Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008319

Physical Location & Seq

Genomic Coordinates Reverse (-)

31162779 .. 31164991

Gene Structure 2213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1226200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGGCTACAGTCCTCGACTCCGTCGTCGCTCCCCGATCCTCCGCTCTCCCTTCTTCAGCCGCGCTCTCTCCGATCTCGGCTCCCTCCTCGCCCTCGTCCTTGTCCGCCGGCGCTCGGTTGCCTCGCTACAGAGGCCTCAAGGTGGGATCGGCTCTCCTAACTCGCTCCGCCAGATCGCTGAGTTACGGTCCTGCTTCCGGACGAGCGAGCCGCCGTGGTGGTCTCGTCGTCTGTGAGGCTCAGGACACCGCTGTCGAAGTGCCTGCTGTTGGTGATGCAACATGGCAGTCACTTGTCCTTGATTCTGAAACCCCTGTTTTGGTTGAATTCTGGGCTCCGTGGTGCGGGCCTTGCCGTATGATCCACCCTATAATTGATGAACTGGCAAAGCAATATGTTGGGAAGCTCAAATGCTACAAAGTTAACACTGATGAGAGCTCGTCAATTGCAACCCAGTATGGGATTCGAAGTATTCCAACTGTCATCATATTCAAGGGTGGCGAAAAAAAGGATGCCATTATTGGTGCTGTTCCCAAATCCACATTAACCACCAGCATAGAGAAGTTCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0004857 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006950 GO:0006979 GO:0008047 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0015035 GO:0015036 GO:0016020 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019222 GO:0019725 GO:0019904 GO:0030234 GO:0030312 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0033554 GO:0034357 GO:0034599 GO:0042221 GO:0042592 GO:0042651 GO:0043085 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051716 GO:0055035 GO:0055114 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071944 GO:0080090 GO:0097237 GO:0098754 GO:0098772 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko04621 ko05418 map04621 map05418

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

191

Amino Acids

20.01

Weight (kDa)

9.27

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	89 - 187	6.4e-30	Thioredoxin
Thioredoxin_2	PF13098	102 - 183	1.2e-08	Thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008319)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15360
fragaria_vesca	FvH4_3g09113 FvH4_3g20140 FvH4_4g22691
malus_domestica	MD03G1226200.v1.1 MD11G1244900.v1.1
prunus_persica	Prupe.4G183200_v2.0.a1
pyrus_communis	pycom03g17390 pycom11g21690
rosa_chinensis	RchiOBHm_Chr2g0145041 RchiOBHm_Chr5g0033941
rosa_laevigata	RLG00000033512
rosa_multiflora	Rmu_sc0001150.1_g000008 Rmu_sc0040826.1_g000001
rosa_roxburghii	Rroxscaffold_1G00046280
rosa_rugosa	Rorug05G0143500 Rorug05G0143600
rosa_samantha	Rh5AG234500 Rh5BG235000 Rh5CG265200 Rh5DG241900 Rh6BG019400
rosa_wichuraiana	Rw5G021500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	23, 251
AccBSI	CCGCTC	1 cut(s)	44
AccII	CGCG	1 cut(s)	62
AccIII	TCCGGA	1 cut(s)	197
AciI	CCGC	7 cut(s)	42, 60, 105, 168, 211, 249, 345
AclWI	GGATC	3 cut(s)	30, 154, 355
AcsI	RAATTY	1 cut(s)	326
AcuI	CTGAAG	1 cut(s)	39
AfiI	CCNNNNNNNGG	7 cut(s)	114, 142, 197, 269, 319, 344, 459
AgsI	TTSAA	2 cut(s)	326, 493
AluBI	AGCT	2 cut(s)	406, 438
AluI	AGCT	2 cut(s)	406, 438
Alw21I	GWGCWC	1 cut(s)	440
Alw26I	GTCTC	1 cut(s)	227
AlwI	GGATC	3 cut(s)	30, 154, 355
Aor13HI	TCCGGA	1 cut(s)	197
AoxI	GGCC	2 cut(s)	133, 347
ApoI	RAATTY	1 cut(s)	326
AspLEI	GCGC	2 cut(s)	64, 113
AspS9I	GGNCC	2 cut(s)	188, 347
AsuHPI	GGTGA	1 cut(s)	284
AsuII	TTCGAA	1 cut(s)	466
AvaII	GGWCC	1 cut(s)	188
BanII	GRGCYC	2 cut(s)	337, 440
Bbv12I	GWGCWC	1 cut(s)	440
BceAI	ACGGC	2 cut(s)	198, 339
BcgI	CGANNNNNNTGC	2 cut(s)	245, 279
BcoDI	GTCTC	1 cut(s)	227
BfmI	CTRYAG	2 cut(s)	6, 127
BfoI	RGCGCY	1 cut(s)	114
BisI	GCNGC	2 cut(s)	60, 211
BlsI	GCNGC	2 cut(s)	61, 212
Bme18I	GGWCC	1 cut(s)	188
BmgT120I	GGNCC	2 cut(s)	188, 347
BmiI	GGNNCC	2 cut(s)	81, 336
BmrI	ACTGGG	1 cut(s)	448
BmsI	GCATC	2 cut(s)	265, 502
BmuI	ACTGGG	1 cut(s)	448
Bpu10I	CCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	1 cut(s)	466
BpuEI	CTTGAG	1 cut(s)	122
BsaI	GGTCTC	1 cut(s)	227
BsaJI	CCNNGG	2 cut(s)	214, 338
BsaWI	WCCGGW	1 cut(s)	197
Bsc4I	CCNNNNNNNGG	7 cut(s)	114, 142, 197, 269, 319, 344, 459
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	2 cut(s)	387, 454
BseAI	TCCGGA	1 cut(s)	197
BseDI	CCNNGG	2 cut(s)	214, 338
BseGI	GGATG	1 cut(s)	517
BseLI	CCNNNNNNNGG	7 cut(s)	114, 142, 197, 269, 319, 344, 459
BseMII	CTCAG	2 cut(s)	170, 254
BseNI	ACTGG	2 cut(s)	387, 454
BseRI	GAGGAG	1 cut(s)	76
Bsh1236I	CGCG	1 cut(s)	62
BshFI	GGCC	2 cut(s)	135, 349
BsiHKAI	GWGCWC	1 cut(s)	440
BsiSI	CCGG	2 cut(s)	108, 198
BslI	CCNNNNNNNGG	7 cut(s)	114, 142, 197, 269, 319, 344, 459
BsmAI	GTCTC	1 cut(s)	227
BsnI	GGCC	2 cut(s)	135, 349
Bso31I	GGTCTC	1 cut(s)	227
Bsp119I	TTCGAA	1 cut(s)	466
Bsp1286I	GDGCHC	2 cut(s)	337, 440
Bsp13I	TCCGGA	1 cut(s)	197
Bsp143I	GATC	5 cut(s)	35, 72, 146, 173, 360
BspACI	CCGC	7 cut(s)	42, 60, 105, 168, 211, 249, 345
BspANI	GGCC	2 cut(s)	135, 349
BspCNI	CTCAG	2 cut(s)	171, 253
BspEI	TCCGGA	1 cut(s)	197
BspFNI	CGCG	1 cut(s)	62
BspLI	GGNNCC	2 cut(s)	81, 336
BspPI	GGATC	3 cut(s)	30, 154, 355
BspT104I	TTCGAA	1 cut(s)	466
BspTNI	GGTCTC	1 cut(s)	227
BsrBI	CCGCTC	1 cut(s)	44
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	2 cut(s)	387, 454
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	2 cut(s)	214, 338
BssMI	GATC	5 cut(s)	35, 72, 146, 173, 360
Bst4CI	ACNGT	3 cut(s)	10, 188, 481
BstBI	TTCGAA	1 cut(s)	466
BstC8I	GCNNGC	4 cut(s)	109, 208, 264, 347
BstDEI	CTNAG	2 cut(s)	179, 240
BstDSI	CCRYGG	2 cut(s)	214, 338
BstF5I	GGATG	1 cut(s)	517
BstFNI	CGCG	1 cut(s)	62
BstH2I	RGCGCY	1 cut(s)	114
BstHHI	GCGC	2 cut(s)	64, 113
BstKTI	GATC	5 cut(s)	38, 75, 149, 176, 363
BstMAI	GTCTC	1 cut(s)	227
BstMBI	GATC	5 cut(s)	35, 72, 146, 173, 360
BstMWI	GCNNNNNNNGC	2 cut(s)	132, 351
BstSFI	CTRYAG	2 cut(s)	6, 127
BstUI	CGCG	1 cut(s)	62
BsuRI	GGCC	2 cut(s)	135, 349
BtgI	CCRYGG	2 cut(s)	214, 338
BtsCI	GGATG	1 cut(s)	517
BtsIMutI	CAGTG	1 cut(s)	426
Cac8I	GCNNGC	4 cut(s)	109, 208, 264, 347
CfoI	GCGC	2 cut(s)	64, 113
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	2 cut(s)	188, 347
CviAII	CATG	1 cut(s)	282
DdeI	CTNAG	2 cut(s)	179, 240
DpnI	GATC	5 cut(s)	37, 74, 148, 175, 362
DpnII	GATC	5 cut(s)	35, 72, 146, 173, 360
DrdI	GACNNNNNNGTC	2 cut(s)	23, 251
DseDI	GACNNNNNNGTC	2 cut(s)	23, 251
EciI	GGCGGA	2 cut(s)	94, 157
Ecl136II	GAGCTC	1 cut(s)	438
Eco147I	AGGCCT	1 cut(s)	135
Eco24I	GRGCYC	2 cut(s)	337, 440
Eco31I	GGTCTC	1 cut(s)	227
Eco47I	GGWCC	1 cut(s)	188
Eco53kI	GAGCTC	1 cut(s)	438
Eco57I	CTGAAG	1 cut(s)	39
EcoICRI	GAGCTC	1 cut(s)	438
EcoRI	GAATTC	1 cut(s)	326
EcoT38I	GRGCYC	2 cut(s)	337, 440
FaeI	CATG	1 cut(s)	285
FaiI	YATR	7 cut(s)	283, 359, 371, 396, 459, 488, 557
FatI	CATG	1 cut(s)	281
FauI	CCCGC	1 cut(s)	338
Fnu4HI	GCNGC	2 cut(s)	60, 211
FokI	GGATG	1 cut(s)	524
FriOI	GRGCYC	2 cut(s)	337, 440
Fsp4HI	GCNGC	2 cut(s)	60, 211
GlaI	GCGC	2 cut(s)	63, 112
GluI	GCNGC	2 cut(s)	60, 211
HaeII	RGCGCY	1 cut(s)	114
HaeIII	GGCC	2 cut(s)	135, 349
HapII	CCGG	2 cut(s)	108, 198
HhaI	GCGC	2 cut(s)	64, 113
Hin1II	CATG	1 cut(s)	285
Hin6I	GCGC	2 cut(s)	62, 111
HinP1I	GCGC	2 cut(s)	62, 111
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HinfI	GANTC	3 cut(s)	17, 302, 463
HpaI	GTTAAC	1 cut(s)	424
HpaII	CCGG	2 cut(s)	108, 198
HphI	GGTGA	1 cut(s)	284
Hpy166II	GTNNAC	1 cut(s)	424
Hpy188I	TCNGA	2 cut(s)	72, 307
Hpy188III	TCNNGA	2 cut(s)	198, 242
Hpy8I	GTNNAC	1 cut(s)	424
Hpy99I	CGWCG	3 cut(s)	26, 29, 230
HpyAV	CCTTC	1 cut(s)	60
HpyCH4III	ACNGT	3 cut(s)	10, 188, 481
HpyCH4V	TGCA	2 cut(s)	278, 449
HpyF10VI	GCNNNNNNNGC	2 cut(s)	132, 351
HpyF3I	CTNAG	2 cut(s)	179, 240
Hsp92II	CATG	1 cut(s)	285
HspAI	GCGC	2 cut(s)	62, 111
Kpn2I	TCCGGA	1 cut(s)	197
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
KspAI	GTTAAC	1 cut(s)	424
Kzo9I	GATC	5 cut(s)	35, 72, 146, 173, 360
LmnI	GCTCC	4 cut(s)	34, 85, 170, 340
LweI	GCATC	2 cut(s)	265, 502
MaeIII	GTNAC	2 cut(s)	182, 288
MalI	GATC	5 cut(s)	37, 74, 148, 175, 362
MbiI	CCGCTC	1 cut(s)	44
MboI	GATC	5 cut(s)	35, 72, 146, 173, 360
MboII	GAAGA	1 cut(s)	45
MfeI	CAATTG	1 cut(s)	444
MhlI	GDGCHC	2 cut(s)	337, 440
MluCI	AATT	3 cut(s)	326, 372, 444
MlyI	GAGTC	1 cut(s)	11
MmeI	TCCRAC	1 cut(s)	500
MnlI	CCTC	9 cut(s)	23, 49, 94, 97, 103, 125, 132, 146, 229
MreI	CGCCGGCG	1 cut(s)	107
MroI	TCCGGA	1 cut(s)	197
MroNI	GCCGGC	1 cut(s)	107
MseI	TTAA	2 cut(s)	423, 545
MspA1I	CMGCKG	1 cut(s)	251
MspI	CCGG	2 cut(s)	108, 198
MunI	CAATTG	1 cut(s)	444
MvnI	CGCG	1 cut(s)	62
MwoI	GCNNNNNNNGC	2 cut(s)	132, 351
NaeI	GCCGGC	1 cut(s)	109
NdeII	GATC	5 cut(s)	35, 72, 146, 173, 360
NgoMIV	GCCGGC	1 cut(s)	107
NlaIII	CATG	1 cut(s)	285
NlaIV	GGNNCC	2 cut(s)	81, 336
NmeAIII	GCCGAG	1 cut(s)	56
NmuCI	GTSAC	1 cut(s)	288
NspV	TTCGAA	1 cut(s)	466
PceI	AGGCCT	1 cut(s)	135
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PdiI	GCCGGC	1 cut(s)	109
PfeI	GAWTC	2 cut(s)	302, 463
PflFI	GACNNNGTC	1 cut(s)	20
PkrI	GCNGC	2 cut(s)	61, 212
PleI	GAGTC	1 cut(s)	11
PpsI	GAGTC	1 cut(s)	11
Psp124BI	GAGCTC	1 cut(s)	440
PspN4I	GGNNCC	2 cut(s)	81, 336
PspPI	GGNCC	2 cut(s)	188, 347
PsyI	GACNNNGTC	1 cut(s)	20
SacI	GAGCTC	1 cut(s)	440
SaqAI	TTAA	2 cut(s)	423, 545
SatI	GCNGC	2 cut(s)	60, 211
Sau3AI	GATC	5 cut(s)	35, 72, 146, 173, 360
Sau96I	GGNCC	2 cut(s)	188, 347
SchI	GAGTC	1 cut(s)	11
SduI	GDGCHC	2 cut(s)	337, 440
SetI	ASST	3 cut(s)	144, 408, 440
SfaNI	GCATC	2 cut(s)	265, 502
SfcI	CTRYAG	2 cut(s)	6, 127
SfuI	TTCGAA	1 cut(s)	466
SgrAI	CRCCGGYG	1 cut(s)	107
SinI	GGWCC	1 cut(s)	188
SmlI	CTYRAG	1 cut(s)	137
SmoI	CTYRAG	1 cut(s)	137
Sse9I	AATT	3 cut(s)	326, 372, 444
SseBI	AGGCCT	1 cut(s)	135
SsiI	CCGC	7 cut(s)	42, 60, 105, 168, 211, 249, 345
SstI	GAGCTC	1 cut(s)	440
StuI	AGGCCT	1 cut(s)	135
TaaI	ACNGT	3 cut(s)	10, 188, 481
TaqI	TCGA	3 cut(s)	15, 255, 466
TasI	AATT	3 cut(s)	326, 372, 444
TauI	GCSGC	2 cut(s)	62, 213
TfiI	GAWTC	2 cut(s)	302, 463
Tru1I	TTAA	2 cut(s)	423, 545
Tru9I	TTAA	2 cut(s)	423, 545
TscAI	CASTG	1 cut(s)	433
TseFI	GTSAC	1 cut(s)	288
Tsp45I	GTSAC	1 cut(s)	288
TspDTI	ATGAA	1 cut(s)	393
TspGWI	ACGGA	2 cut(s)	10, 327
TspRI	CASTG	1 cut(s)	433
Tth111I	GACNNNGTC	1 cut(s)	20
VpaK11BI	GGWCC	1 cut(s)	188
XapI	RAATTY	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.