
BccI	CCATC	1 cut(s)	244
BceAI	ACGGC	2 cut(s)	193, 340
BcoDI	GTCTC	2 cut(s)	38, 118
BfaI	CTAG	1 cut(s)	693
BfmI	CTRYAG	1 cut(s)	108
BisI	GCNGC	1 cut(s)	266
BlnI	CCTAGG	1 cut(s)	692
BlsI	GCNGC	1 cut(s)	267
Bme18I	GGWCC	1 cut(s)	232
BmeT110I	CYCGRG	1 cut(s)	554
BmgT120I	GGNCC	1 cut(s)	232
BmiI	GGNNCC	2 cut(s)	133, 234
BsaAI	YACGTR	1 cut(s)	389
BsaJI	CCNNGG	2 cut(s)	328, 692
BsaXI	ACNNNNNCTCC	2 cut(s)	597, 627
Bsc4I	CCNNNNNNNGG	4 cut(s)	19, 323, 518, 593
Bse1I	ACTGG	2 cut(s)	133, 645
BseDI	CCNNGG	2 cut(s)	328, 692
BseGI	GGATG	5 cut(s)	98, 236, 460, 630, 702
BseLI	CCNNNNNNNGG	4 cut(s)	19, 323, 518, 593
BseMII	CTCAG	3 cut(s)	33, 380, 552
BseNI	ACTGG	2 cut(s)	133, 645
BseX3I	CGGCCG	1 cut(s)	325
Bsh1285I	CGRYCG	1 cut(s)	328
BshFI	GGCC	2 cut(s)	208, 327
BsiEI	CGRYCG	1 cut(s)	328
BsiHKAI	GWGCWC	1 cut(s)	638
BsiHKCI	CYCGRG	1 cut(s)	554
BslFI	GGGAC	1 cut(s)	218
BslI	CCNNNNNNNGG	4 cut(s)	19, 323, 518, 593
BsmAI	GTCTC	2 cut(s)	38, 118
BsmFI	GGGAC	1 cut(s)	218
BsmI	GAATGC	1 cut(s)	481
BsnI	GGCC	2 cut(s)	208, 327
BsoBI	CYCGRG	1 cut(s)	554
Bsp1286I	GDGCHC	1 cut(s)	638
Bsp143I	GATC	1 cut(s)	499
BspACI	CCGC	2 cut(s)	266, 432
BspANI	GGCC	2 cut(s)	208, 327
BspCNI	CTCAG	3 cut(s)	34, 379, 551
BspLI	GGNNCC	2 cut(s)	133, 234
BsrI	ACTGG	2 cut(s)	133, 645
BssECI	CCNNGG	2 cut(s)	328, 692
BssMI	GATC	1 cut(s)	499
BssT1I	CCWWGG	1 cut(s)	692
Bst4CI	ACNGT	2 cut(s)	112, 489
Bst6I	CTCTTC	1 cut(s)	346
BstAPI	GCANNNNNTGC	1 cut(s)	150
BstBAI	YACGTR	1 cut(s)	389
BstC8I	GCNNGC	1 cut(s)	206
BstDEI	CTNAG	4 cut(s)	42, 154, 366, 538
BstEII	GGTNACC	1 cut(s)	191
BstF5I	GGATG	5 cut(s)	98, 236, 460, 630, 702
BstKTI	GATC	1 cut(s)	502
BstMAI	GTCTC	2 cut(s)	38, 118
BstMBI	GATC	1 cut(s)	499
BstMCI	CGRYCG	1 cut(s)	328
BstMWI	GCNNNNNNNGC	4 cut(s)	150, 210, 431, 451
BstNSI	RCATGY	1 cut(s)	265
BstPI	GGTNACC	1 cut(s)	191
BstSFI	CTRYAG	1 cut(s)	108
BstZI	CGGCCG	1 cut(s)	325
BsuRI	GGCC	2 cut(s)	208, 327
BtsCI	GGATG	5 cut(s)	98, 236, 460, 630, 702
BtsIMutI	CAGTG	1 cut(s)	126
Cac8I	GCNNGC	1 cut(s)	206
CaiI	CAGNNNCTG	1 cut(s)	350
Cfr13I	GGNCC	1 cut(s)	232
Csp6I	GTAC	5 cut(s)	199, 383, 505, 561, 676
CviAII	CATG	2 cut(s)	167, 262
CviJI	RGCY	8 cut(s)	40, 208, 226, 290, 327, 548, 636, 718
CviKI_1	RGCY	8 cut(s)	40, 208, 226, 290, 327, 548, 636, 718
CviQI	GTAC	5 cut(s)	199, 383, 505, 561, 676
DdeI	CTNAG	4 cut(s)	42, 154, 366, 538
DpnI	GATC	1 cut(s)	501
DpnII	GATC	1 cut(s)	499
EaeI	YGGCCR	2 cut(s)	206, 325
EagI	CGGCCG	1 cut(s)	325
Eam1104I	CTCTTC	1 cut(s)	346
EarI	CTCTTC	1 cut(s)	346
Ecl136II	GAGCTC	1 cut(s)	636
EclXI	CGGCCG	1 cut(s)	325
Eco130I	CCWWGG	1 cut(s)	692
Eco24I	GRGCYC	1 cut(s)	638
Eco47I	GGWCC	1 cut(s)	232
Eco52I	CGGCCG	1 cut(s)	325
Eco53kI	GAGCTC	1 cut(s)	636
Eco57I	CTGAAG	3 cut(s)	212, 291, 327
Eco88I	CYCGRG	1 cut(s)	554
Eco91I	GGTNACC	1 cut(s)	191
EcoICRI	GAGCTC	1 cut(s)	636
EcoO109I	RGGNCCY	1 cut(s)	232
EcoO65I	GGTNACC	1 cut(s)	191
EcoRI	GAATTC	2 cut(s)	88, 670
EcoT14I	CCWWGG	1 cut(s)	692
EcoT38I	GRGCYC	1 cut(s)	638
ErhI	CCWWGG	1 cut(s)	692
FaeI	CATG	2 cut(s)	170, 265
FalI	AAGNNNNNCTT	2 cut(s)	702, 734
FaqI	GGGAC	1 cut(s)	218
FatI	CATG	2 cut(s)	166, 261
FblI	GTMKAC	1 cut(s)	391
Fnu4HI	GCNGC	1 cut(s)	266
FokI	GGATG	5 cut(s)	85, 223, 447, 637, 709
FriOI	GRGCYC	1 cut(s)	638
Fsp4HI	GCNGC	1 cut(s)	266
FspBI	CTAG	1 cut(s)	693
GluI	GCNGC	1 cut(s)	266
HaeIII	GGCC	2 cut(s)	208, 327
Hin1II	CATG	2 cut(s)	170, 265
HinfI	GANTC	4 cut(s)	158, 181, 347, 542
HphI	GGTGA	2 cut(s)	185, 190
Hpy166II	GTNNAC	4 cut(s)	392, 496, 654, 678
Hpy188I	TCNGA	7 cut(s)	118, 247, 346, 352, 541, 669, 788
Hpy188III	TCNNGA	3 cut(s)	79, 464, 497
Hpy8I	GTNNAC	4 cut(s)	392, 496, 654, 678
HpyAV	CCTTC	3 cut(s)	312, 696, 785
HpyCH4III	ACNGT	2 cut(s)	112, 489
HpyCH4IV	ACGT	1 cut(s)	388
HpyCH4V	TGCA	4 cut(s)	144, 213, 475, 620
HpyF10VI	GCNNNNNNNGC	4 cut(s)	150, 210, 431, 451
HpyF3I	CTNAG	4 cut(s)	42, 154, 366, 538
HpySE526I	ACGT	1 cut(s)	388
Hsp92II	CATG	2 cut(s)	170, 265
Kzo9I	GATC	1 cut(s)	499
LmnI	GCTCC	1 cut(s)	633
MaeI	CTAG	1 cut(s)	693
MaeII	ACGT	1 cut(s)	388
MaeIII	GTNAC	3 cut(s)	178, 191, 739
MalI	GATC	1 cut(s)	501
MboI	GATC	1 cut(s)	499
MboII	GAAGA	2 cut(s)	297, 333
MhlI	GDGCHC	1 cut(s)	638
MluCI	AATT	3 cut(s)	88, 574, 670
MlyI	GAGTC	1 cut(s)	175
MmeI	TCCRAC	6 cut(s)	43, 282, 351, 432, 583, 611
MnlI	CCTC	7 cut(s)	23, 253, 323, 329, 349, 375, 515
Mva1269I	GAATGC	1 cut(s)	481
MwoI	GCNNNNNNNGC	4 cut(s)	150, 210, 431, 451
NdeII	GATC	1 cut(s)	499
NlaIII	CATG	2 cut(s)	170, 265
NlaIV	GGNNCC	2 cut(s)	133, 234
NmeAIII	GCCGAG	1 cut(s)	353
NmuCI	GTSAC	2 cut(s)	178, 191
NspI	RCATGY	1 cut(s)	265
PctI	GAATGC	1 cut(s)	481
PfeI	GAWTC	3 cut(s)	158, 347, 542
PflMI	CCANNNNNTGG	1 cut(s)	593
PkrI	GCNGC	1 cut(s)	267
PleI	GAGTC	1 cut(s)	175
PpsI	GAGTC	1 cut(s)	175
Ppu21I	YACGTR	1 cut(s)	389
PpuMI	RGGWCCY	1 cut(s)	232
Psp124BI	GAGCTC	1 cut(s)	638
Psp5II	RGGWCCY	1 cut(s)	232
PspEI	GGTNACC	1 cut(s)	191
PspN4I	GGNNCC	2 cut(s)	133, 234
PspPI	GGNCC	1 cut(s)	232
PspPPI	RGGWCCY	1 cut(s)	232
PstNI	CAGNNNCTG	1 cut(s)	350
RsaI	GTAC	5 cut(s)	200, 384, 506, 562, 677
RsaNI	GTAC	5 cut(s)	199, 383, 505, 561, 676
SacI	GAGCTC	1 cut(s)	638
SatI	GCNGC	1 cut(s)	266
Sau3AI	GATC	1 cut(s)	499
Sau96I	GGNCC	1 cut(s)	232
SchI	GAGTC	1 cut(s)	175
SduI	GDGCHC	1 cut(s)	638
SfcI	CTRYAG	1 cut(s)	108
SinI	GGWCC	1 cut(s)	232
Sse9I	AATT	3 cut(s)	88, 574, 670
SsiI	CCGC	2 cut(s)	266, 432
SspMI	CTAG	1 cut(s)	693
SstI	GAGCTC	1 cut(s)	638
StyI	CCWWGG	1 cut(s)	692
TaaI	ACNGT	2 cut(s)	112, 489
TaiI	ACGT	1 cut(s)	391
TaqII	GACCGA	1 cut(s)	64
TasI	AATT	3 cut(s)	88, 574, 670
TatI	WGTACW	1 cut(s)	198
TauI	GCSGC	1 cut(s)	268
TfiI	GAWTC	3 cut(s)	158, 347, 542
TscAI	CASTG	1 cut(s)	133
TseFI	GTSAC	2 cut(s)	178, 191
Tsp45I	GTSAC	2 cut(s)	178, 191
TspDTI	ATGAA	3 cut(s)	183, 264, 449
TspGWI	ACGGA	2 cut(s)	369, 481
TspRI	CASTG	1 cut(s)	133
Van91I	CCANNNNNTGG	1 cut(s)	593
VpaK11BI	GGWCC	1 cut(s)	232
XapI	RAATTY	3 cut(s)	88, 574, 670
XceI	RCATGY	1 cut(s)	265
XcmI	CCANNNNNNNNNTGG	1 cut(s)	515
XmaJI	CCTAGG	1 cut(s)	692
XmiI	GTMKAC	1 cut(s)	391
XspI	CTAG	1 cut(s)	693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.