MD03G1260900.v1.1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014812
Physical Location & Seq
Forward (+)
34734594 .. 34734887
294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1260900.v1.1.491

Sequence Viewer

Length: 294 bp
ATGCGGTTCAACATACCCGTTCGCATTTTAGTTTTCTTCTTCCTCACTAGGTCCCTGTTTATTGGCATCGCACGTAGCCAAAAAATATTTGACATAACCAAATACGGTGCTGTTGGAGACGGACGAACCGATGATTCCCCGGCTTTTCTGAAGTCATGGGGTGACATATGTAACGGTGAGAATGACGGTGTACTGACTCTTCTTGTTCCAGAGGGCAAAACGTTCATGTTGCAACCTATTAAATTTGAAGGTCCATGCGAGCCAAAAAGTCTACTCTACATATTGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

98

Amino Acids

10.96

Weight (kDa)

8.66

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 271
AciI CCGC 1 cut(s) 4
AclI AACGTT 1 cut(s) 221
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 1 cut(s) 192
AgsI TTSAA 3 cut(s) 10, 248, 286
Alw26I GTCTC 1 cut(s) 111
ApoI RAATTY 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 51, 251
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 173, 188
AvaII GGWCC 2 cut(s) 51, 251
BarI GAAGNNNNNNTAC 2 cut(s) 183, 215
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 51, 251
BmgT120I GGNCC 2 cut(s) 51, 251
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 111
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 37
BspACI CCGC 1 cut(s) 4
BspLI GGNNCC 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 107, 176, 188
Bst6I CTCTTC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 260
BstMAI GTCTC 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 260
Cfr13I GGNCC 2 cut(s) 51, 251
Csp6I GTAC 1 cut(s) 191
CviAII CATG 3 cut(s) 156, 226, 255
CviJI RGCY 3 cut(s) 78, 143, 262
CviKI_1 RGCY 3 cut(s) 78, 143, 262
CviQI GTAC 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 51, 251
Eco57I CTGAAG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 3 cut(s) 159, 229, 258
FaiI YATR 8 cut(s) 14, 95, 157, 167, 169, 227, 256, 281
FaqI GGGAC 1 cut(s) 37
FatI CATG 3 cut(s) 155, 225, 254
FauNDI CATATG 1 cut(s) 167
FblI GTMKAC 1 cut(s) 271
FspBI CTAG 1 cut(s) 48
HapII CCGG 1 cut(s) 140
Hin1II CATG 3 cut(s) 159, 229, 258
HinfI GANTC 2 cut(s) 134, 196
HpaII CCGG 1 cut(s) 140
HphI GGTGA 2 cut(s) 173, 188
Hpy166II GTNNAC 2 cut(s) 191, 272
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 2 cut(s) 191, 272
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 3 cut(s) 107, 176, 188
HpyCH4IV ACGT 2 cut(s) 73, 221
HpyCH4V TGCA 1 cut(s) 232
HpySE526I ACGT 2 cut(s) 73, 221
Hsp92II CATG 3 cut(s) 159, 229, 258
LpnPI CCDG 3 cut(s) 68, 153, 222
LweI GCATC 1 cut(s) 75
MaeI CTAG 1 cut(s) 48
MaeII ACGT 2 cut(s) 73, 221
MaeIII GTNAC 2 cut(s) 161, 170
MboII GAAGA 3 cut(s) 28, 31, 191
MluCI AATT 1 cut(s) 242
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 2 cut(s) 53, 205
MseI TTAA 1 cut(s) 240
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 140
NciI CCSGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 167
NlaIII CATG 3 cut(s) 159, 229, 258
NlaIV GGNNCC 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 134
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Ppu21I YACGTR 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 51
Psp1406I AACGTT 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 53
PspPI GGNCC 2 cut(s) 51, 251
PspPPI RGGWCCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 240
Sau96I GGNCC 2 cut(s) 51, 251
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 140
SetI ASST 5 cut(s) 53, 76, 224, 238, 253
SfaNI GCATC 1 cut(s) 75
SinI GGWCC 2 cut(s) 51, 251
Sse9I AATT 1 cut(s) 242
SsiI CCGC 1 cut(s) 4
SspI AATATT 1 cut(s) 87
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 107, 176, 188
TaiI ACGT 2 cut(s) 76, 224
TasI AATT 1 cut(s) 242
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TseFI GTSAC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 135
VpaK11BI GGWCC 2 cut(s) 51, 251
XapI RAATTY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.